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ID   PGEX3      preliminary; circular DNA; SYN; 4932 BP.
XX
AC   M21676;
XX
DT   02-MAY-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGEX-3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4932
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
RN   [2]
RP   1-4932
RC   pGEX-3 from pGEX-1
RA   Davern K.M.;
RT   ;
RL   Unpublished (1991).
XX
CC   NCBI gi: 208304
CC   NM (pGEX-3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)(expression)
CC   SE ()
CC   PA (pGEX-1)(E.coli)(S.japonicum)
CC   BR ()
CC   OF ()
CC   OR (29 bp upstream of BamHI site)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-1 4941bp
FT                   -> pGEX-3 4932bp"
FT   misc_feature    914..919
FT                   /note="SIT BamHI [2]"
FT   misc_feature    919..924
FT                   /note="SIT SmaI [2]"
FT   misc_feature    924..929
FT                   /note="SIT EcoRI [2]"
FT   CDS             258..944
FT                   /note="GEN Schistosoma japonicum
FT                   glutathione-S-transferase gene (Sj26), 5' end,
FT                   transformed [2]"
FT   misc_binding    0..0
FT                   /note="SIT factor X protease cleavage site"
FT   misc_feature    0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 4932 BP; 1222 A; 1190 C; 1278 G; 1242 T; 0 other;
     agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatccc cgggaattca tcgtgactga ctgacgatct gcctcgcgcg
     tttcggtgat gacggtgaaa acctctgaca catgcagctc ccggagacgg tcacagcttg
     tctgtaagcg gatgccggga gcagacaagc ccgtcagggc gcgtcagcgg gtgttggcgg
     gtgtcggggc gcagccatga cccagtcacg tagcgatagc ggagtgtata attcttgaag
     acgaaagggc ctcgtgatac gcctattttt ataggttaat gtcatgataa taatggtttc
     ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga acccctattt gtttattttt
     ctaaatacat tcaaatatgt atccgctcat gagacaataa ccctgataaa tgcttcaata
     atattgaaaa aggaagagta tgagtattca acatttccgt gtcgccctta ttcccttttt
     tgcggcattt tgccttcctg tttttgctca cccagaaacg ctggtgaaag taaaagatgc
     tgaagatcag ttgggtgcac gagtgggtta catcgaactg gatctcaaca gcggtaagat
     ccttgagagt tttcgccccg aagaacgttt tccaatgatg agcactttta aagttctgct
     atgtggcgcg gtattatccc gtgttgacgc cgggcaagag caactcggtc gccgcataca
     ctattctcag aatgacttgg ttgagtactc accagtcaca gaaaagcatc ttacggatgg
     catgacagta agagaattat gcagtgctgc cataaccatg agtgataaca ctgcggccaa
     cttacttctg acaacgatcg gaggaccgaa ggagctaacc gcttttttgc acaacatggg
     ggatcatgta actcgccttg atcgttggga accggagctg aatgaagcca taccaaacga
     cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg ttgcgcaaac tattaactgg
     cgaactactt actctagctt cccggcaaca attaatagac tggatggagg cggataaagt
     tgcaggacca cttctgcgct cggcccttcc ggctggctgg tttattgctg ataaatctgg
     agccggtgag cgtgggtctc gcggtatcat tgcagcactg gggccagatg gtaagccctc
     ccgtatcgta gttatctaca cgacggggag tcaggcaact atggatgaac gaaatagaca
     gatcgctgag ataggtgcct cactgattaa gcattggtaa ctgtcagacc aagtttactc
     atatatactt tagattgatt taaaacttca tttttaattt aaaaggatct aggtgaagat
     cctttttgat aatctcatga ccaaaatccc ttaacgtgag ttttcgttcc actgagcgtc
     agaccccgta gaaaagatca aaggatcttc ttgagatcct ttttttctgc gcgtaatctg
     ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt tgtttgccgg atcaagagct
     accaactctt tttccgaagg taactggctt cagcagagcg cagataccaa atactgtcct
     tctagtgtag ccgtagttag gccaccactt caagaactct gtagcaccgc ctacatacct
     cgctctgcta atcctgttac cagtggctgc tgccagtggc gataagtcgt gtcttaccgg
     gttggactca agacgatagt taccggataa ggcgcagcgg tcgggctgaa cggggggttc
     gtgcacacag cccagcttgg agcgaacgac ctacaccgaa ctgagatacc tacagcgtga
     gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg gacaggtatc cggtaagcgg
     cagggtcgga acaggagagc gcacgaggga gcttccaggg ggaaacgcct ggtatcttta
     tagtcctgtc gggtttcgcc acctctgact tgagcgtcga tttttgtgat gctcgtcagg
     ggggcggagc ctatggaaaa acgccagcaa cgcggccttt ttacggttcc tggccttttg
     ctggcctttt gctcacatgt tctttcctgc gttatcccct gattctgtgg ataaccgtat
     taccgccttt gagtgagctg ataccgctcg ccgcagccga acgaccgagc gcagcgagtc
     agtgagcgag gaagcggaag agcgcctgat gcggtatttt ctccttacgc atctgtgcgg
     tatttcacac cgcataaatt ccgacaccat cgaatggtgc aaaacctttc gcggtatggc
     atgatagcgc ccggaagaga gtcaattcag ggtggtgaat gtgaaaccag taacgttata
     cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt tcccgcgtgg tgaaccaggc
     cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg gcgatggcgg agctgaatta
     cattcccaac cgcgtggcac aacaactggc gggcaaacag tcgttgctga ttggcgttgc
     cacctccagt ctggccctgc acgcgccgtc gcaaattgtc gcggcgatta aatctcgcgc
     cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa cgaagcggcg tcgaagcctg
     taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt gggctgatca ttaactatcc
     gctggatgac caggatgcca ttgctgtgga agctgcctgc actaatgttc cggcgttatt
     tcttgatgtc tctgaccaga cacccatcaa cagtattatt ttctcccatg aagacggtac
     gcgactgggc gtggagcatc tggtcgcatt gggtcaccag caaatcgcgc tgttagcggg
     cccattaagt tctgtctcgg cgcgtctgcg tctggctggc tggcataaat atctcactcg
     caatcaaatt cagccgatag cggaacggga aggcgactgg agtgccatgt ccggttttca
     acaaaccatg caaatgctga atgagggcat cgttcccact gcgatgctgg ttgccaacga
     tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc gggctgcgcg ttggtgcgga
     tatctcggta gtgggatacg acgataccga agacagctca tgttatatcc cgccgtcaac
     caccatcaaa caggattttc gcctgctggg gcaaaccagc gtggaccgct tgctgcaact
     ctctcagggc caggcggtga agggcaatca gctgttgccc gtctcactgg tgaaaagaaa
     aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa accctggcgt tacccaactt
     aatcgccttg cagcacatcc ccctttcgcc agctggcgta atagcgaaga ggcccgcacc
     gatcgccctt cccaacagtt gcgcagcctg aatggcgaat ggcgctttgc ctggtttccg
     gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
     tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     ggcgttggaa tt
//