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ID   PGD56      preliminary; circular DNA; SYN; 4547 BP.
XX
AC   X67018;
XX
DT   23-SEP-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGD56 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4547
RC   pGD56 from pBR322
RA   Dandanell G.;
RT   "A multi and singlecopy promoter-cloning vector system for divergent
RT   promoters";
RL   Unpublished (1992).
XX
CC   NM (pGD56)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. E. coli, galK gene
FT                   3. lambda, tI terminator
FT                   -> pGD56 4547bp"
FT   misc_feature    1..617
FT                   /note="E. coli B-region"
FT   terminator      618..862
FT                   /note="TER lambda tI terminator"
FT   CDS             1049..2197
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   misc_feature    complement(2244..4540)
FT                   /note="from pBR322"
FT   CDS             complement(3469..4330)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4547 BP; 1165 A; 1147 C; 1150 G; 1085 T; 0 other;
     tcttctcgcc cgattctgtc ggcaaaagaa gcgcagaatt tcgatgctca acactatttt
     gcatccctga caccaggtgc tgcagcgtgg aatccttccc cgattaccct gcctgcgcaa
     cctgactttg ttgtcggccc ggcgcactca aggtgtaacg cataccacga ttcaggcggc
     ggtagatgcg gcaattatca agcgtaccaa caagcgccag tatattgccg tgatgcctgg
     tgagtatcag ggaacggtat atgtccctgc cgctccgggt ggaattactc tgtacggtac
     aggtgaaaaa ccgattgatg tgaagattgg gctttccctt gatggtggca tgagccctgc
     cgactggcgt cacgacgtca acccgctcgg caaatatatg ccaggtaaac cagcgtggta
     tatgtacgat agctgccaga gcaaacgcag cgacagtatc ggtgttctct gctctgcggt
     cttctggtca caaaacaatg gcctgcaact gcaaaatctg accatcgaaa actctctggg
     cgatggtaga tgcaggtaac catccgtgcg gtggcactgc gttactgatg gtgaccaggt
     acagattaac aacgtttctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tgcagtcact
     atgaatcaac tacttagatg gtattagtga cctgtaacag agcattagcg caaggtgatt
     tttgtcttct tgcgctaatt ttttgtcatc aaacctgtcg cactccagag aagcacaaag
     cctcgcaatc cagtgcaaag cttgggctgc aggtcgactc tagaggatcc ccgggcgagc
     tcgaattaga tcctaatcta gattaggtaa atttatggtt ggttatgaaa tgctggcaga
     gacccagcga gacctgaccg cagaacaggc agcagagcgt ttgcgcgcag tcagcgatat
     ccattttcgc gaatccggag tgtaagaaat gagtctgaaa gaaaaaacac aatctctgtt
     tgccaacgca tttggctacc ctgccactca caccattcag gcgcctggcc gcgtgaattt
     gattggtgaa cacaccgact acaacgacgg tttcgttctg ccctgcgcga ttgattatca
     aaccgtgatc agttgtgcac cacgcgatga ccgtaaagtt cgcgtgatgg cagccgatta
     tgaaaatcag ctcgacgagt tttccctcga tgcgcccatt gtcgcacatg aaaactatca
     atgggctaac tacgttcgtg gcgtggtgaa acatctgcaa ctgcgtaaca acagcttcgg
     cggcgtggac atggtgatca gcggcaatgt gccgcagggt gccgggttaa gttcttccgc
     ttcactggaa gtcgcggtcg gaaccgtatt gcagcagctt tatcatctgc cgctggacgg
     cgcacaaatc gcgcttaacg gtcaggaagc agaaaaccag tttgtaggct gtaactgcgg
     gatcatggat cagctaattt ccgcgctcgg caagaaagat catgccttgc tgatcgattg
     ccgctcactg gggaccaaag cagtttccat gcccaaaggt gtggctgtcg tcatcatcaa
     cagtaacttc aaacgtaccc tggttggcag cgaatacaac acccgtcgtg aacagtgcga
     aaccggtgcg cgtttcttcc agcagccagc cctgcgtgat gtcaccattg aagagttcaa
     cgctgttgcg catgaactgg acccgatcgt ggcaaaacgc gtgcgtcata tactgactga
