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ID   PGD57      preliminary; circular DNA; SYN; 7760 BP.
XX
AC   X67019;
XX
DT   23-SEP-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGD57 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-7760
RC   pGD57 from pBR322
RA   Dandanell G.;
RT   "A multi and singlecopy promoter-cloning vector system for divergent
RT   promoters";
RL   Unpublished (1992).
XX
CC   NM (pGD57)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA ()
CC   BR (pBR322)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. E. coli, galK gene
FT                   3. lambda, tI terminator
FT                   -> pGD57 7760bp"
FT   CDS             complement(928..4003)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   CDS             4262..5411
FT                   /note="GEN E. coli galactokinase gene (galK)"
FT   misc_feature    complement(5457..7753)
FT                   /note="from pBR322"
FT   CDS             complement(6682..7543)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 7760 BP; 1867 A; 2062 C; 2030 G; 1801 T; 0 other;
     tcttctcgcc cgattctgtc ggcaaaagaa gcgcagaatt tcgatgctca acactatttt
     gcatccctga caccaggtgc tgcagcgtgg aatccttccc cgattaccct gcctgcgcaa
     cctgactttg ttgtcggccc ggcgcactca aggtgtaacg cataccacga ttcaggcggc
     ggtagatgcg gcaattatca agcgtaccaa caagcgccag tatattgccg tgatgcctgg
     tgagtatcag ggaacggtat atgtccctgc cgctccgggt ggaattactc tgtacggtac
     aggtgaaaaa ccgattgatg tgaagattgg gctttccctt gatggtggca tgagccctgc
     cgactggcgt cacgacgtca acccgctcgg caaatatatg ccaggtaaac cagcgtggta
     tatgtacgat agctgccaga gcaaacgcag cgacagtatc ggtgttctct gctctgcggt
     cttctggtca caaaacaatg gcctgcaact gcaaaatctg accatcgaaa actctctggg
     cgatggtaga tgcaggtaac catccgtgcg gtggcactgc gttactgatg gtgaccaggt
     acagattaac aacgtttctg aacgagaaac gtaaaatgat ataaatatca atatattaaa
     ttagattttg cataaaaaac agactacata atactgtaaa acacaacata tgcagtcact
     atgaatcaac tacttagatg gtattagtga cctgtaacag agcattagcg caaggtgatt
     tttgtcttct tgcgctaatt ttttgtcatc aaacctgtcg cactccagag aagcacaaag
     cctcgcaatc cagtgcaaag cttgggaaat agtacataat ggatttcctt acgcgaaata
     cgggcagaca tggcctgccc ggttattatt atttttgaca ccagaccaac tggtaatggt
     agcgaccggc gctcagctgg aattccgccg atactgacgg gctccaggag tcgtcgccac
     caatccccat atggaaaccg tcgatattca gccatgtgcc ttcttccgcg tgcagcagat
     ggcgatggct ggtttccatc agttgctgtt gactgtagcg gctgatgttg aactggaagt
     cgccgcgcca ctggtgtggg ccataattca attcgcgcgt cccgcagcgc agaccgtttt
     cgctcgggaa gacgtacggg gtatacatgt ctgacaatgg cagatcccag cggtcaaaac
     aggcggcagt aaggcggtcg ggatagtttt cttgcggccc taatccgagc cagtttaccc
     gctctgctac ctgcgccagc tggcagttca ggccaatccg cgccggatgc ggtgtatcgc
     tcgccacttc aacatcaacg gtaatcgcca tttgaccact accatcaatc cggtaggttt
     tccggctgat aaataaggtt ttcccctgat gctgccacgc gtgagcggtc gtaatcagca
     ccgcatcagc aagtgtatct gccgtgcact gcaacaacgc tgcttcggcc tggtaatggc
     ccgccgcctt ccagcgttcg acccaggcgt tagggtcaat gcgggtcgct tcacttacgc
     caatgtcgtt atccagcggt gcacgggtga actgatcgcg cagcggcgtc agcagttgtt
     ttttatcgcc aatccacatc tgtgaaagaa agcctgactg gcggttaaat tgccaacgct
     tattacccag ctcgatgcaa aaatccattt cgctggtggt cagatgcggg atggcgtggg
     acgcggcggg gagcgtcaca ctgaggtttt ccgccagacg ccactgctgc caggcgctga
     tgtgcccggc ttctgaccat gcggtcgcgt tcggttgcac tacgcgtact gtgagccaga
     gttgcccggc gctctccggc tgcggtagtt caggcagttc aatcaactgt ttaccttgtg
