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ID PGEX2T preliminary; circular DNA; SYN; 4948 BP.
XX
AC A01438; A01578; IG1071; M21676; M97937;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pGEX-2T - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from pSj1 & pIC19H
RC pSj10 from plasmid & ptac or ptac12deltaEco
RC p4.5 from pSj5 & pMC9
RC pSj10Bam7Stop7 from pSj10
RC pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC pGEX-3X from pGEX-1 & oligo, factor Xa site
RA Smith D.B., Johnson K.S.;
RT "Single-step purification of polypeptides expressed in Escherichia
RT coli as fusions with glutathione S-transferase";
RL Gene 67:31-40(1988).
XX
CC thrombin or factor Xa protease sites to cleave protein from fusion.
CC pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC NM (pGEX-2T)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)(ATCC)
CC HO (E.coli)
CC CP ()
CC FN (expression)(cloning directional)
CC SE (affinity)(color yellow)
CC PA (p4.5)(pS)(pGEX-1)
CC BR (pGEX-3X)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEX-1 BamHI 4941bp 915..915
FT 2. oligo BamHI-BamHI 16bp ggatctggttccgcgt,
FT \ thrombin site
FT -> pGEX-2T 4948bp"
FT misc_binding 0..0
FT /note="SIT unique BspMI"
FT promoter 0..0
FT /note="PRO E. coli tac (trp and lac)"
FT CDS 0..0
FT /note="GEN Schistosoma japonicum
FT glutathione S-transferase (GST)"
FT misc_binding 0..0
FT /note="SIT unique BalI"
FT misc_binding 0..0
FT /note="MCS unique BamHI-SmaI-EcoRI"
FT misc_feature 0..0
FT /note="stop codons"
FT misc_binding 0..0
FT /note="SIT unique Tth1111"
FT misc_binding 0..0
FT /note="SIT unique AatII"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT unique PstI"
FT misc_binding 0..0
FT /note="SIT unique AlwNI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="REP E. coli lacI repressor gene"
FT misc_binding 0..0
FT /note="SIT unique MluI"
FT misc_binding 0..0
FT /note="SIT unique BstEII"
FT misc_binding 0..0
FT /note="SIT unique ApaI"
FT misc_binding 0..0
FT /note="SIT unique BssHII"
FT misc_binding 0..0
FT /note="SIT unique EcoRV"
FT misc_binding 0..0
FT /note="SIT unique NarI"
XX
SQ Sequence 4948 BP; 1223 A; 1193 C; 1284 G; 1248 T; 0 other;
acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
atcctccaaa atcggatctg gttccgcgtg gatccccggg aattcatcgt gactgactga
cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
ttacgcatct gtgcggtatt tcacaccgca taaattccga caccatcgaa tggtgcaaaa
cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
gcgaattatt tttgatggcg ttggaatt
//