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ID   PGBT9      preliminary; circular DNA; SYN; 5524 BP.
XX
AC   U07646;
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DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Yeast/E.coli plasmid vector pGBT9 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-5524
RC   pGBT9 from ADH1 promoter & GAL4 & ADH1 terminator & TRP1 gene
RA   Kitts P.A., Ledesma L.J.;
RT   ;
RL   Unpublished (1994).
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RN   [2]
RP   1-5524
RC   pBTM116 from ADH1 promoter/terminator & TRP1 gene & lexA gene
RC   pGBT9 from ADH1 promoter/terminator & GAL4 & TRP1 gene
RC   pGAD10 from ColE1 & GAL4 & LEU2 gene
RC   pGAD424 from ADH1 promoter/terminator & GAL4 & LEU2 gene
RC   [lambda ACT from lambda & LEU2 gene]
RA   Bartel P.L., Chien C., Sternglanz R., Fields S.;
RT   "Using the two-hybrid system to detect protein-protein interactions";
RL   Cellular Interactions In Development 0:153-179(1993).
RL   Hartley D., ed., Oxford University Press, Oxford.
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RN   [3]
RP   1-5524
RC   pGBT9
RA   Kitts P.A.;
RT   ;
RL   Submitted (11-MAR-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
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CC   NCBI gi: 470667
CC   NM (pGBT9)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(fusion)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. yeast, ADH1 promoter SphI-HindIII
FT                   2. yeast, GAL4 gene HindIII-EcoRI, activator domain
FT                   3. yeast, ADH1 terminator PstI-SphI
FT                   4. yeast, TRP1 gene NaeI-PvuII
FT                   5. pBR322, ori/amp
FT                   6. yeast 2 micron, ori
FT                   -> pGBT9 5524bp"
FT   misc_binding    6..6
FT                   /note="SIT SphI"
FT   promoter        6..411
FT                   /note="PRO yeast ADH1 gene"
FT   misc_binding    411..411
FT                   /note="SIT HindIII"
FT   promoter        411..870
FT                   /note="PRO yeast GAL4 bd"
FT   misc_binding    643..643
FT                   /note="SIT unique XhoI"
FT   misc_binding    701..701
FT                   /note="SIT unique HpaI"
FT   misc_binding    870..892
FT                   /note="MCS unique EcoRI-SmaI-BamHI-SalI-PstI"
FT   terminator      892..1100
FT                   /note="TER yeast ADH1 gene"
FT   misc_binding    1100..1100
FT                   /note="SIT SphI"
FT   misc_binding    1104..1104
FT                   /note="SIT unique NaeI"
FT   CDS             1104..1900
FT                   /note="YEA yeast TRP1 gene"
FT   misc_binding    1310..1310
FT                   /note="SIT HindIII"
FT   misc_binding    1526..1526
FT                   /note="SIT unique BstXI"
FT   misc_binding    1540..1540
FT                   /note="SIT unique EcoRV"
FT   misc_binding    1738..1738
FT                   /note="SIT unique XbaI"
FT   misc_binding    2111..2111
FT                   /note="SIT unique PvuII"
FT   rep_origin      2300..2500
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             3100..3900
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amr)"
FT   misc_binding    3301..3301
FT                   /note="SIT unique BglI"
FT   misc_binding    3549..3549
FT                   /note="SIT unique PvuI"
FT   misc_binding    3669..3669
FT                   /note="SIT unique ScaI"
FT   misc_binding    4100..4100
FT                   /note="SIT unique AatII"
FT   rep_origin      0..0
FT                   /note="ORI yeast 2 micron"
FT   misc_binding    4600..4600
FT                   /note="SIT unique SnaBI"
XX
SQ   Sequence 5524 BP; 1555 A; 1290 C; 1150 G; 1529 T; 0 other;
     gcttgcatgc aacttctttt cttttttttt cttttctctc tcccccgttg ttgtctcacc
     atatccgcaa tgacaaaaaa aatgatggaa gacactaaag gaaaaaatta acgacaaaga
     cagcaccaac agatgtcgtt gttccagagc tgatgagggg tatcttcgaa cacacgaaac
     tttttccttc cttcattcac gcacactact ctctaatgag caacggtata cggccttcct
     tccagttact tgaatttgaa ataaaaaaag tttgccgctt tgctatcaag tataaataga
     cctgcaatta ttaatctttt gtttcctcgt cattgttctc gttccctttc ttccttgttt
     ctttttctgc acaatatttc aagctatacc aagcatacaa tcaactccaa gcttgaagca
     agcctcctga aagatgaagc tactgtcttc tatcgaacaa gcatgcgata tttgccgact
     taaaaagctc aagtgctcca aagaaaaacc gaagtgcgcc aagtgtctga agaacaactg
     ggagtgtcgc tactctccca aaaccaaaag gtctccgctg actagggcac atctgacaga
     agtggaatca aggctagaaa gactggaaca gctatttcta ctgatttttc ctcgagaaga
     ccttgacatg attttgaaaa tggattcttt acaggatata aaagcattgt taacaggatt
     atttgtacaa gataatgtga ataaagatgc cgtcacagat agattggctt cagtggagac
     tgatatgcct ctaacattga gacagcatag aataagtgcg acatcatcat cggaagagag
     tagtaacaaa ggtcaaagac agttgactgt atcgccggaa ttcccgggga tccgtcgacc
     tgcagccaag ctaattccgg gcgaatttct tatgatttat gatttttatt attaaataag
     ttataaaaaa aataagtgta tacaaatttt aaagtgactc ttaggtttta aaacgaaaat
     tcttgttctt gagtaactct ttcctgtagg tcaggttgct ttctcaggta tagcatgagg
     tcgctcttat tgaccacacc tctaccggca tgccggcaag tgcacaaaca atacttaaat
     aaatactact cagtaataac ctatttctta gcatttttga cgaaatttgc tattttgtta
