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ID   PGEX4T2    preliminary; circular DNA; SYN; 4970 BP.
XX
AC   IG1075; M21676; M97937;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGEX-4T2 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pGEX-4T2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (pGEX-2T)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-4
FT                   2. oligo, thrombin site
FT                   -> pGEX-4T-2 4970bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-SalI-XhoI-NotI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum
FT                   glutathione S-transferase (GST)"
FT   misc_binding    0..0
FT                   /note="SIT thrombin"
XX
SQ   Sequence 4970 BP; 1226 A; 1202 C; 1292 G; 1250 T; 0 other;
     acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg gttccgcgtg gatccccagg aattcccggg tcgactcgag
     cggccgcatc gtgactgact gacgatctgc ctcgcgcgtt tcggtgatga cggtgaaaac
     ctctgacaca tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc
     agacaagccc gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc
     cagtcacgta gcgatagcgg agtgtataat tcttgaagac gaaagggcct cgtgatacgc
     ctatttttat aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt
     cggggaaatg tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat
     ccgctcatga gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg
     agtattcaac atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt
     tttgctcacc cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga
     gtgggttaca tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa
     gaacgttttc caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt
     gttgacgccg ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt
     gagtactcac cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc
     agtgctgcca taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga
     ggaccgaagg agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat
     cgttgggaac cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct
     gcagcaatgg caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc
     cggcaacaat taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg
     gcccttccgg ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc
     ggtatcattg cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg
     acggggagtc aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca
     ctgattaagc attggtaact gtcagaccaa gtttactcat atatacttta gattgattta
     aaacttcatt tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc
     aaaatccctt aacgtgagtt ttcgttccac tgagcgtcag accccgtaga aaagatcaaa
     ggatcttctt gagatccttt ttttctgcgc gtaatctgct gcttgcaaac aaaaaaacca
     ccgctaccag cggtggtttg tttgccggat caagagctac caactctttt tccgaaggta
     actggcttca gcagagcgca gataccaaat actgtccttc tagtgtagcc gtagttaggc
     caccacttca agaactctgt agcaccgcct acatacctcg ctctgctaat cctgttacca
     gtggctgctg ccagtggcga taagtcgtgt cttaccgggt tggactcaag acgatagtta
     ccggataagg cgcagcggtc gggctgaacg gggggttcgt gcacacagcc cagcttggag
     cgaacgacct acaccgaact gagataccta cagcgtgagc tatgagaaag cgccacgctt
     cccgaaggga gaaaggcgga caggtatccg gtaagcggca gggtcggaac aggagagcgc
     acgagggagc ttccaggggg aaacgcctgg tatctttata gtcctgtcgg gtttcgccac
     ctctgacttg agcgtcgatt tttgtgatgc tcgtcagggg ggcggagcct atggaaaaac
     gccagcaacg cggccttttt acggttcctg gccttttgct ggccttttgc tcacatgttc
     tttcctgcgt tatcccctga ttctgtggat aaccgtatta ccgcctttga gtgagctgat
     accgctcgcc gcagccgaac gaccgagcgc agcgagtcag tgagcgagga agcggaagag
     cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg cataaattcc
     gacaccatcg aatggtgcaa aacctttcgc ggtatggcat gatagcgccc ggaagagagt
     caattcaggg tggtgaatgt gaaaccagta acgttatacg atgtcgcaga gtatgccggt
     gtctcttatc agaccgtttc ccgcgtggtg aaccaggcca gccacgtttc tgcgaaaacg
     cgggaaaaag tggaagcggc gatggcggag ctgaattaca ttcccaaccg cgtggcacaa
     caactggcgg gcaaacagtc gttgctgatt ggcgttgcca cctccagtct ggccctgcac
     gcgccgtcgc aaattgtcgc ggcgattaaa tctcgcgccg atcaactggg tgccagcgtg
     gtggtgtcga tggtagaacg aagcggcgtc gaagcctgta aagcggcggt gcacaatctt
     ctcgcgcaac gcgtcagtgg gctgatcatt aactatccgc tggatgacca ggatgccatt
     gctgtggaag ctgcctgcac taatgttccg gcgttatttc ttgatgtctc tgaccagaca
     cccatcaaca gtattatttt ctcccatgaa gacggtacgc gactgggcgt ggagcatctg
     gtcgcattgg gtcaccagca aatcgcgctg ttagcgggcc cattaagttc tgtctcggcg
     cgtctgcgtc tggctggctg gcataaatat ctcactcgca atcaaattca gccgatagcg
     gaacgggaag gcgactggag tgccatgtcc ggttttcaac aaaccatgca aatgctgaat
     gagggcatcg ttcccactgc gatgctggtt gccaacgatc agatggcgct gggcgcaatg
     cgcgccatta ccgagtccgg gctgcgcgtt ggtgcggata tctcggtagt gggatacgac
     gataccgaag acagctcatg ttatatcccg ccgttaacca ccatcaaaca ggattttcgc
     ctgctggggc aaaccagcgt ggaccgcttg ctgcaactct ctcagggcca ggcggtgaag
     ggcaatcagc tgttgcccgt ctcactggtg aaaagaaaaa ccaccctggc gcccaatacg
     caaaccgcct ctccccgcgc gttggccgat tcattaatgc agctggcacg acaggtttcc
     cgactggaaa gcgggcagtg agcgcaacgc aattaatgtg agttagctca ctcattaggc
     accccaggct ttacacttta tgcttccggc tcgtatgttg tgtggaattg tgagcggata
     acaatttcac acaggaaaca gctatgacca tgattacgga ttcactggcc gtcgttttac
     aacgtcgtga ctgggaaaac cctggcgtta cccaacttaa tcgccttgca gcacatcccc
     ctttcgccag ctggcgtaat agcgaagagg cccgcaccga tcgcccttcc caacagttgc
     gcagcctgaa tggcgaatgg cgctttgcct ggtttccggc accagaagcg gtgccggaaa
     gctggctgga gtgcgatctt cctgaggccg atactgtcgt cgtcccctca aactggcaga
     tgcacggtta cgatgcgccc atctacacca acgtaaccta tcccattacg gtcaatccgc
     cgtttgttcc cacggagaat ccgacgggtt gttactcgct cacatttaat gttgatgaaa
     gctggctaca ggaaggccag acgcgaatta tttttgatgg cgttggaatt
//