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ID   PGEM2      preliminary; circular DNA; SYN; 2869 BP.
XX
AC   X65301; IG0046;
XX
DT   06-AUG-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEM-2 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-2, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2869
RC   pGEM-2
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [3]
RC   pGEM-2 from pUC12
RA   Lewis K.;
RT   ;
RL   Unpublished (1987).
RL   Lewis K., Promega.
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CC   old Promega vector.
CC   NM (pGEM-2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE (color blue/white)
CC   PA (pUC12)
CC   BR (pGEM-1)(pGEM-3)(pGEM-4)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC12 2680bp, ori/amp/lacZ
FT                   2. T7, promoter
FT                   3. Sp6, promoter
FT                   -> pGEM-2 2869bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_feature    10..60
FT                   /note="MCS EcoRI-SmaI-BamHI-SalI-PstI-HindIII"
FT   misc_feature    70..70
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        65..87
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    98..156
FT                   /note="GEN E. coli lac operon"
FT   CDS             1098..1958
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        join(2852..2869,1..6)
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 2869 BP; 711 A; 742 C; 727 G; 689 T; 0 other;
     gaatacacgg aattcgagct cgcccgggga tcctctagag tcgacctgca gcccaagctt
     ccggtctccc tatagtgagt cgtattaatt tcgataagcc agctgcatta atgaatcggc
     caacgcgcgg ggagaggcgg tttgcgtatt gggcgctctt ccgcttcctc gctcactgac
     tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata
     cggttatcca cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa
     aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct
     gacgagcatc acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa
     agataccagg cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg
     cttaccggat acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca
     cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa
     ccccccgttc agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg
     gtaagacacg acttatcgcc actggcagca gccactggta acaggattag cagagcgagg
     tatgtaggcg gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg
     acagtatttg gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc
     tcttgatccg gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag
     attacgcgca gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac
     gctcagtgga acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc
     ttcacctaga tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag
     taaacttggt ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt
     ctatttcgtt catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag
     ggcttaccat ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca
     gatttatcag caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact
     ttatccgcct ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca
     gttaatagtt tgcgcaacgt tgttgccatt gctacaggca tcgtggtgtc acgctcgtcg
     tttggtatgg cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc
     atgttgtgca aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg
     gccgcagtgt tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca
     tccgtaagat gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt
     atgcggcgac cgagttgctc ttgcccggcg tcaatacggg ataataccgc gccacatagc
     agaactttaa aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc
     ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca
     tcttttactt tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa
     aagggaataa gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat
     tgaagcattt atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa
     aataaacaaa taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa
     accattatta tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctc
     gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga gacggtcaca
     gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc agcgggtgtt
     ggcgggtgtc ggggctggct taactatgcg gcatcagagc agattgtact gagagtgcac
     catatcgacg ctctccctta tgcgactcct gcattaggaa gcagcccagt agtaggttga
     ggccgttgag caccgccgcc gcaaggaatg gtgcatgcaa ggagatggcg cccaacagtc
     ccccggccac ggggcctgcc accataccca cgccgaaaca agcgctcatg agcccgaagt
     ggcgagcccg atcttcccca tcggtgatgt cggcgatata ggcgccagca accgcacctg
     tggcgccggt gatgccggcc acgatgcgtc cggcgtagag gatctggcta gcgatgaccc
     tgctgattgg ttcgctgacc atttccgggg tgcggaacgg cgttaccaga aactcagaag
     gttcgtccaa ccaaaccgac tctgacggca gtttacgaga gagatgatag ggtctgcttc
     agtaagccag atgctacaca attaggcttg tacatattgt cgttagaacg cggctacaat
     taatacataa ccttatgtat catacacata cgatttaggt gacactata
//