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ID   PGHOST4    preliminary; circular DNA; SYN; 3794 BP.
XX
AC   IG0351;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   Broad host range/E.coli plasmid vector pGhost4 - complete.
XX
KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGhost4 from pGK series
RA   Maguin E., Duwat P., Hege T., Ehrlich D., Gruss A.;
RT   "New thermosensitive plasmid for gram-positive bacteria";
RL   J. Bacteriol. 174:5633-5638(1992).
XX
RN   [2]
RC   [from none]
RA   Biswas I., Gruss A., Ehrlich S.D., Maguin E.;
RT   "High-efficiency gene inactivation and replacement system for
RT   gram-positive bacteria";
RL   J. Bacteriol. 175:3628-3635(1993).
XX
RN   [3]
RC   from pC194
RC   from pE194
RA   Gruss A., Ehrlich S.D.;
RT   "The family of highly interrelated single-stranded deoxyribonucleic
RT   acid plasmids";
RL   Microbiological Reviews 53:231-241(1989).
XX
RN   [4]
RC   pT713rep4 from pT713 & pWV01
RC   pOR13 from pGK1 & pWV01
RA   Leenhouts K., Tolner B., Bron S., Kok J., Venema G.,
RA   Seegers J.F.M.L.;
RT   "Nucleotide sequence and characterization of the broad-host-range
RT   Lactococcal plasmid pWVO1";
RL   Plasmid 26:55-66(1991).
XX
RN   [5]
RC   pVA797, pTRK13 from Enterococcus
RC   from pGK12
RC   from pAMbeta1
RC   from pC194
RC   from pGB354
RC   from pGKV1
RC   from pSA3
RC   from pTRK13
RC   from pTV1
RC   from pVA797
RA   Luchansky J., Muriana P.M., Klaenhammer R.;
RT   "Application of electroporation for transfer of plasmid DNA to
RT   Lactobacillus, Lactococcus, Leuconostoc, Listeria, Pediococcus,
RT   Bacillus, Staphylococcus, Enterococcus, and Propionibacterium";
RL   Mol. Microbiol. 2:637-646(1988).
XX
RN   [6]
RC   from pSC101
RA   Hamilton C.H., Aldea M., Washburn B.K., Babitzke P., Kushner S.R.;
RT   "New method for generating deletions and gene replacements in
RT   Escherichia coli";
RL   J. Bacteriol. 171:4617-4622(1989).
XX
CC   NM (pGhost4)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Appligene)
CC   HO (E.coli)(Bacillus)(Clostridia)(Enterococcus)
CC   HO (Lactobacillus)(Lactococcus)(Leuconostoc)
CC   HO (Listeria)(Pediococcus)(Staphylococcus)
CC   HO (Streptococcus)(E.faecalis)(Gram-positive bacteria)
CC   CP ()
CC   FN (homologous chromosomal integration)(mutagenesis)(shuttle)
CC   FN (thermosensitive)(broad host range)(delivery vector)
CC   FN (cloning up to 7000 bp)
CC   SE ()
CC   PA (pGK12 Ts mutant)
CC   BR ()
CC   OF (pGhost5)(pGhost6)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGK series
FT                   -> pGhost4 3794bp"
FT   misc_binding    0..0
FT                   /note="SIT non-unique SacI"
FT   CDS             0..0
FT                   /note="ANT Streptococcus erythromycin resistance gene
FT                   (ery)"
FT   misc_binding    0..0
FT                   /note="SIT non-unique SacI"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 reverse"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T3"
FT   misc_binding    0..0
FT                   /note="MCS unique BstXI-SacII-NotI-XmaIII-XbaI-SpeI-
FT                   BamHI-SmaI-PstI-EcoRI-EcoRV-HindIII-ClaI-AccI-SalI-
FT                   XhoI-ApaI-DraIII-KpnI"
FT   primer_bind     0..0
FT                   /note="PRI SK"
FT   primer_bind     complement(0..0)
FT                   /note="PRI KS"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T7"
FT   primer_bind     complement(0..0)
FT                   /note="PRI bacteriophage M13 -20"
FT   rep_origin      complement(0..0)
FT                   /note="ORI Gram positive bacteria pGK12 Ts"
XX
SQ   Sequence 3794 BP; 1249 A; 552 C; 742 G; 1249 T; 2 other;
     cgattcacaa aaaataggca cacgaaaaac aagttaaggg atgcagttta tgcatccctt
     aacttactta ttaaataatt tatagctatt gaaaagagat aagaattgtt caaagctaat
     attgtttaaa tcgtcaattc ctgcatgttt taaggaattg ttaaattgat tttttgtaaa
     tattttcttg tattctttgt taacccattt cataacgaaa taattatact tttgtttatc
     tttgtgtgat attcttgatt tttttctact taatctgata agtgagctat tcactttagg
     tttaggatga aaatattctc ttggaaccat acttaatata gaaatatcaa cttctgccat
