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ID   PGEM4Z     preliminary; circular DNA; SYN; 2746 BP.
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AC   X65305; IG0052;
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DT   02-JUL-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pGEM-4Z - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-4Z, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2746
RC   pGEM-4Z
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pGEM-4Z from pUC18
RA   Lewis K.;
RT   ;
RL   Unpublished (1987).
RL   Lewis K., Promega.
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CC   NM (pGEM-4Z)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli JM109)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE (color blue/white)
CC   PA (pUC18)
CC   BR (pGEM-3Z)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-4 remove, 2871bp-
FT                   2. E. coli, lac operon
FT                   -> pGEM-4Z 2746bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    7..63
FT                   /note="MCS EcoRI-SacI-KpnI-AvaI-SmaI-BamHI-XbaI-
FT                   SalI-AccI-HincII-PstI-SphI-HindIII"
FT   misc_feature    70..70
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        complement(65..87)
FT                   /note="PRO bacteriophage T7"
FT   misc_feature    complement(96..325)
FT                   /note="GEN E. coli lac operon"
FT   misc_feature    110..110
FT                   /note="E. coli lacZ start codon"
FT   primer_bind     110..126
FT                   /note="PRI bacteriophage M13 reverse"
FT   promoter        complement(130..146)
FT                   /note="PRO E. coli lac operator"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1267..2127)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1820..1820
FT                   /note="SIT unique ScaI"
FT   misc_binding    1939..1939
FT                   /note="SIT unique XmnI"
FT   misc_binding    2144..2144
FT                   /note="SIT unique SspI"
FT   misc_binding    2262..2262
FT                   /note="SIT unique AatII"
FT   misc_binding    2511..2511
FT                   /note="SIT unique NdeI"
FT   misc_binding    2563..2563
FT                   /note="SIT unique NarI"
FT   misc_feature    complement(2563..2726)
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     2684..2700
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(2729..2746,1..6)
FT                   /note="PRO bacteriophage Sp6"
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SQ   Sequence 2746 BP; 683 A; 681 C; 703 G; 679 T; 0 other;
     gaatacgaat tcgagctcgg tacccgggga tcctctagag tcgacctgca ggcatgcaag
     cttgtctccc tatagtgagt cgtattagag cttggcgtaa tcatggtcat agctgtttcc
     tgtgtgaaat tgttatccgc tcacaattcc acacaacata cgagccggaa gcataaagtg
     taaagcctgg ggtgcctaat gagtgagcta actcacatta attgcgttgc gctcactgcc
     cgctttcgag tcgggaaacc tgtcgtgcca gctgcattaa tgaatcggcc aacgcgcggg
     gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg ctcactgact cgctgcgctc
     ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac
     agaatcaggg gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa
     ccgtaaaaag gccgcgttgc tggcgttttt cgataggctc cgcccccctg acgagcatca
     caaaaatcga cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc
     gtttccccct ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata
     cctgtccgcc tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta
     tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca
     gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga
     cttatcgcca ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg
     tgctacagag ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg
     tatctgcgct ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg
     caaacaaacc accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag
     aaaaaaagga tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa
     cgaaaactca cgttaaggga ttttggtcat gagattatca aaaaggatct tcacctagat
     ccttttaaat taaaaatgaa gttttaaatc aatctaaagt atatatgagt aaacttggtc
     tgacagttac caatgcttaa tcagtgaggc acctatctca gcgatctgtc tatttcgttc
     atccatagtt gcctgactcc ccgtcgtgta gataactacg atacgggagg gcttaccatc
     tggccccagt gctgcaatga taccgcgaga cccacgctca ccggctccag atttatcagc
     aataaaccag ccagccggaa gggccgagcg cagaagtggt cctgcaactt tatccgcctc
     catccagtct attaattgtt gccgggaagc tagagtaagt agttcgccag ttaatagttt
     gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca cgctcgtcgt ttggtatggc
     ttcattcagc tccggttccc aacgatcaag gcgagttaca tgatccccca tgttgtgcaa
     aaaagcggtt agctccttcg gtcctccgat cgttgtcaga agtaagttgg ccgcagtgtt
     atcactcatg gttatggcag cactgcataa ttctcttact gtcatgccat ccgtaagatg
     cttttctgtg actggtgagt actcaaccaa gtcattctga gaatagtgta tgcggcgacc
     gagttgctct tgcccggcgt caatacggga taataccgcg ccacatagca gaactttaaa
     agtgctcatc attggaaaac gttcttcggg gcgaaaactc tcaaggatct taccgctgtt
     gagatccagt tcgatgtaac ccactcgtgc acccaactga tcttcagcat cttttacttt
     caccagcgtt tctgggtgag caaaaacagg aaggcaaaat gccgcaaaaa agggaataag
     ggcgacacgg aaatgttgaa tactcatact cttccttttt caatattatt gaagcattta
     tcagggttat tgtctcatga gcggatacat atttgaatgt atttagaaaa ataaacaaat
     aggggttccg cgcacatttc cccgaaaagt gccacctgac gtctaagaaa ccattattat
     catgacatta acctataaaa ataggcgtat cacgaggccc tttcgtctcg cgcgtttcgg
     tgatgacggt gaaaacctct gacacatgca gctcccggag acggtcacag cttgtctgta
     agcggatgcc gggagcagac aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg
     gggctggctt aactatgcgg catcagagca gattgtactg agagtgcacc atatgcggtg
     tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgccatt cgccattcag
     gctgcgcaac tgttgggaag ggcgatcggt gcgggcctct tcgctattac gccagctggc
     gaaaggggga tgtgctgcaa ggcgattaag ttgggtaacg ccagggtttt cccagtcacg
     acgttgtaaa acgacggcca gtgaattgga tttaggtgac actata
//