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ID   PGEX4T3    preliminary; circular DNA; SYN; 4968 BP.
XX
AC   IG1076; M21676; M97937;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGEX-4T3 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pSj1 & pIC19H
RC   pSj10 from plasmid & ptac or ptac12deltaEco
RC   p4.5 from pSj5 & pMC9
RC   pSj10Bam7Stop7 from pSj10
RC   pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC   pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC   pGEX-3X from pGEX-1 & oligo, factor Xa site
RA   Smith D.B., Johnson K.S.;
RT   "Single-step purification of polypeptides expressed in Escherichia
RT   coli as fusions with glutathione S-transferase";
RL   Gene 67:31-40(1988).
XX
CC   thrombin or factor Xa protease sites to cleave protein from fusion.
CC   pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC   NM (pGEX-4T3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Pharmacia)
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(cloning directional)
CC   SE (affinity)(color yellow)
CC   PA (pGEX-2T)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-4
FT                   2. oligo, thrombin site
FT                   -> pGEX-4T-3 4968bp"
FT   misc_binding    0..0
FT                   /note="MCS unique EcoRI-SmaI-SalI-XhoI-NotI"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum
FT                   glutathione S-transferase (GST)"
FT   misc_binding    0..0
FT                   /note="SIT thrombin"
XX
SQ   Sequence 4968 BP; 1225 A; 1202 C; 1291 G; 1250 T; 0 other;
     acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg gttccgcgtg gatccccgaa ttcccgggtc gactcgagcg
     gccgcatcgt gactgactga cgatctgcct cgcgcgtttc ggtgatgacg gtgaaaacct
     ctgacacatg cagctcccgg agacggtcac agcttgtctg taagcggatg ccgggagcag
     acaagcccgt cagggcgcgt cagcgggtgt tggcgggtgt cggggcgcag ccatgaccca
     gtcacgtagc gatagcggag tgtataattc ttgaagacga aagggcctcg tgatacgcct
     atttttatag gttaatgtca tgataataat ggtttcttag acgtcaggtg gcacttttcg
     gggaaatgtg cgcggaaccc ctatttgttt atttttctaa atacattcaa atatgtatcc
     gctcatgaga caataaccct gataaatgct tcaataatat tgaaaaagga agagtatgag
     tattcaacat ttccgtgtcg cccttattcc cttttttgcg gcattttgcc ttcctgtttt
     tgctcaccca gaaacgctgg tgaaagtaaa agatgctgaa gatcagttgg gtgcacgagt
     gggttacatc gaactggatc tcaacagcgg taagatcctt gagagttttc gccccgaaga
     acgttttcca atgatgagca cttttaaagt tctgctatgt ggcgcggtat tatcccgtgt
     tgacgccggg caagagcaac tcggtcgccg catacactat tctcagaatg acttggttga
     gtactcacca gtcacagaaa agcatcttac ggatggcatg acagtaagag aattatgcag
     tgctgccata accatgagtg ataacactgc ggccaactta cttctgacaa cgatcggagg
     accgaaggag ctaaccgctt ttttgcacaa catgggggat catgtaactc gccttgatcg
     ttgggaaccg gagctgaatg aagccatacc aaacgacgag cgtgacacca cgatgcctgc
     agcaatggca acaacgttgc gcaaactatt aactggcgaa ctacttactc tagcttcccg
     gcaacaatta atagactgga tggaggcgga taaagttgca ggaccacttc tgcgctcggc
     ccttccggct ggctggttta ttgctgataa atctggagcc ggtgagcgtg ggtctcgcgg
     tatcattgca gcactggggc cagatggtaa gccctcccgt atcgtagtta tctacacgac
     ggggagtcag gcaactatgg atgaacgaaa tagacagatc gctgagatag gtgcctcact
     gattaagcat tggtaactgt cagaccaagt ttactcatat atactttaga ttgatttaaa
