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ID   PGEMEX1    preliminary; circular DNA; SYN; 3995 BP.
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AC   X65317; IG0154;
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DT   18-DEC-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEMEX-1 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEMEX-1, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-3995
RC   pGEMEX-1
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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CC   NM (pGEMEX-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli JM109DE3)
CC   CP ()
CC   FN (cloning)(transcription)(expression)(mutagenesis)(sequencing)
CC   SE (color blue/white)
CC   PA (pUC)
CC   BR (pGEMEX-2)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3Zf+ 3199bp
FT                   2. T7, gene 10
FT                   -> pGEMEX-1 3995bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    9..84
FT                   /note="MCS HindIII-NsiI-SphI-NotI-ApaI-BamHI-XhoI-
FT                   SalI-EcoRI-SacI-SfiI"
FT   misc_feature    89..89
FT                   /note="bacteriophage T3 transcription initiation
FT                   site"
FT   promoter        complement(84..106)
FT                   /note="PRO bacteriophage T3"
FT   CDS             complement(91..905)
FT                   /note="GEN bacteriophage T7 gene 10"
FT   misc_feature    905..905
FT                   /note="bacteriophage T7 gene 10 start codon"
FT   misc_feature    968..968
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        complement(963..985)
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    1008..1008
FT                   /note="SIT unique SmaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2007..2867)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2560..2560
FT                   /note="SIT unique ScaI"
FT   misc_binding    3002..3002
FT                   /note="SIT unique AatII"
FT   rep_origin      3306..3761
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    3433..3433
FT                   /note="SIT unique NaeI"
FT   terminator      3841..3931
FT                   /note="PRO bacteriophage T7"
FT   promoter        join(3979..3995,1..6)
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 3995 BP; 971 A; 1029 C; 988 G; 1007 T; 0 other;
     gaatactcaa gcttatgcat gcggccgcat ctagagggcc cggatccctc gaggtcgacg
     aattcgagct cggccgactt ggccttccct ttagtgaggg ttaataaact tggccaattc
     gatccggtac cagcaccacc agcggtgagg tgcggaactt ctacaacctc aaagcccata
     acgttgcgga tagaaccctt ctcagggtca atcagagcag cgtagtttgc tgcgttcggc
     atcagtgctg ccagaatcgc agagtagcta tctgggtcac agtagaacac acggtcagca
     gccggaacat agttcttggt cagagccgca cgagccttag tcagagccgc aataatctcc
     ttacccagcg caacttggtc ggtaagtgcg gccttgttct gagtggtctc aattacggta
     gcagtaccta agccctcgat gttctcatta tatttgcttt ccacgttaca cagaccggca
     atctcagcca gaaccgcacc atccgcagcc atcgccagag attcacccaa ctgagaggta
     tactcagagc gaacgtcgta gtggttcatc gcgtcctcaa tatcataaat cagaacgtca
     gccgtcagga gaccgtcaat ggtgattacc ttctcggtgt gtttgatgtc cttacgttta
     tcgtcgaggt tctcgcccgg agccagatac gctgcctgag tgcgacccag aacagggaac
     tgagcggatt taccgctgga gatggaacgt accatgtggc gagaagtggt cacggaggta
     cgagcgaacg cagtcaggac ttcaccgcca aataccttca agaacaacgc cagtttatct
