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ID   PGL2ENHAN  preliminary; circular DNA; SYN; 5854 BP.
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AC   X65325; IG0159;
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DT   05-SEP-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli phagemid vector pGL2-Enhancer - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGL2-Enhancer, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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RN   [2]
RP   1-5854
RC   pGL2-Enhancer
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
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CC   pGL2-Enhancer has no promoter.
CC   NM (pGL2-Enhancer)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (Promega)
CC   HO (E.coli JM109)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (f1)(pBR322)
CC   BR (pGL2-Basic)(pGL2-Promoter)(pGL2-Control)
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. firefly HindIII-? 1700bp, luciferase gene
FT                   3. SV40, UTR
FT                   4. f1, ori
FT                   -> pGL2-Enhancer 5854bp"
FT   misc_binding    3..47
FT                   /note="MCS
FT                   SmaI-KpnI-SacI-MluI-NheI-XhoI-BglII-HindIII"
FT   CDS             76..1728
FT                   /note="GEN firefly luciferase gene (luxA)"
FT   misc_feature    1892..2742
FT                   /note="SV40 3' untranslated region"
FT   misc_binding    2043..2043
FT                   /note="SIT unique PfiMI"
FT   polyA_signal    0..0
FT                   /note="PLA E. coli luc gene"
FT   promoter        2739..2984
FT                   /note="PRO SV40 enhancer"
FT   misc_binding    2995..2995
FT                   /note="SIT unique BamHI"
FT   misc_binding    3001..3001
FT                   /note="SIT unique SalI"
FT   rep_origin      complement(3309..3309)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(4071..4931)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      5063..5518
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   polyA_signal    0..0
FT                   /note="PLA for background reduction"
XX
SQ   Sequence 5854 BP; 1575 A; 1320 C; 1353 G; 1606 T; 0 other;
     cccgggaggt accgagctct tacgcgtgct agctcgagat ctaagtaagc ttggcattcc
     ggtactgttg gtaaaatgga agacgccaaa aacataaaga aaggcccggc gccattctat
     cctctagagg atggaaccgc tggagagcaa ctgcataagg ctatgaagag atacgccctg
     gttcctggaa caattgcttt tacagatgca catatcgagg tgaacatcac gtacgcggaa
     tacttcgaaa tgtccgttcg gttggcagaa gctatgaaac gatatgggct gaatacaaat
     cacagaatcg tcgtatgcag tgaaaactct cttcaattct ttatgccggt gttgggcgcg
     ttatttatcg gagttgcagt tgcgcccgcg aacgacattt ataatgaacg tgaattgctc
     aacagtatga acatttcgca gcctaccgta gtgtttgttt ccaaaaaggg gttgcaaaaa
     attttgaacg tgcaaaaaaa attaccaata atccagaaaa ttattatcat ggattctaaa
     acggattacc agggatttca gtcgatgtac acgttcgtca catctcatct acctcccggt
     tttaatgaat acgattttgt accagagtcc tttgatcgtg acaaaacaat tgcactgata
     atgaattcct ctggatctac tgggttacct aagggtgtgg cccttccgca tagaactgcc
