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ID PGBT9 preliminary; circular DNA; SYN; 5524 BP.
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AC U07646;
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DT 01-JUL-1994 (Rel. 9, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE Yeast/E.coli plasmid vector pGBT9 - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RP 1-5524
RC pGBT9 from ADH1 promoter & GAL4 & ADH1 terminator & TRP1 gene
RA Kitts P.A., Ledesma L.J.;
RT ;
RL Unpublished (1994).
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RN [2]
RP 1-5524
RC pBTM116 from ADH1 promoter/terminator & TRP1 gene & lexA gene
RC pGBT9 from ADH1 promoter/terminator & GAL4 & TRP1 gene
RC pGAD10 from ColE1 & GAL4 & LEU2 gene
RC pGAD424 from ADH1 promoter/terminator & GAL4 & LEU2 gene
RC [lambda ACT from lambda & LEU2 gene]
RA Bartel P.L., Chien C., Sternglanz R., Fields S.;
RT "Using the two-hybrid system to detect protein-protein interactions";
RL Cellular Interactions In Development 0:153-179(1993).
RL Hartley D., ed., Oxford University Press, Oxford.
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RN [3]
RP 1-5524
RC pGBT9
RA Kitts P.A.;
RT ;
RL Submitted (11-MAR-1994) by:
RL Kitts P.A., CLONTECH Laboratories, Inc.,
RL 4030 Fabian Way, Palo Alto, CA 94303, USA.
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CC NCBI gi: 470667
CC NM (pGBT9)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (CLONTECH)
CC HO (E.coli)
CC CP ()
CC FN (expression)(fusion)
CC SE ()
CC PA ()
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. yeast, ADH1 promoter SphI-HindIII
FT 2. yeast, GAL4 gene HindIII-EcoRI, activator domain
FT 3. yeast, ADH1 terminator PstI-SphI
FT 4. yeast, TRP1 gene NaeI-PvuII
FT 5. pBR322, ori/amp
FT 6. yeast 2 micron, ori
FT -> pGBT9 5524bp"
FT misc_binding 6..6
FT /note="SIT SphI"
FT promoter 6..411
FT /note="PRO yeast ADH1 gene"
FT misc_binding 411..411
FT /note="SIT HindIII"
FT promoter 411..870
FT /note="PRO yeast GAL4 bd"
FT misc_binding 643..643
FT /note="SIT unique XhoI"
FT misc_binding 701..701
FT /note="SIT unique HpaI"
FT misc_binding 870..892
FT /note="MCS unique EcoRI-SmaI-BamHI-SalI-PstI"
FT terminator 892..1100
FT /note="TER yeast ADH1 gene"
FT misc_binding 1100..1100
FT /note="SIT SphI"
FT misc_binding 1104..1104
FT /note="SIT unique NaeI"
FT CDS 1104..1900
FT /note="YEA yeast TRP1 gene"
FT misc_binding 1310..1310
FT /note="SIT HindIII"
FT misc_binding 1526..1526
FT /note="SIT unique BstXI"
FT misc_binding 1540..1540
FT /note="SIT unique EcoRV"
FT misc_binding 1738..1738
FT /note="SIT unique XbaI"
FT misc_binding 2111..2111
FT /note="SIT unique PvuII"
FT rep_origin 2300..2500
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 3100..3900
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amr)"
FT misc_binding 3301..3301
FT /note="SIT unique BglI"
FT misc_binding 3549..3549
FT /note="SIT unique PvuI"
FT misc_binding 3669..3669
FT /note="SIT unique ScaI"
FT misc_binding 4100..4100
FT /note="SIT unique AatII"
FT rep_origin 0..0
FT /note="ORI yeast 2 micron"
FT misc_binding 4600..4600
FT /note="SIT unique SnaBI"
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SQ Sequence 5524 BP; 1555 A; 1290 C; 1150 G; 1529 T; 0 other;
gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccgtcgacc
tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag
ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
tcgctcttat tgaccacacc tctaccggca tgccggcaag tgcacaaaca atacttaaat
aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca
cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct
gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg
aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg
taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag
tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca
ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca
tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat
ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta
atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt
cttttttcga ccgaattaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
gaaacctgtc gtgccagctg gattaatgaa tcggccaacg cgcggggaga ggcggtttgc
gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag cataaacacg
cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac
gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg
aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt ataggaactt
cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc
accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa
agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt
cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt
actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg
aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa
ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat
ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct
tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata
gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag
actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa
tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa
aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga
aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt
ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt
cgtt
//