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ID   PGW10      preliminary; circular DNA; SYN; 11594 BP.
XX
AC   ATCC40167;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGW10 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGW10 from pBR322 & lambda, cI857 gene/pL/pR
RA   ;
RT   ;
RL   Unpublished (1991).
RL   U.S. Patent No. 5,004,691 dated Apr. 2, 1991.
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Expression vector encoding cI857. (ATCC staff)
CC   The plasmid contains the following restriction sites (starting at the
CC   EcoRI site proceeding through the pBR322 sequences):HindIII--29, 5192,
CC   9997, 10561, 10686; BamHI--375, 7601; SalI--651; PvuII--2066;
CC   PvuI--3735, 8889; EcoRI--1, 4361. (personal communication)
CC   The order of the major features in this plasmid is: EcoRI site - tetR
CC   - pMB1 ori - bla - bacteriophage lambda sequences Q, N, PR, rex,
CC   cI857, PR, Cr, O. (personal communication)
CC   The 4.3 kb BamHI fragment contains the PL and PR promoters, the cI857
CC   gene, and 375 bp of pBR322 sequence.
CC   Distributed in aliquots of 2 ug (40 ng/ul). (ATCC staff)
CC   The insert contains the following restriction sites (approximate kb
CC   from the 5' HindIII site):BamHI--2.5; NsiI--2.9, 9.5; ScaI--7.9;
CC   EcoRI--8.0. (personal communication)
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--5.2, 4.8, 0.85, 0.5; BamHI--6.8, 4.3; EcoRI--6.8, 4.3;
CC   PvuII--11.6. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pGW10)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (depositor-supplied DNA)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI 4361bp 376..376
FT                   2. lambda BamHI-BamHI 7233bp 34500..41733,
FT                   \ cI857 gene/pL/pR
FT                   \ BamHI = 5506 22347 27973 34500 41733
FT                   -> pGW10 11594bp [11561bp]"
FT   -               1..375
FT                   /note="pBR322 1..375 375bp
FT                   BamHI = G^GATCC"
FT   -               376..7608
FT                   /note="lambda 34500..41732 7233bp complement
FT                   BamHI = G^GATCC"
FT   -               7609..11594
FT                   /note="pBR322 376..4361 3986bp"
FT   misc_binding    0..0
FT                   /note="SIT unique PvuII-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pL"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pR"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   CDS             0..0
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
XX
SQ   Sequence 11594 BP; 2864 A; 2935 C; 2730 G; 3065 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtgccgtg atgacctcat taaaaacacg ctgcaatccc tcctcatctt
     tgcaggcaag tccgattttt tgcgttgatt ttttaatgca gaatatgcag ttaccgagat
     gttccggtat ttgcaaatcg aatggttgtt gcttccacca tgcgaggata tcttccttct
     caaagtctga cagttcagca agatatctga ttccaggctt tggctttagc cgcttcggtt
     catcagctct gatgccaatc cacgtggtgt aattccctcg cccgaaatgg tcatcacagt
     atttggtgaa gggaacgagt tttaatctgt cagtgcagaa cgcgccgccg acgtatggag
     tgccatattt ctttaccata tcgataaatg gcttcagaac aggcattcgc gtctgaatat
     cctttggttc ccataccgta taaccatttg gctgtccaag ctccgggttg atatcaacct
