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ID   PGEM9ZFM   preliminary; circular DNA; SYN; 2925 BP.
XX
AC   X65312; IG0146;
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DT   14-APR-1988 (Rel. 3, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEM-9Zf- - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-9Zf-, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-2925
RC   pGEM-9Zf-
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [3]
RC   pGEM-9Zf- from pGEM series
RA   Lewis K.;
RT   ;
RL   Unpublished (1988).
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CC   The sequence is the strand that
CC   is secreted by cells harboring pGEM-3Zf(+) plasmids.
CC   NM (pGEM-9Zf-)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli JM109)
CC   CP ()
CC   FN (cloning)(transcription)(sequencing)(mutagenesis)
CC   SE (color blue/white)
CC   PA (pUC)(pGEM)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-9Z
FT                   2. pUC18, f1 ori
FT                   -> pGEM-7Zf- 2925bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    5..54
FT                   /note="MCS NsiI-SpeI-HindIII-XbaI-EcoRI-SalI-SacI"
FT   misc_feature    55..55
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   promoter        complement(50..72)
FT                   /note="PRO bacteriophage Sp6"
FT   misc_binding    82..82
FT                   /note="SIT unique SfiI"
FT   misc_feature    complement(93..322)
FT                   /note="GEN E. coli lac operon"
FT   promoter        complement(127..143)
FT                   /note="PRO E. coli lac operator"
FT   primer_bind     103..119
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    107..107
FT                   /note="E. coli lacZ start codon"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1264..2124)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1817..1817
FT                   /note="SIT unique ScaI"
FT   misc_binding    1936..1936
FT                   /note="SIT unique XmnI"
FT   rep_origin      2256..2722
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2383..2383
FT                   /note="SIT unique NaeI"
FT   misc_feature    complement(2724..2881)
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     2844..2860
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(2909..2925,1..6)
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 2925 BP; 714 A; 740 C; 711 G; 760 T; 0 other;
     gaatatgcat cactagtaag ctttgctcta gactggaatt cgtcgacgag ctccctatag
     tgagtcgtat tagaggccga cttggccaaa ttcgtaatca tggtcatagc tgtttcctgt
     gtgaaattgt tatccgctca caattccaca caacatacga gccggaagca taaagtgtaa
     agcctggggt gcctaatgag tgagctaact cacattaatt gcgttgcgct cactgcccgc
     tttccagtcg ggaaacctgt cgtgccagct gcattaatga atcggccaac gcgcggggag
     aggcggtttg cgtattgggc gctcttccgc ttcctcgctc actgactcgc tgcgctcggt
     cgttcggctg cggcgagcgg tatcagctca ctcaaaggcg gtaatacggt tatccacaga
     atcaggggat aacgcaggaa agaacatgtg agcaaaaggc cagcaaaagg ccaggaaccg
     taaaaaggcc gcgttgctgg cgtttttcca taggctccgc ccccctgacg agcatcacaa
     aaatcgacgc tcaagtcaga ggtggcgaaa cccgacagga ctataaagat accaggcgtt
     tccccctgga agctccctcg tgcgctctcc tgttccgacc ctgccgctta ccggatacct
     gtccgccttt ctcccttcgg gaagcgtggc gctttctcat agctcacgct gtaggtatct
     cagttcggtg taggtcgttc gctccaagct gggctgtgtg cacgaacccc ccgttcagcc
     cgaccgctgc gccttatccg gtaactatcg tcttgagtcc aacccggtaa gacacgactt
     atcgccactg gcagcagcca ctggtaacag gattagcaga gcgaggtatg taggcggtgc
     tacagagttc ttgaagtggt ggcctaacta cggctacact agaaggacag tatttggtat
     ctgcgctctg ctgaagccag ttaccttcgg aaaaagagtt ggtagctctt gatccggcaa
     acaaaccacc gctggtagcg gtggtttttt tgtttgcaag cagcagatta cgcgcagaaa
     aaaaggatct caagaagatc ctttgatctt ttctacgggg tctgacgctc agtggaacga
     aaactcacgt taagggattt tggtcatgag attatcaaaa aggatcttca cctagatcct
     tttaaattaa aaatgaagtt ttaaatcaat ctaaagtata tatgagtaaa cttggtctga
     cagttaccaa tgcttaatca gtgaggcacc tatctcagcg atctgtctat ttcgttcatc
     catagttgcc tgactccccg tcgtgtagat aactacgata cgggagggct taccatctgg
     ccccagtgct gcaatgatac cgcgagaccc acgctcaccg gctccagatt tatcagcaat
     aaaccagcca gccggaaggg ccgagcgcag aagtggtcct gcaactttat ccgcctccat
     ccagtctatt aattgttgcc gggaagctag agtaagtagt tcgccagtta atagtttgcg
     caacgttgtt gccattgcta caggcatcgt ggtgtcacgc tcgtcgtttg gtatggcttc
     attcagctcc ggttcccaac gatcaaggcg agttacatga tcccccatgt tgtgcaaaaa
     agcggttagc tccttcggtc ctccgatcgt tgtcagaagt aagttggccg cagtgttatc
     actcatggtt atggcagcac tgcataattc tcttactgtc atgccatccg taagatgctt
     ttctgtgact ggtgagtact caaccaagtc attctgagaa tagtgtatgc ggcgaccgag
     ttgctcttgc ccggcgtcaa tacgggataa taccgcgcca catagcagaa ctttaaaagt
     gctcatcatt ggaaaacgtt cttcggggcg aaaactctca aggatcttac cgctgttgag
     atccagttcg atgtaaccca ctcgtgcacc caactgatct tcagcatctt ttactttcac
     cagcgtttct gggtgagcaa aaacaggaag gcaaaatgcc gcaaaaaagg gaataagggc
     gacacggaaa tgttgaatac tcatactctt cctttttcaa tattattgaa gcatttatca
     gggttattgt ctcatgagcg gatacatatt tgaatgtatt tagaaaaata aacaaatagg
     ggttccgcgc acatttcccc gaaaagtgcc acctgacgcg ccctgtagcg gcgcattaag
     cgcggcgggt gtggtggtta cgcgcagcgt gaccgctaca cttgccagcg ccctagcgcc
     cgctcctttc gctttcttcc cttcctttct cgccacgttc gccggctttc cccgtcaagc
     tctaaatcgg ggcatccctt tagggttccg atttagtgct ttacggcacc tcgaccccaa
     aaaacttgat tagggtgatg gttcacgtag tgggccatcg ccctgataga cggtttttcg
     ccctttgacg ttggagtcca cgttctttaa tagtggactc ttgttccaaa ctggaacaac
     actcaaccct atctcggtct attcttttga tttataaggg attttgccga tttcggccta
     ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca aaatattaac
     aaaatattaa cgtttacaat ttcccattcg ccattcaggc tgcgcaactg ttgggaaggg
     cgatcggtgc gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg
     cgattaagtt gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt
     gccaagctga cttggtcagc ggccgcagat ttaggtgaca ctata
//