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ID   PGUSN358S  preliminary; circular DNA; SYN; 4995 BP.
XX
AC   IG1080; S54410; M14641;
XX
DT   01-FEB-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phagemid vector pGUSN358-S - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   plasmid from pUC19 & oligo, translation initiator
RC   pRAJ275 from pRAJ255 & plasmid
RC   pBI221.1 from pBI121 & pUC19
RC   pBI121.2 from pRAJ275 & pBIN19
RC   pBI221.2 from pRAJ275 & pUC19
RA   Jefferson R.A.;
RT   "Assaying chimeric genes in plants: the GUS gene fusion system";
RL   Plant Mol. Biol. Rep. 5:387-405(1987).
XX
RN   [2]
RC   [from none]
RA   Jefferson R.A.;
RT   "The GUS reporter gene system";
RL   Nature 342:837-838(1989).
XX
RN   [3]
RC   pJF28 from pACYC177 & pTiT37, SalI fragment 10
RC   pJFdelta166 from M13mp7 & nopaline synthase gene & pBR322
RC   pJF28delta166 from pJF28 & pJFdelta166
RC   pBR325-B from pBR325
RC   pJFXhoX from pBR325-B & nopaline synthase gene & linker
RC   pJFXhoXdelta166 from pJFXhoX & pJF28delta166
RC   pAM1(B) from pAM1 & linker
RC   pJFneo+, pJFneo- from pJFXhoXdelta166 & pAM1(B)
RC   A208neo+ from pTiT37 & pRK290 & pJFneo+
RC   A208neo- from pTiT37 & pRK290 & pJFneo-
RA   Bevan M.W., Flavell R.B., Chilton M.D.;
RT   "A chimaeric antibiotic resistance gene as a selectable marker for
RT   plant cell transformation";
RL   Nature 304:184-187(1983).
XX
RN   [4]
RC   pBR325(EcoRI-13) from pBR325 & pTiT37, T-DNA
RC   pAM1 from pBR325 & Tn5, kan gene
RC   pAM2 from pAM1 & pBR325(EcoRI-13), pTiT37, T-DNA
RC   pAM3 from pAM2 & pRK290
RC   R751-pMG2 from R751
RC   pMoM from pAM3 & pTiT37
RC   MINI-Ti, MINI-Ti:Kpn11 from pMoM
RC   pRK290:EcoRI-1 from pRK290 & pTiT37, T-DNA
RC   MINI-Ti/pRK from pRK290:EcoRI-1 & MINI-Ti:Kpn11
RC   pAL4404 from pTiAch5
RA   De Framond A.J., Barton K.A., Chilton M.D.;
RT   "MINI-Ti: a new vector strategy for plant genetic engineering";
RL   Biotechnology 1:262-269(1983).
XX
CC   NM (pGUSN358-S)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (phagemid)
CC   SP (CLONTECH)
CC   HO (E.coli)
CC   CP ()
CC   FN (reporter)
CC   SE ()
CC   PA (pUC119)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. E. coli, GUS gene
FT                   -> pGUSN358-S 4995bp"
FT   misc_binding    0..0
FT                   /note="SIT HgiEII"
FT   rep_origin      0..0
FT                   /note="ORI bacteriophage M13 intergenic region
FT                   (M13IG)"
FT   misc_binding    0..0
FT                   /note="SIT NarI"
FT   CDS             complement(0..0)
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ)"
FT   misc_binding    0..0
FT                   /note="SIT BglI"
FT   misc_binding    0..0
FT                   /note="SIT FspI"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   misc_binding    0..0
FT                   /note="SIT EcoRI"
FT   misc_feature    0..0
FT                   /note="ATG"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-glucuronidase gene (GUS)"
FT   misc_binding    0..0
FT                   /note="SIT NcoI"
FT   misc_binding    0..0
FT                   /note="SIT SalI"
FT   misc_binding    0..0
FT                   /note="SIT PstI"
FT   misc_binding    0..0
FT                   /note="SIT SphI"
FT   misc_binding    0..0
FT                   /note="SIT HindIII"
FT   CDS             0..0
FT                   /note="REP E. coli lacI repressor gene"
FT   misc_binding    0..0
FT                   /note="SIT PvuII"
