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ID   PGFPN1     preliminary; circular DNA; SYN; 4728 BP.
XX
AC   U19279;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pGFP-N1 - complete.
XX
KW   cloning vector; Green fluorescent protein; GFP.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4728
RC   pGFP-N1 from pGFP
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.3";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4728
RC   pGFP-N1 from pGFP
RA   Kitts P.A.;
RT   ;
RL   Submitted (28-DEC-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC   NCBI gi: 632523
CC   NM (pGFP-N1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pGFP-N1 4728bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             679..1407
FT                   /note="GEN green fluorescent protein;
FT                   NCBI gi: 632524"
FT   CDS             2624..3418
FT                   /note="ANT E. coli neo gene;
FT                   NCBI gi: 632525"
XX
SQ   Sequence 4728 BP; 1211 A; 1181 C; 1182 G; 1154 T; 0 other;
     tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
     ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
     gatccaccgg tcgccaccat gggtaaagga gaagaacttt tcactggagt tgtcccaatt
     cttgttgaat tagatggtga tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa
     ggtgatgcaa catacggaaa acttaccctt aaatttattt gcactactgg aaaactacct
     gttccatggc caacacttgt cactactttc tcttatggtg ttcaatgctt ttcaagatac
     ccagatcata tgaaacggca tgactttttc aagagtgcca tgcccgaagg ttatgtacag
     gaaagaacta tatttttcaa agatgacggg aactacaaga cacgtgctga agtcaagttt
     gaaggtgata cccttgttaa tagaatcgag ttaaaaggta ttgattttaa agaagatgga
     aacattcttg gacacaaatt ggaatacaac tataactcac acaatgtata catcatggca
     gacaaacaaa agaatggaat caaagttaac ttcaaaatta gacacaacat tgaagatgga
     agcgttcaac tagcagacca ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt
     ttaccagaca accattacct gtccacacaa tctgcccttt cgaaagatcc caacgaaaag
     agagaccaca tggtccttct tgagtttgta acagctgctg ggattacaca tggcatggat
     gaactataca agtccggatc tagataactg atcataatca gccataccac atttgtagag
     gttttacttg ctttaaaaaa cctcccacac ctccccctga acctgaaaca taaaatgaat
     gcaattgttg ttgttaactt gtttattgca gcttataatg gttacaaata aagcaatagc
     atcacaaatt tcacaaataa agcatttttt tcactgcatt ctagttgtgg tttgtccaaa
     ctcatcaatg tatcttaacg cgtaaattgt aagcgttaat attttgttaa aattcgcgtt
     aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt tctaaataca
     ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat aatattgaaa
     aaggaagagt cctgaggcgg aaagaaccag ctgtggaatg tgtgtcagtt agggtgtgga
     aagtccccag gctccccagc aggcagaagt atgcaaagca tgcatctcaa ttagtcagca
     accaggtgtg gaaagtcccc aggctcccca gcaggcagaa gtatgcaaag catgcatctc
     aattagtcag caaccatagt cccgccccta actccgccca tcccgcccct aactccgccc
     agttccgccc attctccgcc ccatggctga ctaatttttt ttatttatgc agaggccgag
     gccgcctcgg cctctgagct attccagaag tagtgaggag gcttttttgg aggcctaggc
     ttttgcaaag atcgatcaag agacaggatg aggatcgttt cgcatgattg aacaagatgg
     attgcacgca ggttctccgg ccgcttgggt ggagaggcta ttcggctatg actgggcaca
     acagacaatc ggctgctctg atgccgccgt gttccggctg tcagcgcagg ggcgcccggt
     tctttttgtc aagaccgacc tgtccggtgc cctgaatgaa ctgcaagacg aggcagcgcg
     gctatcgtgg ctggccacga cgggcgttcc ttgcgcagct gtgctcgacg ttgtcactga
     agcgggaagg gactggctgc tattgggcga agtgccgggg caggatctcc tgtcatctca
     ccttgctcct gccgagaaag tatccatcat ggctgatgca atgcggcggc tgcatacgct
     tgatccggct acctgcccat tcgaccacca agcgaaacat cgcatcgagc gagcacgtac
     tcggatggaa gccggtcttg tcgatcagga tgatctggac gaagagcatc aggggctcgc
     gccagccgaa ctgttcgcca ggctcaaggc gagcatgccc gacggcgagg atctcgtcgt
     gacccatggc gatgcctgct tgccgaatat catggtggaa aatggccgct tttctggatt
     catcgactgt ggccggctgg gtgtggcgga ccgctatcag gacatagcgt tggctacccg
     tgatattgct gaagagcttg gcggcgaatg ggctgaccgc ttcctcgtgc tttacggtat
     cgccgctccc gattcgcagc gcatcgcctt ctatcgcctt cttgacgagt tcttctgagc
     gggactctgg ggttcgaaat gaccgaccaa gcgacgccca acctgccatc acgagatttc
     gattccaccg ccgccttcta tgaaaggttg ggcttcggaa tcgttttccg ggacgccggc
     tggatgatcc tccagcgcgg ggatctcatg ctggagttct tcgcccaccc tagggggagg
     ctaactgaaa cacggaagga gacaataccg gaaggaaccc gcgctatgac ggcaataaaa
     agacagaata aaacgcacgg tgttgggtcg tttgttcata aacgcggggt tcggtcccag
     ggctggcact ctgtcgatac cccaccgaga ccccattggg gccaatacgc ccgcgtttct
     tccttttccc caccccaccc cccaagttcg ggtgaaggcc cagggctcgc agccaacgtc
     ggggcggcag gccctgccat agcctcaggt tactcatata tactttagat tgatttaaaa
     cttcattttt aatttaaaag gatctaggtg aagatccttt ttgataatct catgaccaaa
     atcccttaac gtgagttttc gttccactga gcgtcagacc ccgtagaaaa gatcaaagga
     tcttcttgag atcctttttt tctgcgcgta atctgctgct tgcaaacaaa aaaaccaccg
     ctaccagcgg tggtttgttt gccggatcaa gagctaccaa ctctttttcc gaaggtaact
     ggcttcagca gagcgcagat accaaatact gtccttctag tgtagccgta gttaggccac
     cacttcaaga actctgtagc accgcctaca tacctcgctc tgctaatcct gttaccagtg
     gctgctgcca gtggcgataa gtcgtgtctt accgggttgg actcaagacg atagttaccg
     gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca cacagcccag cttggagcga
     acgacctaca ccgaactgag atacctacag cgtgagctat gagaaagcgc cacgcttccc
     gaagggagaa aggcggacag gtatccggta agcggcaggg tcggaacagg agagcgcacg
     agggagcttc cagggggaaa cgcctggtat ctttatagtc ctgtcgggtt tcgccacctc
     tgacttgagc gtcgattttt gtgatgctcg tcaggggggc ggagcctatg gaaaaacgcc
     agcaacgcgg cctttttacg gttcctggcc ttttgctggc cttttgctca catgttcttt
     cctgcgttat cccctgattc tgtggataac cgtattaccg ccatgcat
//