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ID   PGEMEX2    preliminary; circular DNA; SYN; 3997 BP.
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AC   X65318; IG0155;
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DT   18-DEC-1989 (Rel. 4, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEMEX-2 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEMEX-2, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-3997
RC   pGEMEX-2
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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CC   NM (pGEMEX-2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli JM109DE3)
CC   CP ()
CC   FN (cloning)(transcription)(expression)(mutagenesis)(sequencing)
CC   SE (color blue/white)
CC   PA (pUC)
CC   BR (pGEMEX-1)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3Zf+ 3199bp
FT                   2. T7, gene 10
FT                   -> pGEMEX-2 3997bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   misc_binding    9..85
FT                   /note="MCS HindIII-NsiI-SphI-NotI-ApaI-BamHI-XhoI-
FT                   SalI-EcoRI-SacI-SfiI"
FT   misc_feature    91..91
FT                   /note="bacteriophage T3 transcription initiation
FT                   site"
FT   promoter        complement(86..109)
FT                   /note="PRO bacteriophage T3"
FT   CDS             complement(93..907)
FT                   /note="GEN bacteriophage T7 gene 10"
FT   misc_feature    907..907
FT                   /note="bacteriophage T7 gene 10 start codon"
FT   misc_feature    970..970
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   promoter        complement(965..987)
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    1010..1010
FT                   /note="SIT unique SmaI"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(2009..2869)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    2562..2562
FT                   /note="SIT unique ScaI"
FT   misc_binding    3004..3004
FT                   /note="SIT unique AatII"
FT   rep_origin      3308..3763
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    3435..3435
FT                   /note="SIT unique NaeI"
FT   terminator      3843..3933
FT                   /note="PRO bacteriophage T7"
FT   promoter        join(3981..3997,1..6)
FT                   /note="PRO bacteriophage Sp6"
XX
SQ   Sequence 3997 BP; 970 A; 1031 C; 989 G; 1007 T; 0 other;
     gaatactcaa gcttatgcat gcggccgcgt ctagagggcc cggatccctc gaggtcgacg
     aattccgagc tcggccgact tggcccttcc ctttagtgag ggttaataaa cttggccaat
     tcgatccggt accagcacca ccagcggtga ggtgcggaac ttctacaacc tcaaagccca
     taacgttgcg gatagaaccc ttctcagggt caatcagagc agcgtagttt gctgcgttcg
     gcatcagtgc tgccagaatc gcagagtagc tatctgggtc acagtagaac acacggtcag
     cagccggaac atagttcttg gtcagagccg cacgagcctt agtcagagcc gcaataatct
     ccttacccag cgcaacttgg tcggtaagtg cggccttgtt ctgagtggtc tcaattacgg
     tagcagtacc taagccctcg atgttctcat tatatttgct ttccacgtta cacagaccgg
     caatctcagc cagaaccgca ccatccgcag ccatcgccag agattcaccc aactgagagg
     tatactcaga gcgaacgtcg tagtggttca tcgcgtcctc aatatcataa atcagaacgt
     cagccgtcag gagaccgtca atggtgatta ccttctcggt gtgtttgatg tccttacgtt
     tatcgtcgag gttctcgccc ggagccagat acgctgcctg agtgcgaccc agaacaggga
     actgagcgga tttaccgctg gagatggaac gtaccatgtg gcgagaagtg gtcacggagg
     tacgagcgaa cgcagtcagg acttcaccgc caaatacctt caagaacaac gccagtttat
     ctccagcagc aactacacct ttaccttggt tagtacccat ttgctgtcca ccagtcatgc
     tagccatatg tatatctcct tcttaaagtt aaacaaaatt atttctagag ggaaaccgtt
