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ID   PGL2CONTR  preliminary; circular DNA; SYN; 6046 BP.
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AC   X65324; IG0158;
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DT   05-SEP-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGL2-Control - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGL2-Control, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-6046
RC   pGL2-Control
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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CC   pGL2-Control has both enhancer and promoter. has 6046 bp.
CC   NM (pGL2-Control)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli JM109)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (f1)(pBR322)
CC   BR (pGL2-Enhancer)(pGL2-Basic)(pGL2-Basic)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. firefly HindIII-? 1700bp, luciferase gene
FT                   3. SV40, UTR
FT                   4. f1, ori
FT                   -> pGL2-Control 6046bp"
FT   misc_binding    3..37
FT                   /note="MCS
FT                   SmaI-KpnI-SacI-MluI-NheI-XhoI-BglII-HindIII"
FT   promoter        43..238
FT                   /note="PRO SV40 promoter"
FT   misc_binding    239..239
FT                   /note="SIT unique HindIII"
FT   CDS             268..1920
FT                   /note="GEN firefly luciferase gene (luxA)"
FT   misc_feature    2084..2934
FT                   /note="SV40 3' untranslated region"
FT   misc_binding    2235..2235
FT                   /note="SIT unique PfiMI"
FT   polyA_signal    0..0
FT                   /note="PLA E. coli luc gene"
FT   promoter        2931..3176
FT                   /note="PRO SV40 enhancer"
FT   misc_binding    3187..3187
FT                   /note="SIT unique BamHI"
FT   misc_binding    3193..3193
FT                   /note="SIT unique SalI"
FT   rep_origin      complement(3501..3501)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(4263..5123)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      5255..5710
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   polyA_signal    0..0
FT                   /note="PLA for background reduction"
XX
SQ   Sequence 6046 BP; 1611 A; 1386 C; 1393 G; 1656 T; 0 other;
     cccgggaggt accgagctct tacgcgtgct agctcgagat ctgcatctca attagtcagc
     aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     gcttggcatt ccggtactgt tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg
     gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag
     agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc
     acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag
     gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc
     atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca
     attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg
     catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc
     attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta
     ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc
     catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt
     acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt
     gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca ggtggccccc
     gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt
     cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     acgatgacgg aaaaagagat cgtggattac gtggccagtc aagtaacaac cgcgaaaaag
     ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa
     aatgtaactg tattcagcga tgacgaaatt cttagctatt gtaatactgc gatgagtggc
     agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggtgctacgc
     ctgaataagt gataataagc ggatgaatgg cagaaattcg ccggatcttt gtgaaggaac
     cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatctgaacc
     atggagcgga gaatgggcgg aactgggcgg agttaggggc gggatgggcg gagttagggg
     cgggactatg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     gcctggggac tttccacacc tggttgctga ctaattgaga tgcatgcttt gcatacttct
     gcctgctggg gagcctgggg actttccaca ccctaactga cacacattcc acagcagatc
     ctctagggat ccgtcgaccg atgcccttga gagccttcaa cccagtcagc tccttccggt
     gggcgcgggg catgactatc gtcgccgcac ttatgactgt cttctttatc atgcaactcg
     taggacaggt gccggcagcg ctcttccgct tcctcgctca ctgactcgct gcgctcggtc
     gttcggctgc ggcgagcggt atcagctcac tcaaaggcgg taatacggtt atccacagaa
     tcaggggata acgcaggaaa gaacatgtga gcaaaaggcc agcaaaaggc caggaaccgt
     aaaaaggccg cgttgctggc gtttttccat aggctccgcc cccctgacga gcatcacaaa
     aatcgacgct caagtcagag gtggcgaaac ccgacaggac tataaagata ccaggcgttt
     ccccctggaa gctccctcgt gcgctctcct gttccgaccc tgccgcttac cggatacctg
     tccgcctttc tcccttcggg aagcgtggcg ctttctcaat gctcacgctg taggtatctc
     agttcggtgt aggtcgttcg ctccaagctg ggctgtgtgc acgaaccccc cgttcagccc
     gaccgctgcg ccttatccgg taactatcgt cttgagtcca acccggtaag acacgactta
     tcgccactgg cagcagccac tggtaacagg attagcagag cgaggtatgt aggcggtgct
     acagagttct tgaagtggtg gcctaactac ggctacacta gaaggacagt atttggtatc
     tgcgctctgc tgaagccagt taccttcgga aaaagagttg gtagctcttg atccggcaaa
     caaaccaccg ctggtagcgg tggttttttt gtttgcaagc agcagattac gcgcagaaaa
     aaaggatctc aagaagatcc tttgatcttt tctacggggt ctgacgctca gtggaacgaa
     aactcacgtt aagggatttt ggtcatgaga ttatcaaaaa ggatcttcac ctagatcctt
     ttaaattaaa aatgaagttt taaatcaatc taaagtatat atgagtaaac ttggtctgac
     agttaccaat gcttaatcag tgaggcacct atctcagcga tctgtctatt tcgttcatcc
     atagttgcct gactccccgt cgtgtagata actacgatac gggagggctt accatctggc
     cccagtgctg caatgatacc gcgagaccca cgctcaccgg ctccagattt atcagcaata
     aaccagccag ccggaagggc cgagcgcaga agtggtcctg caactttatc cgcctccatc
     cagtctatta attgttgccg ggaagctaga gtaagtagtt cgccagttaa tagtttgcgc
     aacgttgttg ccattgctac aggcatcgtg gtgtcacgct cgtcgtttgg tatggcttca
     ttcagctccg gttcccaacg atcaaggcga gttacatgat cccccatgtt gtgcaaaaaa
     gcggttagct ccttcggtcc tccgatcgtt gtcagaagta agttggccgc agtgttatca
     ctcatggtta tggcagcact gcataattct cttactgtca tgccatccgt aagatgcttt
     tctgtgactg gtgagtactc aaccaagtca ttctgagaat agtgtatgcg gcgaccgagt
     tgctcttgcc cggcgtcaat acgggataat accgcgccac atagcagaac tttaaaagtg
     ctcatcattg gaaaacgttc ttcggggcga aaactctcaa ggatcttacc gctgttgaga
     tccagttcga tgtaacccac tcgtgcaccc aactgatctt cagcatcttt tactttcacc
     agcgtttctg ggtgagcaaa aacaggaagg caaaatgccg caaaaaaggg aataagggcg
     acacggaaat gttgaatact catactcttc ctttttcaat attattgaag catttatcag
     ggttattgtc tcatgagcgg atacatattt gaatgtattt agaaaaataa acaaataggg
     gttccgcgca catttccccg aaaagtgcca cctgacgcgc cctgtagcgg cgcattaagc
     gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc
     gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct
     ctaaatcggg ggctcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa
     aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc
     cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca
     ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttgccgat ttcggcctat
     tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attttaacaa aatattaacg
     tttacaattt cccattcgcc attcaggctg cgcaactgtt gggaagggcg atcggtgcgg
     gcctcttcgc tattacgcca gcccaagcta ccatgataag taagtaatat taaggtacgt
     ggaggtttta cttgctttaa aaaacctccc acacctcccc ctgaacctga aacataaaat
     gaatgcaatt gttgttgtta acttgtttat tgcagcttat aatggttaca aataaagcaa
     tagcatcaca aatttcacaa ataaagcatt tttttcactg cattctagtt gtggtttgtc
     caaactcatc aatgtatctt atggtactgt aactgagcta acataa
//