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ID PGEMT preliminary; circular DNA; SYN; 3003 BP.
XX
AC IG1165;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli phagemid vector pGEM-T - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC pGEM-T from pGEM-5Zf+
RA Lewis K.;
RT ;
RL Unpublished (1987).
RL Lewis K., Promega.
XX
CC pGEM-T has dT, which improves efficiency of ligation of PCR product.
CC NM (pGEM-T)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (phagemid)
CC SP (Promega)
CC HO (E.coli)
CC CP ()
CC FN (cloning)(transcription)
CC SE (color blue/white)
CC PA (pGEM-5Zf+)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEM-5Zf+ 3003bp
FT -> pGEM-T 3003bp"
FT misc_feature 1..1
FT /note="bacteriophage T7 transcription initiation
FT site"
FT misc_binding 10..46
FT /note="MCS ApaI-AatII-SphI-NcoI-SacII"
FT misc_feature 47..54
FT /note="3' T overhangs"
FT misc_binding 55..113
FT /note="MCS SpeI-NotI-PstI-SalI-NdeI-SacI-BstXI-NsiI"
FT misc_feature 126..126
FT /note="bacteriophage Sp6 transcription initiation
FT site"
FT promoter complement(127..143)
FT /note="PRO bacteriophage Sp6"
FT CDS complement(151..180)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT promoter complement(151..201)
FT /note="PRO E. coli lac operator"
FT primer_bind 161..177
FT /note="PRI bacteriophage M13 reverse"
FT misc_feature 165..165
FT /note="E. coli lacZ start codon"
FT rep_origin complement(0..0)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(1322..2182)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 1875..1875
FT /note="SIT unique ScaI"
FT misc_binding 1994..1994
FT /note="SIT unique XmnI"
FT rep_origin complement(2368..2823)
FT /note="ORI bacteriophage f1 intergenic region"
FT misc_binding 2695..2695
FT /note="SIT unique NaeI"
FT promoter complement(2824..2984)
FT /note="GEN E. coli beta-galactosidase gene (lacZ)
FT alpha peptide"
FT primer_bind 2944..2960
FT /note="PRI bacteriophage M13 forward"
FT promoter 2987..3003
FT /note="PRO bacteriophage T7"
XX
SQ Sequence 3003 BP; 770 A; 743 C; 764 G; 726 T; 0 other;
gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tatcactagt
gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga
gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
aaaaggccgc gttgctggcg tttttcgata ggctccgccc ccctgacgag catcacaaaa
atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
acgttgttgg cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
ctgtgactgg tgagtactca accaagtcat tctgagaata ccgcgcccgg cgaccgagtt
gctcttgccc ggcgtcaata cgggataata gtgtatgaca tagcagaact ttaaaagtgc
tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
ttccgcgcac atttccccga aaagtgccac ctgtatgcgg tgtgaaatac cgcacagatg
cgtaaggaga aaataccgca tcaggcgaaa ttgtaaacgt taatattttg ttaaaattcg
cgttaaatat ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag
ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
ata
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