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ID   PGFPN2     preliminary; circular DNA; SYN; 4732 BP.
XX
AC   U19282;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pGFP-N2 - complete.
XX
KW   cloning vector; Green fluorescent protein; GFP.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4732
RC   pGFP-N2 from pGFP
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.3";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4732
RC   pGFP-N2 from pGFP
RA   Kitts P.A.;
RT   ;
RL   Submitted (28-DEC-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC   NCBI gi: 632532
CC   NM (pGFP-N2)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pGFP-N2 4732bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             683..1411
FT                   /note="GEN green fluorescent protein;
FT                   NCBI gi: 632533"
FT   CDS             2628..3422
FT                   /note="ANT E. coli neo gene;
FT                   NCBI gi: 632534"
XX
SQ   Sequence 4732 BP; 1211 A; 1183 C; 1184 G; 1154 T; 0 other;
     tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
     ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
     gatccaccgg ccggtcgcca ccatgggtaa aggagaagaa cttttcactg gagttgtccc
     aattcttgtt gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg
     tgaaggtgat gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact
     acctgttcca tggccaacac ttgtcactac tttctcttat ggtgttcaat gcttttcaag
     atacccagat catatgaaac ggcatgactt tttcaagagt gccatgcccg aaggttatgt
     acaggaaaga actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa
     gtttgaaggt gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga
     tggaaacatt cttggacaca aattggaata caactataac tcacacaatg tatacatcat
     ggcagacaaa caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga
     tggaagcgtt caactagcag accattatca acaaaatact ccaattggcg atggccctgt
     ccttttacca gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga
     aaagagagac cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat
     ggatgaacta tacaagtccg gatctagata actgatcata atcagccata ccacatttgt
     agaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     caaactcatc aatgtatctt aacgcgtaaa ttgtaagcgt taatattttg ttaaaattcg
     cgttaaattt ttgttaaatc agctcatttt ttaaccaata ggccgaaatc ggcaaaatcc
     cttataaatc aaaagaatag accgagatag ggttgagtgt tgttccagtt tggaacaaga
     gtccactatt aaagaacgtg gactccaacg tcaaagggcg aaaaaccgtc tatcagggcg
     atggcccact acgtgaacca tcaccctaat caagtttttt ggggtcgagg tgccgtaaag
     cactaaatcg gaaccctaaa gggagccccc gatttagagc ttgacgggga aagccggcga
     acgtggcgag aaaggaaggg aagaaagcga aaggagcggg cgctagggcg ctggcaagtg
     tagcggtcac gctgcgcgta accaccacac ccgccgcgct taatgcgccg ctacagggcg
     cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     gaaaaaggaa gagtcctgag gcggaaagaa ccagctgtgg aatgtgtgtc agttagggtg
     tggaaagtcc ccaggctccc cagcaggcag aagtatgcaa agcatgcatc tcaattagtc
     agcaaccagg tgtggaaagt ccccaggctc cccagcaggc agaagtatgc aaagcatgca
     tctcaattag tcagcaacca tagtcccgcc cctaactccg cccatcccgc ccctaactcc
     gcccagttcc gcccattctc cgccccatgg ctgactaatt ttttttattt atgcagaggc
     cgaggccgcc tcggcctctg agctattcca gaagtagtga ggaggctttt ttggaggcct
     aggcttttgc aaagatcgat caagagacag gatgaggatc gtttcgcatg attgaacaag
     atggattgca cgcaggttct ccggccgctt gggtggagag gctattcggc tatgactggg
     cacaacagac aatcggctgc tctgatgccg ccgtgttccg gctgtcagcg caggggcgcc
     cggttctttt tgtcaagacc gacctgtccg gtgccctgaa tgaactgcaa gacgaggcag
     cgcggctatc gtggctggcc acgacgggcg ttccttgcgc agctgtgctc gacgttgtca
     ctgaagcggg aagggactgg ctgctattgg gcgaagtgcc ggggcaggat ctcctgtcat
     ctcaccttgc tcctgccgag aaagtatcca tcatggctga tgcaatgcgg cggctgcata
     cgcttgatcc ggctacctgc ccattcgacc accaagcgaa acatcgcatc gagcgagcac
     gtactcggat ggaagccggt cttgtcgatc aggatgatct ggacgaagag catcaggggc
     tcgcgccagc cgaactgttc gccaggctca aggcgagcat gcccgacggc gaggatctcg
     tcgtgaccca tggcgatgcc tgcttgccga atatcatggt ggaaaatggc cgcttttctg
     gattcatcga ctgtggccgg ctgggtgtgg cggaccgcta tcaggacata gcgttggcta
     cccgtgatat tgctgaagag cttggcggcg aatgggctga ccgcttcctc gtgctttacg
     gtatcgccgc tcccgattcg cagcgcatcg ccttctatcg ccttcttgac gagttcttct
     gagcgggact ctggggttcg aaatgaccga ccaagcgacg cccaacctgc catcacgaga
     tttcgattcc accgccgcct tctatgaaag gttgggcttc ggaatcgttt tccgggacgc
     cggctggatg atcctccagc gcggggatct catgctggag ttcttcgccc accctagggg
     gaggctaact gaaacacgga aggagacaat accggaagga acccgcgcta tgacggcaat
     aaaaagacag aataaaacgc acggtgttgg gtcgtttgtt cataaacgcg gggttcggtc
     ccagggctgg cactctgtcg ataccccacc gagaccccat tggggccaat acgcccgcgt
     ttcttccttt tccccacccc accccccaag ttcgggtgaa ggcccagggc tcgcagccaa
     cgtcggggcg gcaggccctg ccatagcctc aggttactca tatatacttt agattgattt
     aaaacttcat ttttaattta aaaggatcta ggtgaagatc ctttttgata atctcatgac
     caaaatccct taacgtgagt tttcgttcca ctgagcgtca gaccccgtag aaaagatcaa
     aggatcttct tgagatcctt tttttctgcg cgtaatctgc tgcttgcaaa caaaaaaacc
     accgctacca gcggtggttt gtttgccgga tcaagagcta ccaactcttt ttccgaaggt
     aactggcttc agcagagcgc agataccaaa tactgtcctt ctagtgtagc cgtagttagg
     ccaccacttc aagaactctg tagcaccgcc tacatacctc gctctgctaa tcctgttacc
     agtggctgct gccagtggcg ataagtcgtg tcttaccggg ttggactcaa gacgatagtt
     accggataag gcgcagcggt cgggctgaac ggggggttcg tgcacacagc ccagcttgga
     gcgaacgacc tacaccgaac tgagatacct acagcgtgag ctatgagaaa gcgccacgct
     tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc agggtcggaa caggagagcg
     cacgagggag cttccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg gggcggagcc tatggaaaaa
     cgccagcaac gcggcctttt tacggttcct ggccttttgc tggccttttg ctcacatgtt
     ctttcctgcg ttatcccctg attctgtgga taaccgtatt accgccatgc at
//