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ID   PGHOST6    preliminary; circular DNA; SYN; 6722 BP.
XX
AC   IG0353;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Broad host range/E.coli plasmid vector pGhost6 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGhost6 from pGK series
RA   Maguin E.;
RT   ;
RL   Unpublished (1992).
XX
CC   NM (pGhost6)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Appligene)
CC   HO (E.coli)(Bacillus)(Clostridia)(Enterococcus)
CC   HO (Lactobacillus)(Lactococcus)(Leuconostoc)
CC   HO (Listeria)(Pediococcus)(Staphylococcus)
CC   HO (Streptococcus)(E.faecalis)(Gram-positive bacteria)
CC   CP ()
CC   FN (homologous chromosomal integration)(mutagenesis)(shuttle)
CC   FN (thermosensitive)(broad host range)(delivery vector)
CC   FN (cloning up to 7000 bp)
CC   SE ()
CC   PA (pGhost4)
CC   BR (pGhost5)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pGK series
FT                   -> pGhost6 6722bp"
FT   primer_bind     0..0
FT                   /note="PRI bacteriophage M13 -20"
FT   promoter        0..0
FT                   /note="PRO bacteriophage T7"
FT   misc_binding    0..0
FT                   /note="MCS unique KpnI-ApaI-XhoI-SalI-ClaI-HindIII-
FT                   EcoRV-EcoRI-SmaI-BamHI-SpeI-XbaI-XmaIII-NotI-SacII-
FT                   BstXI"
FT   primer_bind     0..0
FT                   /note="PRI KS"
FT   primer_bind     complement(0..0)
FT                   /note="PRI SK"
FT   promoter        complement(0..0)
FT                   /note="PRO bacteriophage T3"
FT   primer_bind     complement(0..0)
FT                   /note="PRI bacteriophage M13 reverse"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT non-unique PstI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="REP E. coli ROP gene"
FT   CDS             complement(0..0)
FT                   /note="ANT Streptococcus erythromycin resistance gene
FT                   (ery)"
FT   misc_binding    0..0
FT                   /note="SIT non-unique SacI"
FT   rep_origin      0..0
FT                   /note="ORI Gram positive bacteria pGK12 Ts"
XX
SQ   Sequence 6722 BP; 1961 A; 1278 C; 1503 G; 1980 T; 0 other;
     cgattcacaa aaaataggca cacgaaaaac aagttaaggg atgcagttta aattcttgaa
     gacgaaaggg cctcgtgata cgcctatttt tataggttaa tgtcatgata ataatggttt
     cttagacgtc aggtggcact tttcggggaa atgtgcgcgg aacccctatt tgtttatttt
     tctaaataca ttcaaatatg tatccgctca tgagacaata accctgataa atgcttcaat
     aatattgaaa aaggaagagt atgagtattc aacatttccg tgtcgccctt attccctttt
     ttgcggcatt ttgccttcct gtttttgctc acccagaaac gctggtgaaa gtaaaagatg
     ctgaagatca gttgggtgca cgagtgggtt acatcgaact ggatctcaac agcggtaaga
     tccttgagag ttttcgcccc gaagaacgtt ttccaatgat gagcactttt aaagttctgc
     tatgtggcgc ggtattatcc cgtgttgacg ccgggcaaga gcaactcggt cgccgcatac
     actattctca gaatgacttg gttgagtact caccagtcac agaaaagcat cttacggatg
     gcatgacagt aagagaatta tgcagtgctg ccataaccat gagtgataac actgcggcca
     acttacttct gacaacgatc ggaggaccga aggagctaac cgcttttttg cacaacatgg
     gggatcatgt aactcgcctt gatcgttggg aaccggagct gaatgaagcc ataccaaacg
     acgagcgtga caccacgatg cctgcagcaa tggcaacaac gttgcgcaaa ctattaactg
     gcgaactact tactctagct tcccggcaac aattaataga ctggatggag gcggataaag
     ttgcaggacc acttctgcgc tcggcccttc cggctggctg gtttattgct gataaatctg
     gagccggtga gcgtgggtct cgcggtatca ttgcagcact ggggccagat ggtaagccct