     gcaatacggt gagcggtata tcccagaact tcacaacttc cctgacaaac cgatatgtca
     ttggatgttc acaacctgta tccatgaaaa cgtaatgcac gtctttacct gcccgtcgct
     tttgctccat tagccagagc aaatatgctg acgtcctgcc accggagaaa ctaacgacat
     ttatcatgca gccctgtctc cccatctcgc tttccactcc agagccagtc tcgcttcgtc
     tgaccactta acgccacgct ctgtaccgaa tgcctgtata agctctaata gctccgcaaa
     ttcgcctaca cgcatcctgc tggttgactg gcctattacc acaaagccat tcccggcaag
     gttaggaaca acatcctgct gctttaatgc tgcggtaaac acacacttcc agctttctgc
     atccagccag cgaccatgcc attcaacctg acgagagacg tcacctaagc aggcccatag
     cttcctgttt tggtctaagc tgcggttgcg ttcctgaatg gttactacga ttggtttggt
     tgggtctgga aggatttgct gtactgcgtg aatagcgttt tgctgatgtg ctggagatcg
     aatttcaaag gttagttttt tcatgacttc cctctccccc aaataaaaag gcctgcgatt
     accagcaggc ctgttattag ctcagtaatg tagatggtca tcttttaact ccatataccg
     ccaatacccg tttcatcgcg gcactctggc gacactcctt aaaaaccagg ttcgtgctca
     tctttccttc ccgttcttcc ctggtagcaa accggtaata caccgttcgc cagaccttac
     cttcgataac cagaagacct gcccgtgcca ttttagccgc ggcctgattt atgctggtta
     ctgttgcgcc tgttagcgcg gcaacgtccg gcgcacagaa gctattatgc gtccccaggt
     aatgaataat tgcctctttg cccgtcatac acttgctcct ttcagtccga acttagcttt
     gatttctgcg atcttcgcca gagcctgtgc acgatttaga ggtctaccgc ccatgacagg
     aagttgtttt actggttcag ggatcgcctc accacggtta attctcgcag tcatatggac
     aagctcatct gcggccttac ggcgtaattc cgcatcagta agcgcattgg cccgcatgtt
     ctgatacagg ttggtaacca gccagtagtg cgcgtttgat ttccacggat aagactccgc
     atccggatac aggcctcgct tccggcaata ctcgtaaacc atatcaacca gctcgctgac
     gtttggcagt ccggcggtaa cggatgcttc ttcccggcac catgcaacaa actgcccggg
     tgatggcaga aatggtcgat tctgccgacg ggctacgcgc attcctgcgt taacctgttc
     catcgtggtg atcccgtttt cccgaaaagc cagaacccac tggcgacgga tttcgttcac
     ttcgttctgg tcacggttag ccaggctcgc cgggaaagtt gccagtaact ggctgaacac
     accgttgatg atctgcgcta cctgctgtac ctgcggcttt tcgtcgtact gttccggcat
     gttgttggcg atccgacgca tctgctcacg gtcaaagtta accatctgtg cggcgatgtt
     tttcatagat ccaccccgta aatccagtct gtgtttgtca ggtcgagttt tggtttgctg
     gctgtcacgc ctgcctgttg cttgttacgg ttgatttcga gttgggtcca cttatcgcgg
     agtttggccg ggctcagcac gttaccggac cagaagttgt cctggcatgc ccagcggaac
     agcacacaca tgtcgcggtg gttacgtccg tcacgttcac gcatcaggcg gatatcgtta
     gcccacccag caaaattcgg ttttctggct gatggtgcga tagtcttcac catgtcaaac
     atccactctg cggcggtcag gtcttctgct gtcccccact tgctgccgct ctgaattgca
     gcatccggtt tcaccacaga aaggtcgttt tctggctggt cagaggattc gccagaattc
     tctgacgaat aatcttttct tttttctttt gtaatagtgt cttttgtgtc cccctgtttt
     gagggatagc aatcccccaa tttgagggat gttttatccc tcgttttagg ggattttccc
     tcgttttgag ggatgcacca ttctgagatg tttttatttg gtccaaacat gccgccttgc
     tgcttgataa tattcattct gacgagttct aacttggctt cattgcaccg tttgacaggt
     aactttgtaa tctcgctaag ttgagaatcg gtgattctgt ccattggttt attccaccca
     taggttttac gcagaatggc aagcagcact ttaaactgtc gcttggtcag atctgcgccc
     gaataagcct caagcagcat atttgatagt ctggcgtaac catcatcgag atctgccaca
     ttacgctcct gtccggcaaa gttacctctg ccgaagttga gtatttttgc tgtatttgtc
     ataatgactc ctgttgatag atccagtaat gacctcagaa ctccatctgg atttgttcag
     aacgctcggt tgccgccggg cgttttttat tggtgagaat cgcagcaact tgtcgcgcca
