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ID   PGEXKG     preliminary; circular DNA; SYN; 5006 BP.
XX
AC   IG7000; ATCC77103;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pGEX-KG - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGEX-KG from pGEX-2T
RA   Guan K.L., Dixon J.E.;
RT   "Eukaryotic proteins expressed in Escherichia coli: an improved
RT   thrombin cleavage and purification procedure of fusion proteins
RT   with glutathione S-transferase";
RL   Anal. Biochem. 192:262-267(1991).
XX
CC   NM (pGEX-KG)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(E.coli HB101)(E.coli JM101)(E.coli TG1)(E.coli BL21)
CC   CP ()
CC   FN (expression)
CC   SE ()
CC   PA (pGEX-2T)
CC   BR (pGEX-KN)(pGEX-KT)
CC   OF (pKG-PTPU323)
CC   OR (yes)
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGEX-2T EcoRI 4948bp 941..941
FT                   2. oligo EcoRI-EcoRI 58bp, glycine/stop codons
FT                   -> pGEX-KG 5006bp"
FT   misc_binding    0..0
FT                   /note="SIT unique BspMI"
FT   promoter        0..0
FT                   /note="PRO E. coli tac (trp and lac)"
FT   CDS             0..0
FT                   /note="GEN Schistosoma japonicum
FT                   glutathione S-transferase (GST)"
FT   misc_binding    0..0
FT                   /note="SIT unique BalI"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-SmaI-EcoRI-XbaI-NcoI-SalI-
FT                   XhoI-SacI-HindIII"
FT   misc_feature    0..0
FT                   /note="oligonucleotide insert into pGEX-2T at EcoRI"
FT   misc_feature    0..0
FT                   /note="SIT glycine kinker"
FT   misc_feature    0..0
FT                   /note="stop codons"
FT   misc_binding    0..0
FT                   /note="SIT unique Tth1111"
FT   misc_binding    0..0
FT                   /note="SIT unique AatII"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique AlwNI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT unique MluI"
FT   misc_binding    0..0
FT                   /note="SIT unique BstEII"
FT   misc_binding    0..0
FT                   /note="SIT unique ApaI"
FT   misc_binding    0..0
FT                   /note="SIT unique BssHII"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRV"
FT   misc_binding    0..0
FT                   /note="SIT unique NarI"
XX
SQ   Sequence 5006 BP; 1234 A; 1205 C; 1302 G; 1265 T; 0 other;
     acgttatcga ctgcacggtg caccaatgct tctggcgtca ggcagccatc ggaagctgtg
     gtatggctgt gcaggtcgta aatcactgca taattcgtgt cgctcaaggc gcactcccgt
     tctggataat gttttttgcg ccgacatcat aacggttctg gcaaatattc tgaaatgagc
     tgttgacaat taatcatcgg ctcgtataat gtgtggaatt gtgagcggat aacaatttca
     cacaggaaac agtattcatg tcccctatac taggttattg gaaaattaag ggccttgtgc
     aacccactcg acttcttttg gaatatcttg aagaaaaata tgaagagcat ttgtatgagc
     gcgatgaagg tgataaatgg cgaaacaaaa agtttgaatt gggtttggag tttcccaatc
     ttccttatta tattgatggt gatgttaaat taacacagtc tatggccatc atacgttata
     tagctgacaa gcacaacatg ttgggtggtt gtccaaaaga gcgtgcagag atttcaatgc
     ttgaaggagc ggttttggat attagatacg gtgtttcgag aattgcatat agtaaagact
     ttgaaactct caaagttgat tttcttagca agctacctga aatgctgaaa atgttcgaag
     atcgtttatg tcataaaaca tatttaaatg gtgatcatgt aacccatcct gacttcatgt
     tgtatgacgc tcttgatgtt gttttataca tggacccaat gtgcctggat gcgttcccaa
     aattagtttg ttttaaaaaa cgtattgaag ctatcccaca aattgataag tacttgaaat
     ccagcaagta tatagcatgg cctttgcagg gctggcaagc cacgtttggt ggtggcgacc
     atcctccaaa atcggatctg gttccgcgtg gatccccggg aaatttccgg tggtggtggt
     ggaattctag actccatggg tcgactcgag ctcaagctta ttcatcgtga ctgactgacg
     atctgcctcg cgcgtttcgg tgatgacggt gaaaacctct gacacatgca gctcccggag
     acggtcacag cttgtctgta agcggatgcc gggagcagac aagcccgtca gggcgcgtca
     gcgggtgttg gcgggtgtcg gggcgcagcc atgacccagt cacgtagcga tagcggagtg
     tataattctt gaagacgaaa gggcctcgtg atacgcctat ttttataggt taatgtcatg
     ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg cggaacccct
     atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca ataaccctga
     taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt ccgtgtcgcc
     cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga aacgctggtg
     aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga actggatctc
     aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat gatgagcact
     tttaaagttc tgctatgtgg cgcggtatta tcccgtgttg acgccgggca agagcaactc
     ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt cacagaaaag
     catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac catgagtgat
     aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct aaccgctttt
     ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga gctgaatgaa
     gccataccaa acgacgagcg tgacaccacg atgcctgcag caatggcaac aacgttgcgc
     aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat agactggatg
     gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg ctggtttatt
     gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc actggggcca
     gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc aactatggat
     gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg gtaactgtca
     gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta atttaaaagg
     atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg tgagttttcg
     ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga tccttttttt
     ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt ggtttgtttg
     ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag agcgcagata
     ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa ctctgtagca
     ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag tggcgataag
     tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca gcggtcgggc
     tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac cgaactgaga
     tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa ggcggacagg
     tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc agggggaaac
     gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg tcgatttttg
     tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc ctttttacgg
     ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc ccctgattct
     gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag ccgaacgacc
     gagcgcagcg agtcagtgag cgaggaagcg gaagagcgcc tgatgcggta ttttctcctt
     acgcatctgt gcggtatttc acaccgcata aattccgaca ccatcgaatg gtgcaaaacc
     tttcgcggta tggcatgata gcgcccggaa gagagtcaat tcagggtggt gaatgtgaaa
     ccagtaacgt tatacgatgt cgcagagtat gccggtgtct cttatcagac cgtttcccgc
     gtggtgaacc aggccagcca cgtttctgcg aaaacgcggg aaaaagtgga agcggcgatg
     gcggagctga attacattcc caaccgcgtg gcacaacaac tggcgggcaa acagtcgttg
     ctgattggcg ttgccacctc cagtctggcc ctgcacgcgc cgtcgcaaat tgtcgcggcg
     attaaatctc gcgccgatca actgggtgcc agcgtggtgg tgtcgatggt agaacgaagc
     ggcgtcgaag cctgtaaagc ggcggtgcac aatcttctcg cgcaacgcgt cagtgggctg
     atcattaact atccgctgga tgaccaggat gccattgctg tggaagctgc ctgcactaat
     gttccggcgt tatttcttga tgtctctgac cagacaccca tcaacagtat tattttctcc
     catgaagacg gtacgcgact gggcgtggag catctggtcg cattgggtca ccagcaaatc
     gcgctgttag cgggcccatt aagttctgtc tcggcgcgtc tgcgtctggc tggctggcat
     aaatatctca ctcgcaatca aattcagccg atagcggaac gggaaggcga ctggagtgcc
     atgtccggtt ttcaacaaac catgcaaatg ctgaatgagg gcatcgttcc cactgcgatg
     ctggttgcca acgatcagat ggcgctgggc gcaatgcgcg ccattaccga gtccgggctg
     cgcgttggtg cggatatctc ggtagtggga tacgacgata ccgaagacag ctcatgttat
     atcccgccgt taaccaccat caaacaggat tttcgcctgc tggggcaaac cagcgtggac
     cgcttgctgc aactctctca gggccaggcg gtgaagggca atcagctgtt gcccgtctca
     ctggtgaaaa gaaaaaccac cctggcgccc aatacgcaaa ccgcctctcc ccgcgcgttg
     gccgattcat taatgcagct ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg
     caacgcaatt aatgtgagtt agctcactca ttaggcaccc caggctttac actttatgct
     tccggctcgt atgttgtgtg gaattgtgag cggataacaa tttcacacag gaaacagcta
     tgaccatgat tacggattca ctggccgtcg ttttacaacg tcgtgactgg gaaaaccctg
     gcgttaccca acttaatcgc cttgcagcac atcccccttt cgccagctgg cgtaatagcg
     aagaggcccg caccgatcgc ccttcccaac agttgcgcag cctgaatggc gaatggcgct
     ttgcctggtt tccggcacca gaagcggtgc cggaaagctg gctggagtgc gatcttcctg
     aggccgatac tgtcgtcgtc ccctcaaact ggcagatgca cggttacgat gcgcccatct
     acaccaacgt aacctatccc attacggtca atccgccgtt tgttcccacg gagaatccga
     cgggttgtta ctcgctcaca tttaatgttg atgaaagctg gctacaggaa ggccagacgc
     gaattatttt tgatggcgtt ggaatt
//