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ID PGEX3X preliminary; circular DNA; SYN; 4952 BP.
XX
AC A01439; A01580; IG1073; M21676; M97937;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pGEX-3X - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from pSj1 & pIC19H
RC pSj10 from plasmid & ptac or ptac12deltaEco
RC p4.5 from pSj5 & pMC9
RC pSj10Bam7Stop7 from pSj10
RC pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC pGEX-3X from pGEX-1 & oligo, factor Xa site
RA Smith D.B., Johnson K.S.;
RT "Single-step purification of polypeptides expressed in Escherichia
RT coli as fusions with glutathione S-transferase";
RL Gene 67:31-40(1988).
XX
CC thrombin or factor Xa protease sites to cleave protein from fusion.
CC pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC NM (pGEX-3X)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)(ATCC)
CC HO (E.coli)
CC CP ()
CC FN (expression)(cloning directional)
CC SE (affinity)(color yellow)
CC PA (p4.5)(pS)(pGEX-1)
CC BR (pGEX-2T)
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEX-1 BamHI 4941bp
FT 2. oligo BamHI-BamHI 20bp ggatctgatcgaaggtcgtg,
FT \ factor Xa
FT -> pGEX-3X 4952bp"
FT misc_binding 0..0
FT /note="MCS unique BamHI-SmaI-EcoRI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT unique PstI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="REP E. coli lacI repressor gene"
FT misc_binding 0..0
FT /note="SIT unique MluI"
FT misc_binding 0..0
FT /note="SIT unique BstEII"
FT misc_binding 0..0
FT /note="SIT unique ApaI"
FT misc_binding 0..0
FT /note="SIT unique BssHII"
FT misc_binding 0..0
FT /note="SIT unique EcoRV"
FT misc_binding 0..0
FT /note="SIT unique NarI"
FT promoter 0..0
FT /note="PRO E. coli tac (trp and lac)"
FT CDS 0..0
FT /note="GEN Schistosoma japonicum
FT glutathione S-transferase (GST)"
XX
SQ Sequence 4952 BP; 1226 A; 1192 C; 1286 G; 1248 T; 0 other;
agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
atcctccaaa atcggatctg atcgaaggtc gtgggatccc cgggaattca tcgtgactga
ctgacgatct gcctcgcgcg tttcggtgat gacggtgaaa acctctgaca catgcagctc
ccggagacgg tcacagcttg tctgtaagcg gatgccggga gcagacaagc ccgtcagggc
gcgtcagcgg gtgttggcgg gtgtcggggc gcagccatga cccagtcacg tagcgatagc
ggagtgtata attcttgaag acgaaagggc ctcgtgatac gcctattttt ataggttaat
gtcatgataa taatggtttc ttagacgtca ggtggcactt ttcggggaaa tgtgcgcgga
acccctattt gtttattttt ctaaatacat tcaaatatgt atccgctcat gagacaataa
ccctgataaa tgcttcaata atattgaaaa aggaagagta tgagtattca acatttccgt
gtcgccctta ttcccttttt tgcggcattt tgccttcctg tttttgctca cccagaaacg
ctggtgaaag taaaagatgc tgaagatcag ttgggtgcac gagtgggtta catcgaactg
gatctcaaca gcggtaagat ccttgagagt tttcgccccg aagaacgttt tccaatgatg
agcactttta aagttctgct atgtggcgcg gtattatccc gtgttgacgc cgggcaagag
caactcggtc gccgcataca ctattctcag aatgacttgg ttgagtactc accagtcaca
gaaaagcatc ttacggatgg catgacagta agagaattat gcagtgctgc cataaccatg
agtgataaca ctgcggccaa cttacttctg acaacgatcg gaggaccgaa ggagctaacc
gcttttttgc acaacatggg ggatcatgta actcgccttg atcgttggga accggagctg
aatgaagcca taccaaacga cgagcgtgac accacgatgc ctgcagcaat ggcaacaacg
ttgcgcaaac tattaactgg cgaactactt actctagctt cccggcaaca attaatagac
tggatggagg cggataaagt tgcaggacca cttctgcgct cggcccttcc ggctggctgg
