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ID   PGV451     preliminary; circular DNA; SYN; 1579 BP.
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AC   IG1390;
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DT   01-OCT-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pGV451 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pGV451 from pBR325 & HyRep2 ori
RC   pGV462, pGV463 from pGV451 & linker
RA   Volckaert G.;
RT   "A systematic approach to chemical DNA sequencing by subcloning in
RT   pGV451 and derived vectors";
RL   Meth. Enzymol. 155:231-250(1987).
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CC   NM (pGV451)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli HB101)
CC   CP ()
CC   FN (cloning general)
CC   SE ()
CC   PA (pBR322)(pBR325)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR325 TaqI-TaqI 773bp 4535..5308, cat gene
FT                   \ #V00622
FT                   2. E. coli 806bp, HyRep2 ori
FT                   -> pGV451 1579bp"
FT   misc_binding    20..74
FT                   /note="MCS unique HindIII-BstEII-Tth111I-SmaI-BamHI-
FT                   SalI-HincII-AccI-PstI-XmnI-BglII"
FT   misc_binding    20..20
FT                   /note="SIT unique HindIII"
FT   misc_binding    29..29
FT                   /note="SIT unique BstEII"
FT   misc_binding    57..57
FT                   /note="SIT unique AvaI or SmaI"
FT   misc_binding    62..62
FT                   /note="SIT unique BamHI"
FT   misc_binding    68..68
FT                   /note="SIT unique AccI or SalI or HincII"
FT   misc_binding    74..74
FT                   /note="SIT unique PstI"
FT   promoter        0..0
FT                   /note="PRO E. coli promoter P3"
FT   CDS             0..0
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr)"
FT   misc_binding    228..228
FT                   /note="SIT unique AatII"
FT   misc_binding    472..472
FT                   /note="SIT unique PvuII"
FT   misc_binding    574..574
FT                   /note="SIT unique EcoRI"
FT   misc_binding    839..839
FT                   /note="SIT unique BalI"
FT   misc_binding    875..875
FT                   /note="SIT unique NcoI"
FT   misc_binding    989..989
FT                   /note="SIT unique ScaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   misc_binding    1040..1040
FT                   /note="SIT unique BanI"
FT   misc_binding    1141..1141
FT                   /note="SIT unique MaeI"
FT   misc_binding    1320..1320
FT                   /note="SIT unique NgiAI"
FT   misc_binding    1390..1390
FT                   /note="SIT unique HaeII"
FT   misc_binding    1533..1533
FT                   /note="SIT unique HgaI"
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SQ   Sequence 1579 BP; 410 A; 360 C; 403 G; 406 T; 0 other;
     acgccagcaa cgcggcccga agcttatcgg tgaccctgac taagtcgagc ccaattcccg
     gggatccgtc gacctgcagc caagctgggc tcgactcagt cagggcgatg ataagctgtc
     aaacatgaga attgaagatc ttcgaagatc ttcaattctt gaagacgaaa gggcctcgtg
     atacgcctat ttttataggt taatgtcatg ataataatgg tttcttagac gtcaggtggc
     acttttcggg gaaatgtgcg cggaacccct atttgtttat ttttctaaat acattcaaat
     atgtatccgc tcatgagaca ataaccctga tcgagatttt caggagctaa ggaagctaaa
     atggagaaaa aaatcactgg atataccacc gttgatatat cccaatggca tcgtaaagaa
     cattttgagg catttcagtc agttgctcaa tgtacctata accagaccgt tcagctggat
     attacggcct ttttaaagac cgtaaagaaa aataagcaca agttttatcc ggcctttatt
     cacattcttg cccgcctgat gaatgctcat ccggaattcc gtatggcaat gaaagacggt
     gagctggtga tatgggatag tgttcaccct tgttacaccg ttttccatga gcaaactgaa
     acgttttcat cgctctggag tgaataccac gacgatttcc ggcagtttct acacatatat
     tcgcaagatg tggcgtgtta cggtgaaaac ctggcctatt tccctaaagg gtttattgag
     aatatgtttt tcgtctcagc caatccctgg gtgagtttca ccagttttga tttaaacgtg
     gccaatatgg acaacttctt cgcccccgtt ttcaccatgg gcaaatatta tacgcaaggc
     gacaaggtgc tgatgccgct ggcgattcag gttcatcatg ccgtttgtga tggcttccat
     gtcggcagaa tgcttaatga attacaacag tactgcgatg agtggcaggg cggggcgtaa
     tttttttaag gcagttattg gtgcccttaa acgcctggtg ctacgcctga ataagtgata
     ataagcggat gaatggcaga aactggcttc agcagagcgc agataccaaa tactgtcctt
     ctagtgtagc cgtagttagg ccaccacttc aagaactctg tagcaccgcc tacatacctc
     gctctgctaa tcctgttacc agtggctgct gccagtggcg ataagtcgtg tcttaccggg
     ttggactcaa gacgatagtt accggataag gcgcagcggt cgggctgaac ggggggttcg
     tgcacacagc ccagcttgga gcgaacgacc tacaccgaac tgagatacct acagcgtgag
     cattgagaaa gcgccacgct tcccgaaggg agaaaggcgg acaggtatcc ggtaagcggc
     agggtcggaa caggagagcg cacgagggag cttccagggg gaaacgcctg gtatctttat
     agtcctgtcg ggtttcgcca cctctgactt gagcgtcgat ttttgtgatg ctcgtcaggg
     gggcggagcc tatggaaaa
//