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ID   PGEM13ZFP  preliminary; circular DNA; SYN; 3181 BP.
XX
AC   X65315; IG0149;
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DT   23-MAY-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEM-13Zf+ - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-13Zf+, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-3181
RC   pGEM-13Zf+
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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CC   The sequence is the complement of the strand that
CC   is secreted by cells harboring pGEM-3Zf(+) plasmids.
CC   NM (pGEM-13Zf+)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli JM109)
CC   CP ()
CC   FN (cloning)(transcription)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC13)(pGEM)
CC   BR (pGEM-13Zf-)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC13 400bp, f1 ori
FT                   2. pGEM-1 2865bp, ori/amp/lacZ/T7/Sp6
FT                   -> pGEM-13Zf+ 3181bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    11..44
FT                   /note="MCS NotI-SfiI-HindIII"
FT   misc_feature    52..52
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   promoter        complement(47..69)
FT                   /note="PRO bacteriophage Sp6"
FT   CDS             complement(76..323)
FT                   /note="GEN E. coli lac operon"
FT   misc_feature    complement(110..126)
FT                   /note="PRO E. coli lac operator"
FT   primer_bind     86..102
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    93..93
FT                   /note="E. coli lacZ start codon"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1247..2107)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1800..1800
FT                   /note="SIT unique ScaI"
FT   misc_binding    1919..1919
FT                   /note="SIT unique XmnI"
FT   rep_origin      complement(2546..3001)
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2873..2873
FT                   /note="SIT unique NaeI"
FT   misc_feature    complement(3002..3162)
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     3122..3188
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(3165..3181,1..6)
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 3181 BP; 816 A; 783 C; 814 G; 768 T; 0 other;
     gggcgaattg gcggccgctc cctggccgac ttggcccaag cttgagtatt ctatagtgtc
     acctaaatag cttggcgtaa tcatggtcat agctgtttcc tgtgtgaaat tgttatccgc
     tcacaattcc acacaacata cgagccggaa gcataaagtg taaagcctgg ggtgcctaat
     gagtgagcta actcacatta attgcgttgc gctcactgcc cgctttccag tcgggaaacc
     tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg
     ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag
     cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg gataacgcag
     gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc
     tggcgttttt cgataggctc cgcccccctg acgagcatca caaaaatcga cgctcaagtc
     agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct ggaagctccc
     tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc tttctccctt
     cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg gtgtaggtcg
     ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc tgcgccttat
     ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca ctggcagcag
     ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag ttcttgaagt
     ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct ctgctgaagc
     cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc accgctggta
     gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag
     atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca cgttaaggga
     ttttggtcat gagattatca aaaaggatct tcacctagat ccttttaaat taaaaatgaa
     gttttaaatc aatctaaagt atatatgagt aaacttggtc tgacagttac caatgcttaa
     tcagtgaggc acctatctca gcgatctgtc tatttcgttc atccatagtt gcctgactcc
     ccgtcgtgta gataactacg atacgggagg gcttaccatc tggccccagt gctgcaatga
     taccgcgaga cccacgctca ccggctccag atttatcagc aataaaccag ccagccggaa
     gggccgagcg cagaagtggt cctgcaactt tatccgcctc catccagtct attaattgtt
     gccgggaagc tagagtaagt agttcgccag ttaatagttt gcgcaacgtt gttggcattg
     ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc ttcattcagc tccggttccc
     aacgatcaag gcgagttaca tgatccccca tgttgtgcaa aaaagcggtt agctccttcg
     gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt atcactcatg gttatggcag
     cactgcataa ttctcttact gtcatgccat ccgtaagatg cttttctgtg actggtgagt
     actcaaccaa gtcattctga gaataccgcg cccggcgacc gagttgctct tgcccggcgt
     caatacggga taatagtgta tgacatagca gaactttaaa agtgctcatc attggaaaac
     gttcttcggg gcgaaaactc tcaaggatct taccgctgtt gagatccagt tcgatgtaac
     ccactcgtgc acccaactga tcttcagcat cttttacttt caccagcgtt tctgggtgag
     caaaaacagg aaggcaaaat gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa
     tactcatact cttccttttt caatattatt gaagcattta tcagggttat tgtctcatga
     gcggatacat atttgaatgt atttagaaaa ataaacaaat aggggttccg cgcacatttc
     cccgaaaagt gccacctgac gtctaagaaa ccattattat catgacatta acctataaaa
     ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgaaatt gtaaacgtta atattttgtt aaaattcgcg
     ttaaatattt gttaaatcag ctcatttttt aaccaatagg ccgaaatcgg caaaatccct
     tataaatcaa aagaatagac cgagataggg ttgagtgttg ttccagtttg gaacaagagt
     ccactattaa agaacgtgga ctccaacgtc aaagggcgaa aaaccgtcta tcagggcgat
     ggcccactac gtgaaccatc acccaaatca agttttttgc ggtcgaggtg ccgtaaagct
     ctaaatcgga accctaaagg gagcccccga tttagagctt gacggggaaa gccggcgaac
     gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg ctagggcgct ggcaagtgta
     gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta atgcgccgct acagggcgcg
     tccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc
     tattacgcca gctggcgaaa gggggatgtg ctgcaaggcg attaagttgg gtaacgccag
     ggttttccca gtcacgacgt tgtaaaacga cggccagtga attgtaatac gactcactat
     a
//