back Return to this vector's summary.
ID   PGHOST5    preliminary; circular DNA; SYN; 5249 BP.
XX
AC   IG0352;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pGhost5 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGhost5 from pGK series
RA   Maguin E.;
RT   ;
RL   Unpublished (1992).
XX
CC   NM (pGhost5)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Appligene)
CC   HO (E.coli)(Bacillus)(Clostridia)(Enterococcus)
CC   HO (Lactobacillus)(Lactococcus)(Leuconostoc)
CC   HO (Listeria)(Pediococcus)(Staphylococcus)
CC   HO (Streptococcus)(E.faecalis)(Gram-positive bacteria)
CC   CP ()
CC   FN (homologous chromosomal integration)(mutagenesis)(shuttle)
CC   FN (thermosensitive)(broad host range)(delivery vector)
CC   FN (cloning up to 7000 bp)
CC   SE ()
CC   PA (pGhost4)
CC   BR (pGhost6)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGK series
FT                   -> pGhost5 5249bp"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 -20"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS unique KpnI-XhoI-SalI-ClaI-HindIII-EcoRV-
FT                   EcoRI-PstI-SmaI-BamHI-SpeI-XbaI-XmaIII-NotI-SacII-
FT                   BstXI"
FT   primer_bind     0..0
FT                   /note="PRI KS"
FT   primer_bind     complement(0..0)
FT                   /note="PRI SK"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T3"
FT   primer_bind     complement(0..0)
FT                   /note="PRI bacteriophage M13 reverse"
FT   CDS             0..0
FT                   /note="REP E. coli ROP gene"
FT   rep_origin      complement(0..0)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    0..0
FT                   /note="SIT non-unique AflIII"
FT   CDS             complement(0..0)
FT                   /note="ANT Streptococcus erythromycin resistance gene
FT                   (ery)"
FT   misc_binding    0..0
FT                   /note="SIT non-unique SacI"
FT   rep_origin      0..0
FT                   /note="ORI Gram positive bacteria pGK12 Ts"
XX
SQ   Sequence 5249 BP; 1569 A; 973 C; 1133 G; 1559 T; 15 other;
     aggcacacga aaaacaagtt aagggatgca gtttatcggg cagcgttggg tcctggccac
     gggtgcgcat gatcgtgctc ctgtcgttga ggacccggct aggctggcgg ggttgcctta
     ctggttagca gaatgaatca ccgatacgcg agcgaacgtg aagcgactgc tgctgcaaaa
     cgtctgcgac ctgagcaaca acatgaatgg tcttcggttt ccgtgtttcg taaagtctgg
     aaacgcggaa gtcagcgccc tgcaccatta tgttccggat ctgcatcgca ggatgctgct
     ggctaccctg tggaacacct acatctgtat taacgaagcg ctggcattga ccctgagtga
     tttttctctg gtcccgccgc atccataccg ccagttgttt accctcacaa cgttccagta
     accgggcatg ttcatcatca gtaacccgta tcgtgagcat cctctctcgt ttcatcggta
     tcattacccc catgaacaga aatccccctt acacggaggc atcagtgacc aaacaggaaa
     aaaccgccct taacatggcc cgctttatca gaagccagac attaacgctt ctggagaaac
     tcaacgagct ggacgcggat gaacaggcag acatctgtga atcgcttcac gaccacgctg
     atgagcttta ccgcagctgc ctcgcgcgtt tcggtgatga cggtgaaaac ctctgacaca
     tgcagctccc ggagacggtc acagcttgtc tgtaagcgga tgccgggagc agacaagccc
     gtcagggcgc gtcagcgggt gttggcgggt gtcggggcgc agccatgacc cagtcacgta
     gcgatagcgg agtgtatact ggcttaacta tgcggcatca gagcagattg tactgagagt
     gcaccatatg cggtgtgaaa taccgcacag atgcgtaagg agaaaatacc gcatcaggcg
     ctcttccgct tcctcgctca ctgactcgct gcgctcggtc gttcggctgc ggcgagcggt
     atcagctcac tcaaaggcgg taatacggtt atccacagaa tcaggggata acgcaggaaa
     gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt aaaaaggccg cgttgctggc
     gtttttccat aggctccgcc cccctgacga gcatcacaaa aatcgacgct caagtcagag
     gtggcgaaac ccgacaggac tataaagata ccaggcgttt ccccctggaa gctccctcgt
     gcgctctcct gttccgaccc tgccgcttac cggatacctg tccgcctttc tcccttcggg
     aagcgtggcg ctttctcata gctcacgctg taggtatctc agttcggtgt aggtcgttcg
     ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc gaccgctgcg ccttatccgg
     taactatcgt cttgagtcca acccggtaag acacgactta tcgccactgg cagcagtccc
     ttaacttact tattaaataa tttatagcta ttgaaaagag ataagaattg ttcaaagcta
     atattgttta aatcgtcaat tcctgcatgt tttaaggaat tgttaaattg attttttgta
     aatattttct tgtattcttt gttaacccat ttcataacga aataattata cttttgttta
     tctttgtgtg atattcttga tttttttcta cttaatctga taagtgagct attcacttta