FT   misc_binding    0..0
FT                   /note="SIT AflIII"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    0..0
FT                   /note="SIT Cfr101"
FT   misc_binding    0..0
FT                   /note="SIT GsuI"
FT   misc_binding    0..0
FT                   /note="SIT BglII"
FT   misc_binding    0..0
FT                   /note="SIT AvaII"
FT   misc_binding    0..0
FT                   /note="SIT FspI"
FT   misc_binding    0..0
FT                   /note="SIT AvaII"
FT   misc_binding    0..0
FT                   /note="SIT PvuI"
FT   misc_binding    0..0
FT                   /note="SIT ScaI"
FT   misc_binding    0..0
FT                   /note="SIT XmnI"
FT   misc_binding    0..0
FT                   /note="SIT SspI"
FT   misc_binding    0..0
FT                   /note="SIT AatII"
FT   misc_binding    0..0
FT                   /note="SIT DraII"
XX
SQ   Sequence 4995 BP; 1231 A; 1257 C; 1296 G; 1211 T; 0 other;
     agcgcccaat acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcagctggc
     acgacaggtt tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttagc
     tcactcatta ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa
     ttgtgagcgg ataacaattt cacacaggaa acagctatga ccatgattac gccaagcttg
     catgcctgca ggtcgaccat ggtccgtcct gtagaaaccc caacccgtga aatcaaaaaa
     ctcgacggcc tgtgggcatt cagtctggat cgcgaaaact gtggaattga tcagcgttgg
     tgggaaagcg cgttacaaga aagccgggca attgctgtgc caggcagttt taacgatcag
     ttcgccgatg cagatattcg taattatgcg ggcaacgtct ggtatcagcg cgaagtcttt
     ataccgaaag gttgggcagg ccagcgtatc gtgctgcgtt tcgatgcggt cactcattac
     ggcaaagtgt gggtcaataa tcaggaagtg atggagcatc agggcggcta tacgccattt
     gaagccgatg tcacgccgta tgttattgcc gggaaaagtg tacgtatcac cgtttgtgtg
     aacaacgaac tgaactggca gactatcccg ccgggaatgg tgattaccga cgaaaacggc
     aagaaaaagc agtcttactt ccatgatttc tttaactatg ccggaatcca tcgcagcgta
     atgctctaca ccacgccgaa cacctgggtg gacgatatca ccgtggtgac gcatgtcgcg
     caagactgta accacgcgtc tgttgactgg caggtggtgg ccaatggtga tgtcagcgtt
     gaactgcgtg atgcggatca acaggtggtt gcaactggac aaggcactag cgggactttg
     caagtggtga atccgcacct ctggcaaccg ggtgaaggtt atctctatga actgtgcgtc
     acagccaaaa gccagacaga gtgtgatatc tacccgcttc gcgtcggcat ccggtcagtg
     gcagtgaagg gccaacagtt cctgattaac cacaaaccgt tctactttac tggctttggt
     cgtcatgaag atgcggactt acgtggcaaa ggattcgata acgtgctgat ggtgcacgac
     cacgcattaa tggactggat tggggccaac tcctaccgta cctcgcatta cccttacgct
     gaagagatgc tcgactgggc agatgaacat ggcatcgtgg tgattgatga aactgctgct
     gtcggctttt cgctctcttt aggcattggt ttcgaagcgg gcaacaagcc gaaagaactg
     tacagcgaag aggcagtcaa cggggaaact cagcaagcgc acttacaggc gattaaagag
     ctgatagcgc gtgacaaaaa ccacccaagc gtggtgatgt ggagtattgc caacgaaccg
     gatacccgtc cgcaaggtgc acgggaatat ttcgcgccac tggcggaagc aacgcgtaaa
     ctcgacccga cgcgtccgat cacctgcgtc aatgtaatgt tctgcgacgc tcacaccgat
     accatcagcg atctctttga tgtgctgtgc ctgaaccgtt attacggatg gtatgtccaa
     agcggcgatt tggaaacggc agagaaggta ctggaaaaag aacttctggc ctggcaggag
     aaactgcatc agccgattat catcaccgaa tacggcgtgg atacgttagc cgggctgcac
     tcaatgtaca ccgacatgtg gagtgaagag tatcagtgtg catggctgga tatgtatcac
     cgcgtctttg atcgcgtcag cgccgtcgtc ggtgaacagg tatggaattt cgccgatttt
     gcgacctcgc aaggcatatt gcgcgttggc ggtaacaaga aagggatctt cactcgcgac
     cgcaaaccga agtcggcggc ttttctgctg caaaaacgct ggactggcat gaacttcggt
     gaaaaaccgc agcagggagg caaacaatga atcaacaact ctcctggcgc accatcgtcg