     tgcgtcagat tctcgcatgc cagagatcct atttttggca atcaaatcat tccggatact
     gcgattttaa gtgttgttcc attccatcac ggttttggaa tgtttactac actcggatat
     ttgatatgtg gatttcgagt cgtcttaatg tatagatttg aagaagagct gtttttacga
     tcccttcagg attacaaaat tcaaagtgcg ttgctagtac caaccctatt ttcattcttc
     gccaaaagca ctctgattga caaatacgat ttatctaatt tacacgaaat tgcttctggg
     ggcgcacctc tttcgaaaga agtcggggaa gcggttgcaa aacgcttcca tcttccaggg
     atacgacaag gatatgggct cactgagact acatcagcta ttctgattac acccgagggg
     gatgataaac cgggcgcggt cggtaaagtt gttccatttt ttgaagcgaa ggttgtggat
     ctggataccg ggaaaacgct gggcgttaat cagagaggcg aattatgtgt cagaggacct
     atgattatgt ccggttatgt aaacaatccg gaagcgacca acgccttgat tgacaaggat
     ggatggctac attctggaga catagcttac tgggacgaag acgaacactt cttcatagtt
     gaccgcttga agtctttaat taaatacaaa ggatatcagg tggcccccgc tgaattggaa
     tcgatattgt tacaacaccc caacatcttc gacgcgggcg tggcaggtct tcccgacgat
     gacgccggtg aacttcccgc cgccgttgtt gttttggagc acggaaagac gatgacggaa
     aaagagatcg tggattacgt ggccagtcaa gtaacaaccg cgaaaaagtt gcgcggagga
     gttgtgtttg tggacgaagt accgaaaggt cttaccggaa aactcgacgc aagaaaaatc
     agagagatcc tcataaaggc caagaagggc ggaaagtcca aattgtaaaa tgtaactgta
     ttcagcgatg acgaaattct tagctattgt aatactgcga tgagtggcag ggcggggcgt
     aattttttta aggcagttat tggtgccctt aaacgcctgg tgctacgcct gaataagtga
     taataagcgg atgaatggca gaaattcgcc ggatctttgt gaaggaacct tacttctgtg
     gtgtgacata attggacaaa ctacctacag agatttaaag ctctaaggta aatataaaat
     ttttaagtgt ataatgtgtt aaactactga ttctaattgt ttgtgtattt tagattccaa
     cctatggaac tgatgaatgg gagcagtggt ggaatgcctt taatgaggaa aacctgtttt
     gctcagaaga aatgccatct agtgatgatg aggctactgc tgactctcaa cattctactc
     ctccaaaaaa gaagagaaag gtagaagacc ccaaggactt tccttcagaa ttgctaagtt
     ttttgagtca tgctgtgttt agtaatagaa ctcttgcttg ctttgctatt tacaccacaa
     aggaaaaagc tgcactgcta tacaagaaaa ttatggaaaa atattctgta acctttataa
     gtaggcataa cagttataat cataacatac tgttttttct tactccacac aggcatagag
     tgtctgctat taataactat gctcaaaaat tgtgtacctt tagcttttta atttgtaaag
     gggttaataa ggaatatttg atgtatagtg ccttgactag agatcataat cagccatacc
     acatttgtag aggttttact tgctttaaaa aacctcccac acctccccct gaacctgaaa
     cataaaatga atgcaattgt tgttgttaac ttgtttattg cagcttataa tggttacaaa
     taaagcaata gcatcacaaa tttcacaaat aaagcatttt tttcactgca ttctagttgt
     ggtttgtcca aactcatcaa tgtatcttat catgtctgga tctgaaccat ggagcggaga
     atgggcggaa ctgggcggag ttaggggcgg gatgggcgga gttaggggcg ggactatggt
     tgctgactaa ttgagatgca tgctttgcat acttctgcct gctggggagc ctggggactt
     tccacacctg gttgctgact aattgagatg catgctttgc atacttctgc ctgctgggga
     gcctggggac tttccacacc ctaactgaca cacattccac agcagatcct ctagggatcc
     gtcgaccgat gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca
     tgactatcgt cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc
     cggcagcgct cttccgcttc ctcgctcact gactcgctgc gctcggtcgt tcggctgcgg
     cgagcggtat cagctcactc aaaggcggta atacggttat ccacagaatc aggggataac
     gcaggaaaga acatgtgagc aaaaggccag caaaaggcca ggaaccgtaa aaaggccgcg
