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ID PGEM3ZFP preliminary; circular DNA; SYN; 3199 BP.
XX
AC X65306; IG0050;
XX
DT 02-JUL-1987 (Rel. 2, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pGEM-3Zf+ - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pGEM-3Zf+, revised by [3]
RA ;
RT ;
RL Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [2]
RP 1-3199
RC pGEM-3Z from pGEM-3
RC pGEM-3Zf-, pGEM-3Zf+ from pGEM-3Z
RA Solomon L.R., Massom L.R., Jarrett H.W.;
RT "Enzymatic syntheses of DNA-silicas using DNA polymerase";
RL Anal. Biochem. 203:58-69(1992).
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RN [3]
RP 1-3199
RC pGEM-3Zf+
RA Klock C.;
RT ;
RL Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [4]
RC pGEM-3Zf+ from pGEM-3Z
RA Lewis K.;
RT ;
RL Unpublished (1987).
RL Lewis K., Promega.
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CC The sequence is the complement of the strand that
CC is secreted by cells harboring pGEM-3Zf(+) plasmids.
CC NM (pGEM-3Zf+)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST (non-coding)
CC TY (phagemid)
CC SP (Promega)
CC HO (E.coli JM109)
CC CP ()
CC FN (cloning)(transcription)(sequencing)(mutagenesis)
CC SE (color blue/white)
CC PA (pUC18)(pGEM-3Z)
CC BR (pGEM-3Zf-)
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEM-3Z 2743bp
FT 2. pUC18 450bp, f1 ori
FT -> pGEM-3Zf+ 3199bp"
FT misc_feature 1..1
FT /note="bacteriophage T7 transcription initiation
FT site"
FT misc_binding 5..61
FT /note="MCS EcoRI-SacI-KpnI-AvaI-SmaI-BamHI-XbaI-
FT SalI-AccI-HincII-PstI-SphI-HindIII"
FT misc_feature 69..69
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT promoter complement(64..86)
FT /note="PRO bacteriophage Sp6"
FT misc_feature complement(94..323)
FT /note="GEN E. coli lac operon"
FT misc_feature 108..108
FT /note="E. coli lacZ start codon"
FT primer_bind 104..120
FT /note="PRI bacteriophage M13 reverse"
FT promoter complement(128..144)
FT /note="PRO E. coli lac operator"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1265..2125)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1818..1818
FT /note="SIT unique ScaI"
FT misc_binding 1937..1937
FT /note="SIT unique XmnI"
FT misc_binding 2260..2260
FT /note="SIT unique AatII"
FT misc_binding 2509..2509
FT /note="SIT unique NdeI"
FT rep_origin complement(2564..3019)
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_binding 2691..2691
FT /note="SIT unique NaeI"
FT misc_feature complement(3020..3180)
FT /note="GEN E. coli lac operon"
FT primer_bind 3140..3156
FT /note="PRI bacteriophage M13 forward"
FT promoter join(3183..3199,1..6)
FT /note="PRO bacteriophage T7"
XX
SQ Sequence 3199 BP; 823 A; 785 C; 819 G; 772 T; 0 other;
gggcgaattc gagctcggta cccggggatc ctctagagtc gacctgcagg catgcaagct
tgagtattct atagtgtcac ctaaatagct tggcgtaatc atggtcatag ctgtttcctg
tgtgaaattg ttatccgctc acaattccac acaacatacg agccggaagc ataaagtgta
aagcctgggg tgcctaatga gtgagctaac tcacattaat tgcgttgcgc tcactgcccg
ctttccagtc gggaaacctg tcgtgccagc tgcattaatg aatcggccaa cgcgcgggga
gaggcggttt gcgtattggg cgctcttccg cttcctcgct cactgactcg ctgcgctcgg
tcgttcggct gcggcgagcg gtatcagctc actcaaaggc ggtaatacgg ttatccacag
aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
gtaaaaaggc cgcgttgctg gcgtttttcg ataggctccg cccccctgac gagcatcaca
aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
gcaacgttgt tggcattgct acaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
tttctgtgac tggtgagtac tcaaccaagt cattctgaga ataccgcgcc cggcgaccga
gttgctcttg cccggcgtca atacgggata atagtgtatg acatagcaga actttaaaag
tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtctcgcg cgtttcggtg
atgacggtga aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag
cggatgccgg gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg
gctggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
aaataccgca cagatgcgta aggagaaaat accgcatcag gcgaaattgt aaacgttaat
attttgttaa aattcgcgtt aaatatttgt taaatcagct cattttttaa ccaataggcc
gaaatcggca aaatccctta taaatcaaaa gaatagaccg agatagggtt gagtgttgtt
ccagtttgga acaagagtcc actattaaag aacgtggact ccaacgtcaa agggcgaaaa
accgtctatc agggcgatgg cccactacgt gaaccatcac ccaaatcaag ttttttgcgg
tcgaggtgcc gtaaagctct aaatcggaac cctaaaggga gcccccgatt tagagcttga
cggggaaagc cggcgaacgt ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct
agggcgctgg caagtgtagc ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat
gcgccgctac agggcgcgtc cattcgccat tcaggctgcg caactgttgg gaagggcgat
cggtgcgggc ctcttcgcta ttacgccagc tggcgaaagg gggatgtgct gcaaggcgat
taagttgggt aacgccaggg ttttcccagt cacgacgttg taaaacgacg gccagtgaat
tgtaatacga ctcactata
//