     ccagcagcaa ctacaccttt accttggtta gtacccattt gctgtccacc agtcatgcta
     gccatatgta tatctccttc ttaaagttaa acaaaattat ttctagaggg aaaccgttgt
     ggtctcccta tagtgagtcg tattaatttc gcgggatcga gatctcccgg gctgcattaa
     tgaatcggcc aacgcgcggg gagaggcggt ttgcgtattg ggcgctcttc cgcttcctcg
     ctcactgact cgctgcgctc ggtcgttcgg ctgcggcgag cggtatcagc tcactcaaag
     gcggtaatac ggttatccac agaatcaggg gataacgcag gaaagaacat gtgagcaaaa
     ggccagcaaa aggccaggaa ccgtaaaaag gccgcgttgc tggcgttttt cgataggctc
     cgcccccctg acgagcatca caaaaatcga cgctcaagtc agaggtggcg aaacccgaca
     ggactataaa gataccaggc gtttccccct ggaagctccc tcgtgcgctc tcctgttccg
     accctgccgc ttaccggata cctgtccgcc tttctccctt cgggaagcgt ggcgctttct
     catagctcac gctgtaggta tctcagttcg gtgtaggtcg ttcgctccaa gctgggctgt
     gtgcacgaac cccccgttca gcccgaccgc tgcgccttat ccggtaacta tcgtcttgag
     tccaacccgg taagacacga cttatcgcca ctggcagcag ccactggtaa caggattagc
     agagcgaggt atgtaggcgg tgctacagag ttcttgaagt ggtggcctaa ctacggctac
     actagaagga cagtatttgg tatctgcgct ctgctgaagc cagttacctt cggaaaaaga
     gttggtagct cttgatccgg caaacaaacc accgctggta gcggtggttt ttttgtttgc
     aagcagcaga ttacgcgcag aaaaaaagga tctcaagaag atcctttgat cttttctacg
     gggtctgacg ctcagtggaa cgaaaactca cgttaaggga ttttggtcat gagattatca
     aaaaggatct tcacctagat ccttttaaat taaaaatgaa gttttaaatc aatctaaagt
     atatatgagt aaacttggtc tgacagttac caatgcttaa tcagtgaggc acctatctca
     gcgatctgtc tatttcgttc atccatagtt gcctgactcc ccgtcgtgta gataactacg
     atacgggagg gcttaccatc tggccccagt gctgcaatga taccgcgaga cccacgctca
     ccggctccag atttatcagc aataaaccag ccagccggaa gggccgagcg cagaagtggt
     cctgcaactt tatccgcctc catccagtct attaattgtt gccgggaagc tagagtaagt
     agttcgccag ttaatagttt gcgcaacgtt gttggcattg ctacaggcat cgtggtgtca
     cgctcgtcgt ttggtatggc ttcattcagc tccggttccc aacgatcaag gcgagttaca
     tgatccccca tgttgtgcaa aaaagcggtt agctccttcg gtcctccgat cgttgtcaga
     agtaagttgg ccgcagtgtt atcactcatg gttatggcag cactgcataa ttctcttact
     gtcatgccat ccgtaagatg cttttctgtg actggtgagt actcaaccaa gtcattctga
     gaataccgcg cccggcgacc gagttgctct tgcccggcgt caatacggga taatagtgta
     tgacatagca gaactttaaa agtgctcatc attggaaaac gttcttcggg gcgaaaactc
     tcaaggatct taccgctgtt gagatccagt tcgatgtaac ccactcgtgc acccaactga
     tcttcagcat cttttacttt caccagcgtt tctgggtgag caaaaacagg aaggcaaaat
     gccgcaaaaa agggaataag ggcgacacgg aaatgttgaa tactcatact cttccttttt
     caatattatt gaagcattta tcagggttat tgtctcatga gcggatacat atttgaatgt
     atttagaaaa ataaacaaat aggggttccg cgcacatttc cccgaaaagt gccacctgac
     gtctaagaaa ccattattat catgacatta acctataaaa ataggcgtat cacgaggccc
     tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg catcagagca gattgtactg
     agagtgcacc atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc
     aggcgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     atttagagct ttacggcacc tcgaccgcaa aaaacttgat ttgggtgatg gttcacgtag
     tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaat
     atttaacgcg aattttaaca aaatattaac gtttacaatt tccattcgcc attcaggctg
     cgcaactgtt gggaagggcg atcggtgcgg gcctcttcgc tattacgcca ggttatccgg
     atatagttcc tcctttcagc aaaaaacccc tcaagacccg tttagaggcc ccaaggggtt
     atgctagtta ttgctcagcg gtggcagcag ccaactcagc ttcctttcgg gctttgttag
     cagccggatc aagctgggat ttaggtgaca ctata
//