     taaaagtaat gccaatgagc gttttgtatt taataatctt ttagcaaacc cgtattccac
     gattaaataa atctcattag ctatactatc aaaaacaatt ttgcgtatta tatccgtact
     tatgttataa ggtatattac catatatttt ataggattgg tttttaggaa atttaaactg
     caatatatcc ttgtttaaaa cttggaaatt atcgtgatca acaagtttat tttctgtagt
     tttgcataat ttatggtcta tttcaatggc agttacgaaa ttacacctct ttactaattc
     aagggtaaaa tggccttttc ctgagccgat ttcaaagata ttatcatgtt catttaatct
     tatatttgtc attattttat ctatattatg ttttgaagta ataaagtttt gactgtgttt
     tatatttttc tcgttcatta taaccctctt taatttggtt atatgaattt tgcttattaa
     cgattcatta taaccactta ttttttgttt ggttgataat gaactgtgct gattacaaaa
     atactaaaaa tgcccatatt ttttcctcct tataaaatta gtataattat agcacgagct
     ctgataaata tgaacatgat gagtgatcgt taaatttata ctgcaatcgg atgcgattat
     tgaataaaag atatgagaga tttatctaat ttcttttttc ttgtaaaaaa agaaagttct
     taaaggtttt atagttttgg tcgtagagca cacggtttaa cgacttaatt acgaagtaaa
     taagtctagt gtgttagact ttatgaaatc tatatacgtt tatatatatt tattatcgca
     ttttttatta aaacgtctca aaatcgtttc tgagacgttt tagcgtttat ttcgtttagt
     tatcggcata atcgttaaaa caggcgttat cgtagcgtaa aagcccttga gcgtagcgtg
     gctttgcagc gaagatgttg tctgttagat tatgaaagcc gatgactgaa tgaaataata
     agcgcagcgc ccttctattt cggttggagg aggctcaagg gagtatgagg gaatgaaatt
     ccctcatggg tttgatttta aaaattgctt gcaattttgc cgagcggtag cgctggaaaa
     tttttgaaaa aaatttggaa tttggaaaaa aatgggggga aaggaagcga attttgcttc
     cgtactacga ccccccatta agtgccgagt gccaattttt gtgccaaaaa cgctctatcc
     caactggctc aagggtttaa ggggtttttc aatcgccaac gaatcgccaa cgttttcgcc
     aacgtttttt ataaatctat atttaagtag ctttattgtt gtttttatga ttacaaagtg
     atacactaac tttataaaat tatttgattg gagtttttta aatggtgatt tcagaatcga
     aaaaaagagt tatgatttct ctgacaaaag agcaagataa aaaattaaca gatatggcga
     aacaaaaagg tttttcaaaa tctgcggttg cggcgttagc tatagaagaa tatgcaagaa
     aggaatcaga acaaaaaaaa taagcgaaag ctcgcgtttt tagaaggata cgagttttcg
     ctacttgttt ttgataaggt aattatatca tggctattaa aaatactaaa gctagaaatt
     ttggattttt attatatcct gactcaattc ctaatgattg gaaagaaaaa ttagagagtt
     tgggcgtatc tatggctgtc agtcctttac acgatatgga cgaaaaaaaa gataaagata
     catggaataa tagtaatatt atacaaaatg gaaagcacta taaaaaacca cactatcacg
     ttatatatat tgcacgaaat cctgtaacaa tagaaagcgt taggaacaag attaagcgaa
     aattggggaa tagttcagtt gctcatgttg agatacttga ttatatcaaa ggttcatatg
     aatatttgac tcatgaatca aaggacgcta ttgctaagaa taaacatata tacgacaaaa
     aagatatttt gaacattaat gattttgata ttgaccgcta tataacactt gatgaaagcc
     aaaaaagaga attgaagaat ttacttttag atatagtgga tgactataat ttggtaaata
     caaaagattt aatggctttt attcgcctta ggggagcgga gtttggaatt ttaaatacga
     atgatgtaaa agatattgtt tcaacaaact ctagcgcctt tagattatgg tttgagggca
     attatcagtg tggatataga gcaagttatg caaaggttct tgatgctgaa acgggggaaa
     taaaatgaca aacaaagaaa aagagttatt tgctgaaaat gaggaattaa aaaaagaaat
     taaggactta aaagagcgta ttgaaagata cagagaaatg gaagttgaat taagtacaac
     aatagattta ttgagaggag ggattattga ataaataaaa gccccctgac gaaagtcgaa
     gggggttttt attttggttt gatgttgcga ttaatagcaa tacaattgca ataaacaaaa
     tgatcttcct tcaggttatg accatctgtg ccagttcgta atgtctggtc aactttccga
     ctctgagaaa cttctggaat cgctagagaa tttctggaat gggattcagg agtggacaga
     acgacacgga tatatagtgg atgtgtcaaa acgcatacca ttttgaacga tgacctctaa
     taattgttaa tcatgttggt tacgtattta ttaacttctc ctagtattag taattatcat
     ggctgtcatg gcgcattaac ggaataaagg gtgtgcttaa atcgggccat tttgcgtaat
     aagaaaaagg attaattatg agcgaattga attaataata aggtaataga tttacattag
     aaaatgaaag gggattttat gcgtgagaat gttacagtct atccctggcg aaagggggat
     gtgctgcaag gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa
     cgacggccag tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc
     ccctcgaggt cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca
     ctagttctag agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg
     ttaattgcgc gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg
     ctcacaattc cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa
     tgagtgagct aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac
     ctgtcgtgcc agnn
//