     gtggtctccc tatagtgagt cgtattaatt tcgcgggatc gagatctccc gggctgcatt
     aatgaatcgg ccaacgcgcg gggagaggcg gtttgcgtat tgggcgctct tccgcttcct
     cgctcactga ctcgctgcgc tcggtcgttc ggctgcggcg agcggtatca gctcactcaa
     aggcggtaat acggttatcc acagaatcag gggataacgc aggaaagaac atgtgagcaa
     aaggccagca aaaggccagg aaccgtaaaa aggccgcgtt gctggcgttt ttcgataggc
     tccgcccccc tgacgagcat cacaaaaatc gacgctcaag tcagaggtgg cgaaacccga
     caggactata aagataccag gcgtttcccc ctggaagctc cctcgtgcgc tctcctgttc
     cgaccctgcc gcttaccgga tacctgtccg cctttctccc ttcgggaagc gtggcgcttt
     ctcatagctc acgctgtagg tatctcagtt cggtgtaggt cgttcgctcc aagctgggct
     gtgtgcacga accccccgtt cagcccgacc gctgcgcctt atccggtaac tatcgtcttg
     agtccaaccc ggtaagacac gacttatcgc cactggcagc agccactggt aacaggatta
     gcagagcgag gtatgtaggc ggtgctacag agttcttgaa gtggtggcct aactacggct
     acactagaag gacagtattt ggtatctgcg ctctgctgaa gccagttacc ttcggaaaaa
     gagttggtag ctcttgatcc ggcaaacaaa ccaccgctgg tagcggtggt ttttttgttt
     gcaagcagca gattacgcgc agaaaaaaag gatctcaaga agatcctttg atcttttcta
     cggggtctga cgctcagtgg aacgaaaact cacgttaagg gattttggtc atgagattat
     caaaaaggat cttcacctag atccttttaa attaaaaatg aagttttaaa tcaatctaaa
     gtatatatga gtaaacttgg tctgacagtt accaatgctt aatcagtgag gcacctatct
     cagcgatctg tctatttcgt tcatccatag ttgcctgact ccccgtcgtg tagataacta
     cgatacggga gggcttacca tctggcccca gtgctgcaat gataccgcga gacccacgct
     caccggctcc agatttatca gcaataaacc agccagccgg aagggccgag cgcagaagtg
     gtcctgcaac tttatccgcc tccatccagt ctattaattg ttgccgggaa gctagagtaa
     gtagttcgcc agttaatagt ttgcgcaacg ttgttggcat tgctacaggc atcgtggtgt
     cacgctcgtc gtttggtatg gcttcattca gctccggttc ccaacgatca aggcgagtta
     catgatcccc catgttgtgc aaaaaagcgg ttagctcctt cggtcctccg atcgttgtca
     gaagtaagtt ggccgcagtg ttatcactca tggttatggc agcactgcat aattctctta
     ctgtcatgcc atccgtaaga tgcttttctg tgactggtga gtactcaacc aagtcattct
     gagaataccg cgcccggcga ccgagttgct cttgcccggc gtcaatacgg gataatagtg
     tatgacatag cagaacttta aaagtgctca tcattggaaa acgttcttcg gggcgaaaac
     tctcaaggat cttaccgctg ttgagatcca gttcgatgta acccactcgt gcacccaact
     gatcttcagc atcttttact ttcaccagcg tttctgggtg agcaaaaaca ggaaggcaaa
     atgccgcaaa aaagggaata agggcgacac ggaaatgttg aatactcata ctcttccttt
     ttcaatatta ttgaagcatt tatcagggtt attgtctcat gagcggatac atatttgaat
     gtatttagaa aaataaacaa ataggggttc cgcgcacatt tccccgaaaa gtgccacctg
     acgtctaaga aaccattatt atcatgacat taacctataa aaataggcgt atcacgaggc
     cctttcgtct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     cagcgggtgt tggcgggtgt cggggctggc ttaactatgc ggcatcagag cagattgtac
     tgagagtgca ccatatgcgg tgtgaaatac cgcacagatg cgtaaggaga aaataccgca
     tcaggcgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt tacgcgcagc
     gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt cccttccttt
     ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc tttagggttc
     cgatttagag ctttacggca cctcgaccgc aaaaaacttg atttgggtga tggttcacgt
     agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc cacgttcttt
     aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt ctattctttt
     gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct gatttaacaa
     atatttaacg cgaattttaa caaaatatta acgtttacaa tttccattcg ccattcaggc
     tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc gctattacgc caggttatcc
     ggatatagtt cctcctttca gcaaaaaacc cctcaagacc cgtttagagg ccccaagggg
     ttatgctagt tattgctcag cggtggcagc agccaactca gcttcctttc gggctttgtt
     agcagccgga tcaagctggg atttaggtga cactata
//