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ID   PGEM3ZFM   preliminary; circular DNA; SYN; 3199 BP.
XX
AC   X65307; IG0049;
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DT   02-JUL-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEM-3Zf- - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-3Zf-, revised by [3]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-3199
RC   pGEM-3Z from pGEM-3
RC   pGEM-3Zf-, pGEM-3Zf+ from pGEM-3Z
RA   Solomon L.R., Massom L.R., Jarrett H.W.;
RT   "Enzymatic syntheses of DNA-silicas using DNA polymerase";
RL   Anal. Biochem. 203:58-69(1992).
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RN   [3]
RP   1-3199
RC   pGEM-3Zf-
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [4]
RC   pGEM-3Zf- from pGEM-3Z
RA   Lewis K.;
RT   ;
RL   Unpublished (1987).
RL   Lewis K., Promega.
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CC   The sequence is the strand that
CC   is secreted by cells harboring pGEM-3Zf(-) plasmids.
CC   NM (pGEM-3Zf-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (coding)
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli JM109)
CC   CP ()
CC   FN (cloning)(transcription)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC18)(pGEM-3Z)
CC   BR (pGEM-3Zf+)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3Z 2743bp
FT                   2. pUC18 450bp, f1 ori
FT                   -> pGEM-3Zf- 3199bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    5..61
FT                   /note="MCS EcoRI-SacI-KpnI-AvaI-SmaI-BamHI-XbaI-
FT                   SalI-AccI-HincII-PstI-SphI-HindIII"
FT   misc_feature    69..69
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   promoter        complement(64..86)
FT                   /note="PRO bacteriophage Sp6"
FT   misc_feature    complement(94..323)
FT                   /note="GEN E. coli lac operon"
FT   misc_feature    108..108
FT                   /note="E. coli lacZ start codon"
FT   primer_bind     104..120
FT                   /note="PRI bacteriophage M13 reverse"
FT   promoter        complement(128..144)
FT                   /note="PRO E. coli lac operator"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1265..2125)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1818..1818
FT                   /note="SIT unique ScaI"
FT   misc_binding    1937..1937
FT                   /note="SIT unique XmnI"
FT   misc_binding    2260..2260
FT                   /note="SIT unique AatII"
FT   misc_binding    2509..2509
FT                   /note="SIT unique NdeI"
FT   rep_origin      2564..3019
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2691..2691
FT                   /note="SIT unique NaeI"
FT   misc_feature    complement(3020..3180)
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     3140..3156
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(3183..3199,1..6)
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 3199 BP; 779 A; 803 C; 801 G; 816 T; 0 other;
     gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
     tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
     tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
     aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
     ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
     gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
     tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
     aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcg ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     gcaacgttgt tggcattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     tttctgtgac tggtgagtac tcaaccaagt cattctgaga ataccgcgcc cggcgaccga
     gttgctcttg cccggcgtca atacgggata atagtgtatg acatagcaga actttaaaag
     tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
     atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
     cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
     gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     aaataccgca cagatgcgta aggagaaaat accgcatcag gcgacgcgcc ctgtagcggc
     gcattaagcg cggcgggtgt ggtggttacg cgcagcgtga ccgctacact tgccagcgcc
     ctagcgcccg ctcctttcgc tttcttccct tcctttctcg ccacgttcgc cggctttccc
     cgtcaagctc taaatcgggg gctcccttta gggttccgat ttagagcttt acggcacctc
     gaccgcaaaa aacttgattt gggtgatggt tcacgtagtg ggccatcgcc ctgatagacg
     gtttttcgcc ctttgacgtt ggagtccacg ttctttaata gtggactctt gttccaaact
     ggaacaacac tcaaccctat ctcggtctat tcttttgatt tataagggat tttgccgatt
     tcggcctatt ggttaaaaaa tgagctgatt taacaaatat ttaacgcgaa ttttaacaaa
     atattaacgt ttacaatttc cattcgccat tcaggctgcg caactgttgg gaagggcgat
     cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
     taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
     tgtaatacga ctcactata
//