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ID   PGW7       preliminary; circular DNA; SYN; 8002 BP.
XX
AC   ATCC40166;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pGW7 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pGW7 from pBR322 & lambda, N gene/cI gene/pL/pR
RA   ;
RT   ;
RL   Unpublished (1993).
RL   U.S. Patent No. 5,180,673 dated Jan. 19, 1993.
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Restriction digests of the clone give the following sizes (kb):
CC   EcoRI--8.0; BamHI--8.0; EcoRI/BamHI--4.0 (doublet). (ATCC staff)
CC   Expression vector permitting thermal regulation of expression from the
CC   PL promoter. [1]
CC   Constructed from a BamHI/EcoRI fragment of pBR322 and a 3.96 kb
CC   BamHI/EcoRI fragment from bacteriophage lambda, containing N, rexB,
CC   rexA, cI and modified by deleting a 711 bp BglII fragment containing
CC   cII and portions of the cro and O genes. [1]
CC   The order of the major features in the plasmid is:  EcoRI - partial O
CC   - partial cro - lambda R promoter - lambda M promoter - cIts - rexA -
CC   rexB - lambda L promoter - HpaI / N - BamHI - SphI - SalI - pMB1 ori -
CC   ampR. [1]
CC   The vector contains the following restriction sites (bp from the EcoRI
CC   site):  BglII--352, 2744; HindIII--871, 996, 1560; PstI--1454, 7190;
CC   HpaI--3196; BstXI--3853; BamHI--3956; SphI--4143; SalI--4232;
CC   AvaI--5006; PvuII--5647; PvuI--7316. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pGW7)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli)(E.coli RR1)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-BamHI 377bp
FT                   \ 4360..4361..376, 3984bp
FT                   2. lambda BamHI-EcoRI 4669bp 34500..39169,
FT                   \ N gene/cI gene/pL/pR
FT                   \ BamHI = 5506 22347 27973 34500 41733
FT                   \ EcoRI = 21227 26105 31748 39169 44973
FT                   -> plasmid 8653bp
FT                   1. plasmid remove BglII-BglII 651bp 38104..38755,
FT                   \ cro [711bp]
FT                   \ BglII = 416 22426 35712 38104 38755 38815
FT                   -> pGW7 8002bp [7944bp]"
FT   -               1..3984
FT                   /note="pBR322 376..4359 3984bp
FT                   EcoRI = G^AATTC"
FT   -               3985..7588
FT                   /note="lambda 34500..38103 3604bp complement
FT                   BglII = A^GATCT"
FT   -               7589..8002
FT                   /note="lambda 38755..39168 414bp complement
FT                   BamHI = G^GATCC"
FT   misc_binding    0..0
FT                   /note="SIT unique EcoRI"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pR"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pM"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="GEN lambda rexA gene"
FT   CDS             0..0
FT                   /note="GEN lambda rexB gene"
FT   promoter        0..0
FT                   /note="PRO bacteriophage lambda pL"
FT   misc_binding    0..0
FT                   /note="SIT unique HpaI"
FT   misc_binding    0..0
FT                   /note="MCS unique BamHI-SphI-SalI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 8002 BP; 2092 A; 1952 C; 1913 G; 2045 T; 0 other;
