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ID   PGFP       preliminary; circular DNA; SYN; 3344 BP.
XX
AC   U17997;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid pGFP - complete.
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KW   cloning vector; Green fluorescence protein; GFP.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RP   1-3344
RC   pGFP from pBR322
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.3";
RL   Unpublished (1994).
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RN   [2]
RP   1-3344
RC   pGFP from pBR322
RA   Chalfie M., Tu Y., Euskirchen G., Ward W.W., Prasher D.C.;
RT   "Green fluorescent protein as a marker for gene expression";
RL   Science 263:802-805(1994).
XX
RN   [3]
RP   1-3344
RC   pGFP from pBR322
RA   Kitts P.A.;
RT   ;
RL   Submitted (02-DEC-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
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CC   In reference 2, this vector is called TU#60.
CC   NCBI gi: 603191
CC   NM (pGFP)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pGFP 3344bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
XX
SQ   Sequence 3344 BP; 905 A; 774 C; 822 G; 843 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
     catgcctgca ggtcgactct agaggatccc cgggtaccgg tagaaaaaat gagtaaagga
     gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga tgttaatggg
     cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa acttaccctt
     aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt cactactttc
     tcttatggtg ttcaatgctt ttcaagatac ccagatcata tgaaacggca tgactttttc
     aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa agatgacggg
     aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa tagaatcgag
     ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt ggaatacaac
     tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat caaagttaac
     ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca ttatcaacaa
     aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct gtccacacaa
     tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct tgagtttgta
     acagctgctg ggattacaca tggcatggat gaactataca aatagcattc gtagaattcc
     aactgagcgc cggtcgctac cattaccaac ttgtctggtg tcaaaaataa taggcctact
     agtcggccgt acgggccctt tcgtctcgcg cgtttcggtg atgacggtga aaacctctga
     cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg gagcagacaa
     gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gctggcttaa ctatgcggca
     tcagagcaga ttgtactgag agtgcaccat atgcggtgtg aaataccgca cagatgcgta
     aggagaaaat accgcatcag gcggccttaa gggcctcgtg atacgcctat ttttataggt
     taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaag
//