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ID   PGL2BASIC  preliminary; circular DNA; SYN; 5597 BP.
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AC   X65323; IG0157;
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DT   05-SEP-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGL2-Basic - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGL2-Basic, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-5597
RC   pGL2-Basic
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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CC   pGL2-Basic has no enhancer or promoter.
CC   NM (pGL2-Basic)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli JM109)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (f1)(pBR322)
CC   BR (pGL2-Enhancer)(pGL2-Promoter)(pGL2-Control)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. firefly HindIII-? 1700bp, luciferase gene
FT                   3. SV40, UTR
FT                   4. f1, ori
FT                   -> pGL2-Basic 5597bp"
FT   misc_binding    3..47
FT                   /note="MCS
FT                   SmaI-KpnI-SacI-MluI-NheI-XhoI-BglII-HindIII"
FT   CDS             76..1728
FT                   /note="GEN firefly luciferase gene (luxA)"
FT   polyA_signal    0..0
FT                   /note="PLA E. coli luc gene"
FT   misc_feature    1892..2743
FT                   /note="SV40 3' untranslated region"
FT   misc_binding    2043..2043
FT                   /note="SIT unique PfiMI"
FT   misc_binding    2738..2738
FT                   /note="SIT unique BamHI"
FT   misc_binding    2744..2744
FT                   /note="SIT unique SalI"
FT   rep_origin      complement(3052..3052)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(3814..4674)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      4806..5261
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   polyA_signal    0..0
FT                   /note="PLA for background reduction"
XX
SQ   Sequence 5597 BP; 1523 A; 1261 C; 1269 G; 1544 T; 0 other;
     cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttggcattcc
     ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
     tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
     attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
     acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
     atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
     tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
     tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
     gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
     ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     aaagagatcg tggattacgt ggccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
     ttcagcgatg acgaaattct tagctattgt aatactgcga tgagtggcag ggcggggcgt
     aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct gaataagtga
     taataagcgg atgaatggca gaaattcgcc ggatctttgt gaaggaacct tacttctgtg
     gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
     gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
     tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
     gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     ggtttgtcca aactcatcaa tgtatcttat catgtctgga tccgtcgacc gatgcccttg
     agagccttca acccagtcag ctccttccgg tgggcgcggg gcatgactat cgtcgccgca
     cttatgactg tcttctttat catgcaactc gtaggacagg tgccggcagc gctcttccgc
     ttcctcgctc actgactcgc tgcgctcggt cgttcggctg cggcgagcgg tatcagctca
     ctcaaaggcg gtaatacggt tatccacaga atcaggggat aacgcaggaa agaacatgtg
     agcaaaaggc cagcaaaagg ccaggaaccg taaaaaggcc gcgttgctgg cgtttttcca
     taggctccgc ccccctgacg agcatcacaa aaatcgacgc tcaagtcaga ggtggcgaaa
     cccgacagga ctataaagat accaggcgtt tccccctgga agctccctcg tgcgctctcc
     tgttccgacc ctgccgctta ccggatacct gtccgccttt ctcccttcgg gaagcgtggc
     gctttctcaa tgctcacgct gtaggtatct cagttcggtg taggtcgttc gctccaagct
     gggctgtgtg cacgaacccc ccgttcagcc cgaccgctgc gccttatccg gtaactatcg
     tcttgagtcc aacccggtaa gacacgactt atcgccactg gcagcagcca ctggtaacag
     gattagcaga gcgaggtatg taggcggtgc tacagagttc ttgaagtggt ggcctaacta
     cggctacact agaaggacag tatttggtat ctgcgctctg ctgaagccag ttaccttcgg
     aaaaagagtt ggtagctctt gatccggcaa acaaaccacc gctggtagcg gtggtttttt
     tgtttgcaag cagcagatta cgcgcagaaa aaaaggatct caagaagatc ctttgatctt
     ttctacgggg tctgacgctc agtggaacga aaactcacgt taagggattt tggtcatgag
     attatcaaaa aggatcttca cctagatcct tttaaattaa aaatgaagtt ttaaatcaat
     ctaaagtata tatgagtaaa cttggtctga cagttaccaa tgcttaatca gtgaggcacc
     tatctcagcg atctgtctat ttcgttcatc catagttgcc tgactccccg tcgtgtagat
     aactacgata cgggagggct taccatctgg ccccagtgct gcaatgatac cgcgagaccc
     acgctcaccg gctccagatt tatcagcaat aaaccagcca gccggaaggg ccgagcgcag
     aagtggtcct gcaactttat ccgcctccat ccagtctatt aattgttgcc gggaagctag
     agtaagtagt tcgccagtta atagtttgcg caacgttgtt gccattgcta caggcatcgt
     ggtgtcacgc tcgtcgtttg gtatggcttc attcagctcc ggttcccaac gatcaaggcg
     agttacatga tcccccatgt tgtgcaaaaa agcggttagc tccttcggtc ctccgatcgt
     tgtcagaagt aagttggccg cagtgttatc actcatggtt atggcagcac tgcataattc
     tcttactgtc atgccatccg taagatgctt ttctgtgact ggtgagtact caaccaagtc
     attctgagaa tagtgtatgc ggcgaccgag ttgctcttgc ccggcgtcaa tacgggataa
     taccgcgcca catagcagaa ctttaaaagt gctcatcatt ggaaaacgtt cttcggggcg
     aaaactctca aggatcttac cgctgttgag atccagttcg atgtaaccca ctcgtgcacc
     caactgatct tcagcatctt ttactttcac cagcgtttct gggtgagcaa aaacaggaag
     gcaaaatgcc gcaaaaaagg gaataagggc gacacggaaa tgttgaatac tcatactctt
     cctttttcaa tattattgaa gcatttatca gggttattgt ctcatgagcg gatacatatt
     tgaatgtatt tagaaaaata aacaaatagg ggttccgcgc acatttcccc gaaaagtgcc
     acctgacgcg ccctgtagcg gcgcattaag cgcggcgggt gtggtggtta cgcgcagcgt
     gaccgctaca cttgccagcg ccctagcgcc cgctcctttc gctttcttcc cttcctttct
     cgccacgttc gccggctttc cccgtcaagc tctaaatcgg gggctccctt tagggttccg
     atttagtgct ttacggcacc tcgaccccaa aaaacttgat tagggtgatg gttcacgtag
     tgggccatcg ccctgataga cggtttttcg ccctttgacg ttggagtcca cgttctttaa
     tagtggactc ttgttccaaa ctggaacaac actcaaccct atctcggtct attcttttga
     tttataaggg attttgccga tttcggccta ttggttaaaa aatgagctga tttaacaaaa
     atttaacgcg aattttaaca aaatattaac gtttacaatt tcccattcgc cattcaggct
     gcgcaactgt tgggaagggc gatcggtgcg ggcctcttcg ctattacgcc agcccaagct
     accatgataa gtaagtaata ttaaggtacg tggaggtttt acttgcttta aaaaacctcc
     cacacctccc cctgaacctg aaacataaaa tgaatgcaat tgttgttgtt aacttgttta
     ttgcagctta taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat
     ttttttcact gcattctagt tgtggtttgt ccaaactcat caatgtatct tatggtactg
     taactgagct aacataa
//