     gatcctctac gccggacgca tcgtggccgg catcaccggc gccacaggtg cggttgctgg
     cgcctatatc gccgacatca ccgatgggga agatcgggct cgccacttcg ggctcatgag
     cgcttgtttc ggcgtgggta tggtggcagg ccccgtggcc gggggactgt tgggcgccat
     ctccttgcat gcaccattcc ttgcggcggc ggtgctcaac ggcctcaacc tactactggg
     ctgcttccta atgcaggagt cgcataaggg agagcgtcga ccgatgccct tgagagcctt
     caacccagtc agctccttcc ggtgggcgcg gggcatgact atcgtcgccg cacttatgac
     tgtcttcttt atcatgcaac tcgtaggaca ggtgccggca gcgctctggg tcattttcgg
     cgaggaccgc tttcgctgga gcgcgacgat gatcggcctg tcgcttgcgg tattcggaat
     cttgcacgcc ctcgctcaag ccttcgtcac tggtcccgcc accaaacgtt tcggcgagaa
     gcaggccatt atcgccggca tggcggccga cgcgctgggc tacgtcttgc tggcgttcgc
     gacgcgaggc tggatggcct tccccattat gattcttctc gcttccggcg gcatcgggat
     gcccgcgttg caggccatgc tgtccaggca ggtagatgac gaccatcagg gacagcttca
     aggatcgctc gcggctctta ccagcctaac ttcgatcact ggaccgctga tcgtcacggc
     gatttatgcc gcctcggcga gcacatggaa cgggttggca tggattgtag gcgccgccct
     ataccttgtc tgcctccccg cgttgcgtcg cggtgcatgg agccgggcca cctcgacctg
     aatggaagcc ggcggcacct cgctaacgga ttcaccactc caagaattgg agccaatcaa
     ttcttgcgga gaactgtgaa tgcgcaaacc aacccttggc agaacatatc catcgcgtcc
     gccatctcca gcagccgcac gcggcgcatc tcgggcagcg ttgggtcctg gccacgggtg
     cgcatgatcg tgctcctgtc gttgaggacc cggctaggct ggcggggttg ccttactggt
     tagcagaatg aatcaccgat acgcgagcga acgtgaagcg actgctgctg caaaacgtct
     gcgacctgag caacaacatg aatggtcttc ggtttccgtg tttcgtaaag tctggaaacg
     cggaagtcag cgccctgcac cattatgttc cggatctgca tcgcaggatg ctgctggcta
     ccctgtggaa cacctacatc tgtattaacg aagcgctggc attgaccctg agtgattttt
     ctctggtccc gccgcatcca taccgccagt tgtttaccct cacaacgttc cagtaaccgg
     gcatgttcat catcagtaac ccgtatcgtg agcatcctct ctcgtttcat cggtatcatt
     acccccatga acagaaatcc cccttacacg gaggcatcag tgaccaaaca ggaaaaaacc
     gcccttaaca tggcccgctt tatcagaagc cagacattaa cgcttctgga gaaactcaac
     gagctggacg cggatgaaca ggcagacatc tgtgaatcgc ttcacgacca cgctgatgag
     ctttaccgca gctgcctcgc gcgtttcggt gatgacggtg aaaacctctg acacatgcag
     ctcccggaga cggtcacagc ttgtctgtaa gcggatgccg ggagcagaca agcccgtcag
     ggcgcgtcag cgggtgttgg cgggtgtcgg ggcgcagcca tgacccagtc acgtagcgat
     agcggagtgt atactggctt aactatgcgg catcagagca gattgtactg agagtgcacc
     atatgcggtg tgaaataccg cacagatgcg taaggagaaa ataccgcatc aggcgctctt
     ccgcttcctc gctcactgac tcgctgcgct cggtcgttcg gctgcggcga gcggtatcag
     ctcactcaaa ggcggtaata cggttatcca cagaatcagg ggataacgca ggaaagaaca
     tgtgagcaaa aggccagcaa aaggccagga accgtaaaaa ggccgcgttg ctggcgtttt
     tccataggct ccgcccccct gacgagcatc acaaaaatcg acgctcaagt cagaggtggc
     gaaacccgac aggactataa agataccagg cgtttccccc tggaagctcc ctcgtgcgct
     ctcctgttcc gaccctgccg cttaccggat acctgtccgc ctttctccct tcgggaagcg
     tggcgctttc tcatagctca cgctgtaggt atctcagttc ggtgtaggtc gttcgctcca
     agctgggctg tgtgcacgaa ccccccgttc agcccgaccg ctgcgcctta tccggtaact
     atcgtcttga gtccaacccg gtaagacacg acttatcgcc actggcagca gccactggta
     acaggattag cagagcgagg tatgtaggcg gtgctacaga gttcttgaag tggtggccta
     actacggcta cactagaagg acagtatttg gtatctgcgc tctgctgaag ccagttacct
