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ID   PGEM7ZFP   preliminary; circular DNA; SYN; 3000 BP.
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AC   X65310; IG0054;
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DT   07-AUG-1987 (Rel. 2, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEM-7Zf+ - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-7Zf+, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-3000
RC   pGEM-7Zf+
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pGEM-7Zf+ from pGEM series
RA   Lewis K.;
RT   ;
RL   Unpublished (1987).
RL   Lewis K., Promega.
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CC   The sequence is the complement of the strand that
CC   is secreted by cells harboring pGEM-3Zf(+) plasmids.
CC   NM (pGEM-7Zf+)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST (non-coding)
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli JM109)
CC   CP ()
CC   FN (cloning)(transcription)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC)(pGEM)
CC   BR (pGEM-7Zf-)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3Z 2743bp
FT                   2. pUC18 250bp, f1 ori
FT                   -> pGEM-7Zf+ 3000bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    10..110
FT                   /note="MCS ApaI-AatII-SphI-XbaI-XhoI-EcoRI-KpnI-
FT                   SmaI-Csp45I-ClaI-HindIII-BamHI-SacI-BstXI-NsiI"
FT   misc_feature    123..123
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   promoter        complement(118..140)
FT                   /note="PRO bacteriophage Sp6"
FT   misc_feature    complement(148..377)
FT                   /note="GEN E. coli lac operon"
FT   promoter        complement(182..198)
FT                   /note="PRO E. coli lac operator"
FT   primer_bind     158..174
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    162..162
FT                   /note="E. coli lacZ start codon"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1319..2179)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1872..1872
FT                   /note="SIT unique ScaI"
FT   misc_binding    1991..1991
FT                   /note="SIT unique XmnI"
FT   rep_origin      complement(2365..2820)
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2492..2492
FT                   /note="SIT unique NaeI"
FT   misc_feature    complement(2821..2981)
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     2941..2957
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(2984..3000,1..6)
FT                   /note="PRO bacteriophage T7"
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SQ   Sequence 3000 BP; 774 A; 737 C; 759 G; 730 T; 0 other;
     gggcgaattg ggcccgacgt cgcatgctcc tctagactcg aggaattcgg taccccgggt
     tcgaaatcga taagcttgga tccggagagc tcccaacgcg ttggatgcat agcttgagta
     ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttcgataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttggcat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaataccg cgcccggcga ccgagttgct
     cttgcccggc gtcaatacgg gataatagtg tatgacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg tatgcggtgt gaaataccgc acagatgcgt
     aaggagaaaa taccgcatca ggcgaaattg taaacgttaa tattttgtta aaattcgcgt
     taaatatttg ttaaatcagc tcatttttta accaataggc cgaaatcggc aaaatccctt
     ataaatcaaa agaatagacc gagatagggt tgagtgttgt tccagtttgg aacaagagtc
     cactattaaa gaacgtggac tccaacgtca aagggcgaaa aaccgtctat cagggcgatg
     gcccactacg tgaaccatca cccaaatcaa gttttttgcg gtcgaggtgc cgtaaagctc
     taaatcggaa ccctaaaggg agcccccgat ttagagcttg acggggaaag ccggcgaacg
     tggcgagaaa ggaagggaag aaagcgaaag gagcgggcgc tagggcgctg gcaagtgtag
     cggtcacgct gcgcgtaacc accacacccg ccgcgcttaa tgcgccgcta cagggcgcgt
     ccattcgcca ttcaggctgc gcaactgttg ggaagggcga tcggtgcggg cctcttcgct
     attacgccag ctggcgaaag ggggatgtgc tgcaaggcga ttaagttggg taacgccagg
     gttttcccag tcacgacgtt gtaaaacgac ggccagtgaa ttgtaatacg actcactata
//