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ID PGEM13ZFP preliminary; circular DNA; SYN; 3181 BP.
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AC X65315; IG0149;
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DT 23-MAY-1989 (Rel. 4, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pGEM-13Zf+ - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pGEM-13Zf+, revised by [2]
RA ;
RT ;
RL Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [2]
RP 1-3181
RC pGEM-13Zf+
RA Klock C.;
RT ;
RL Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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CC The sequence is the complement of the strand that
CC is secreted by cells harboring pGEM-3Zf(+) plasmids.
CC NM (pGEM-13Zf+)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (non-coding)
CC TY (phagemid)
CC SP (Promega)
CC HO (E.coli JM109)
CC CP ()
CC FN (cloning)(transcription)(sequencing)(mutagenesis)
CC SE (color blue/white)
CC PA (pUC13)(pGEM)
CC BR (pGEM-13Zf-)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pUC13 400bp, f1 ori
FT 2. pGEM-1 2865bp, ori/amp/lacZ/T7/Sp6
FT -> pGEM-13Zf+ 3181bp"
FT misc_feature 1..1
FT /note="bacteriophage T7 transcription initiation
FT site"
FT misc_binding 11..44
FT /note="MCS NotI-SfiI-HindIII"
FT misc_feature 52..52
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT promoter complement(47..69)
FT /note="PRO bacteriophage Sp6"
FT CDS complement(76..323)
FT /note="GEN E. coli lac operon"
FT misc_feature complement(110..126)
FT /note="PRO E. coli lac operator"
FT primer_bind 86..102
FT /note="PRI bacteriophage M13 reverse"
FT misc_feature 93..93
FT /note="E. coli lacZ start codon"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1247..2107)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1800..1800
FT /note="SIT unique ScaI"
FT misc_binding 1919..1919
FT /note="SIT unique XmnI"
FT rep_origin complement(2546..3001)
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_binding 2873..2873
FT /note="SIT unique NaeI"
FT misc_feature complement(3002..3162)
FT /note="GEN E. coli lac operon"
FT primer_bind 3122..3188
FT /note="PRI bacteriophage M13 forward"
FT promoter join(3165..3181,1..6)
FT /note="PRO bacteriophage T7"
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SQ Sequence 3181 BP; 816 A; 783 C; 814 G; 768 T; 0 other;
gggcgaattg gcggccgctc cctggccgac ttggcccaag cttgagtatt ctatagtgtc
acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
tggcgttttt cgataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttggcattg
ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
actcaaccaa gtcattctga gaataccgcg cccggcgacc gagttgctct tgcccggcgt
caatacggga taatagtgta tgacatagca gaactttaaa agtgctcatc attggaaaac
gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta atattttgtt aaaattcgcg
ttaaatattt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
ggcccactac gtgaaccatc acccaaatca agttttttgc ggtcgaggtg ccgtaaagct
ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
a
//