     acttcatttt taatttaaaa ggatctaggt gaagatcctt tttgataatc tcatgaccaa
     aatcccttaa cgtgagtttt cgttccactg agcgtcagac cccgtagaaa agatcaaagg
     atcttcttga gatccttttt ttctgcgcgt aatctgctgc ttgcaaacaa aaaaaccacc
     gctaccagcg gtggtttgtt tgccggatca agagctacca actctttttc cgaaggtaac
     tggcttcagc agagcgcaga taccaaatac tgtccttcta gtgtagccgt agttaggcca
     ccacttcaag aactctgtag caccgcctac atacctcgct ctgctaatcc tgttaccagt
     ggctgctgcc agtggcgata agtcgtgtct taccgggttg gactcaagac gatagttacc
     ggataaggcg cagcggtcgg gctgaacggg gggttcgtgc acacagccca gcttggagcg
     aacgacctac accgaactga gatacctaca gcgtgagcta tgagaaagcg ccacgcttcc
     cgaagggaga aaggcggaca ggtatccggt aagcggcagg gtcggaacag gagagcgcac
     gagggagctt ccagggggaa acgcctggta tctttatagt cctgtcgggt ttcgccacct
     ctgacttgag cgtcgatttt tgtgatgctc gtcagggggg cggagcctat ggaaaaacgc
     cagcaacgcg gcctttttac ggttcctggc cttttgctgg ccttttgctc acatgttctt
     tcctgcgtta tcccctgatt ctgtggataa ccgtattacc gcctttgagt gagctgatac
     cgctcgccgc agccgaacga ccgagcgcag cgagtcagtg agcgaggaag cggaagagcg
     cctgatgcgg tattttctcc ttacgcatct gtgcggtatt tcacaccgca taaattccga
     caccatcgaa tggtgcaaaa cctttcgcgg tatggcatga tagcgcccgg aagagagtca
     attcagggtg gtgaatgtga aaccagtaac gttatacgat gtcgcagagt atgccggtgt
     ctcttatcag accgtttccc gcgtggtgaa ccaggccagc cacgtttctg cgaaaacgcg
     ggaaaaagtg gaagcggcga tggcggagct gaattacatt cccaaccgcg tggcacaaca
     actggcgggc aaacagtcgt tgctgattgg cgttgccacc tccagtctgg ccctgcacgc
     gccgtcgcaa attgtcgcgg cgattaaatc tcgcgccgat caactgggtg ccagcgtggt
     ggtgtcgatg gtagaacgaa gcggcgtcga agcctgtaaa gcggcggtgc acaatcttct
     cgcgcaacgc gtcagtgggc tgatcattaa ctatccgctg gatgaccagg atgccattgc
     tgtggaagct gcctgcacta atgttccggc gttatttctt gatgtctctg accagacacc
     catcaacagt attattttct cccatgaaga cggtacgcga ctgggcgtgg agcatctggt
     cgcattgggt caccagcaaa tcgcgctgtt agcgggccca ttaagttctg tctcggcgcg
     tctgcgtctg gctggctggc ataaatatct cactcgcaat caaattcagc cgatagcgga
     acgggaaggc gactggagtg ccatgtccgg ttttcaacaa accatgcaaa tgctgaatga
     gggcatcgtt cccactgcga tgctggttgc caacgatcag atggcgctgg gcgcaatgcg
     cgccattacc gagtccgggc tgcgcgttgg tgcggatatc tcggtagtgg gatacgacga
     taccgaagac agctcatgtt atatcccgcc gttaaccacc atcaaacagg attttcgcct
     gctggggcaa accagcgtgg accgcttgct gcaactctct cagggccagg cggtgaaggg
     caatcagctg ttgcccgtct cactggtgaa aagaaaaacc accctggcgc ccaatacgca
     aaccgcctct ccccgcgcgt tggccgattc attaatgcag ctggcacgac aggtttcccg
     actggaaagc gggcagtgag cgcaacgcaa ttaatgtgag ttagctcact cattaggcac
     cccaggcttt acactttatg cttccggctc gtatgttgtg tggaattgtg agcggataac
     aatttcacac aggaaacagc tatgaccatg attacggatt cactggccgt cgttttacaa
     cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
     ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
     agcctgaatg gcgaatggcg ctttgcctgg tttccggcac cagaagcggt gccggaaagc
     tggctggagt gcgatcttcc tgaggccgat actgtcgtcg tcccctcaaa ctggcagatg
     cacggttacg atgcgcccat ctacaccaac gtaacctatc ccattacggt caatccgccg
     tttgttccca cggagaatcc gacgggttgt tactcgctca catttaatgt tgatgaaagc
     tggctacagg aaggccagac gcgaattatt tttgatggcg ttggaatt
//