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ID   PGEX2T     preliminary; circular DNA; SYN; 4948 BP.
XX
AC   A01438; A01578; IG1071; M21676; M97937;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGEX-2T - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pGEX-2T)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)(ATCC)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (p4.5)(pS)(pGEX-1)
CC   BR (pGEX-3X)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-1 BamHI 4941bp 915..915
FT                   2. oligo BamHI-BamHI 16bp ggatctggttccgcgt,
FT                   \ thrombin site
FT                   -> pGEX-2T 4948bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BspMI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum
FT                   glutathione S-transferase (GST)"
FT   misc_binding    0..0
FT                   /note="SIT unique BalI"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-SmaI-EcoRI"
FT   misc_feature    0..0
FT                   /note="stop codons"
FT   misc_binding    0..0
FT                   /note="SIT unique Tth1111"
FT   misc_binding    0..0
FT                   /note="SIT unique AatII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique AlwNI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
XX
SQ   Sequence 4948 BP; 1223 A; 1193 C; 1284 G; 1248 T; 0 other;
     acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg gttccgcgtg gatccccggg aattcatcgt gactgactga
     cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct ctgacacatg cagctcccgg
     agacggtcac agcttgtctg taagcggatg ccgggagcag acaagcccgt cagggcgcgt
     cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca gtcacgtagc gatagcggag
     tgtataattc ttgaagacga aagggcctcg tgatacgcct atttttatag gttaatgtca
     tgataataat ggtttcttag acgtcaggtg gcacttttcg gggaaatgtg cgcggaaccc
     ctatttgttt atttttctaa atacattcaa atatgtatcc gctcatgaga caataaccct
     gataaatgct tcaataatat tgaaaaagga agagtatgag tattcaacat ttccgtgtcg
     cccttattcc cttttttgcg gcattttgcc ttcctgtttt tgctcaccca gaaacgctgg
     tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt gggttacatc gaactggatc
     tcaacagcgg taagatcctt gagagttttc gccccgaaga acgttttcca atgatgagca
     cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt tgacgccggg caagagcaac
     tcggtcgccg catacactat tctcagaatg acttggttga gtactcacca gtcacagaaa
     agcatcttac ggatggcatg acagtaagag aattatgcag tgctgccata accatgagtg
     ataacactgc ggccaactta cttctgacaa cgatcggagg accgaaggag ctaaccgctt
     ttttgcacaa catgggggat catgtaactc gccttgatcg ttgggaaccg gagctgaatg
     aagccatacc aaacgacgag cgtgacacca cgatgcctgc agcaatggca acaacgttgc
     gcaaactatt aactggcgaa ctacttactc tagcttcccg gcaacaatta atagactgga
     tggaggcgga taaagttgca ggaccacttc tgcgctcggc ccttccggct ggctggttta
     ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg tatcattgca gcactggggc
     cagatggtaa gccctcccgt atcgtagtta tctacacgac ggggagtcag gcaactatgg
     atgaacgaaa tagacagatc gctgagatag gtgcctcact gattaagcat tggtaactgt
     cagaccaagt ttactcatat atactttaga ttgatttaaa acttcatttt taatttaaaa
     ggatctaggt gaagatcctt tttgataatc tcatgaccaa aatcccttaa cgtgagtttt
     cgttccactg agcgtcagac cccgtagaaa agatcaaagg atcttcttga gatccttttt
     ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc gctaccagcg gtggtttgtt
     tgccggatca agagctacca actctttttc cgaaggtaac tggcttcagc agagcgcaga
     taccaaatac tgtccttcta gtgtagccgt agttaggcca ccacttcaag aactctgtag
     caccgcctac atacctcgct ctgctaatcc tgttaccagt ggctgctgcc agtggcgata
     agtcgtgtct taccgggttg gactcaagac gatagttacc ggataaggcg cagcggtcgg
     gctgaacggg gggttcgtgc acacagccca gcttggagcg aacgacctac accgaactga
     gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc cgaagggaga aaggcggaca
     ggtatccggt aagcggcagg gtcggaacag gagagcgcac gagggagctt ccagggggaa
     acgcctggta tctttatagt cctgtcgggt ttcgccacct ctgacttgag cgtcgatttt
     tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc cagcaacgcg gcctttttac
     ggttcctggc cttttgctgg ccttttgctc acatgttctt tcctgcgtta tcccctgatt
     ctgtggataa ccgtattacc gcctttgagt gagctgatac cgctcgccgc agccgaacga
     ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg cctgatgcgg tattttctcc
     ttacgcatct gtgcggtatt tcacaccgca taaattccga caccatcgaa tggtgcaaaa
     cctttcgcgg tatggcatga tagcgcccgg aagagagtca attcagggtg gtgaatgtga
     aaccagtaac gttatacgat gtcgcagagt atgccggtgt ctcttatcag accgtttccc
     gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg ggaaaaagtg gaagcggcga
     tggcggagct gaattacatt cccaaccgcg tggcacaaca actggcgggc aaacagtcgt
     tgctgattgg cgttgccacc tccagtctgg ccctgcacgc gccgtcgcaa attgtcgcgg
     cgattaaatc tcgcgccgat caactgggtg ccagcgtggt ggtgtcgatg gtagaacgaa
     gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct cgcgcaacgc gtcagtgggc
     tgatcattaa ctatccgctg gatgaccagg atgccattgc tgtggaagct gcctgcacta
     atgttccggc gttatttctt gatgtctctg accagacacc catcaacagt attattttct
     cccatgaaga cggtacgcga ctgggcgtgg agcatctggt cgcattgggt caccagcaaa
     tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg tctgcgtctg gctggctggc
     ataaatatct cactcgcaat caaattcagc cgatagcgga acgggaaggc gactggagtg
     ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga gggcatcgtt cccactgcga
     tgctggttgc caacgatcag atggcgctgg gcgcaatgcg cgccattacc gagtccgggc
     tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga taccgaagac agctcatgtt
     atatcccgcc gttaaccacc atcaaacagg attttcgcct gctggggcaa accagcgtgg
     accgcttgct gcaactctct cagggccagg cggtgaaggg caatcagctg ttgcccgtct
     cactggtgaa aagaaaaacc accctggcgc ccaatacgca aaccgcctct ccccgcgcgt
     tggccgattc attaatgcag ctggcacgac aggtttcccg actggaaagc gggcagtgag
     cgcaacgcaa ttaatgtgag ttagctcact cattaggcac cccaggcttt acactttatg
     cttccggctc gtatgttgtg tggaattgtg agcggataac aatttcacac aggaaacagc
     tatgaccatg attacggatt cactggccgt cgttttacaa cgtcgtgact gggaaaaccc
     tggcgttacc caacttaatc gccttgcagc acatccccct ttcgccagct ggcgtaatag
     cgaagaggcc cgcaccgatc gcccttccca acagttgcgc agcctgaatg gcgaatggcg
     ctttgcctgg tttccggcac cagaagcggt gccggaaagc tggctggagt gcgatcttcc
     tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg cacggttacg atgcgcccat
     ctacaccaac gtaacctatc ccattacggt caatccgccg tttgttccca cggagaatcc
     gacgggttgt tactcgctca catttaatgt tgatgaaagc tggctacagg aaggccagac
     gcgaattatt tttgatggcg ttggaatt
//