     cccgtatcgt agttatctac acgacgggga gtcaggcaac tatggatgaa cgaaatagac
     agatcgctga gataggtgcc tcactgatta agcattggta actgtcagac caagtttact
     catatatact ttagattgat ttaaaacttc atttttaatt taaaaggatc taggtgaaga
     tcctttttga taatctcatg accaaaatcc cttaacgtga gttttcgttc cactgagcgt
     cagaccccgt agaaaagatc aaaggatctt cttgagatcc tttttttctg cgcgtaatct
     gctgcttgca aacaaaaaaa ccaccgctac cagcggtggt ttgtttgccg gatcaagagc
     taccaactct ttttccgaag gtaactggct tcagcagagc gcagatacca aatactgtcc
     ttctagtgta gccgtagtta ggccaccact tcaagaactc tgtagcaccg cctacatacc
     tcgctctgct aatcctgtta ccagtggctg ctgccagtgg cgataagtcg tgtcttaccg
     ggttggactc aagacgatag ttaccggata aggcgcagcg gtcgggctga acggggggtt
     cgtgcacaca gcccagcttg gagcgaacga cctacaccga actgagatac ctacagcgtg
     agctatgaga aagcgccacg cttcccgaag ggagaaaggc ggacaggtat ccggtaagcg
     gcagggtcgg aacaggagag cgcacgaggg agcttccagg gggaaacgcc tggtatcttt
     atagtcctgt cgggtttcgc cacctctgac ttgagcgtcg atttttgtga tgctcgtcag
     gggggcggag cctatggaaa aacgccagca acgcggcctt tttacggttc ctggcctttt
     gctggccttt tgctcacatg ttctttcctg cgttatcccc tgattctgtg gataaccgta
     ttaccgcctt tgagtgagct gataccgctc gccgcagccg aacgaccgag cgcagcgagt
     cagtgagcga ggaagcggaa gagcgcctga tgcggtattt tctccttacg catctgtgcg
     gtatttcaca ccgcatatgg tgcactctca gtacaatctg ctctgatgcc gcatagttaa
     gccagtatac actccgctat cgctacgtga ctgggtcatg gctgcgcccc gacacccgcc
     aacacccgct gacgcgccct gacgggcttg tctgctcccg gcatccgctt acagacaagc
     tgtgaccgtc tccgggagct gcatgtgtca gaggttttca ccgtcatcac cgaaacgcgc
     gaggcagctg cggtaaagct catcagcgtg gtcgtgaagc gattcacaga tgtctgcctg
     ttcatccgcg tccagctcgt tgagtttctc cagaagcgtt aatgtctggc ttctgataaa
     gcgggccatg ttaagggcgg ttttttcctg tttggtcact gatgcctccg tgtaaggggg
     atttctgttc atgggggtaa tgataccgat gaaacgagag aggatgctca cgatacgggt
     tactgatgat gaacatgccc ggttactgga acgttgtgag ggtaaacaac tggcggtatg
     gatgcggcgg gaccagagaa aaatcactca gggtcaatgc cagcgcttcg ttaatacaga
     tgtaggtgtt ccacagggta gccagcagca tcctgcgatg cagatccgga acataatggt
     gcagggcgct gacttccgcg tttccagact ttacgaaaca cggaaaccga agaccattca
     tgttgttgct caggtcgcag acgttttgca gcagcagtcg cttcacgttc gctcgcgtat
     cggtgattca ttctgctaac cagtaaggca accccgccag cctagccggg tcctcaacga
     caggagcacg atcatgcgca cccgtggcca ggacccaacg ctgctccctt aacttactta
     ttaaataatt tatagctatt gaaaagagat aagaattgtt caaagctaat attgtttaaa
     tcgtcaattc ctgcatgttt taaggaattg ttaaattgat tttttgtaaa tattttcttg
     tattctttgt taacccattt cataacgaaa taattatact tttgtttatc tttgtgtgat
     attcttgatt tttttctact taatctgata agtgagctat tcactttagg tttaggatga
     aaatattctc ttggaaccat acttaatata gaaatatcaa cttctgccat taaaagtaat
     gccaatgagc gttttgtatt taataatctt ttagcaaacc cgtattccac gattaaataa
     atctcattag ctatactatc aaaaacaatt ttgcgtatta tatccgtact tatgttataa
     ggtatattac catatatttt ataggattgg tttttaggaa atttaaactg caatatatcc
     ttgtttaaaa cttggaaatt atcgtgatca acaagtttat tttctgtagt tttgcataat
     ttatggtcta tttcaatggc agttacgaaa ttacacctct ttactaattc aagggtaaaa
     tggccttttc ctgagccgat ttcaaagata ttatcatgtt catttaatct tatatttgtc
     attattttat ctatattatg ttttgaagta ataaagtttt gactgtgttt tatatttttc
     tcgttcatta taaccctctt taatttggtt atatgaattt tgcttattaa cgattcatta
     taaccactta ttttttgttt ggttgataat gaactgtgct gattacaaaa atactaaaaa
     tgcccatatt ttttcctcct tataaaatta gtataattat agcacgagct ctgataaata
