back Return to this vector's summary.
ID   PGL2PROMO  preliminary; circular DNA; SYN; 5789 BP.
XX
AC   X65326; IG0160;
XX
DT   05-SEP-1991 (Rel. 6, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pGL2-Promoter - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGL2-Promoter, revised by [2]
RA   ;
RT   ;
RL   Submitted (23-MAR-1992) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
RN   [2]
RP   1-5789
RC   pGL2-Promoter
RA   Klock C.;
RT   ;
RL   Submitted (28-MAY-1993) on tape to the EMBL Data Library by:
RL   Technical Services, Promega Corporation, 2800 Woods Hollow Road,
RL   Madison, WI 53711-5399, USA.
XX
CC   pGL2-Promoter has promoter but no enhancer.
CC   NM (pGL2-Promoter)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (Promega)
CC   HO (E.coli JM109)(mammal)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (f1)(pBR322)
CC   BR (pGL2-Enhancer)(pGL2-Basic)(pGL2-Control)
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 4361bp, ori/amp gene
FT                   2. firefly HindIII-? 1700bp, luciferase gene
FT                   3. SV40, UTR
FT                   4. f1, ori
FT                   -> pGL2-Promoter 5789bp"
FT   misc_binding    3..37
FT                   /note="MCS
FT                   SmaI-KpnI-SacI-MluI-NheI-XhoI-BglII"
FT   promoter        43..238
FT                   /note="PRO SV40 promoter"
FT   misc_binding    239..239
FT                   /note="SIT unique HindIII"
FT   CDS             268..1920
FT                   /note="GEN firefly luciferase gene (luxA)"
FT   misc_feature    2084..2935
FT                   /note="SV40 3' untranslated region"
FT   misc_binding    2235..2235
FT                   /note="SIT unique PfiMI"
FT   polyA_signal    0..0
FT                   /note="PLA E. coli luc gene"
FT   misc_binding    2930..2930
FT                   /note="SIT unique BamHI"
FT   misc_binding    2936..2936
FT                   /note="SIT unique SalI"
FT   rep_origin      complement(3244..3244)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(4006..4866)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   rep_origin      4998..5453
FT                   /note="ORI bacteriophage f1 intergenic region"
FT   polyA_signal    0..0
FT                   /note="PLA for background reduction"
XX
SQ   Sequence 5789 BP; 1559 A; 1327 C; 1309 G; 1594 T; 0 other;
     cccgggaggt accgagctct tacgcgtgct agctcgagat ctgcatctca attagtcagc
     aaccatagtc ccgcccctaa ctccgcccat cccgccccta actccgccca gttccgccca
     ttctccgccc catggctgac taattttttt tatttatgca gaggccgagg ccgcctcggc
     ctctgagcta ttccagaagt agtgaggagg cttttttgga ggcctaggct tttgcaaaaa
     gcttggcatt ccggtactgt tggtaaaatg gaagacgcca aaaacataaa gaaaggcccg
     gcgccattct atcctctaga ggatggaacc gctggagagc aactgcataa ggctatgaag
     agatacgccc tggttcctgg aacaattgct tttacagatg cacatatcga ggtgaacatc
     acgtacgcgg aatacttcga aatgtccgtt cggttggcag aagctatgaa acgatatggg
     ctgaatacaa atcacagaat cgtcgtatgc agtgaaaact ctcttcaatt ctttatgccg
     gtgttgggcg cgttatttat cggagttgca gttgcgcccg cgaacgacat ttataatgaa
     cgtgaattgc tcaacagtat gaacatttcg cagcctaccg tagtgtttgt ttccaaaaag
     gggttgcaaa aaattttgaa cgtgcaaaaa aaattaccaa taatccagaa aattattatc
     atggattcta aaacggatta ccagggattt cagtcgatgt acacgttcgt cacatctcat
     ctacctcccg gttttaatga atacgatttt gtaccagagt cctttgatcg tgacaaaaca
