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ID   PGEMLUC    preliminary; circular DNA; SYN; 4933 BP.
XX
AC   X65316; IG0156;
XX
DT   01-JAN-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGEM-luc - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGEM-luc, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [2]
RP   1-4933
RC   pGEM-luc
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 5371-5399, USA.
XX
CC   NM (pGEM-luc)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)(luciferase production)
CC   SE (color)
CC   PA (pGEM-11Zf-)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-3Zf+ remove SalI-HindIII 18bp 39..57,
FT                   \ 3181bp
FT                   2. firefly SalI-HindIII 1755bp, luciferase gene
FT                   \ 36..1791
FT                   -> pGEM-luc 4933bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    9..47
FT                   /note="MCS SfiI-SacI-EcoRI-SalI-XhoI-StuI"
FT   misc_feature    57..1756
FT                   /note="firefly luciferase cDNA"
FT   CDS             105..1754
FT                   /note="GEN firefly luciferase gene (luxA)"
FT   misc_feature    1762..1795
FT                   /note="MCS BamHI-ApaI-XbaI-NotI-NsiI-HindIII"
FT   misc_feature    1803..1803
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   promoter        1798..1820
FT                   /note="PRO bacteriophage Sp6"
FT   misc_feature    1828..2057
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     1834..1854
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    1842..1842
FT                   /note="E. coli lacZ start codon"
FT   promoter        1853..1880
FT                   /note="PRO E. coli lacZ gene operator"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             3002..3859
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      4298..4751
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_feature    4754..4912
FT                   /note="GEN E. coli lac operon"
FT   primer_bind     4874..4890
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        join(4917..4933,1..6)
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 4933 BP; 1234 A; 1217 C; 1165 G; 1317 T; 0 other;
     gggcgaattg gccaagtcgg ccgagctcga attcgtcgac ctcgaggcct cggaggatta
     caatagctaa gaatttcgtc atcgctgaat acagttacat tttacaattt ggactttccg
     cccttcttgg cctttatgag gatctctctg atttttcttg cgtcgagttt tccggtaaga
     cctttcggta cttcgtccac aaacacaact cctccgcgca actttttcgc ggttgttact
     tgactggcca cgtaatccac gatctctttt tccgtcatcg tctttccgtg ctccaaaaca
     acaacggcgg cgggaagttc accggcgtca tcgtcgggaa gacctgccac gcccgcgtcg
     aagatgttgg ggtgttgtaa caatatcgat tccaattcag cgggggccac ctgatatcct
     ttgtatttaa ttaaagactt caagcggtca actatgaaga agtgttcgtc ttcgtcccag
     taagctatgt ctccagaatg tagccatcca tccttgtcaa tcaaggcgtt ggtcgcttcc
     ggattgttta cataaccgga cataatcata ggtcctctga cacataattc gcctctctga
     ttaacgccca gcgttttccc ggtatccaga tccacaacct tcgcttcaaa aaatggaaca
     actttaccga ccgcgcccgg tttatcatcc ccctcgggtg taatcagaat agctgatgta
     gtctcagtga gcccatatcc ttgtcgtatc cctggaagat ggaagcgttt tgcaaccgct
     tccccgactt ctttcgaaag aggtgcgccc ccagaagcaa tttcgtgtaa attagataaa
     tcgtatttgt caatcagagt gcttttggcg aagaatgaaa atagggttgg tactagcaac
     gcactttgaa ttttgtaatc ctgaagggat cgtaaaaaca gctcttcttc aaatctatac
     attaagacga ctcgaaatcc acatatcaaa tatccgagtg tagtaaacat tccaaaaccg
     tgatggaatg gaacaacact taaaatcgca gtatccggaa tgatttgatt gccaaaaata
     ggatctctgg catgcgagaa tctgacgcag gcagttctat gcggaagggc cacaccctta
     ggtaacccag tagatccaga ggaattcatt atcagtgcaa ttgttttgtc acgatcaaag
     gactctggta caaaatcgta ttcattaaaa ccgggaggta gatgagatgt gacgaacgtg
     tacatcgact gaaatccctg gtaatccgtt ttagaatcca tgataataat tttctggatt
     attggtaatt ttttttgcac gttcaaaatt ttttgcaacc cctttttgga aacaaacact
     acggtaggct gcgaaatgtt catactgttg agcaattcac gttcattata aatgtcgttc
     gcgggcgcaa ctgcaactcc gataaataac gcgcccaaca ccggcataaa gaattgaaga
     gagttttcac tgcatacgac gattctgtga tttgtattca gcccatatcg tttcatagct