     gagtctttta caccatttgt ctccacacct ccgcttacat caacaccaat aacgccattt
     aatctaagcg catcaccaac attttctggc gtcagtccac cagctaacat aaaatgtaag
     ctttcggggc tctcttgcct tccaacccag tcagaaatcg agttccaatc caaaagttca
     cctgtcccac ctgcttctga atcaaacaag ggaataaacg aatgaggttt ctgtgaagct
     gcactgagta gtatgttgca gtcttttgga aatacgagtc ttttaataac tggcaaaccg
     aggaactctt ggtattcttg ccacgactca tctccatgca gttggacgat atcaatgccg
     taatcattga ccagagccaa aacatcctcc ttaggttgat tacgaaacac gccaaccaag
     tatttcggag tgcctgaact atttttatat gcttttacaa gacttgaaat tttccttgca
     ataaccgggt caattgttct ctttctattg ggcacacata taatacccag caagtcagca
     tcggaatcta gagcacattc tgcggcctct gtgctctgca agccgcaaac tttcaccaat
     ggaccagaac tacctgtgaa attaataaca gacatactcc aagctgcctt tgtgtgctta
     atcacgtata ctcacgtgct caatagtcac caatgccctc cctcttggcc ctctcctttt
     cttttttcga ccgaattaat tcgtaatcat ggtcatagct gtttcctgtg tgaaattgtt
     atccgctcac aattccacac aacatacgag ccggaagcat aaagtgtaaa gcctggggtg
     cctaatgagt gaggtaactc acattaattg cgttgcgctc actgcccgct ttccagtcgg
     gaaacctgtc gtgccagctg gattaatgaa tcggccaacg cgcggggaga ggcggtttgc
     gtattgggcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc
     ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata
     acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg
     cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct
     caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa
     gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc
     tcccttcggg aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt
     aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg
     ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg
     cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct acagagttct
     tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc tgcgctctgc
     tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa caaaccaccg
     ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa aaaggatctc
     aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa aactcacgtt
     aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt ttaaattaaa
     aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac agttaccaat
     gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc atagttgcct
     gactccccgt cgtgtagata actacgatac gggagggctt accatctggc cccagtgctg
     caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata aaccagccag
     ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc cagtctatta
     attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc aacgttgttg
     ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca ttcagctccg
     gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa gcggttagct
     ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca ctcatggtta
     tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt tctgtgactg
     gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt tgctcttgcc
     cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg ctcatcattg
     gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga tccagttcga
     tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc agcgtttctg
     ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg acacggaaat
     gttgaatact catactcttc ctttttcaat attattgaag catttatcag ggttattgtc
     tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg gttccgcgca
     catttccccg aaaagtgcca cctgacgtct aagaaaccat tattatcatg acattaacct
     ataaaaatag gcgtatcacg aggccctttc gtctcgcgcg tttcggtgat gacggtgaaa
     acctctgaca catgcagctc ccggagacgg tcacagcttg tctgtaagcg gatgccggga
     gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg gtgtcggggc tggcttaact
     atgcggcatc agagcagatt gtactgagag tgcaccataa cgcatttaag cataaacacg
     cactatgccg ttcttctcat gtatatatat atacaggcaa cacgcagata taggtgcgac
     gtgaacagtg agctgtatgt gcgcagctcg cgttgcattt tcggaagcgc tcgttttcgg
     aaacgctttg aagttcctat tccgaagttc ctattctcta gctagaaagt ataggaactt
     cagagcgctt ttgaaaacca aaagcgctct gaagacgcac tttcaaaaaa ccaaaaacgc
     accggactgt aacgagctac taaaatattg cgaataccgc ttccacaaac attgctcaaa
     agtatctctt tgctatatat ctctgtgcta tatccctata taacctaccc atccaccttt
     cgctccttga acttgcatct aaactcgacc tctacatttt ttatgtttat ctctagtatt
     actctttaga caaaaaaatt gtagtaagaa ctattcatag agtgaatcga aaacaatacg
     aaaatgtaaa catttcctat acgtagtata tagagacaaa atagaagaaa ccgttcataa
     ttttctgacc aatgaagaat catcaacgct atcactttct gttcacaaag tatgcgcaat
     ccacatcggt atagaatata atcggggatg cctttatctt gaaaaaatgc acccgcagct
     tcgctagtaa tcagtaaacg cgggaagtgg agtcaggctt tttttatgga agagaaaata
     gacaccaaag tagccttctt ctaaccttaa cggacctaca gtgcaaaaag ttatcaagag
     actgcattat agagcgcaca aaggagaaaa aaagtaatct aagatgcttt gttagaaaaa
     tagcgctctc gggatgcatt tttgtagaac aaaaaagaag tatagattct ttgttggtaa
     aatagcgctc tcgcgttgca tttctgttct gtaaaaatgc agctcagatt ctttgtttga
     aaaattagcg ctctcgcgtt gcatttttgt tttacaaaaa tgaagcacag attcttcgtt
     ggtaaaatag cgctttcgcg ttgcatttct gttctgtaaa aatgcagctc agattctttg
     tttgaaaaat tagcgctctc gcgttgcatt tttgttctac aaaatgaagc acagatgctt
     cgtt
//