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ID PGEX5X2 preliminary; circular DNA; SYN; 4973 BP.
XX
AC IG1078; M21676; M97937;
XX
DT 01-FEB-1993 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli plasmid vector pGEX-5X2 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC plasmid from pSj1 & pIC19H
RC pSj10 from plasmid & ptac or ptac12deltaEco
RC p4.5 from pSj5 & pMC9
RC pSj10Bam7Stop7 from pSj10
RC pGEX-1 from pBR322 & pSj10Bam7Stop7 & p4.5 & oligo
RC pGEX-2T from pGEX-1 & oligo, thrombin protease site
RC pGEX-3X from pGEX-1 & oligo, factor Xa site
RA Smith D.B., Johnson K.S.;
RT "Single-step purification of polypeptides expressed in Escherichia
RT coli as fusions with glutathione S-transferase";
RL Gene 67:31-40(1988).
XX
CC thrombin or factor Xa protease sites to cleave protein from fusion.
CC pGEX-1lambdaT, pGEX-4T-1, pGEX-5X-1 accept cDNA from lambda gt11 libs.
CC NM (pGEX-5X2)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (plasmid)
CC SP (Pharmacia)
CC HO (E.coli)
CC CP ()
CC FN (expression)(cloning directional)
CC SE (affinity)(color yellow)
CC PA (pGEX-3X)
CC BR ()
CC OF ()
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. pGEX-5 4968bp
FT 2. oligo, factor Xa site
FT -> pGEX-5X-2 4973bp"
FT misc_binding 0..0
FT /note="MCS unique BamHI-EcoRI-SmaI-SalI-XhoI-NotI"
FT CDS 0..0
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 0..0
FT /note="SIT unique PstI"
FT rep_origin 0..0
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS 0..0
FT /note="REP E. coli lacI repressor gene"
FT misc_binding 0..0
FT /note="SIT unique MluI"
FT misc_binding 0..0
FT /note="SIT unique BstEII"
FT misc_binding 0..0
FT /note="SIT unique ApaI"
FT misc_binding 0..0
FT /note="SIT unique BssHII"
FT misc_binding 0..0
FT /note="SIT unique EcoRV"
FT misc_binding 0..0
FT /note="SIT unique NarI"
FT promoter 0..0
FT /note="PRO E. coli tac (trp and lac)"
FT CDS 0..0
FT /note="GEN Schistosoma japonicum
FT glutathione S-transferase (GST)"
FT misc_binding 0..0
FT /note="SIT factor Xa"
XX
SQ Sequence 4973 BP; 1228 A; 1201 C; 1294 G; 1250 T; 0 other;
agcttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
atcctccaaa atcggatctg atcgaaggtc gtgggatccc cggaattccc gggtcgactc
gagcggccgc atcgtgactg actgacgatc tgcctcgcgc gtttcggtga tgacggtgaa
aacctctgac acatgcagct cccggagacg gtcacagctt gtctgtaagc ggatgccggg
agcagacaag cccgtcaggg cgcgtcagcg ggtgttggcg ggtgtcgggg cgcagccatg
acccagtcac gtagcgatag cggagtgtat aattcttgaa gacgaaaggg cctcgtgata
cgcctatttt tataggttaa tgtcatgata ataatggttt cttagacgtc aggtggcact
tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca ttcaaatatg
tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa aaggaagagt
atgagtattc aacatttccg tgtcgccctt attccctttt ttgcggcatt ttgccttcct
gtttttgctc acccagaaac gctggtgaaa gtaaaagatg ctgaagatca gttgggtgca
cgagtgggtt acatcgaact ggatctcaac agcggtaaga tccttgagag ttttcgcccc
gaagaacgtt ttccaatgat gagcactttt aaagttctgc tatgtggcgc ggtattatcc
cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac actattctca gaatgacttg
gttgagtact caccagtcac agaaaagcat cttacggatg gcatgacagt aagagaatta
tgcagtgctg ccataaccat gagtgataac actgcggcca acttacttct gacaacgatc
ggaggaccga aggagctaac cgcttttttg cacaacatgg gggatcatgt aactcgcctt
gatcgttggg aaccggagct gaatgaagcc ataccaaacg acgagcgtga caccacgatg
cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg gcgaactact tactctagct
tcccggcaac aattaataga ctggatggag gcggataaag ttgcaggacc acttctgcgc
tcggcccttc cggctggctg gtttattgct gataaatctg gagccggtga gcgtgggtct
cgcggtatca ttgcagcact ggggccagat ggtaagccct cccgtatcgt agttatctac
acgacgggga gtcaggcaac tatggatgaa cgaaatagac agatcgctga gataggtgcc
tcactgatta agcattggta actgtcagac caagtttact catatatact ttagattgat
ttaaaacttc atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg
accaaaatcc cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc
aaaggatctt cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa
ccaccgctac cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag
gtaactggct tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta
ggccaccact tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta
ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag
ttaccggata aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg
gagcgaacga cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg
cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag
cgcacgaggg agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc
cacctctgac ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa
aacgccagca acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg
ttctttcctg cgttatcccc tgattctgtg gataaccgta ttaccgcctt tgagtgagct
gataccgctc gccgcagccg aacgaccgag cgcagcgagt cagtgagcga ggaagcggaa
gagcgcctga tgcggtattt tctccttacg catctgtgcg gtatttcaca ccgcataaat
tccgacacca tcgaatggtg caaaaccttt cgcggtatgg catgatagcg cccggaagag
agtcaattca gggtggtgaa tgtgaaacca gtaacgttat acgatgtcgc agagtatgcc
ggtgtctctt atcagaccgt ttcccgcgtg gtgaaccagg ccagccacgt ttctgcgaaa
acgcgggaaa aagtggaagc ggcgatggcg gagctgaatt acattcccaa ccgcgtggca
caacaactgg cgggcaaaca gtcgttgctg attggcgttg ccacctccag tctggccctg
cacgcgccgt cgcaaattgt cgcggcgatt aaatctcgcg ccgatcaact gggtgccagc
gtggtggtgt cgatggtaga acgaagcggc gtcgaagcct gtaaagcggc ggtgcacaat
cttctcgcgc aacgcgtcag tgggctgatc attaactatc cgctggatga ccaggatgcc
attgctgtgg aagctgcctg cactaatgtt ccggcgttat ttcttgatgt ctctgaccag
acacccatca acagtattat tttctcccat gaagacggta cgcgactggg cgtggagcat
ctggtcgcat tgggtcacca gcaaatcgcg ctgttagcgg gcccattaag ttctgtctcg
gcgcgtctgc gtctggctgg ctggcataaa tatctcactc gcaatcaaat tcagccgata
gcggaacggg aaggcgactg gagtgccatg tccggttttc aacaaaccat gcaaatgctg
aatgagggca tcgttcccac tgcgatgctg gttgccaacg atcagatggc gctgggcgca
atgcgcgcca ttaccgagtc cgggctgcgc gttggtgcgg atatctcggt agtgggatac
gacgataccg aagacagctc atgttatatc ccgccgttaa ccaccatcaa acaggatttt
cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
aagggcaatc agctgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc acgacaggtt
tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc tcactcatta
ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
ataacaattt cacacaggaa acagctatga ccatgattac ggattcactg gccgtcgttt
tacaacgtcg tgactgggaa aaccctggcg ttacccaact taatcgcctt gcagcacatc
cccctttcgc cagctggcgt aatagcgaag aggcccgcac cgatcgccct tcccaacagt
tgcgcagcct gaatggcgaa tggcgctttg cctggtttcc ggcaccagaa gcggtgccgg
aaagctggct ggagtgcgat cttcctgagg ccgatactgt cgtcgtcccc tcaaactggc
agatgcacgg ttacgatgcg cccatctaca ccaacgtaac ctatcccatt acggtcaatc
cgccgtttgt tcccacggag aatccgacgg gttgttactc gctcacattt aatgttgatg
aaagctggct acaggaaggc cagacgcgaa ttatttttga tggcgttgga att
//