     tctgccaacc gaacggacat ttcgaagtat tccgcgtacg tgatgttcac ctcgatatgt
     gcatctgtaa aagcaattgt tccaggaacc agggcgtatc tcttcatagc cttatgcagt
     tgctctccag cggttccatc ctctagagga tagaatggcg ccgggccttt ctttatgttt
     ttggcgtctt ccatttggat ccgggccctc tagatgcggc cgcatgcata agcttgagta
     ttctatagtg tcacctaaat agcttggcgt aatcatggtc atagctgttt cctgtgtgaa
     attgttatcc gctcacaatt ccacacaaca tacgagccgg aagcataaag tgtaaagcct
     ggggtgccta atgagtgagc taactcacat taattgcgtt gcgctcactg cccgctttcc
     agtcgggaaa cctgtcgtgc cagctgcatt aatgaatcgg ccaacgcgcg gggagaggcg
     gtttgcgtat tgggcgctct tccgcttcct cgctcactga ctcgctgcgc tcggtcgttc
     ggctgcggcg agcggtatca gctcactcaa aggcggtaat acggttatcc acagaatcag
     gggataacgc aggaaagaac atgtgagcaa aaggccagca aaaggccagg aaccgtaaaa
     aggccgcgtt gctggcgttt ttcgataggc tccgcccccc tgacgagcat cacaaaaatc
     gacgctcaag tcagaggtgg cgaaacccga caggactata aagataccag gcgtttcccc
     ctggaagctc cctcgtgcgc tctcctgttc cgaccctgcc gcttaccgga tacctgtccg
     cctttctccc ttcgggaagc gtggcgcttt ctcatagctc acgctgtagg tatctcagtt
     cggtgtaggt cgttcgctcc aagctgggct gtgtgcacga accccccgtt cagcccgacc
     gctgcgcctt atccggtaac tatcgtcttg agtccaaccc ggtaagacac gacttatcgc
     cactggcagc agccactggt aacaggatta gcagagcgag gtatgtaggc ggtgctacag
     agttcttgaa gtggtggcct aactacggct acactagaag gacagtattt ggtatctgcg
     ctctgctgaa gccagttacc ttcggaaaaa gagttggtag ctcttgatcc ggcaaacaaa
     ccaccgctgg tagcggtggt ttttttgttt gcaagcagca gattacgcgc agaaaaaaag
     gatctcaaga agatcctttg atcttttcta cggggtctga cgctcagtgg aacgaaaact
     cacgttaagg gattttggtc atgagattat caaaaaggat cttcacctag atccttttaa
     attaaaaatg aagttttaaa tcaatctaaa gtatatatga gtaaacttgg tctgacagtt
     accaatgctt aatcagtgag gcacctatct cagcgatctg tctatttcgt tcatccatag
     ttgcctgact ccccgtcgtg tagataacta cgatacggga gggcttacca tctggcccca
     gtgctgcaat gataccgcga gacccacgct caccggctcc agatttatca gcaataaacc
     agccagccgg aagggccgag cgcagaagtg gtcctgcaac tttatccgcc tccatccagt
     ctattaattg ttgccgggaa gctagagtaa gtagttcgcc agttaatagt ttgcgcaacg
     ttgttggcat tgctacaggc atcgtggtgt cacgctcgtc gtttggtatg gcttcattca
     gctccggttc ccaacgatca aggcgagtta catgatcccc catgttgtgc aaaaaagcgg
     ttagctcctt cggtcctccg atcgttgtca gaagtaagtt ggccgcagtg ttatcactca
     tggttatggc agcactgcat aattctctta ctgtcatgcc atccgtaaga tgcttttctg
     tgactggtga gtactcaacc aagtcattct gagaataccg cgcccggcga ccgagttgct
     cttgcccggc gtcaatacgg gataatagtg tatgacatag cagaacttta aaagtgctca
     tcattggaaa acgttcttcg gggcgaaaac tctcaaggat cttaccgctg ttgagatcca
     gttcgatgta acccactcgt gcacccaact gatcttcagc atcttttact ttcaccagcg
     tttctgggtg agcaaaaaca ggaaggcaaa atgccgcaaa aaagggaata agggcgacac
     ggaaatgttg aatactcata ctcttccttt ttcaatatta ttgaagcatt tatcagggtt
     attgtctcat gagcggatac atatttgaat gtatttagaa aaataaacaa ataggggttc
     cgcgcacatt tccccgaaaa gtgccacctg acgtctaaga aaccattatt atcatgacat
     taacctataa aaataggcgt atcacgaggc cctttcgtct cgcgcgtttc ggtgatgacg
     gtgaaaacct ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg
     ccgggagcag acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggctggc
     ttaactatgc ggcatcagag cagattgtac tgagagtgca ccatatgcgg tgtgaaatac
     cgcacagatg cgtaaggaga aaataccgca tcaggcgacg cgccctgtag cggcgcatta
     agcgcggcgg gtgtggtggt tacgcgcagc gtgaccgcta cacttgccag cgccctagcg
     cccgctcctt tcgctttctt cccttccttt ctcgccacgt tcgccggctt tccccgtcaa
     gctctaaatc gggggctccc tttagggttc cgatttagag ctttacggca cctcgaccgc
     aaaaaacttg atttgggtga tggttcacgt agtgggccat cgccctgata gacggttttt
     cgccctttga cgttggagtc cacgttcttt aatagtggac tcttgttcca aactggaaca
     acactcaacc ctatctcggt ctattctttt gatttataag ggattttgcc gatttcggcc
     tattggttaa aaaatgagct gatttaacaa atatttaacg cgaattttaa caaaatatta
     acgtttacaa tttccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc
     gggcctcttc gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt
     gggtaacgcc agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat
     acgactcact ata
//