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ID   PGFP1      preliminary; circular DNA; SYN; 4146 BP.
XX
AC   U19276;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pGFP-1 - complete.
XX
KW   cloning vector; Green fluorescent protein; GFP.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4146
RC   pGFP-1 from pGFP
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.3";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4146
RC   pGFP-1 from pGFP
RA   Kitts P.A.;
RT   "Direct Submission";
RL   Submitted (28-DEC-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC   NCBI gi: 632514
CC   NM (pGFP-1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322
FT                   -> pGFP-1 4146bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             97..825
FT                   /note="GEN green fluorescent protein;
FT                   NCBI gi: 632515"
FT   CDS             2042..2836
FT                   /note="ANT E. coli neo gene;
FT                   NCBI gi: 632516"
XX
SQ   Sequence 4146 BP; 1065 A; 1038 C; 1041 G; 1002 T; 0 other;
     tagttattac tagcgctacc ggactcagat ctcgagctca agcttcgaat tctgcagtcg
     acggtaccgc gggcccggga tccaccggtc gccaccatgg gtaaaggaga agaacttttc
     actggagttg tcccaattct tgttgaatta gatggtgatg ttaatgggca caaattttct
     gtcagtggag agggtgaagg tgatgcaaca tacggaaaac ttacccttaa atttatttgc
     actactggaa aactacctgt tccatggcca acacttgtca ctactttctc ttatggtgtt
     caatgctttt caagataccc agatcatatg aaacggcatg actttttcaa gagtgccatg
     cccgaaggtt atgtacagga aagaactata tttttcaaag atgacgggaa ctacaagaca
     cgtgctgaag tcaagtttga aggtgatacc cttgttaata gaatcgagtt aaaaggtatt
     gattttaaag aagatggaaa cattcttgga cacaaattgg aatacaacta taactcacac
     aatgtataca tcatggcaga caaacaaaag aatggaatca aagttaactt caaaattaga
     cacaacattg aagatggaag cgttcaacta gcagaccatt atcaacaaaa tactccaatt
     ggcgatggcc ctgtcctttt accagacaac cattacctgt ccacacaatc tgccctttcg
     aaagatccca acgaaaagag agaccacatg gtccttcttg agtttgtaac agctgctggg
     attacacatg gcatggatga actatacaag tccggatcta gataactgat cataatcagc
     cataccacat ttgtagaggt tttacttgct ttaaaaaacc tcccacacct ccccctgaac
     ctgaaacata aaatgaatgc aattgttgtt gttaacttgt ttattgcagc ttataatggt
     tacaaataaa gcaatagcat cacaaatttc acaaataaag catttttttc actgcattct
     agttgtggtt tgtccaaact catcaatgta tcttaacgcg taaattgtaa gcgttaatat
     tttgttaaaa ttcgcgttaa atttttgtta aatcagctca ttttttaacc aataggccga
     aatcggcaaa atcccttata aatcaaaaga atagaccgag atagggttga gtgttgttcc
     agtttggaac aagagtccac tattaaagaa cgtggactcc aacgtcaaag ggcgaaaaac
     cgtctatcag ggcgatggcc cactacgtga accatcaccc taatcaagtt ttttggggtc
     gaggtgccgt aaagcactaa atcggaaccc taaagggagc ccccgattta gagcttgacg
     gggaaagccg gcgaacgtgg cgagaaagga agggaagaaa gcgaaaggag cgggcgctag
     ggcgctggca agtgtagcgg tcacgctgcg cgtaaccacc acacccgccg cgcttaatgc
     gccgctacag ggcgcgtcag gtggcacttt tcggggaaat gtgcgcggaa cccctatttg
     tttatttttc taaatacatt caaatatgta tccgctcatg agacaataac cctgataaat