     atcgagccat gtcgtcgtca acgacccccc attcaagaac agcaagcagc attgagaact
     ttggaatcca gtccctcttc cacctgctga tctgcgactt atcaacgccc acagcttccg
     ctgtcttctc agttccaagc attgcgattt tgttaagcaa cgcactctcg attcgtagag
     cctcgttgcg tttgtttgca cgaaccatat gtaagtattt ccttagataa caattgattg
     aatgtatgca aataaatgca tacaccatag gtgtggttta atttgatgcc ctttttcagg
     gctggaatgt gtaagagcgg ggttatttat gctgttgttt ttttgttact cgggaagggc
     tttacctctt ccgcataaac gcttccatca gcgtttatag ttaaaaaaat ctttcggcct
     gcatgaatgg ccttgttgat cgcgctttga tatacgccga gatctttagc tgtcttggtt
     tgcccaaagc gcattgcata atctttcagg gttatgcgtt gttccataca acctccttag
     tacatgcaac cattatcacc gccagaggta aaatagtcaa cacgcacggt gttagatatt
     tatcccttgc ggtgatagat ttaacgtatg agcacaaaaa agaaaccatt aacacaagag
     cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc
     ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt tggtgcttta
     tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa
     gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat gtatgaagcg
     gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc tcatgttcag
     gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga gagatgggta
     agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg taattccatg
     accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct cgttgaccct
     gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga tgagtttacc
     ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa cccacagtac
     ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc tagtcagtgg
     cctgaagaga cgtttggctg atcggcaagg tgttctggtc ggcgcatagc tgataacaat
     tgagcaagaa tcttcatcga attaggggaa ttttcactcc cctcagaaca taacatagta
     aatggattga attatgaaga atggttttta tgcgacttac cgcagcaaaa ataaagggaa
     agataagcgc tcaataaacc tgtctgtttt ccttaattct ctgctggctg ataatcatca
     cctgcaggtt ggctccaatt atttgtatat tcataaaatc gatggaaaaa cttttctctt
     taccaaaaca aatgacaaga gtctggttca gaagataaat cgctctaaag cttcagttga
     agatattaag aacagcctcg cagatgacga atcattggga ttcccatctt ttttgtttgt
     tgaaggcgac accattggtt ttgccagaac tgttttcggg ccgaccacat ccgatctgac
     agatttttta atcgggaaag gaatgtcatt aagcagtgga gagcgcgttc agatagagcc
     actgatgagg ggaaccacca aagacgatgt tatgcatatg catttcatcg gccgaacaac
     ggtgaaggta gaagccaagc tacctgtatt tggcgatata ttaaaggtct taggggcaac
     agatattgaa ggggagcttt ttgactcatt ggatatagtc attaagccaa aatttaaaag
     ggatataaaa aaggttgcca aggatattat ttttaacccg tcacctcaat tttcagacat
     tagcctgcgg gcaaaagatg aggccggaga tattttaaca gaacattatc tatcagaaaa
     aggccatctc tcagcgcctc tgaacaaggt caccaatgct gagatagctg aagagatggc
     atattgctac gcaagaatga aaagtgatat actggaatgt tttaaaaggc aggtgggcaa
     agttaaggat taattatcag gagtaattat gcggaacaga atcatgcctg gtgtttacat
     agtaataatt ccttacgtta tcgtaagcat ttgctatctc cttttccgcc actacattcc
     tggtgtttct ttttcagctc atagagatgg tcttggggcg acattgtcat catatgcagg
     aaccatgatt gcaatcctga ttgctgcctt gacgtttcta atcggaagca gaacgcgccg
     actggccaag attagagagt atgggtatat gacatcggta gttattgtct atgcccttag
     ttttgttgag cttggagctt tgtttttctg cgggttattg cttctttcca gcataagcgg
     ctacatgata cccactatcg ccatcggcat tgcctctgca tcgttcattc atatatgcat