     tcggaaaaag agttggtagc tcttgatccg gcaaacaaac caccgctggt agcggtggtt
     tttttgtttg caagcagcag attacgcgca gaaaaaaagg atctcaagaa gatcctttga
     tcttttctac ggggtctgac gctcagtgga acgaaaactc acgttaaggg attttggtca
     tgagattatc aaaaaggatc ttcacctaga tccttttaaa ttaaaaatga agttttaaat
     caatctaaag tatatatgag taaacttggt ctgacagtta ccaatgctta atcagtgagg
     cacctatctc agcgatctgt ctatttcgtt catccatagt tgcctgactc cccgtcgtgt
     agataactac gatacgggag ggcttaccat ctggccccag tgctgcaatg ataccgcgag
     acccacgctc accggctcca gatttatcag caataaacca gccagccgga agggccgagc
     gcagaagtgg tcctgcaact ttatccgcct ccatccagtc tattaattgt tgccgggaag
     ctagagtaag tagttcgcca gttaatagtt tgcgcaacgt tgttgccatt gctgcaggca
     tcgtggtgtc acgctcgtcg tttggtatgg cttcattcag ctccggttcc caacgatcaa
     ggcgagttac atgatccccc atgttgtgca aaaaagcggt tagctccttc ggtcctccga
     tcgttgtcag aagtaagttg gccgcagtgt tatcactcat ggttatggca gcactgcata
     attctcttac tgtcatgcca tccgtaagat gcttttctgt gactggtgag tactcaacca
     agtcattctg agaatagtgt atgcggcgac cgagttgctc ttgcccggcg tcaacacggg
     ataataccgc gccacatagc agaactttaa aagtgctcat cattggaaaa cgttcttcgg
     ggcgaaaact ctcaaggatc ttaccgctgt tgagatccag ttcgatgtaa cccactcgtg
     cacccaactg atcttcagca tcttttactt tcaccagcgt ttctgggtga gcaaaaacag
     gaaggcaaaa tgccgcaaaa aagggaataa gggcgacacg gaaatgttga atactcatac
     tcttcctttt tcaatattat tgaagcattt atcagggtta ttgtctcatg agcggataca
     tatttgaatg tatttagaaa aataaacaaa taggggttcc gcgcacattt ccccgaaaag
     tgccacctga cgtctaagaa accattatta tcatgacatt aacctataaa aataggcgta
     tcacgaggcc ctttcgtctt caagtttagc tgtcttggtt tgcccaaagc gcattgcata
     atctttcagg gttatgcgtt gttccataca acctccttag tacatgcaac cattatcacc
     gccagaggta aaatagtcaa cacgcacggt gttagatatt tatcccttgc ggtgatagat
     ttaacgtatg agcacaaaaa agaaaccatt aacacaagag cagcttgagg acgcacgtcg
     ccttaaagca atttatgaaa aaaagaaaaa tgaacttggc ttatcccagg aatctgtcgc
     agacaagatg gggatggggc agtcaggcgt tggtgcttta tttaatggca tcaatgcatt
     aaatgcttat aacgccgcat tgcttgcaaa aattctcaaa gttagcgttg aagaatttag
     cccttcaatc gccagagaaa tctacgagat gtatgaagcg gttagtatgc agccgtcact
     tagaagtgag tatgagtacc ctgttttttc tcatgttcag gcagggatgt tctcacctga
     gcttagaacc tttaccaaag gtgatgcgga gagatgggta agcacaacca aaaaagccag
     tgattctgca ttctggcttg aggttgaagg taattccatg accgcaccaa caggctccaa
     gccaagcttt cctgacggaa tgttaattct cgttgaccct gagcaggctg ttgagccagg
     tgatttctgc atagccagac ttgggggtga tgagtttacc ttcaagaaac tgatcaggga
     tagcggtcag gtgtttttac aaccactaaa cccacagtac ccaatgatcc catgcaatga
     gagttgttcc gttgtgggga aagttatcgc tagtcagtgg cctgaagaga cgtttggctg
     atcggcaagg tgttctggtc ggcgcatagc tgataacaat tgagcaagaa tcttcatcga
     attaggggaa ttttcactcc cctcagaaca taacatagta aatggattga attatgaaga
     atggttttta tgcgacttac cgcagcaaaa ataaagggaa agataagcgc tcaataaacc
     tgtctgtttt ccttaattct ctgctggctg ataatcatca cctgcaggtt ggctccaatt
     atttgtatat tcataaaatc gatggaaaaa cttttctctt taccaaaaca aatgacaaga
     gtctggttca gaagataaat cgctctaaag cttcagttga agatattaag aacagcctcg
     cagatgacga atcattggga ttcccatctt ttttgtttgt tgaaggcgac accattggtt
     ttgccagaac tgttttcggg ccgaccacat ccgatctgac agatttttta atcgggaaag
