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ID   PGEX4T1    preliminary; circular DNA; SYN; 4969 BP.
XX
AC   IG1074; M21676; M97937;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGEX-4T1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pGEX-4T1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (pGEX-2T)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-4
FT                   2. oligo, thrombin site
FT                   -> pGEX-4T-1 4969bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-SalI-XhoI-NotI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum
FT                   glutathione S-transferase (GST)"
FT   misc_binding    0..0
FT                   /note="SIT thrombin"
XX
SQ   Sequence 4969 BP; 1225 A; 1202 C; 1292 G; 1250 T; 0 other;
     acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg gttccgcgtg gatccccgga attcccgggt cgactcgagc
     ggccgcatcg tgactgactg acgatctgcc tcgcgcgttt cggtgatgac ggtgaaaacc
     tctgacacat gcagctcccg gagacggtca cagcttgtct gtaagcggat gccgggagca
     gacaagcccg tcagggcgcg tcagcgggtg ttggcgggtg tcggggcgca gccatgaccc
     agtcacgtag cgatagcgga gtgtataatt cttgaagacg aaagggcctc gtgatacgcc
     tatttttata ggttaatgtc atgataataa tggtttctta gacgtcaggt ggcacttttc
     ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca aatatgtatc
     cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg aagagtatga
     gtattcaaca tttccgtgtc gcccttattc ccttttttgc ggcattttgc cttcctgttt
     ttgctcaccc agaaacgctg gtgaaagtaa aagatgctga agatcagttg ggtgcacgag
     tgggttacat cgaactggat ctcaacagcg gtaagatcct tgagagtttt cgccccgaag
     aacgttttcc aatgatgagc acttttaaag ttctgctatg tggcgcggta ttatcccgtg
     ttgacgccgg gcaagagcaa ctcggtcgcc gcatacacta ttctcagaat gacttggttg
     agtactcacc agtcacagaa aagcatctta cggatggcat gacagtaaga gaattatgca
     gtgctgccat aaccatgagt gataacactg cggccaactt acttctgaca acgatcggag
     gaccgaagga gctaaccgct tttttgcaca acatggggga tcatgtaact cgccttgatc
     gttgggaacc ggagctgaat gaagccatac caaacgacga gcgtgacacc acgatgcctg
     cagcaatggc aacaacgttg cgcaaactat taactggcga actacttact ctagcttccc
     ggcaacaatt aatagactgg atggaggcgg ataaagttgc aggaccactt ctgcgctcgg
     cccttccggc tggctggttt attgctgata aatctggagc cggtgagcgt gggtctcgcg
     gtatcattgc agcactgggg ccagatggta agccctcccg tatcgtagtt atctacacga
     cggggagtca ggcaactatg gatgaacgaa atagacagat cgctgagata ggtgcctcac
     tgattaagca ttggtaactg tcagaccaag tttactcata tatactttag attgatttaa
     aacttcattt ttaatttaaa aggatctagg tgaagatcct ttttgataat ctcatgacca
     aaatccctta acgtgagttt tcgttccact gagcgtcaga ccccgtagaa aagatcaaag
     gatcttcttg agatcctttt tttctgcgcg taatctgctg cttgcaaaca aaaaaaccac
     cgctaccagc ggtggtttgt ttgccggatc aagagctacc aactcttttt ccgaaggtaa
     ctggcttcag cagagcgcag ataccaaata ctgtccttct agtgtagccg tagttaggcc
     accacttcaa gaactctgta gcaccgccta catacctcgc tctgctaatc ctgttaccag
     tggctgctgc cagtggcgat aagtcgtgtc ttaccgggtt ggactcaaga cgatagttac
     cggataaggc gcagcggtcg ggctgaacgg ggggttcgtg cacacagccc agcttggagc
     gaacgaccta caccgaactg agatacctac agcgtgagct atgagaaagc gccacgcttc
     ccgaagggag aaaggcggac aggtatccgg taagcggcag ggtcggaaca ggagagcgca
     cgagggagct tccaggggga aacgcctggt atctttatag tcctgtcggg tttcgccacc
     tctgacttga gcgtcgattt ttgtgatgct cgtcaggggg gcggagccta tggaaaaacg
     ccagcaacgc ggccttttta cggttcctgg ccttttgctg gccttttgct cacatgttct
     ttcctgcgtt atcccctgat tctgtggata accgtattac cgcctttgag tgagctgata
     ccgctcgccg cagccgaacg accgagcgca gcgagtcagt gagcgaggaa gcggaagagc
     gcctgatgcg gtattttctc cttacgcatc tgtgcggtat ttcacaccgc ataaattccg
     acaccatcga atggtgcaaa acctttcgcg gtatggcatg atagcgcccg gaagagagtc
     aattcagggt ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag tatgccggtg
     tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct gcgaaaacgc
     gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc gtggcacaac
     aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg gccctgcacg
     cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt gccagcgtgg
     tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg cacaatcttc
     tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag gatgccattg
     ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct gaccagacac
     ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg gagcatctgg
     tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct gtctcggcgc
     gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag ccgatagcgg
     aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa atgctgaatg
     agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg ggcgcaatgc
     gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg ggatacgacg
     ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag gattttcgcc
     tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag gcggtgaagg
     gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg cccaatacgc
     aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga caggtttccc
     gactggaaag cgggcagtga gcgcaacgca attaatgtga gttagctcac tcattaggca
     ccccaggctt tacactttat gcttccggct cgtatgttgt gtggaattgt gagcggataa
     caatttcaca caggaaacag ctatgaccat gattacggat tcactggccg tcgttttaca
     acgtcgtgac tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc
     tttcgccagc tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg
     cagcctgaat ggcgaatggc gctttgcctg gtttccggca ccagaagcgg tgccggaaag
     ctggctggag tgcgatcttc ctgaggccga tactgtcgtc gtcccctcaa actggcagat
     gcacggttac gatgcgccca tctacaccaa cgtaacctat cccattacgg tcaatccgcc
     gtttgttccc acggagaatc cgacgggttg ttactcgctc acatttaatg ttgatgaaag
     ctggctacag gaaggccaga cgcgaattat ttttgatggc gttggaatt
//