     attgcactga taatgaattc ctctggatct actgggttac ctaagggtgt ggcccttccg
     catagaactg cctgcgtcag attctcgcat gccagagatc ctatttttgg caatcaaatc
     attccggata ctgcgatttt aagtgttgtt ccattccatc acggttttgg aatgtttact
     acactcggat atttgatatg tggatttcga gtcgtcttaa tgtatagatt tgaagaagag
     ctgtttttac gatcccttca ggattacaaa attcaaagtg cgttgctagt accaacccta
     ttttcattct tcgccaaaag cactctgatt gacaaatacg atttatctaa tttacacgaa
     attgcttctg ggggcgcacc tctttcgaaa gaagtcgggg aagcggttgc aaaacgcttc
     catcttccag ggatacgaca aggatatggg ctcactgaga ctacatcagc tattctgatt
     acacccgagg gggatgataa accgggcgcg gtcggtaaag ttgttccatt ttttgaagcg
     aaggttgtgg atctggatac cgggaaaacg ctgggcgtta atcagagagg cgaattatgt
     gtcagaggac ctatgattat gtccggttat gtaaacaatc cggaagcgac caacgccttg
     attgacaagg atggatggct acattctgga gacatagctt actgggacga agacgaacac
     ttcttcatag ttgaccgctt gaagtcttta attaaataca aaggatatca ggtggccccc
     gctgaattgg aatcgatatt gttacaacac cccaacatct tcgacgcggg cgtggcaggt
     cttcccgacg atgacgccgg tgaacttccc gccgccgttg ttgttttgga gcacggaaag
     acgatgacgg aaaaagagat cgtggattac gtggccagtc aagtaacaac cgcgaaaaag
     ttgcgcggag gagttgtgtt tgtggacgaa gtaccgaaag gtcttaccgg aaaactcgac
     gcaagaaaaa tcagagagat cctcataaag gccaagaagg gcggaaagtc caaattgtaa
     aatgtaactg tattcagcga tgacgaaatt cttagctatt gtaatactgc gatgagtggc
     agggcggggc gtaatttttt taaggcagtt attggtgccc ttaaacgcct ggtgctacgc
     ctgaataagt gataataagc ggatgaatgg cagaaattcg ccggatcttt gtgaaggaac
     cttacttctg tggtgtgaca taattggaca aactacctac agagatttaa agctctaagg
     taaatataaa atttttaagt gtataatgtg ttaaactact gattctaatt gtttgtgtat
     tttagattcc aacctatgga actgatgaat gggagcagtg gtggaatgcc tttaatgagg
     aaaacctgtt ttgctcagaa gaaatgccat ctagtgatga tgaggctact gctgactctc
     aacattctac tcctccaaaa aagaagagaa aggtagaaga ccccaaggac tttccttcag
     aattgctaag ttttttgagt catgctgtgt ttagtaatag aactcttgct tgctttgcta
     tttacaccac aaaggaaaaa gctgcactgc tatacaagaa aattatggaa aaatattctg
     taacctttat aagtaggcat aacagttata atcataacat actgtttttt cttactccac
     acaggcatag agtgtctgct attaataact atgctcaaaa attgtgtacc tttagctttt
     taatttgtaa aggggttaat aaggaatatt tgatgtatag tgccttgact agagatcata
     atcagccata ccacatttgt agaggtttta cttgctttaa aaaacctccc acacctcccc
     ctgaacctga aacataaaat gaatgcaatt gttgttgtta acttgtttat tgcagcttat
     aatggttaca aataaagcaa tagcatcaca aatttcacaa ataaagcatt tttttcactg
     cattctagtt gtggtttgtc caaactcatc aatgtatctt atcatgtctg gatccgtcga
     ccgatgccct tgagagcctt caacccagtc agctccttcc ggtgggcgcg gggcatgact
     atcgtcgccg cacttatgac tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca
     gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     ccgaaaagtg ccacctgacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg attagggtga
     tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggt
     ctattctttt gatttataag ggattttgcc gatttcggcc tattggttaa aaaatgagct
     gatttaacaa aaatttaacg cgaattttaa caaaatatta acgtttacaa tttcccattc
     gccattcagg ctgcgcaact gttgggaagg gcgatcggtg cgggcctctt cgctattacg
     ccagcccaag ctaccatgat aagtaagtaa tattaaggta cgtggaggtt ttacttgctt
     taaaaaacct cccacacctc cccctgaacc tgaaacataa aatgaatgca attgttgttg
     ttaacttgtt tattgcagct tataatggtt acaaataaag caatagcatc acaaatttca
     caaataaagc atttttttca ctgcattcta gttgtggttt gtccaaactc atcaatgtat
     cttatggtac tgtaactgag ctaacataa
//