     ccttgttttc caactatata atttgaccag agaacaagaa taacccggcc tcagcgccgg
     gttttctttg cctcacgatc gcccccaaaa cacataacca attgtattta ttgaaaaata
     aatagataca actcactaaa catagcaatt cagatctctc acctaccaaa caatgccccc
     ctgcaaaaaa taaattcata taaaaaacat acagataacc atctgcggtg ataaattatc
     tctggcggtg ttgacataaa taccactggc ggtgatactg agcacatcag caggacgcac
     tgaccaccat gaaggtgacg ctcttaaaaa ttaagccctg aagaagggca gcattcaaag
     cagaaggctt tggggtgtgt gatacgaaac gaagcattgg ccgtaagtgc gattccggat
     tagctgccaa tgtgccaatc gcggggggtt ttcgttcagg actacaactg ccacacacca
     ccaaagctaa ctgacaggag aatccagatg gatgcacaaa cacgccgccg cgaacgtcgc
     gcagagaaac aggctcaatg gaaagcagca aatcccctgt tggttggggt aagcgcaaaa
     ccagttaacc gccctattct ctcgctgaat cgcaaaccga aatcacgagt agaaagcgca
     ctaaatccga tagaccttac agtgctggct gaataccaca aacagattga aagcaacctg
     caacgtattg agcgcaagaa tcagcgcaca tggtacagca agcctggcga acgcggcata
     acatgcagtg gacgccagaa aattaaggga aaatcgattc ctcttatcta gttacttaga
     tattggcctt ggctttatct caatattata tggatcatag ctggcaacta attcagtcca
     gtaaatatcc tcaataggga ataatatatg ctttccattc catcgggaaa aagttttgtt
     caacacacca agctcaatca actcactaat gtatgggaat tgttttgatg taaccacata
     cttcctgcct tcattaaggg ctgcgcacaa aaccatagat tgctcttctg taaggttttg
     aattactgat cgcactttat cgttttgcat cttaatgcgt tttcttagct taaatcgctt
     atatctggcg ctggcaatag ctgataatcg atgcacatta attgctagcg aaaatgcaag
     agcaaagacg aaaacatgcc acacatgagg aataccgatt ctctcattaa catattcagg
     ccagttatct gggcttaaaa gcagaagtcc aacccagata acgatcatat acatggttct
     ctccagaggt tcattactga acactcgtcc gagaataacg agtggatcga tcctctacgc
     cggacgcatc gtggccggca tcaccggcgc cacaggtgcg gttgctggcg cctatatcgc
     cgacatcacc gatggggaag atcgggctcg ccacttcggg ctcatgagcg cttgtttcgg
     cgtgggtatg gtggcaggcc ccgtggccgg gggactgttg ggcgccatct ccttgcatgc
     accattcctt gcggcggcgg tgctcaacgg cctcaaccta ctactgggct gcttcctaat
     gcaggagtcg cataagggag agcgtcgacc gatgcccttg agagccttca acccagtcag
     ctccttccgg tgggcgcggg gcatgactat cgtcgccgca cttatgactg tcttctttat
     catgcaactc gtaggacagg tgccggcagc gctctgggtc attttcggcg aggaccgctt
     tcgctggagc gcgacgatga tcggcctgtc gcttgcggta ttcggaatct tgcacgccct
     cgctcaagcc ttcgtcactg gtcccgccac caaacgtttc ggcgagaagc aggccattat
     cgccggcatg gcggccgacg cgctgggcta cgtcttgctg gcgttcgcga cgcgaggctg
     gatggccttc cccattatga ttcttctcgc ttccggcggc atcgggatgc ccgcgttgca
     ggccatgctg tccaggcagg tagatgacga ccatcaggga cagcttcaag gatcgctcgc
     ggctcttacc agcctaactt cgatcactgg accgctgatc gtcacggcga tttatgccgc
     ctcggcgagc acatggaacg ggttggcatg gattgtaggc gccgccctat accttgtctg
     cctccccgcg ttgcgtcgcg gtgcatggag ccgggccacc tcgacctgaa tggaagccgg
     cggcacctcg ctaacggatt caccactcca agaattggag ccaatcaatt cttgcggaga
     actgtgaatg cgcaaaccaa cccttggcag aacatatcca tcgcgtccgc catctccagc
     agccgcacgc ggcgcatctc gggcagcgtt gggtcctggc cacgggtgcg catgatcgtg
     ctcctgtcgt tgaggacccg gctaggctgg cggggttgcc ttactggtta gcagaatgaa
     tcaccgatac gcgagcgaac gtgaagcgac tgctgctgca aaacgtctgc gacctgagca
     acaacatgaa tggtcttcgg tttccgtgtt tcgtaaagtc tggaaacgcg gaagtcagcg
     ccctgcacca ttatgttccg gatctgcatc gcaggatgct gctggctacc ctgtggaaca
