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ID PGEMLUC preliminary; circular DNA; SYN; 4933 BP.
XX
AC X65316; IG0156;
XX
DT 01-JAN-1991 (Rel. 6, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE E. coli phagemid vector pGEM-luc - complete.
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KW cloning vector.
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OS Cloning vector
OC Artificial sequences; Cloning vehicles.
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RN [1]
RC pGEM-luc, revised by [2]
RA ;
RT ;
RL Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 53711-5399, USA.
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RN [2]
RP 1-4933
RC pGEM-luc
RA Klock C.;
RT ;
RL Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL Madison, WI 5371-5399, USA.
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CC NM (pGEM-luc)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Promega)
CC HO (E.coli)
CC CP ()
CC FN (cloning)(transcription)(luciferase production)
CC SE (color)
CC PA (pGEM-11Zf-)
CC BR ()
CC OF ()
CC OR ()
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FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEM-3Zf+ remove SalI-HindIII 18bp 39..57,
FT \ 3181bp
FT 2. firefly SalI-HindIII 1755bp, luciferase gene
FT \ 36..1791
FT -> pGEM-luc 4933bp"
FT misc_feature 1..1
FT /note="bacteriophage T7 transcription initiation
FT site"
FT misc_binding 9..47
FT /note="MCS SfiI-SacI-EcoRI-SalI-XhoI-StuI"
FT misc_feature 57..1756
FT /note="firefly luciferase cDNA"
FT CDS 105..1754
FT /note="GEN firefly luciferase gene (luxA)"
FT misc_feature 1762..1795
FT /note="MCS BamHI-ApaI-XbaI-NotI-NsiI-HindIII"
FT misc_feature 1803..1803
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT promoter 1798..1820
FT /note="PRO bacteriophage Sp6"
FT misc_feature 1828..2057
FT /note="GEN E. coli lac operon"
FT primer_bind 1834..1854
FT /note="PRI bacteriophage M13 reverse"
FT misc_feature 1842..1842
FT /note="E. coli lacZ start codon"
FT promoter 1853..1880
FT /note="PRO E. coli lacZ gene operator"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 3002..3859
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT rep_origin 4298..4751
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_feature 4754..4912
FT /note="GEN E. coli lac operon"
FT primer_bind 4874..4890
FT /note="PRI bacteriophage M13 forward"
FT promoter join(4917..4933,1..6)
FT /note="PRO bacteriophage T7"
XX
SQ Sequence 4933 BP; 1234 A; 1217 C; 1165 G; 1317 T; 0 other;
gggcgaattg gccaagtcgg ccgagctcga attcgtcgac ctcgaggcct cggaggatta
caatagctaa gaatttcgtc atcgctgaat acagttacat tttacaattt ggactttccg
cccttcttgg cctttatgag gatctctctg atttttcttg cgtcgagttt tccggtaaga
cctttcggta cttcgtccac aaacacaact cctccgcgca actttttcgc ggttgttact
tgactggcca cgtaatccac gatctctttt tccgtcatcg tctttccgtg ctccaaaaca
acaacggcgg cgggaagttc accggcgtca tcgtcgggaa gacctgccac gcccgcgtcg
aagatgttgg ggtgttgtaa caatatcgat tccaattcag cgggggccac ctgatatcct
ttgtatttaa ttaaagactt caagcggtca actatgaaga agtgttcgtc ttcgtcccag
taagctatgt ctccagaatg tagccatcca tccttgtcaa tcaaggcgtt ggtcgcttcc
ggattgttta cataaccgga cataatcata ggtcctctga cacataattc gcctctctga
ttaacgccca gcgttttccc ggtatccaga tccacaacct tcgcttcaaa aaatggaaca
actttaccga ccgcgcccgg tttatcatcc ccctcgggtg taatcagaat agctgatgta
gtctcagtga gcccatatcc ttgtcgtatc cctggaagat ggaagcgttt tgcaaccgct
tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa tttcgtgtaa attagataaa
tcgtatttgt caatcagagt gcttttggcg aagaatgaaa atagggttgg tactagcaac
gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca gctcttcttc aaatctatac
attaagacga ctcgaaatcc acatatcaaa tatccgagtg tagtaaacat tccaaaaccg
tgatggaatg gaacaacact taaaatcgca gtatccggaa tgatttgatt gccaaaaata
ggatctctgg catgcgagaa tctgacgcag gcagttctat gcggaagggc cacaccctta
ggtaacccag tagatccaga ggaattcatt atcagtgcaa ttgttttgtc acgatcaaag
gactctggta caaaatcgta ttcattaaaa ccgggaggta gatgagatgt gacgaacgtg
tacatcgact gaaatccctg gtaatccgtt ttagaatcca tgataataat tttctggatt
attggtaatt ttttttgcac gttcaaaatt ttttgcaacc cctttttgga aacaaacact
acggtaggct gcgaaatgtt catactgttg agcaattcac gttcattata aatgtcgttc
gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca ccggcataaa gaattgaaga
gagttttcac tgcatacgac gattctgtga tttgtattca gcccatatcg tttcatagct
tctgccaacc gaacggacat ttcgaagtat tccgcgtacg tgatgttcac ctcgatatgt
gcatctgtaa aagcaattgt tccaggaacc agggcgtatc tcttcatagc cttatgcagt
tgctctccag cggttccatc ctctagagga tagaatggcg ccgggccttt ctttatgttt
ttggcgtctt ccatttggat ccgggccctc tagatgcggc cgcatgcata agcttgagta
ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
aggccgcgtt gctggcgttt ttcgataggc tccgcccccc tgacgagcat cacaaaaatc
gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
ttgttggcat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
tgactggtga gtactcaacc aagtcattct gagaataccg cgcccggcga ccgagttgct
cttgcccggc gtcaatacgg gataatagtg tatgacatag cagaacttta aaagtgctca
tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
cgcacagatg cgtaaggaga aaataccgca tcaggcgacg cgccctgtag cggcgcatta
agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
gctctaaatc gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc
aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
tattggttaa aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta
acgtttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
acgactcact ata
//