back Return to this vector's summary.
ID   PGST       preliminary; circular DNA; SYN; 4905 BP.
XX
AC   X78316;
XX
DT   01-JUL-1994 (Rel. 9, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGST - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   1-4905
RC   pGST from BlueScribe M13+ & beta-globin gene & GST gene
RA   Chatton B., Bahr A., Acker J., Kedinger C.;
RT   "An eukaryotic GST-fusion vector for the study of protein
RT   interactions in vivo";
RL   Unpublished (1994).
XX
RN   [2]
RP   1-4905
RC   pGST
RA   Chatton B.;
RT   ;
RL   Submitted (18-MAR-1994) to the EMBL/GenBank/DDBJ databases by:
RL   Chatton B., LGME-CNRS, U184-INSERM, 11 rue Humann, 67085 Strasbourg,
RL   Cedex, FRANCE.
XX
CC   NCBI gi: 467622
CC   NM (pGST)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)
CC   CP ()
CC   FN (expression)(fusion)
CC   SE ()
CC   PA ()
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. BlueScribe M13+ 3204bp
FT                   2. pSV2-betaG, SV40 promoter/beta-globin gene
FT                   3. GST gene
FT                   -> pGST 4905bp"
FT   promoter        7..370
FT                   /note="PRO SV40"
FT   misc_feature    371..1010
FT                   /note="beta-globin IVS"
FT   promoter        1022..1040
FT                   /note="PRO phage T7"
FT   CDS             1049..1804
FT                   /note="glutathione S transferase fusion peptide"
FT   misc_feature    1049..1717
FT                   /note="glutathione S transferase sequence"
FT   misc_binding    1718..1729
FT                   /note="SIT factor X cleavage site"
FT   misc_binding    1730..1750
FT                   /note="SIT PKA site"
FT   misc_binding    1751..1783
FT                   /note="MCS"
FT   misc_feature    1784..1798
FT                   /note="five histidine (HIS)"
FT   misc_binding    1828..1889
FT                   /note="MCS"
FT   misc_feature    1890..4905
FT                   /note="from Bluescribe M13+"
XX
SQ   Sequence 4905 BP; 1290 A; 1130 C; 1155 G; 1330 T; 0 other;
     gtcgacttct gaggcggaaa gaaccagctg tggaatgtgt gtcagttagg gtgtggaaag
     tccccaggct ccccagcagg cagaagtatg caaagcatgc atctcaatta gtcagcaacc
     aggtgtggaa agtccccagg ctccccagca ggcagaagta tgcaaagcat gcatctcaat
     tagtcagcaa ccatagtccc gcccctaact ccgcccatcc cgcccctaac tccgcccagt
     tccgcccatt ctccgcccca tggctgacta atttttttta tttatgcaga ggccgaggcc
     gcctcggcct ctgagctatt ccagaagtag tgaggaggct tttttggagg cctaggcttt
     tgcaaaaagc tggatcgatc ctgagaactt cagggtgagt ttggggaccc ttgattgttc
     tttctttttc gctattgtaa aattcatgtt atatggaggg ggcaaagttt tcagggtgtt
     gtttagaatg ggaagatgtc ccttgtatca ccatggaccc tcatgataat tttgtttctt
     tcactttcta ctctgttgac aaccattgtc tcctcttatt ttcttttcat tttctgtaac
     tttttcgtta aactttagct tgcatttgta acgaattttt aaattcactt ttgtttattt
     gtcagattgt aagtactttc tctaatcact tttttttcaa ggcaatcagg gtatattata
     ttgtacttca gcacagtttt agagaacaat tgttataatt aaatgataag gtagaatatt
     tctgcatata aattctggct ggcgtggaaa tattcttatt ggtagaaaca actacatcct
     ggtcatcatc ctgcctttct ctttatggtt acaatgatat acactgtttg agatgaggat
     aaaatactct gagtccaaac cgggcccctc tgctaaccat gttcatgcct tcttcttttt
     cctacagctc ctgggcaacg tgctggttat tgtgctgtct catcattttg gcaaagaatt
     gtaatacgac tcactatagg gcgccgccat ggccaagtta cctatactag gttattggaa
     aattaagggc cttgtgcaac ccactcgact tcttttggaa tatcttgaag aaaaatatga
     agagcatttg tatgagcgcg atgaaggtga taaatggcga aacaaaaagt ttgaattggg
     tttggagttt cccaatcttc cttattatat tgatggtgat gttaaattaa cacagtctat
     ggccatcata cgttatatag ctgacaagca caacatgttg ggtggttgtc caaaagagcg
     tgcagagatt tcaatgcttg aaggagcggt tttggatatt agatacggtg tttcgagaat
     tgcatatagt aaagactttg aaactctcaa agttgatttt cttagcaagc tacctgaaat
     gctgaaaatg ttcgaagatc gtttatgtca taaaacatat ttaaatggtg atcatgtaac
     ccatcctgac ttcatgttgt atgacgctct tgatgttgtt ttatacatgg acccaatgtg
     cctggatgcg ttcccaaaat tagtttgttt taaaaaacgt attgaagcta tcccacaaat
