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ID   PGFPN3     preliminary; circular DNA; SYN; 4724 BP.
XX
AC   U19277;
XX
DT   01-FEB-1995 (Rel. 1, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid pGFP-N3 - complete.
XX
KW   cloning vector; Green fluorescent protein; GFP.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4724
RC   pGFP-N3 from pGFP
RA   Kitts P.A.;
RT   "CLONTECH Vectors On Disc version 1.3";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4724
RC   pGFP-N3 from pGFP
RA   Kitts P.A.;
RT   ;
RL   Submitted (28-DEC-1994) by:
RL   Kitts P.A., CLONTECH Laboratories, Inc.,
RL   4030 Fabian Way, Palo Alto, CA 94303, USA.
XX
CC   NCBI gi: 632517
CC   NM (pGFP-N3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp
FT                   -> pGFP-N3 4724bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             675..1403
FT                   /note="GEN green fluorescent protein;
FT                   NCBI gi: 632518"
FT   CDS             2620..3414
FT                   /note="ANT E. coli neo gene;
FT                   NCBI gi: 632519"
XX
SQ   Sequence 4724 BP; 1211 A; 1179 C; 1180 G; 1154 T; 0 other;
     tagttattaa tagtaatcaa ttacggggtc attagttcat agcccatata tggagttccg
     cgttacataa cttacggtaa atggcccgcc tggctgaccg cccaacgacc cccgcccatt
     gacgtcaata atgacgtatg ttcccatagt aacgccaata gggactttcc attgacgtca
     atgggtggag tatttacggt aaactgccca cttggcagta catcaagtgt atcatatgcc
     aagtacgccc cctattgacg tcaatgacgg taaatggccc gcctggcatt atgcccagta
     catgacctta tgggactttc ctacttggca gtacatctac gtattagtca tcgctattac
     catggtgatg cggttttggc agtacatcaa tgggcgtgga tagcggtttg actcacgggg
     atttccaagt ctccacccca ttgacgtcaa tgggagtttg ttttggcacc aaaatcaacg
     ggactttcca aaatgtcgta acaactccgc cccattgacg caaatgggcg gtaggcgtgt
     acggtgggag gtctatataa gcagagctgg tttagtgaac cgtcagatcc gctagcgcta
     ccggactcag atctcgagct caagcttcga attctgcagt cgacggtacc gcgggcccgg
     gatccatcgc caccatgggt aaaggagaag aacttttcac tggagttgtc ccaattcttg
     ttgaattaga tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg
     atgcaacata cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc
     catggccaac acttgtcact actttctctt atggtgttca atgcttttca agatacccag
     atcatatgaa acggcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa
     gaactatatt tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag
     gtgataccct tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca
     ttcttggaca caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca
     aacaaaagaa tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg
     ttcaactagc agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac
     cagacaacca ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag
     accacatggt ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac
     tatacaagtc cggatctaga taactgatca taatcagcca taccacattt gtagaggttt
     tacttgcttt aaaaaacctc ccacacctcc ccctgaacct gaaacataaa atgaatgcaa
     ttgttgttgt taacttgttt attgcagctt ataatggtta caaataaagc aatagcatca
     caaatttcac aaataaagca tttttttcac tgcattctag ttgtggtttg tccaaactca
     tcaatgtatc ttaacgcgta aattgtaagc gttaatattt tgttaaaatt cgcgttaaat
     ttttgttaaa tcagctcatt ttttaaccaa taggccgaaa tcggcaaaat cccttataaa
     tcaaaagaat agaccgagat agggttgagt gttgttccag tttggaacaa gagtccacta
     ttaaagaacg tggactccaa cgtcaaaggg cgaaaaaccg tctatcaggg cgatggccca