     aaacgcccgc accgttgaag ctgccagcgc gctggagcaa ggcgacctga aacgtatggg
     cgagttgatg gcggagtctc atgcctctat gcgcgatgat ttcgaaatca ccgtgccgca
     aattgacact ctggtagaaa tcgtcaaagc tgtgattggc gacaaaggtg gcgtacgcat
     gaccggcggc ggatttggcg gctgtatcgt cgcgctgatc ccggaagagc tggtgcctgc
     cgtacagcaa gctgtcgctg aacaatatga agcaaaaaca ggtattaaag agacttttta
     cgtttgtaaa ccatcacaag gagcaggaca gtgctgaacg aaactcccgc actggcaccc
     gatggtcagc cgtaccgact gttctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc
     tgacacatgc agctcccgga gacggtcaca gcttgtctgt aagcggatgc cgggagcaga
     caagcccgtc agggcgcgtc agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag
     tcacgtagcg atagcggagt gtatactggc ttaactatgc ggcatcagag cagattgtac
     tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     tcaggcgctc ttccgcttcc tcgctcactg actcgctgcg ctcggtcgtt cggctgcggc
     gagcggtatc agctcactca aaggcggtaa tacggttatc cacagaatca ggggataacg
     caggaaagaa catgtgagca aaaggccagc aaaaggccag gaaccgtaaa aaggccgcgt
     tgctggcgtt tttccatagg ctccgccccc ctgacgagca tcacaaaaat cgacgctcaa
     gtcagaggtg gcgaaacccg acaggactat aaagatacca ggcgtttccc cctggaagct
     ccctcgtgcg ctctcctgtt ccgaccctgc cgcttaccgg atacctgtcc gcctttctcc
     cttcgggaag cgtggcgctt tctcatagct cacgctgtag gtatctcagt tcggtgtagg
     tcgttcgctc caagctgggc tgtgtgcacg aaccccccgt tcagcccgac cgctgcgcct
     tatccggtaa ctatcgtctt gagtccaacc cggtaagaca cgacttatcg ccactggcag
     cagccactgg taacaggatt agcagagcga ggtatgtagg cggtgctaca gagttcttga
     agtggtggcc taactacggc tacactagaa ggacagtatt tggtatctgc gctctgctga
     agccagttac cttcggaaaa agagttggta gctcttgatc cggcaaacaa accaccgctg
     gtagcggtgg tttttttgtt tgcaagcagc agattacgcg cagaaaaaaa ggatctcaag
     aagatccttt gatcttttct acggggtctg acgctcagtg gaacgaaaac tcacgttaag
     ggattttggt catgagatta tcaaaaagga tcttcaccta gatcctttta aattaaaaat
     gaagttttaa atcaatctaa agtatatatg agtaaacttg gtctgacagt taccaatgct
     taatcagtga ggcacctatc tcagcgatct gtctatttcg ttcatccata gttgcctgac
     tccccgtcgt gtagataact acgatacggg agggcttacc atctggcccc agtgctgcaa
     tgataccgcg agacccacgc tcaccggctc cagatttatc agcaataaac cagccagccg
     gaagggccga gcgcagaagt ggtcctgcaa ctttatccgc ctccatccag tctattaatt
     gttgccggga agctagagta agtagttcgc cagttaatag tttgcgcaac gttgttgcca
     ttgctgcagg catcgtggtg tcacgctcgt cgtttggtat ggcttcattc agctccggtt
     cccaacgatc aaggcgagtt acatgatccc ccatgttgtg caaaaaagcg gttagctcct
     tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt gttatcactc atggttatgg
     cagcactgca taattctctt actgtcatgc catccgtaag atgcttttct gtgactggtg
     agtactcaac caagtcattc tgagaatagt gtatgcggcg accgagttgc tcttgcccgg
     cgtcaacacg ggataatacc gcgccacata gcagaacttt aaaagtgctc atcattggaa
     aacgttcttc ggggcgaaaa ctctcaagga tcttaccgct gttgagatcc agttcgatgt
     aacccactcg tgcacccaac tgatcttcag catcttttac tttcaccagc gtttctgggt
     gagcaaaaac aggaaggcaa aatgccgcaa aaaagggaat aagggcgaca cggaaatgtt
     gaatactcat actcttcctt tttcaatatt attgaagcat ttatcagggt tattgtctca
     tgagcggata catatttgaa tgtatttaga aaaataaaca aataggggtt ccgcgcacat
     ttccccgaaa agtgccacct gacgtctaag aaaccattat tatcatgaca ttaacctata
     aaaataggcg tatcacgagg ccctttcgtc ttcaagaatt gggtacc
//