     tgaacatgat gagtgatcgt taaatttata ctgcaatcgg atgcgattat tgaataaaag
     atatgagaga tttatctaat ttcttttttc ttgtaaaaaa agaaagttct taaaggtttt
     atagttttgg tcgtagagca cacggtttaa cgacttaatt acgaagtaaa taagtctagt
     gtgttagact ttatgaaatc tatatacgtt tatatatatt tattatccga ttttttatta
     aaacgtctca aaatcgtttc tgagacgttt tagcgtttat ttcgtttagt tatcggcata
     atcgttaaaa caggcgttat cgtagcgtaa aagcccttga gcgtagcgtg gctttgcagc
     gaagatgttg tctgttagat tatgaaagcc gatgactgaa tgaaataata agcgcagcgc
     ccttctattt cggttggagg aggctcaagg gagtatgagg gaatgaaatt ccctcatggg
     tttgatttta aaaattgctt gcaattttgc cgagcggtag cgctggaaaa tttttgaaaa
     aaatttggaa tttggaaaaa aatgggggga aaggaagcga attttgcttc cgtactacga
     ccccccatta agtgccgagt gccaattttt gtgccaaaaa cgctctatcc caactggctc
     aagggtttaa ggggtttttc aatcgccaac gaatcgccaa cgttttcgcc aacgtttttt
     ataaatctat atttaagtag ctttattgtt gtttttatga ttacaaagtg atacactaac
     tttataaaat tatttgattg gagtttttta aatggtgatt tcagaatcga aaaaaagagt
     tatgatttct ctgacaaaag agcaagataa aaaattaaca gatatggcga aacaaaaagg
     tttttcaaaa tctgcggttg cggcgttagc tatagaagaa tatgcaagaa aggaatcaga
     acaaaaaaaa taagcgaaag ctcgcgtttt tagaaggata cgagttttcg ctacttgttt
     ttgataaggt aattatatca tggctattaa aaatactaaa gctagaaatt ttggattttt
     attatatcct gactcaattc ctaatgattg gaaagaaaaa ttagagagtt tgggcgtatc
     tatggctgtc agtcctttac acgatatgga cgaaaaaaaa gataaagata catggaataa
     tagtaatatt atacaaaatg gaaagcacta taaaaaacca cactatcacg ttatatatat
     tgcacgaaat cctgtaacaa tagaaagcgt taggaacaag attaagcgaa aattggggaa
     tagttcagtt gctcatgttg agatacttga ttatatcaaa ggttcatatg aatatttgac
     tcatgaatca aaggacgcta ttgctaagaa taaacatata tacgacaaaa aagatatttt
     gaacattaat gattttgata ttgaccgcta tataacactt gatgaaagcc aaaaaagaga
     attgaagaat ttacttttag atatagtgga tgactataat ttggtaaata caaaagattt
     aatggctttt attcgcctta ggggagcgga gtttggaatt ttaaatacga atgatgtaaa
     agatattgtt tcaacaaact ctagcgcctt tagattatgg tttgagggca attatcagtg
     tggatataga gcaagttatg caaaggttct tgatgctgaa acgggggaaa taaaatgaca
     aacaaagaaa aagagttatt tgctgaaaat gaggaattaa aaaaagaaat taaggactta
     aaagagcgta ttgaaagata cagagaaatg gaagttgaat taagtacaac aatagattta
     ttgagaggag ggattattga ataaataaaa gccccctgac gaaagtcgaa gggggttttt
     attttggttt gatgttgcga ttaatagcaa tacaattgca ataaacaaaa tgatcttcct
     tcaggttatg accatctgtg ccagttcgta atgtctggtc aactttccga ctctgagaaa
     cttctggaat cgctagagaa tttctggaat gggattcagg agtggacaga acgacacgga
     tatatagtgg atgtgtcaaa acgcatacca ttttgaacga tgacctctaa taattgttaa
     tcatgttggt tacgtattta ttaacttctc ctagtattag taattatcat ggctgtcatg
     gcgcattaac ggaataaagg gtgtgcttaa atcgggccat tttgcgtaat aagaaaaagg
     attaattatg agcgaattga attaataata aggtaataga tttacattag aaaatgaaag
     gggattttat gcgtgagaat gttacagtct atccctggcg aaagggggat gtgctgcaag
     gcgattaagt tgggtaacgc cagggttttc ccagtcacga cgttgtaaaa cgacggccag
     tgagcgcgcg taatacgact cactataggg cgaattgggt accgggcccc ccctcgaggt
     cgacggtatc gataagcttg atatcgaatt cctgcagccc gggggatcca ctagttctag
     agcggccgcc accgcggtgg agctccagct tttgttccct ttagtgaggg ttaattgcgc
     gcttggcgta atcatggtca tagctgtttc ctgtgtgaaa ttgttatccg ctcacaattc
     cacacaacat acgagccgga agcataaagt gtaaagcctg gggtgcctaa tgagtgagct
     aactcacatt aattgcgttg cgctcactgc ccgctttcca gtcgggaaac ctgtcgtgcc
     ag
//