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ID   PGEMT      preliminary; circular DNA; SYN; 3003 BP.
XX
AC   IG1165;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pGEM-T - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGEM-T from pGEM-5Zf+
RA   Lewis K.;
RT   ;
RL   Unpublished (1987).
RL   Lewis K., Promega.
XX
CC   pGEM-T has dT, which improves efficiency of ligation of PCR product.
CC   NM (pGEM-T)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(transcription)
CC   SE (color blue/white)
CC   PA (pGEM-5Zf+)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEM-5Zf+ 3003bp
FT                   -> pGEM-T 3003bp"
FT   misc_feature    1..1
FT                   /note="bacteriophage T7 transcription initiation
FT                   site"
FT   misc_binding    10..46
FT                   /note="MCS ApaI-AatII-SphI-NcoI-SacII"
FT   misc_feature    47..54
FT                   /note="3' T overhangs"
FT   misc_binding    55..113
FT                   /note="MCS SpeI-NotI-PstI-SalI-NdeI-SacI-BstXI-NsiI"
FT   misc_feature    126..126
FT                   /note="bacteriophage Sp6 transcription initiation
FT                   site"
FT   promoter        complement(127..143)
FT                   /note="PRO bacteriophage Sp6"
FT   CDS             complement(151..180)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   promoter        complement(151..201)
FT                   /note="PRO E. coli lac operator"
FT   primer_bind     161..177
FT                   /note="PRI bacteriophage M13 reverse"
FT   misc_feature    165..165
FT                   /note="E. coli lacZ start codon"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(1322..2182)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    1875..1875
FT                   /note="SIT unique ScaI"
FT   misc_binding    1994..1994
FT                   /note="SIT unique XmnI"
FT   rep_origin      complement(2368..2823)
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   misc_binding    2695..2695
FT                   /note="SIT unique NaeI"
FT   promoter        complement(2824..2984)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)
FT                   alpha peptide"
FT   primer_bind     2944..2960
FT                   /note="PRI bacteriophage M13 forward"
FT   promoter        2987..3003
FT                   /note="PRO bacteriophage T7"
XX
SQ   Sequence 3003 BP; 770 A; 743 C; 764 G; 726 T; 0 other;
     gggcgaattg ggcccgacgt cgcatgctcc cggccgccat ggccgcggga tatcactagt
     gcggccgcct gcaggtcgac catatgggag agctcccaac gcgttggatg catagcttga
     gtattctata gtgtcaccta aatagcttgg cgtaatcatg gtcatagctg tttcctgtgt
     gaaattgtta tccgctcaca attccacaca acatacgagc cggaagcata aagtgtaaag
     cctggggtgc ctaatgagtg agctaactca cattaattgc gttgcgctca ctgcccgctt
     tccagtcggg aaacctgtcg tgccagctgc attaatgaat cggccaacgc gcggggagag
     gcggtttgcg tattgggcgc tcttccgctt cctcgctcac tgactcgctg cgctcggtcg
     ttcggctgcg gcgagcggta tcagctcact caaaggcggt aatacggtta tccacagaat
     caggggataa cgcaggaaag aacatgtgag caaaaggcca gcaaaaggcc aggaaccgta
     aaaaggccgc gttgctggcg tttttcgata ggctccgccc ccctgacgag catcacaaaa
     atcgacgctc aagtcagagg tggcgaaacc cgacaggact ataaagatac caggcgtttc
     cccctggaag ctccctcgtg cgctctcctg ttccgaccct gccgcttacc ggatacctgt
     ccgcctttct cccttcggga agcgtggcgc tttctcatag ctcacgctgt aggtatctca
     gttcggtgta ggtcgttcgc tccaagctgg gctgtgtgca cgaacccccc gttcagcccg
     accgctgcgc cttatccggt aactatcgtc ttgagtccaa cccggtaaga cacgacttat
     cgccactggc agcagccact ggtaacagga ttagcagagc gaggtatgta ggcggtgcta
     cagagttctt gaagtggtgg cctaactacg gctacactag aaggacagta tttggtatct
     gcgctctgct gaagccagtt accttcggaa aaagagttgg tagctcttga tccggcaaac
     aaaccaccgc tggtagcggt ggtttttttg tttgcaagca gcagattacg cgcagaaaaa
     aaggatctca agaagatcct ttgatctttt ctacggggtc tgacgctcag tggaacgaaa
     actcacgtta agggattttg gtcatgagat tatcaaaaag gatcttcacc tagatccttt
     taaattaaaa atgaagtttt aaatcaatct aaagtatata tgagtaaact tggtctgaca
     gttaccaatg cttaatcagt gaggcaccta tctcagcgat ctgtctattt cgttcatcca
     tagttgcctg actccccgtc gtgtagataa ctacgatacg ggagggctta ccatctggcc
     ccagtgctgc aatgataccg cgagacccac gctcaccggc tccagattta tcagcaataa
     accagccagc cggaagggcc gagcgcagaa gtggtcctgc aactttatcc gcctccatcc
     agtctattaa ttgttgccgg gaagctagag taagtagttc gccagttaat agtttgcgca
     acgttgttgg cattgctaca ggcatcgtgg tgtcacgctc gtcgtttggt atggcttcat
     tcagctccgg ttcccaacga tcaaggcgag ttacatgatc ccccatgttg tgcaaaaaag
     cggttagctc cttcggtcct ccgatcgttg tcagaagtaa gttggccgca gtgttatcac
     tcatggttat ggcagcactg cataattctc ttactgtcat gccatccgta agatgctttt
     ctgtgactgg tgagtactca accaagtcat tctgagaata ccgcgcccgg cgaccgagtt
     gctcttgccc ggcgtcaata cgggataata gtgtatgaca tagcagaact ttaaaagtgc
     tcatcattgg aaaacgttct tcggggcgaa aactctcaag gatcttaccg ctgttgagat
     ccagttcgat gtaacccact cgtgcaccca actgatcttc agcatctttt actttcacca
     gcgtttctgg gtgagcaaaa acaggaaggc aaaatgccgc aaaaaaggga ataagggcga
     cacggaaatg ttgaatactc atactcttcc tttttcaata ttattgaagc atttatcagg
     gttattgtct catgagcgga tacatatttg aatgtattta gaaaaataaa caaatagggg
     ttccgcgcac atttccccga aaagtgccac ctgtatgcgg tgtgaaatac cgcacagatg
     cgtaaggaga aaataccgca tcaggcgaaa ttgtaaacgt taatattttg ttaaaattcg
     cgttaaatat ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     atggcccact acgtgaacca tcacccaaat caagtttttt gcggtcgagg tgccgtaaag
     ctctaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     cgtccattcg ccattcaggc tgcgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     gctattacgc cagctggcga aagggggatg tgctgcaagg cgattaagtt gggtaacgcc
     agggttttcc cagtcacgac gttgtaaaac gacggccagt gaattgtaat acgactcact
     ata
//