     gaatgtcatt aagcagtgga gagcgcgttc agatagagcc actgatgagg ggaaccacca
     aagacgatgt tatgcatatg catttcatcg gccgaacaac ggtgaaggta gaagccaagc
     tacctgtatt tggcgatata ttaaaggtct taggggcaac agatattgaa ggggagcttt
     ttgactcatt ggatatagtc attaagccaa aatttaaaag ggatataaaa aaggttgcca
     aggatattat ttttaacccg tcacctcaat tttcagacat tagcctgcgg gcaaaagatg
     aggccggaga tattttaaca gaacattatc tatcagaaaa aggccatctc tcagcgcctc
     tgaacaaggt caccaatgct gagatagctg aagagatggc atattgctac gcaagaatga
     aaagtgatat actggaatgt tttaaaaggc aggtgggcaa agttaaggat taattatcag
     gagtaattat gcggaacaga atcatgcctg gtgtttacat agtaataatt ccttacgtta
     tcgtaagcat ttgctatctc cttttccgcc actacattcc tggtgtttct ttttcagctc
     atagagatgg tcttggggcg acattgtcat catatgcagg aaccatgatt gcaatcctga
     ttgctgcctt gacgtttcta atcggaagca gaacgcgccg actggccaag attagagagt
     atgggtatat gacatcggta gttattgtct atgcccttag ttttgttgag cttggagctt
     tgtttttctg cgggttattg cttctttcca gcataagcgg ctacatgata cccactatcg
     ccatcggcat tgcctctgca tcgttcattc atatatgcat ccttgttttc caactatata
     atttgaccag agaacaagaa taacccggcc tcagcgccgg gttttctttg cctcacgatc
     gcccccaaaa cacataacca attgtattta ttgaaaaata aatagataca actcactaaa
     catagcaatt cagatctctc acctaccaaa caatgccccc ctgcaaaaaa taaattcata
     taaaaaacat acagataacc atctgcggtg ataaattatc tctggcggtg ttgacataaa
     taccactggc ggtgatactg agcacatcag caggacgcac tgaccaccat gaaggtgacg
     ctcttaaaaa ttaagccctg aagaagggca gcattcaaag cagaaggctt tggggtgtgt
     gatacgaaac gaagcattgg ccgtaagtgc gattccggat tagctgccaa tgtgccaatc
     gcggggggtt ttcgttcagg actacaactg ccacacacca ccaaagctaa ctgacaggag
     aatccagatg gatgcacaaa cacgccgccg cgaacgtcgc gcagagaaac aggctcaatg
     gaaagcagca aatcccctgt tggttggggt aagcgcaaaa ccagttaacc gccctattct
     ctcgctgaat cgcaaaccga aatcacgagt agaaagcgca ctaaatccga tagaccttac
     agtgctggct gaataccaca aacagattga aagcaacctg caacgtattg agcgcaagaa
     tcagcgcaca tggtacagca agcctggcga acgcggcata acatgcagtg gacgccagaa
     aattaaggga aaatcgattc ctcttatcta gttacttaga tattggcctt ggctttatct
     caatattata tggatcatag ctggcaacta attcagtcca gtaaatatcc tcaataggga
     ataatatatg ctttccattc catcgggaaa aagttttgtt caacacacca agctcaatca
     actcactaat gtatgggaat tgttttgatg taaccacata cttcctgcct tcattaaggg
     ctgcgcacaa aaccatagat tgctcttctg taaggttttg aattactgat cgcactttat
     cgttttgcat cttaatgcgt tttcttagct taaatcgctt atatctggcg ctggcaatag
     ctgataatcg atgcacatta attgctagcg aaaatgcaag agcaaagacg aaaacatgcc
     acacatgagg aataccgatt ctctcattaa catattcagg ccagttatct gggcttaaaa
     gcagaagtcc aacccagata acgatcatat acatggttct ctccagaggt tcattactga
     acactcgtcc gagaataacg agtggatcct ctgacgaata atcttttctt ttttcttttg
     taatagtgtc ttttgtgtcc ccctgttttg agggatagca atcccccaat ttgagggatg
     ttttatccct cgttttaggg gattttccct cgttttgagg gatgcaccat tctgagatgt
     ttttatttgg tccaaacatg ccgccttgct gcttgataat attcattctg acgagttcta
     acttggcttc attgcaccgt ttgacaggta actttgtaat ctcgctaagt tgagaatcgg
     tgattctgtc cattggttta ttccacccat aggttttacg cagaatggca agcagcactt
     taaactgtcg cttggtcaga tctgcgcccg aataagcctc aagcagcata tttgatagtc
     tggcgtaacc atcatcgaga tc
//