     gagcgacatc cagaggcact tcaccgcttg ccagcggctt accatccagc gccaccatcc
     agtgcaggag ctcgttatcg ctatgacgga acaggtattc gctggtcact tcgatggttt
     gcccggataa acggaactgg aaaaactgct gctggtgttt tgcttccgtc agcgctggat
     gcggcgtgcg gtcggcaaag accagaccgt tcatacagaa ctggcgatcg ttcggcgtat
     cgccaaaatc accgccgtaa gccgaccacg ggttgccgtt ttcatcatat ttaatcagcg
     actgatccac ccagtcccag acgaagccgc cctgtaaacg gggatactga cgaaacgcct
     gccagtattt agcgaaaccg ccaagactgt tacccatcgc gtgggcgtat tcgcaaagga
     tcagcgggcg cgtctctcca ggtagcgaaa gccatttttt gatggaccat ttcggcacag
     ccgggaaggg ctggtcttca tccacgcgcg cgtacatcgg gcaaataata tcggtggccg
     tggtgtcggc tccgccgcct tcatactgca ccgggcggga aggatcgaca gatttgatcc
     agcgatacag cgcgtcgtga ttagcgccgt ggcctgattc attccccagc gaccagatga
     tcacactcgg gtgattacga tcgcgctgca ccattcgcgt tacgcgttcg ctcatcgccg
     gtagccagcg cggatcatcg gtcagacgat tcattggcac catgccgtgg gtttcaatat
     tggcttcatc caccacatac aggccgtagc ggtcgcacag cgtgtaccac agcggatggt
     tcggataatg cgaacagcgc acggcgttaa agttgttctg cttcatcagc aggatatcct
     gcaccatcgt ctgctcatcc atgacctgac catgcagagg atgatgctcg tgacggttaa
     cgcctcgaat cagcaacggc ttgccgttca gcagcagcag accattttca atccgcacct
     cgcggaaacc gacatcgcag gcttctgctt caatcagcgt gccgtcggcg gtgtgcagtt
     caaccaccgc acgatagaga ttcgggattt cggcgctcca cagtttcggg ttttcgacgt
     tcagacgtag tgtgacgcga tcggcataac caccacgctc atcgataatt tcaccgccga
     aaggcgcggt gccgctggcg acctgcgttt caccctgcca taaagaaact gttacccgta
     ggtagtcacg caactcgccg cacatctgaa cttcagcctc cagtacagcg cggctgaaat
     catcattaaa gcgagtggca acatggaaat cgctgatttg tgtagtcggt ttatgcagca
     acgagacgtc acggaaaatg ccgctcatcc gccacatatc ctgatcttcc agataactgc
     cgtcactcca acgcagcacc atcaccgcga ggcggttttc tccggcgcgt aaaaatgcgc
     tcaggtcaaa ttcagacggc aaacgactgt cctggccgta accgacccag cgcccgttgc
     accacagatg aaacgccgag ttaacgccat caaaaataat tcgcgtctgg ccttcctgta
     gccagctttc atcaacatta aatgtgagcg agtaacaacc cgtcggattc tccgtgggaa
     caaacggcgg attgaccgta atgggatagg ttacgttggt gtagatgggc gcatcgtaac
     cgtgcatctg ccagtttgag gggacgacga cagtatcggc ctcaggaaga tcgcactcca
     gccagctttc cggcaccgct tctggtgccg gaaaccaggc aaagcgccat tcgccattca
     ggctgcgcaa ctgttgggaa gggcgatcgg tgcgggcctc ttcgctatta cgccagctgg
     cgaaaggggg atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac
     gacgttgtaa aacgacggcc agtgaatccg taatcatggt catagctgtt tcctgtgtga
     taaagaaagt taaaatgccg ccagcggaac tggcggctgt gggattaact gcgcgtcgcc
     gctttcatcg gttgtgggga tccccgggcg agctcgaatt agatcctaat ctagattagg
     taaatttatg gttggttatg aaatgctggc agagacccag cgagacctga ccgcagaaca
     ggcagcagag cgtttgcgcg cagtcagcga tatccatttt cgcgaatccg gagtgtaaga
     aatgagtctg aaagaaaaaa cacaatctct gtttgccaac gcatttggct accctgccac
     tcacaccatt caggcgcctg gccgcgtgaa tttgattggt gaacacaccg actacaacga
     cggtttcgtt ctgccctgcg cgattgatta tcaaaccgtg atcagttgtg caccacgcga
     tgaccgtaaa gttcgcgtga tggcagccga ttatgaaaat cagctcgacg agttttccct
     cgatgcgccc attgtcgcac atgaaaacta tcaatgggct aactacgttc gtggcgtggt
     gaaacatctg caactgcgta acaacagctt cggcggcgtg gacatggtga tcagcggcaa
     tgtgccgcag ggtgccgggt taagttcttc cgcttcactg gaagtcgcgg tcggaaccgt
     attgcagcag ctttatcatc tgccgctgga cggcgcacaa atcgcgctta acggtcagga
     agcagaaaac cagtttgtag gctgtaactg cgggatcatg gatcagctaa tttccgcgct
     cggcaagaaa gatcatgcct tgctgatcga ttgccgctca ctggggacca aagcagtttc
     catgcccaaa ggtgtggctg tcgtcatcat caacagtaac ttcaaacgta ccctggttgg