     gcttcaataa tattgaaaaa ggaagagtcc tgaggcggaa agaaccagct gtggaatgtg
     tgtcagttag ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg
     catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt
     atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc
     ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt
     atttatgcag aggccgaggc cgcctcggcc tctgagctat tccagaagta gtgaggaggc
     ttttttggag gcctaggctt ttgcaaagat cgatcaagag acaggatgag gatcgtttcg
     catgattgaa caagatggat tgcacgcagg ttctccggcc gcttgggtgg agaggctatt
     cggctatgac tgggcacaac agacaatcgg ctgctctgat gccgccgtgt tccggctgtc
     agcgcagggg cgcccggttc tttttgtcaa gaccgacctg tccggtgccc tgaatgaact
     gcaagacgag gcagcgcggc tatcgtggct ggccacgacg ggcgttcctt gcgcagctgt
     gctcgacgtt gtcactgaag cgggaaggga ctggctgcta ttgggcgaag tgccggggca
     ggatctcctg tcatctcacc ttgctcctgc cgagaaagta tccatcatgg ctgatgcaat
     gcggcggctg catacgcttg atccggctac ctgcccattc gaccaccaag cgaaacatcg
     catcgagcga gcacgtactc ggatggaagc cggtcttgtc gatcaggatg atctggacga
     agagcatcag gggctcgcgc cagccgaact gttcgccagg ctcaaggcga gcatgcccga
     cggcgaggat ctcgtcgtga cccatggcga tgcctgcttg ccgaatatca tggtggaaaa
     tggccgcttt tctggattca tcgactgtgg ccggctgggt gtggcggacc gctatcagga
     catagcgttg gctacccgtg atattgctga agagcttggc ggcgaatggg ctgaccgctt
     cctcgtgctt tacggtatcg ccgctcccga ttcgcagcgc atcgccttct atcgccttct
     tgacgagttc ttctgagcgg gactctgggg ttcgaaatga ccgaccaagc gacgcccaac
     ctgccatcac gagatttcga ttccaccgcc gccttctatg aaaggttggg cttcggaatc
     gttttccggg acgccggctg gatgatcctc cagcgcgggg atctcatgct ggagttcttc
     gcccacccta gggggaggct aactgaaaca cggaaggaga caataccgga aggaacccgc
     gctatgacgg caataaaaag acagaataaa acgcacggtg ttgggtcgtt tgttcataaa
     cgcggggttc ggtcccaggg ctggcactct gtcgataccc caccgagacc ccattggggc
     caatacgccc gcgtttcttc cttttcccca ccccaccccc caagttcggg tgaaggccca
     gggctcgcag ccaacgtcgg ggcggcaggc cctgccatag cctcaggtta ctcatatata
     ctttagattg atttaaaact tcatttttaa tttaaaagga tctaggtgaa gatccttttt
     gataatctca tgaccaaaat cccttaacgt gagttttcgt tccactgagc gtcagacccc
     gtagaaaaga tcaaaggatc ttcttgagat cctttttttc tgcgcgtaat ctgctgcttg
     caaacaaaaa aaccaccgct accagcggtg gtttgtttgc cggatcaaga gctaccaact
     ctttttccga aggtaactgg cttcagcaga gcgcagatac caaatactgt ccttctagtg
     tagccgtagt taggccacca cttcaagaac tctgtagcac cgcctacata cctcgctctg
     ctaatcctgt taccagtggc tgctgccagt ggcgataagt cgtgtcttac cgggttggac
     tcaagacgat agttaccgga taaggcgcag cggtcgggct gaacgggggg ttcgtgcaca
     cagcccagct tggagcgaac gacctacacc gaactgagat acctacagcg tgagctatga
     gaaagcgcca cgcttcccga agggagaaag gcggacaggt atccggtaag cggcagggtc
     ggaacaggag agcgcacgag ggagcttcca gggggaaacg cctggtatct ttatagtcct
     gtcgggtttc gccacctctg acttgagcgt cgatttttgt gatgctcgtc aggggggcgg
     agcctatgga aaaacgccag caacgcggcc tttttacggt tcctggcctt ttgctggcct
     tttgctcaca tgttctttcc tgcgttatcc cctgattctg tggataaccg tattaccgcc
     atgcat
//