     tgataagtac ttgaaatcca gcaagtatat agcatggcct ttgcagggct ggcaagccac
     gtttggtggt ggcgaccatc ctccaaaatc ggatctgatc gaaggtcgtg ggatcagaag
     agcatctgtg catatggata tcatcgcgac gttaactgct agccaccatc accatcacca
     gtaactagcc accatcacca ttaataagaa ttcggatcca agcttctcga ggcggccgcc
     ccgggctgca ggagctcggt accagatctt attaaagcag aacttgttta ttgcagctta
     taatggttac aaataaagca atagcatcac aaatttcaca aataaagcat ttttttcact
     gcattctagt tgtggtttgt ccaaactcat caatgtatct tatcatgtct ggtcgactct
     agactcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc
     ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg ataacgcagg
     aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct
     ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac gctcaagtca
     gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg gaagctccct
     cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct ttctcccttc
     gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg tgtaggtcgt
     tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct gcgccttatc
     cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac tggcagcagc
     cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt tcttgaagtg
     gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc tgctgaagcc
     agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca ccgctggtag
     cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga
     tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac gttaagggat
     tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt aaaaatgaag
     ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc aatgcttaat
     cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg cctgactccc
     cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg ctgcaatgat
     accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc cagccggaag
     ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta ttaattgttg
     ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg ttgccattgc
     tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct ccggttccca
     acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta gctccttcgg
     tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg ttatggcagc
     actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga ctggtgagta
     ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt gcccggcgtc
     aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca ttggaaaacg
     ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt cgatgtaacc
     cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt ctgggtgagc
     aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat
     actcatactc ttcctttttc aatattattg aagcatttat cagggttatt gtctcatgag
     cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc gcacatttcc
     ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa cctataaaaa
     taggcgtatc acgaggcccc tttcgtctcg cgcgtttcgg tgatgacggt gaaaacctct
     gacacatgca gctcccggag acggtcacag cttgtctgta agcggatgcc gggagcagac
     aagcccgtca gggcgcgtca gcgggtgttg gcgggtgtcg gggctggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc agatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggaaattgt aaacgttaat attttgttaa aattcgcgtt
     aaatttttgt taaatcagct cattttttaa ccaataggcc gaaatcggca aaatccctta
     taaatcaaaa gaatagaccg agatagggtt gagtgttgtt ccagtttgga acaagagtcc
     actattaaag aacgtggact ccaacgtcaa agggcgaaaa accgtctatc agggcgatgg
     cccactacgt gaaccatcac cctaatcaag ttttttgggg tcgaggtgcc gtaaagcact
     aaatcggaac cctaaaggga gcccccgatt tagagcttga cggggaaagc cggcgaacgt
     ggcgagaaag gaagggaaga aagcgaaagg agcgggcgct agggcgctgg caagtgtagc
     ggtcacgctg cgcgtaacca ccacacccgc cgcgcttaat gcgccgctac agggcgcgtc
     gcgccattcg ccattcaggc tacgcaactg ttgggaaggg cgatcggtgc gggcctcttc
     gctattacgc cagctggcga aggggggatg tgctgcaagg cgattaagtt gggtaacgcc
     agggttttcc cagtcacgac gttgtaaaac gacggccagt gaatt
//