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ID   PGEX1LAMT  preliminary; circular DNA; SYN; 4947 BP.
XX
AC   IG1070; M21676; M97937;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGEX-1lambdaT - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pGEX-1lambdaT)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (p4.5)(pS)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-1 4941bp
FT                   2. lambda
FT                   -> pGEX-1lambdaT 4947bp"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-EcoRI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum
FT                   glutathione S-transferase (GST)"
XX
SQ   Sequence 4947 BP; 1223 A; 1193 C; 1283 G; 1248 T; 0 other;
     acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg gttccgcgtg gatccccgga attcatcgtg actgactgac
     gatctgcctc gcgcgtttcg gtgatgacgg tgaaaacctc tgacacatgc agctcccgga
     gacggtcaca gcttgtctgt aagcggatgc cgggagcaga caagcccgtc agggcgcgtc
     agcgggtgtt ggcgggtgtc ggggcgcagc catgacccag tcacgtagcg atagcggagt
     gtataattct tgaagacgaa agggcctcgt gatacgccta tttttatagg ttaatgtcat
     gataataatg gtttcttaga cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc
     tatttgttta tttttctaaa tacattcaaa tatgtatccg ctcatgagac aataaccctg
     ataaatgctt caataatatt gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc
     ccttattccc ttttttgcgg cattttgcct tcctgttttt gctcacccag aaacgctggt
     gaaagtaaaa gatgctgaag atcagttggg tgcacgagtg ggttacatcg aactggatct
     caacagcggt aagatccttg agagttttcg ccccgaagaa cgttttccaa tgatgagcac
     ttttaaagtt ctgctatgtg gcgcggtatt atcccgtgtt gacgccgggc aagagcaact
     cggtcgccgc atacactatt ctcagaatga cttggttgag tactcaccag tcacagaaaa
     gcatcttacg gatggcatga cagtaagaga attatgcagt gctgccataa ccatgagtga
     taacactgcg gccaacttac ttctgacaac gatcggagga ccgaaggagc taaccgcttt
     tttgcacaac atgggggatc atgtaactcg ccttgatcgt tgggaaccgg agctgaatga
     agccatacca aacgacgagc gtgacaccac gatgcctgca gcaatggcaa caacgttgcg
     caaactatta actggcgaac tacttactct agcttcccgg caacaattaa tagactggat
     ggaggcggat aaagttgcag gaccacttct gcgctcggcc cttccggctg gctggtttat
     tgctgataaa tctggagccg gtgagcgtgg gtctcgcggt atcattgcag cactggggcc
     agatggtaag ccctcccgta tcgtagttat ctacacgacg gggagtcagg caactatgga
     tgaacgaaat agacagatcg ctgagatagg tgcctcactg attaagcatt ggtaactgtc
     agaccaagtt tactcatata tactttagat tgatttaaaa cttcattttt aatttaaaag
     gatctaggtg aagatccttt ttgataatct catgaccaaa atcccttaac gtgagttttc
     gttccactga gcgtcagacc ccgtagaaaa gatcaaagga tcttcttgag atcctttttt
     tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt
     gccggatcaa gagctaccaa ctctttttcc gaaggtaact ggcttcagca gagcgcagat
     accaaatact gtccttctag tgtagccgta gttaggccac cacttcaaga actctgtagc
     accgcctaca tacctcgctc tgctaatcct gttaccagtg gctgctgcca gtggcgataa
     gtcgtgtctt accgggttgg actcaagacg atagttaccg gataaggcgc agcggtcggg
     ctgaacgggg ggttcgtgca cacagcccag cttggagcga acgacctaca ccgaactgag
     atacctacag cgtgagctat gagaaagcgc cacgcttccc gaagggagaa aggcggacag
     gtatccggta agcggcaggg tcggaacagg agagcgcacg agggagcttc cagggggaaa
     cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt
     gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg
     gttcctggcc ttttgctggc cttttgctca catgttcttt cctgcgttat cccctgattc
     tgtggataac cgtattaccg cctttgagtg agctgatacc gctcgccgca gccgaacgac
     cgagcgcagc gagtcagtga gcgaggaagc ggaagagcgc ctgatgcggt attttctcct
     tacgcatctg tgcggtattt cacaccgcat aaattccgac accatcgaat ggtgcaaaac
     ctttcgcggt atggcatgat agcgcccgga agagagtcaa ttcagggtgg tgaatgtgaa
     accagtaacg ttatacgatg tcgcagagta tgccggtgtc tcttatcaga ccgtttcccg
     cgtggtgaac caggccagcc acgtttctgc gaaaacgcgg gaaaaagtgg aagcggcgat
     ggcggagctg aattacattc ccaaccgcgt ggcacaacaa ctggcgggca aacagtcgtt
     gctgattggc gttgccacct ccagtctggc cctgcacgcg ccgtcgcaaa ttgtcgcggc
     gattaaatct cgcgccgatc aactgggtgc cagcgtggtg gtgtcgatgg tagaacgaag
     cggcgtcgaa gcctgtaaag cggcggtgca caatcttctc gcgcaacgcg tcagtgggct
     gatcattaac tatccgctgg atgaccagga tgccattgct gtggaagctg cctgcactaa
     tgttccggcg ttatttcttg atgtctctga ccagacaccc atcaacagta ttattttctc
     ccatgaagac ggtacgcgac tgggcgtgga gcatctggtc gcattgggtc accagcaaat
     cgcgctgtta gcgggcccat taagttctgt ctcggcgcgt ctgcgtctgg ctggctggca
     taaatatctc actcgcaatc aaattcagcc gatagcggaa cgggaaggcg actggagtgc
     catgtccggt tttcaacaaa ccatgcaaat gctgaatgag ggcatcgttc ccactgcgat
     gctggttgcc aacgatcaga tggcgctggg cgcaatgcgc gccattaccg agtccgggct
     gcgcgttggt gcggatatct cggtagtggg atacgacgat accgaagaca gctcatgtta
     tatcccgccg ttaaccacca tcaaacagga ttttcgcctg ctggggcaaa ccagcgtgga
     ccgcttgctg caactctctc agggccaggc ggtgaagggc aatcagctgt tgcccgtctc
     actggtgaaa agaaaaacca ccctggcgcc caatacgcaa accgcctctc cccgcgcgtt
     ggccgattca ttaatgcagc tggcacgaca ggtttcccga ctggaaagcg ggcagtgagc
     gcaacgcaat taatgtgagt tagctcactc attaggcacc ccaggcttta cactttatgc
     ttccggctcg tatgttgtgt ggaattgtga gcggataaca atttcacaca ggaaacagct
     atgaccatga ttacggattc actggccgtc gttttacaac gtcgtgactg ggaaaaccct
     ggcgttaccc aacttaatcg ccttgcagca catccccctt tcgccagctg gcgtaatagc
     gaagaggccc gcaccgatcg cccttcccaa cagttgcgca gcctgaatgg cgaatggcgc
     tttgcctggt ttccggcacc agaagcggtg ccggaaagct ggctggagtg cgatcttcct
     gaggccgata ctgtcgtcgt cccctcaaac tggcagatgc acggttacga tgcgcccatc
     tacaccaacg taacctatcc cattacggtc aatccgccgt ttgttcccac ggagaatccg
     acgggttgtt actcgctcac atttaatgtt gatgaaagct ggctacagga aggccagacg
     cgaattattt ttgatggcgt tggaatt
//