     ctacgtgaac catcacccta atcaagtttt ttggggtcga ggtgccgtaa agcactaaat
     cggaacccta aagggagccc ccgatttaga gcttgacggg gaaagccggc gaacgtggcg
     agaaaggaag ggaagaaagc gaaaggagcg ggcgctaggg cgctggcaag tgtagcggtc
     acgctgcgcg taaccaccac acccgccgcg cttaatgcgc cgctacaggg cgcgtcaggt
     ggcacttttc ggggaaatgt gcgcggaacc cctatttgtt tatttttcta aatacattca
     aatatgtatc cgctcatgag acaataaccc tgataaatgc ttcaataata ttgaaaaagg
     aagagtcctg aggcggaaag aaccagctgt ggaatgtgtg tcagttaggg tgtggaaagt
     ccccaggctc cccagcaggc agaagtatgc aaagcatgca tctcaattag tcagcaacca
     ggtgtggaaa gtccccaggc tccccagcag gcagaagtat gcaaagcatg catctcaatt
     agtcagcaac catagtcccg cccctaactc cgcccatccc gcccctaact ccgcccagtt
     ccgcccattc tccgccccat ggctgactaa ttttttttat ttatgcagag gccgaggccg
     cctcggcctc tgagctattc cagaagtagt gaggaggctt ttttggaggc ctaggctttt
     gcaaagatcg atcaagagac aggatgagga tcgtttcgca tgattgaaca agatggattg
     cacgcaggtt ctccggccgc ttgggtggag aggctattcg gctatgactg ggcacaacag
     acaatcggct gctctgatgc cgccgtgttc cggctgtcag cgcaggggcg cccggttctt
     tttgtcaaga ccgacctgtc cggtgccctg aatgaactgc aagacgaggc agcgcggcta
     tcgtggctgg ccacgacggg cgttccttgc gcagctgtgc tcgacgttgt cactgaagcg
     ggaagggact ggctgctatt gggcgaagtg ccggggcagg atctcctgtc atctcacctt
     gctcctgccg agaaagtatc catcatggct gatgcaatgc ggcggctgca tacgcttgat
     ccggctacct gcccattcga ccaccaagcg aaacatcgca tcgagcgagc acgtactcgg
     atggaagccg gtcttgtcga tcaggatgat ctggacgaag agcatcaggg gctcgcgcca
     gccgaactgt tcgccaggct caaggcgagc atgcccgacg gcgaggatct cgtcgtgacc
     catggcgatg cctgcttgcc gaatatcatg gtggaaaatg gccgcttttc tggattcatc
     gactgtggcc ggctgggtgt ggcggaccgc tatcaggaca tagcgttggc tacccgtgat
     attgctgaag agcttggcgg cgaatgggct gaccgcttcc tcgtgcttta cggtatcgcc
     gctcccgatt cgcagcgcat cgccttctat cgccttcttg acgagttctt ctgagcggga
     ctctggggtt cgaaatgacc gaccaagcga cgcccaacct gccatcacga gatttcgatt
     ccaccgccgc cttctatgaa aggttgggct tcggaatcgt tttccgggac gccggctgga
     tgatcctcca gcgcggggat ctcatgctgg agttcttcgc ccaccctagg gggaggctaa
     ctgaaacacg gaaggagaca ataccggaag gaacccgcgc tatgacggca ataaaaagac
     agaataaaac gcacggtgtt gggtcgtttg ttcataaacg cggggttcgg tcccagggct
     ggcactctgt cgatacccca ccgagacccc attggggcca atacgcccgc gtttcttcct
     tttccccacc ccacccccca agttcgggtg aaggcccagg gctcgcagcc aacgtcgggg
     cggcaggccc tgccatagcc tcaggttact catatatact ttagattgat ttaaaacttc
     atttttaatt taaaaggatc taggtgaaga tcctttttga taatctcatg accaaaatcc
     cttaacgtga gttttcgttc cactgagcgt cagaccccgt agaaaagatc aaaggatctt
     cttgagatcc tttttttctg cgcgtaatct gctgcttgca aacaaaaaaa ccaccgctac
     cagcggtggt ttgtttgccg gatcaagagc taccaactct ttttccgaag gtaactggct
     tcagcagagc gcagatacca aatactgtcc ttctagtgta gccgtagtta ggccaccact
     tcaagaactc tgtagcaccg cctacatacc tcgctctgct aatcctgtta ccagtggctg
     ctgccagtgg cgataagtcg tgtcttaccg ggttggactc aagacgatag ttaccggata
     aggcgcagcg gtcgggctga acggggggtt cgtgcacaca gcccagcttg gagcgaacga
     cctacaccga actgagatac ctacagcgtg agctatgaga aagcgccacg cttcccgaag
     ggagaaaggc ggacaggtat ccggtaagcg gcagggtcgg aacaggagag cgcacgaggg
     agcttccagg gggaaacgcc tggtatcttt atagtcctgt cgggtttcgc cacctctgac
     ttgagcgtcg atttttgtga tgctcgtcag gggggcggag cctatggaaa aacgccagca
     acgcggcctt tttacggttc ctggcctttt gctggccttt tgctcacatg ttctttcctg
     cgttatcccc tgattctgtg gataaccgta ttaccgccat gcat
//