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ID   PGEM11ZFM  preliminary; circular DNA; SYN; 3223 BP.
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AC   X65314; IG0148;
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DT   27-JAN-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEM-11Zf- - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-11Zf-, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-3223
RC   pGEM-11Zf-
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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CC   The sequence is the strand that
CC   is secreted by cells harboring pGEM-3Zf(+) plasmids.
CC   NM (pGEM-11Zf-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli JM109)
CC   CP ()
CC   FN (cloning)(transcription)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC13)(pGEM)
CC   BR (pGEM-11Zf+)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC13 400bp, f1 ori
FT                   2. pGEM-1 2865bp, ori/amp/lacZ/T7/Sp6
FT                   -> pGEM-11Zf- 3223bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    19..85
FT                   /note="MCS SfiI-SacI-EcoRI-SalI-XhoI-BamHI-ApaI-
FT                   XbaI-NotI-NsiI-HindIII"
FT   promoter        complement(88..110)
FT                   /note="PRO bacteriophage Sp6"
FT   misc_feature    93..93
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_feature    complement(118..347)
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     128..144
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    132..132
FT                   /note="E. coli lacZ start codon"
FT   promoter        complement(152..168)
FT                   /note="PRO E. coli lac operator"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1289..2149)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1842..1842
FT                   /note="SIT unique ScaI"
FT   misc_binding    1961..1961
FT                   /note="SIT unique XmnI"
FT   misc_binding    2284..2284
FT                   /note="SIT unique AatII"
FT   misc_binding    2533..2533
FT                   /note="SIT unique NdeI"
FT   rep_origin      2588..3043
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2715..2715
FT                   /note="SIT unique NaeI"
FT   misc_feature    complement(3044..3204)
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     3164..3180
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(3207..3223,1..6)
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 3223 BP; 783 A; 811 C; 809 G; 820 T; 0 other;
     gggcgaattg gccaagtcgg ccgagctcga attcgtcgac ctcgagggat ccgggccctc
     tagatgcggc cgcatgcata agcttgagta ttctatagtg tcacctaaat agcttggcgt
     aatcatggtc atagctgttt cctgtgtgaa attgttatcc gctcacaatt ccacacaaca
     tacgagccgg aagcataaag tgtaaagcct ggggtgccta atgagtgagc taactcacat
     taattgcgtt gcgctcactg cccgctttcc agtcgggaaa cctgtcgtgc cagctgcatt
     aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttcgataggc
     tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     gtagttcgcc agttaatagt ttgcgcaacg ttgttggcat tgctacaggc atcgtggtgt
     cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     gagaataccg cgcccggcga ccgagttgct cttgcccggc gtcaatacgg gataatagtg
     tatgacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     tcaggcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     cgatttagag ctttacggca cctcgaccgc aaaaaacttg atttgggtga tggttcacgt
     agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     atatttaacg cgaattttaa caaaatatta acgtttacaa tttccattcg ccattcaggc
     tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc cagctggcga
     aagggggatg tgctgcaagg cgattaagtt gggtaacgcc agggttttcc cagtcacgac
     gttgtaaaac gacggccagt gaattgtaat acgactcact ata
//