     gctacagcct cggtggggaa ttcactggcc gtcgttttac aacgtcgtga ctgggaaaac
     cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
     agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa tggcgaatgg
     cgcctgatgc ggtattttct ccttacgcat ctgtgcggta tttcacaccg catacgtcaa
     agcaaccata gtacgcgccc tgtagcggcg cattaagcgc ggcgggtgtg gtggttacgc
     gcagcgtgac cgctacactt gccagcgccc tagcgcccgc tcctttcgct ttcttccctt
     cctttctcgc cacgttcgcc ggctttcccc gtcaagctct aaatcggggg ctccctttag
     ggttccgatt tagtgcttta cggcacctcg accccaaaaa acttgatttg ggtgatggtt
     cacgtagtgg gccatcgccc tgatagacgg tttttcgccc tttgacgttg gagtccacgt
     tctttaatag tggactcttg ttccaaactg gaacaacact caaccctatc tcgggctatt
     cttttgattt ataagggatt ttgccgattt cggcctattg gttaaaaaat gagctgattt
     aacaaaaatt taacgcgaat tttaacaaaa tattaacgtt tacaatttta tggtgcactc
     tcagtacaat ctgctctgat gccgcatagt taagccagcc ccgacacccg ccaacacccg
     ctgacgcgcc ctgacgggct tgtctgctcc cggcatccgc ttacagacaa gctgtgaccg
     tctccgggag ctgcatgtgt cagaggtttt caccgtcatc accgaaacgc gcgagacgaa
     agggcctcgt gatacgccta tttttatagg ttaatgtcat gataataatg gtttcttaga
     cgtcaggtgg cacttttcgg ggaaatgtgc gcggaacccc tatttgttta tttttctaaa
     tacattcaaa tatgtatccg ctcatgagac aataaccctg ataaatgctt caataatatt
     gaaaaaggaa gagtatgagt attcaacatt tccgtgtcgc ccttattccc ttttttgcgg
     cattttgcct tcctgttttt gctcacccag aaacgctggt gaaagtaaaa gatgctgaag
     atcagttggg tgcacgagtg ggttacatcg aactggatct caacagcggt aagatccttg
     agagttttcg ccccgaagaa cgttttccaa tgatgagcac ttttaaagtt ctgctatgtg
     gcgcggtatt atcccgtatt gacgccgggc aagagcaact cggtcgccgc atacactatt
     ctcagaatga cttggttgag tactcaccag tcacagaaaa gcatcttacg gatggcatga
     cagtaagaga attatgcagt gctgccataa ccatgagtga taacactgcg gccaacttac
     ttctgacaac gatcggagga ccgaaggagc taaccgcttt tttgcacaac atgggggatc
     atgtaactcg ccttgatcgt tgggaaccgg agctgaatga agccatacca aacgacgagc
     gtgacaccac gatgcctgta gcaatggcaa caacgttgcg caaactatta actggcgaac
     tacttactct agcttcccgg caacaattaa tagactggat ggaggcggat aaagttgcag
     gaccacttct gcgctcggcc cttccggctg gctggtttat tgctgataaa tctggagccg
     gtgagcgtgg gtctcgcggt atcattgcag cactggggcc agatggtaag ccctcccgta
     tcgtagttat ctacacgacg gggagtcagg caactatgga tgaacgaaat agacagatcg
     ctgagatagg tgcctcactg attaagcatt ggtaactgtc agaccaagtt tactcatata
     tactttagat tgatttaaaa cttcattttt aatttaaaag gatctaggtg aagatccttt
     ttgataatct catgaccaaa atcccttaac gtgagttttc gttccactga gcgtcagacc
     ccgtagaaaa gatcaaagga tcttcttgag atcctttttt tctgcgcgta atctgctgct
     tgcaaacaaa aaaaccaccg ctaccagcgg tggtttgttt gccggatcaa gagctaccaa
     ctctttttcc gaaggtaact ggcttcagca gagcgcagat accaaatact gtccttctag
     tgtagccgta gttaggccac cacttcaaga actctgtagc accgcctaca tacctcgctc
     tgctaatcct gttaccagtg gctgctgcca gtggcgataa gtcgtgtctt accgggttgg
     actcaagacg atagttaccg gataaggcgc agcggtcggg ctgaacgggg ggttcgtgca
     cacagcccag cttggagcga acgacctaca ccgaactgag atacctacag cgtgagctat
     gagaaagcgc cacgcttccc gaagggagaa aggcggacag gtatccggta agcggcaggg
     tcggaacagg agagcgcacg agggagcttc cagggggaaa cgcctggtat ctttatagtc
     ctgtcgggtt tcgccacctc tgacttgagc gtcgattttt gtgatgctcg tcaggggggc
     ggagcctatg gaaaaacgcc agcaacgcgg cctttttacg gttcctggcc ttttgctggc
     cttttgctca catgttcttt cctgcgttat cccctgattc tgtggataac cgtattaccg
     cctttgagtg agctgatacc gctcgccgca gccgaacgac cgagcgcagc gagtcagtga
     gcgaggaagc ggaag
//