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ID PGEM9ZFM preliminary; circular DNA; SYN; 2925 BP.
XX
AC X65312; IG0146;
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DT 14-APR-1988 (Rel. 3, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pGEM-9Zf- - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pGEM-9Zf-, revised by [2]
RA ;
RT ;
RL Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [2]
RP 1-2925
RC pGEM-9Zf-
RA Klock C.;
RT ;
RL Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [3]
RC pGEM-9Zf- from pGEM series
RA Lewis K.;
RT ;
RL Unpublished (1988).
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CC The sequence is the strand that
CC is secreted by cells harboring pGEM-3Zf(+) plasmids.
CC NM (pGEM-9Zf-)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Promega)
CC HO (E.coli JM109)
CC CP ()
CC FN (cloning)(transcription)(sequencing)(mutagenesis)
CC SE (color blue/white)
CC PA (pUC)(pGEM)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEM-9Z
FT 2. pUC18, f1 ori
FT -> pGEM-7Zf- 2925bp"
FT misc_feature 1..1
FT /note="bacteriophage T7 transcription initiation
FT site"
FT misc_binding 5..54
FT /note="MCS NsiI-SpeI-HindIII-XbaI-EcoRI-SalI-SacI"
FT misc_feature 55..55
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT promoter complement(50..72)
FT /note="PRO bacteriophage Sp6"
FT misc_binding 82..82
FT /note="SIT unique SfiI"
FT misc_feature complement(93..322)
FT /note="GEN E. coli lac operon"
FT promoter complement(127..143)
FT /note="PRO E. coli lac operator"
FT primer_bind 103..119
FT /note="PRI bacteriophage M13 reverse"
FT misc_feature 107..107
FT /note="E. coli lacZ start codon"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1264..2124)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1817..1817
FT /note="SIT unique ScaI"
FT misc_binding 1936..1936
FT /note="SIT unique XmnI"
FT rep_origin 2256..2722
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_binding 2383..2383
FT /note="SIT unique NaeI"
FT misc_feature complement(2724..2881)
FT /note="GEN E. coli lac operon"
FT primer_bind 2844..2860
FT /note="PRI bacteriophage M13 forward"
FT promoter join(2909..2925,1..6)
FT /note="PRO bacteriophage T7"
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SQ Sequence 2925 BP; 714 A; 740 C; 711 G; 760 T; 0 other;
gaatatgcat cactagtaag ctttgctcta gactggaatt cgtcgacgag ctccctatag
tgagtcgtat tagaggccga cttggccaaa ttcgtaatca tggtcatagc tgtttcctgt
gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
aaaatattaa cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg
cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
gccaagctga cttggtcagc ggccgcagat ttaggtgaca ctata
//