     ttgctggcgt ttttccatag gctccgcccc cctgacgagc atcacaaaaa tcgacgctca
     agtcagaggt ggcgaaaccc gacaggacta taaagatacc aggcgtttcc ccctggaagc
     tccctcgtgc gctctcctgt tccgaccctg ccgcttaccg gatacctgtc cgcctttctc
     ccttcgggaa gcgtggcgct ttctcaatgc tcacgctgta ggtatctcag ttcggtgtag
     gtcgttcgct ccaagctggg ctgtgtgcac gaaccccccg ttcagcccga ccgctgcgcc
     ttatccggta actatcgtct tgagtccaac ccggtaagac acgacttatc gccactggca
     gcagccactg gtaacaggat tagcagagcg aggtatgtag gcggtgctac agagttcttg
     aagtggtggc ctaactacgg ctacactaga aggacagtat ttggtatctg cgctctgctg
     aagccagtta ccttcggaaa aagagttggt agctcttgat ccggcaaaca aaccaccgct
     ggtagcggtg gtttttttgt ttgcaagcag cagattacgc gcagaaaaaa aggatctcaa
     gaagatcctt tgatcttttc tacggggtct gacgctcagt ggaacgaaaa ctcacgttaa
     gggattttgg tcatgagatt atcaaaaagg atcttcacct agatcctttt aaattaaaaa
     tgaagtttta aatcaatcta aagtatatat gagtaaactt ggtctgacag ttaccaatgc
     ttaatcagtg aggcacctat ctcagcgatc tgtctatttc gttcatccat agttgcctga
     ctccccgtcg tgtagataac tacgatacgg gagggcttac catctggccc cagtgctgca
     atgataccgc gagacccacg ctcaccggct ccagatttat cagcaataaa ccagccagcc
     ggaagggccg agcgcagaag tggtcctgca actttatccg cctccatcca gtctattaat
     tgttgccggg aagctagagt aagtagttcg ccagttaata gtttgcgcaa cgttgttgcc
     attgctacag gcatcgtggt gtcacgctcg tcgtttggta tggcttcatt cagctccggt
     tcccaacgat caaggcgagt tacatgatcc cccatgttgt gcaaaaaagc ggttagctcc
     ttcggtcctc cgatcgttgt cagaagtaag ttggccgcag tgttatcact catggttatg
     gcagcactgc ataattctct tactgtcatg ccatccgtaa gatgcttttc tgtgactggt
     gagtactcaa ccaagtcatt ctgagaatag tgtatgcggc gaccgagttg ctcttgcccg
     gcgtcaatac gggataatac cgcgccacat agcagaactt taaaagtgct catcattgga
     aaacgttctt cggggcgaaa actctcaagg atcttaccgc tgttgagatc cagttcgatg
     taacccactc gtgcacccaa ctgatcttca gcatctttta ctttcaccag cgtttctggg
     tgagcaaaaa caggaaggca aaatgccgca aaaaagggaa taagggcgac acggaaatgt
     tgaatactca tactcttcct ttttcaatat tattgaagca tttatcaggg ttattgtctc
     atgagcggat acatatttga atgtatttag aaaaataaac aaataggggt tccgcgcaca
     tttccccgaa aagtgccacc tgacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg
     gtggttacgc gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct
     ttcttccctt cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg
     ctccctttag ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgattag
     ggtgatggtt cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg
     gagtccacgt tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc
     tcggtctatt cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat
     gagctgattt aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttcc
     cattcgccat tcaggctgcg caactgttgg gaagggcgat cggtgcgggc ctcttcgcta
     ttacgccagc ccaagctacc atgataagta agtaatatta aggtacgtgg aggttttact
     tgctttaaaa aacctcccac acctccccct gaacctgaaa cataaaatga atgcaattgt
     tgttgttaac ttgtttattg cagcttataa tggttacaaa taaagcaata gcatcacaaa
     tttcacaaat aaagcatttt tttcactgca ttctagttgt ggtttgtcca aactcatcaa
     tgtatcttat ggtactgtaa ctgagctaac ataa
//