     cctacatctg tattaacgaa gcgctggcat tgaccctgag tgatttttct ctggtcccgc
     cgcatccata ccgccagttg tttaccctca caacgttcca gtaaccgggc atgttcatca
     tcagtaaccc gtatcgtgag catcctctct cgtttcatcg gtatcattac ccccatgaac
     agaaatcccc cttacacgga ggcatcagtg accaaacagg aaaaaaccgc ccttaacatg
     gcccgcttta tcagaagcca gacattaacg cttctggaga aactcaacga gctggacgcg
     gatgaacagg cagacatctg tgaatcgctt cacgaccacg ctgatgagct ttaccgcagc
     tgcctcgcgc gtttcggtga tgacggtgaa aacctctgac acatgcagct cccggagacg
     gtcacagctt gtctgtaagc ggatgccggg agcagacaag cccgtcaggg cgcgtcagcg
     ggtgttggcg ggtgtcgggg cgcagccatg acccagtcac gtagcgatag cggagtgtat
     actggcttaa ctatgcggca tcagagcaga ttgtactgag agtgcaccat atgcggtgtg
     aaataccgca cagatgcgta aggagaaaat accgcatcag gcgctcttcc gcttcctcgc
     tcactgactc gctgcgctcg gtcgttcggc tgcggcgagc ggtatcagct cactcaaagg
     cggtaatacg gttatccaca gaatcagggg ataacgcagg aaagaacatg tgagcaaaag
     gccagcaaaa ggccaggaac cgtaaaaagg ccgcgttgct ggcgtttttc cataggctcc
     gcccccctga cgagcatcac aaaaatcgac gctcaagtca gaggtggcga aacccgacag
     gactataaag ataccaggcg tttccccctg gaagctccct cgtgcgctct cctgttccga
     ccctgccgct taccggatac ctgtccgcct ttctcccttc gggaagcgtg gcgctttctc
     atagctcacg ctgtaggtat ctcagttcgg tgtaggtcgt tcgctccaag ctgggctgtg
     tgcacgaacc ccccgttcag cccgaccgct gcgccttatc cggtaactat cgtcttgagt
     ccaacccggt aagacacgac ttatcgccac tggcagcagc cactggtaac aggattagca
     gagcgaggta tgtaggcggt gctacagagt tcttgaagtg gtggcctaac tacggctaca
     ctagaaggac agtatttggt atctgcgctc tgctgaagcc agttaccttc ggaaaaagag
     ttggtagctc ttgatccggc aaacaaacca ccgctggtag cggtggtttt tttgtttgca
     agcagcagat tacgcgcaga aaaaaaggat ctcaagaaga tcctttgatc ttttctacgg
     ggtctgacgc tcagtggaac gaaaactcac gttaagggat tttggtcatg agattatcaa
     aaaggatctt cacctagatc cttttaaatt aaaaatgaag ttttaaatca atctaaagta
     tatatgagta aacttggtct gacagttacc aatgcttaat cagtgaggca cctatctcag
     cgatctgtct atttcgttca tccatagttg cctgactccc cgtcgtgtag ataactacga
     tacgggaggg cttaccatct ggccccagtg ctgcaatgat accgcgagac ccacgctcac
     cggctccaga tttatcagca ataaaccagc cagccggaag ggccgagcgc agaagtggtc
     ctgcaacttt atccgcctcc atccagtcta ttaattgttg ccgggaagct agagtaagta
     gttcgccagt taatagtttg cgcaacgttg ttgccattgc tgcaggcatc gtggtgtcac
     gctcgtcgtt tggtatggct tcattcagct ccggttccca acgatcaagg cgagttacat
     gatcccccat gttgtgcaaa aaagcggtta gctccttcgg tcctccgatc gttgtcagaa
     gtaagttggc cgcagtgtta tcactcatgg ttatggcagc actgcataat tctcttactg
     tcatgccatc cgtaagatgc ttttctgtga ctggtgagta ctcaaccaag tcattctgag
     aatagtgtat gcggcgaccg agttgctctt gcccggcgtc aacacgggat aataccgcgc
     cacatagcag aactttaaaa gtgctcatca ttggaaaacg ttcttcgggg cgaaaactct
     caaggatctt accgctgttg agatccagtt cgatgtaacc cactcgtgca cccaactgat
     cttcagcatc ttttactttc accagcgttt ctgggtgagc aaaaacagga aggcaaaatg
     ccgcaaaaaa gggaataagg gcgacacgga aatgttgaat actcatactc ttcctttttc
     aatattattg aagcatttat cagggttatt gtctcatgag cggatacata tttgaatgta
     tttagaaaaa taaacaaata ggggttccgc gcacatttcc ccgaaaagtg ccacctgacg
     tctaagaaac cattattatc atgacattaa cctataaaaa taggcgtatc acgaggccct
     ttcgtcttca agaa
//