     ggtttaggat gaaaatattc tcttggaacc atacttaata tagaaatatc aacttctgcc
     attaaaagta atgccaatga gcgttttgta tttaataatc ttttagcaaa cccgtattcc
     acgattaaat aaatctcatt agctatacta tcaaaaacaa ttttgcgtat tatatccgta
     cttatgttat aaggtatatt accatatatt ttataggatt ggtttttagg aaatttaaac
     tgcaatatat ccttgtttaa aacttggaaa ttatcgtgat caacaagttt attttctgta
     gttttgcata atttatggtc tatttcaatg gcagttacga aattacacct ctttactaat
     tcaagggtaa aatggccttt tcctgagccg atttcaaaga tattatcatg ttcatttaat
     cttatatttg tcattatttt atctatatta tgttttgaag taataaagtt ttgactgtgt
     tttatatttt tctcgttcat tataaccctc tttaatttgg ttatatgaat tttgcttatt
     aacgattcat tataaccact tattttttgt ttggttgata atgaactgtg ctgattacaa
     aaatactaaa aatgcccata ttttttcctc cttataaaat tagtataatt atagcacgag
     ctctgataaa tatgaacatg atgagtgatc gttaaattta tactgcaatc ggatgcgatt
     attgaataaa agatatgaga gatttatcta atttcttttt tcttgtaaaa aaagaaagtt
     cttaaaggtt ttatagtttt ggtcgtagag cacacggttt aacgacttaa ttacgaagta
     aataagtcta gtgtgttaga ctttatgaaa tctatatacg tttatatata tttattatcc
     gattttttat taaaacgtct caaaatcgtt tctgagacgt tttagcgttt atttcgttta
     gttatcggca taatcgttaa aacaggcgtt atcgtagcgt aaaagccctt gagcgtagcg
     tggctttgca gcgaagatgt tgtctgttag attatgaaag ccgatgactg aatgaaataa
     taagcgcagc gcccttctat ttcggttgga ggaggctcaa gggagtatga gggaatgaaa
     ttccctcatg ggtttgattt taaaaattgc ttgcaatttt gccgagcggt agcgctggaa
     aatttttgaa aaaaatttgg aatttggaaa aaaatggggg gaaaggaagc gaattttgct
     tccgtactac gaccccccat taagtgccga gtgccaattt ttgtgccaaa aacgctctat
     cccaactggc tcaagggttt aaggggtttt tcaatcgcca acgaatcgcc aacgttttcg
     ccaacgtttt ttataaatct atatttaagt agctttattg ttgtttttat gattacaaag
     tgatacacta actttataaa attatttgat tggagttttt taaatggtga tttcagaatc
     gaaaaaaaga gttatgattt ctctgacaaa agagcaagat aaaaaattaa cagatatggc
     gaaacaaaaa ggtttttcaa aatctgcggt tgcggcgtta gctatagaag aatatgcaag
     aaaggaatca gaacaaaaaa aataagcgaa agctcgcgtt tttagaagga tacgagtttt
     cgctacttgt ttttgataag gtaattatat catggctatt aaaaatacta aagctagaaa
     ttttggattt ttattatatc ctgactcaat tcctaatgat tggaaagaaa aattagagag
     tttgggcgta tctatggctg tcagtccttt acacgatatg gacgaaaaaa aagataaaga
     tacatggaat aatagtaata ttatacaaaa tggaaagcac tataaaaaac cacactatca
     cgttatatat attgcacgaa atcctgtaac aatagaaagc gttaggaaca agattaagcg
     aaaattgggg aatagttcag ttgctcatgt tgagatactt gattatatca aaggttcata
     tgaatatttg actcatgaat caaaggacgc tattgctaag aataaacata tatacgacaa
     aaaagatatt ttgaacatta atgattttga tattgaccgc tatataacac ttgatgaaag
     ccaaaaaaga gaattgaaga atttactttt agatatagtg gatgactata atttggtaaa
     tacaaaagat ttaatggctt ttattcgcct taggggagcg gagtttggaa ttttaaatac
     gaatgatgta aaagatattg tttcaacaaa ctctagcgcc tttagattat ggtttgaggg
     caattatcag tgtggatata gagcaagtta tgcaaaggtt cttgatgctg aaacggggga
     aataaaatga caaacaaaga aaaagagtta tttgctgaaa atgaggaatt aaaaaaagaa
     attaaggact taaaagagcg tattgaaaga tacagagaaa tggaagttga attaagtaca
     acaatagatt tattgagagg agggattatt gaataaataa aagccccctg acgaaagtcg
     aagggggttt ttattttggt ttgatgttgc gattaatagc aatacaattg caataaacaa
     aatgatcttc cttcaggtta tgaccatctg tgccagttcg taatgtctgg tcaactttcc
     gactctgaga aacttctgga atcgctagag aatttctgga atgggattca ggagtggaca
     gaacgacacg gatatatagt ggatgtgtca aaacgcatac cattttgaac gatgacctct
     aataattgtt aatcatgttg gttacgtatt tattaacttc tcctagtatt agtaattatc
     atggctgtca tggcgcatta acggaataaa gggtgtgctt aaatcgggcc attttgcgta
     ataagaaaaa ggattaatta tgagcgaatt gaattaataa taaggtaata gatttacatt
     agaaaatgaa aggggatttt atgcgtgaga atgttacagt ctatccctgg cgaaaggggg
     atgtgctgca aggcgattaa gttgggtaac gccagggttt tcccagtcac gacgttgtaa
     aacgacggcc agtgagcgcg cgtaatacga ctcactatag ggcgaattgg gtaccgggcc
     ccccctcgag gtcgacggta tcgataagct tgatatcgaa ttcctgcagc ccgggggatc
     cactagttct agagcggccg ccaccgcggt ggagctccag cttttgttcc ctttagtgag
     ggttaattgc gcgcttggcg taatcatggt catagctgtt tcctgtgtga aattgttatc
     cgctcacaat tccacacaac atacgagccg gaagcataaa gtgtaaagcc tggggtgcct
     aatgagtgag ctaactcaca ttaattgcgt tgcgctcact gcccgctttc cagtcgggaa
     acctgtcgtg ccagnnnnnn nnnnnnnnn
//