     cagcgaatac aacacccgtc gtgaacagtg cgaaaccggt gcgcgtttct tccagcagcc
     agccctgcgt gatgtcacca ttgaagagtt caacgctgtt gcgcatgaac tggacccgat
     cgtggcaaaa cgcgtgcgtc atatactgac tgaaaacgcc cgcaccgttg aagctgccag
     cgcgctggag caaggcgacc tgaaacgtat gggcgagttg atggcggagt ctcatgcctc
     tatgcgcgat gatttcgaaa tcaccgtgcc gcaaattgac actctggtag aaatcgtcaa
     agctgtgatt ggcgacaaag gtggcgtacg catgaccggc ggcggatttg gcggctgtat
     cgtcgcgctg atcccggaag agctggtgcc tgccgtacag caagctgtcg ctgaacaata
     tgaagcaaaa acaggtatta aagagacttt ttacgtttgt aaaccatcac aaggagcagg
     acagtgctga acgaaactcc cgcactggca cccgatggtc agccgtaccg actgttctgc
     ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca tgcagctccc ggagacggtc
     acagcttgtc tgtaagcgga tgccgggagc agacaagccc gtcagggcgc gtcagcgggt
     gttggcgggt gtcggggcgc agccatgacc cagtcacgta gcgatagcgg agtgtatact
     ggcttaacta tgcggcatca gagcagattg tactgagagt gcaccatatg cggtgtgaaa
     taccgcacag atgcgtaagg agaaaatacc gcatcaggcg ctcttccgct tcctcgctca
     ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg
     taatacggtt atccacagaa tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc
     agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc gtttttccat aggctccgcc
     cccctgacga gcatcacaaa aatcgacgct caagtcagag gtggcgaaac ccgacaggac
     tataaagata ccaggcgttt ccccctggaa gctccctcgt gcgctctcct gttccgaccc
     tgccgcttac cggatacctg tccgcctttc tcccttcggg aagcgtggcg ctttctcata
     gctcacgctg taggtatctc agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc
     acgaaccccc cgttcagccc gaccgctgcg ccttatccgg taactatcgt cttgagtcca
     acccggtaag acacgactta tcgccactgg cagcagccac tggtaacagg attagcagag
     cgaggtatgt aggcggtgct acagagttct tgaagtggtg gcctaactac ggctacacta
     gaaggacagt atttggtatc tgcgctctgc tgaagccagt taccttcgga aaaagagttg
     gtagctcttg atccggcaaa caaaccaccg ctggtagcgg tggttttttt gtttgcaagc
     agcagattac gcgcagaaaa aaaggatctc aagaagatcc tttgatcttt tctacggggt
     ctgacgctca gtggaacgaa aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa
     ggatcttcac ctagatcctt ttaaattaaa aatgaagttt taaatcaatc taaagtatat
     atgagtaaac ttggtctgac agttaccaat gcttaatcag tgaggcacct atctcagcga
     tctgtctatt tcgttcatcc atagttgcct gactccccgt cgtgtagata actacgatac
     gggagggctt accatctggc cccagtgctg caatgatacc gcgagaccca cgctcaccgg
     ctccagattt atcagcaata aaccagccag ccggaagggc cgagcgcaga agtggtcctg
     caactttatc cgcctccatc cagtctatta attgttgccg ggaagctaga gtaagtagtt
     cgccagttaa tagtttgcgc aacgttgttg ccattgctgc aggcatcgtg gtgtcacgct
     cgtcgtttgg tatggcttca ttcagctccg gttcccaacg atcaaggcga gttacatgat
     cccccatgtt gtgcaaaaaa gcggttagct ccttcggtcc tccgatcgtt gtcagaagta
     agttggccgc agtgttatca ctcatggtta tggcagcact gcataattct cttactgtca
     tgccatccgt aagatgcttt tctgtgactg gtgagtactc aaccaagtca ttctgagaat
     agtgtatgcg gcgaccgagt tgctcttgcc cggcgtcaac acgggataat accgcgccac
     atagcagaac tttaaaagtg ctcatcattg gaaaacgttc ttcggggcga aaactctcaa
     ggatcttacc gctgttgaga tccagttcga tgtaacccac tcgtgcaccc aactgatctt
     cagcatcttt tactttcacc agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg
     caaaaaaggg aataagggcg acacggaaat gttgaatact catactcttc ctttttcaat
     attattgaag catttatcag ggttattgtc tcatgagcgg atacatattt gaatgtattt
     agaaaaataa acaaataggg gttccgcgca catttccccg aaaagtgcca cctgacgtct
     aagaaaccat tattatcatg acattaacct ataaaaatag gcgtatcacg aggccctttc
     gtcttcaaga attgggtacc
//