tttattgctg ataaatctgg agccggtgag cgtgggtctc gcggtatcat tgcagcactg
gggccagatg gtaagccctc ccgtatcgta gttatctaca cgacggggag tcaggcaact
atggatgaac gaaatagaca gatcgctgag ataggtgcct cactgattaa gcattggtaa
ctgtcagacc aagtttactc atatatactt tagattgatt taaaacttca tttttaattt
aaaaggatct aggtgaagat cctttttgat aatctcatga ccaaaatccc ttaacgtgag
ttttcgttcc actgagcgtc agaccccgta gaaaagatca aaggatcttc ttgagatcct
ttttttctgc gcgtaatctg ctgcttgcaa acaaaaaaac caccgctacc agcggtggtt
tgtttgccgg atcaagagct accaactctt tttccgaagg taactggctt cagcagagcg
cagataccaa atactgtcct tctagtgtag ccgtagttag gccaccactt caagaactct
gtagcaccgc ctacatacct cgctctgcta atcctgttac cagtggctgc tgccagtggc
gataagtcgt gtcttaccgg gttggactca agacgatagt taccggataa ggcgcagcgg
tcgggctgaa cggggggttc gtgcacacag cccagcttgg agcgaacgac ctacaccgaa
ctgagatacc tacagcgtga gctatgagaa agcgccacgc ttcccgaagg gagaaaggcg
gacaggtatc cggtaagcgg cagggtcgga acaggagagc gcacgaggga gcttccaggg
ggaaacgcct ggtatcttta tagtcctgtc gggtttcgcc acctctgact tgagcgtcga
tttttgtgat gctcgtcagg ggggcggagc ctatggaaaa acgccagcaa cgcggccttt
ttacggttcc tggccttttg ctggcctttt gctcacatgt tctttcctgc gttatcccct
gattctgtgg ataaccgtat taccgccttt gagtgagctg ataccgctcg ccgcagccga
acgaccgagc gcagcgagtc agtgagcgag gaagcggaag agcgcctgat gcggtatttt
ctccttacgc atctgtgcgg tatttcacac cgcataaatt ccgacaccat cgaatggtgc
aaaacctttc gcggtatggc atgatagcgc ccggaagaga gtcaattcag ggtggtgaat
gtgaaaccag taacgttata cgatgtcgca gagtatgccg gtgtctctta tcagaccgtt
tcccgcgtgg tgaaccaggc cagccacgtt tctgcgaaaa cgcgggaaaa agtggaagcg
gcgatggcgg agctgaatta cattcccaac cgcgtggcac aacaactggc gggcaaacag
tcgttgctga ttggcgttgc cacctccagt ctggccctgc acgcgccgtc gcaaattgtc
gcggcgatta aatctcgcgc cgatcaactg ggtgccagcg tggtggtgtc gatggtagaa
cgaagcggcg tcgaagcctg taaagcggcg gtgcacaatc ttctcgcgca acgcgtcagt
gggctgatca ttaactatcc gctggatgac caggatgcca ttgctgtgga agctgcctgc
actaatgttc cggcgttatt tcttgatgtc tctgaccaga cacccatcaa cagtattatt
ttctcccatg aagacggtac gcgactgggc gtggagcatc tggtcgcatt gggtcaccag
caaatcgcgc tgttagcggg cccattaagt tctgtctcgg cgcgtctgcg tctggctggc
tggcataaat atctcactcg caatcaaatt cagccgatag cggaacggga aggcgactgg
agtgccatgt ccggttttca acaaaccatg caaatgctga atgagggcat cgttcccact
gcgatgctgg ttgccaacga tcagatggcg ctgggcgcaa tgcgcgccat taccgagtcc
gggctgcgcg ttggtgcgga tatctcggta gtgggatacg acgataccga agacagctca
tgttatatcc cgccgttaac caccatcaaa caggattttc gcctgctggg gcaaaccagc
gtggaccgct tgctgcaact ctctcagggc caggcggtga agggcaatca gctgttgccc
gtctcactgg tgaaaagaaa aaccaccctg gcgcccaata cgcaaaccgc ctctccccgc
gcgttggccg attcattaat gcagctggca cgacaggttt cccgactgga aagcgggcag
tgagcgcaac gcaattaatg tgagttagct cactcattag gcaccccagg ctttacactt
tatgcttccg gctcgtatgt tgtgtggaat tgtgagcgga taacaatttc acacaggaaa
cagctatgac catgattacg gattcactgg ccgtcgtttt acaacgtcgt gactgggaaa
accctggcgt tacccaactt aatcgccttg cagcacatcc ccctttcgcc agctggcgta
atagcgaaga ggcccgcacc gatcgccctt cccaacagtt gcgcagcctg aatggcgaat
ggcgctttgc ctggtttccg gcaccagaag cggtgccgga aagctggctg gagtgcgatc
ttcctgaggc cgatactgtc gtcgtcccct caaactggca gatgcacggt tacgatgcgc
ccatctacac caacgtaacc tatcccatta cggtcaatcc gccgtttgtt cccacggaga
atccgacggg ttgttactcg ctcacattta atgttgatga aagctggcta caggaaggcc
agacgcgaat tatttttgat ggcgttggaa tt
//