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ID   PH2515     preliminary; circular DNA; SYN; 6550 BP.
XX
AC   D90381;
XX
DT   20-JUL-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   Lactobacillus/E. coli plasmid shuttle vector pH2515 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RP   51-1160
RC   pAMbeta1 from Streptococcus adenine methylase gene
RA   Brehm J.K., Salmond G., Minton N.P.;
RT   "Sequence of the adenine methylase gene of the Streptococcus
RT   faecalis plasmid pAMbeta1";
RL   Nucleic Acids Res. 15:3177-3177(1987).
XX
RN   [2]
RP   1-43; 1709-6550
RC   pHY300.1PLK, pHY300.2PLK from pHY300PLK & linker
RC   pHY300.8PLK, pHY300.9PLK from pHY300PLK & lambda
RA   Ishiwa H., Shibahara-Sone H., Shimizu-Kadota M.;
RT   "New shuttle vectors for Escherichia coli and Bacillus subtilis. V.";
RL   Unpublished (1992).
XX
RN   [3]
RP   1-6550
RC   pH2515 from Gram-positive bacteria minus origin & tet gene
RA   Shimizu-Kadota M., Shibahara-Sone H., Ishiwa H.;
RT   "Shuttle plasmid vectors for Lactobacillus casei and Escherichia
RT   coli with a minus origin";
RL   Appl. Environ. Microbiol. 57:3292-3300(1991).
XX
CC   NM (pH2515)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli)(Lactobacillus casei)(Lactobacillus)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (E.faecalis strain DS-5)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pAMbeta1
FT                   2. pHY300PLK 4870bp, MCS/tet gene
FT                   -> pH2515 6550bp"
FT   misc_feature    1..42
FT                   /note="MCS"
FT   -35_signal      223..228
FT                   /note="S35 Gram-positive bacteria MLS resistance gene"
FT   -10_signal      250..255
FT                   /note="S10 Gram-positive bacteria MLS resistance gene"
FT   RBS             298..303
FT                   /note="RBS Gram-positive bacteria MLS resistance gene"
FT   CDS             311..1048
FT                   /note="ANT Gram-positive bacteria MLS resistance gene"
FT   rep_origin      1451..1505
FT                   /note="ORI Gram-positive bacteria minus origin"
FT   CDS             complement(1779..3158)
FT                   /note="ANT Gram-positive bacteria tetracycline
FT                   resistance gene (tet); GTG start codon; partial"
FT   RBS             complement(3163..3167)
FT                   /note="RBS Gram-positive bacteria tetracycline
FT                   resistance gene"
FT   -10_signal      complement(3288..3293)
FT                   /note="S10 Gram-positive bacteria tetracycline
FT                   resistance gene"
FT   -35_signal      complement(3311..3316)
FT                   /note="S35 Gram-positive bacteria tetracycline
FT                   resistance gene"
FT   CDS             complement(3362..4348)
FT                   /note="GEN Gram-positive bacteria Rep-alpha-1 gene
FT                   (replication protein); partial"
FT   RBS             complement(4360..4364)
FT                   /note="RBS Gram-positive bacteria Rep-alpha-1 gene
FT                   (replication protein)"
FT   -10_signal      complement(4448..4454)
FT                   /note="S10 Gram-positive bacteria Rep-alpha-1 gene
FT                   (replication protein)"
FT   -35_signal      complement(4471..4476)
FT                   /note="S35 Gram-positive bacteria Rep-alpha-1 gene
FT                   (replication protein)"
FT   -10_signal      complement(4499..4505)
FT                   /note="S10 Gram-positive bacteria Rep-alpha-1 gene
FT                   (replication protein)"
FT   -35_signal      complement(4520..4527)
FT                   /note="S35 Gram-positive bacteria Rep-alpha-1 gene
FT                   (replication protein)"
FT   rep_origin      complement(4524..4525)
FT                   /note="ORI Gram-positive bacteria origin (nick site)"
FT   -35_signal      4898..4903
FT                   /note="S35 E. coli beta-lactamase gene"
FT   -10_signal      4921..4926
FT                   /note="S10 E. coli beta-lactamase gene"
FT   RBS             4957..4964
FT                   /note="RBS E. coli beta-lactamase gene"
FT   CDS             4968..5828
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_feature    6029..6539
FT                   /note="RNA primer"
XX
SQ   Sequence 6550 BP; 2069 A; 1332 C; 1216 G; 1933 T; 0 other;
     aagcttctag agatctgcag gtcgacggat ccccgggaat tccccgcaca aaaagaaaaa
     cgaaatgata caccaatcag tgcaaaaaaa gatataatgg gagataagac ggttcgtgtt
     cgtgctgact tgcaccatat cataaaaatc gaaacagcaa agaatggcgg aaacgtaaaa
     gaagttatgg aaataagact tagaagcaaa cttaagagtg tgttgatagt gcagtatctt
     aaaattttgt ataataggaa ttgaagttaa attagatgct aaaaatttgt aattaagaag
     gagtgattac atgaacaaaa atataaaata ttctcaaaac tttttaacga gtgaaaaagt
     actcaaccaa ataataaaac aattgaattt aaaagaaacc gataccgttt acgaaattgg
     aacaggtaaa gggcatttaa cgacgaaact ggctaaaata agtaaacagg taacgtctat
     tgaattagac agtcatctat tcaacttatc gtcagaaaaa ttaaaactga atactcgtgt
     cactttaatt caccaagata ttctacagtt tcaattccct aacaaacaga ggtataaaat
     tgttgggagt attccttacc atttaagcac acaaattatt aaaaaagtgg tttttgaaag
     ccatgcgtct gacatctatc tgattgttga agaaggattc tacaagcgta ccttggatat
     tcaccgaaca ctagggttgc tcttgcacac tcaagtctcg attcagcaat tgcttaagct
     gccagcggaa tgctttcatc ctaaaccaaa agtaaacagt gtcttaataa aacttacccg
     ccataccaca gatgttccag ataaatattg gaagctatat acgtactttg tttcaaaatg
     ggtcaatcga gaatatcgtc aactgtttac taaaaatcag tttcatcaag caatgaaaca
     cgccaaagta aacaatttaa gtaccgttac ttatgagcaa gtattgtcta tttttaatag
     ttatctatta tttaacggga ggaaataatt ctatgagtcg cttttgtaaa tttggaaagt
     tacacgttac taaagggaat gtagataaat tattaggtat actactgaca gcttccaagg
     agctaaagag gtccctagcg ggggatctag gcgccttgca ccataaaatt gtaaacgtta
     atattttgtt aaaattcgcg ttaaattttt gttaaatcag ctcatttttt aaccaatagg
     ccgaaatcgg caaaatccct tataaatcaa aagaatagcc cgagataggg ttgagtgttg
     ttccagtttg gaacaagagt ccactattaa agaacgtgga ctccaacgtc aaagggcgaa
     aaaccgtcta tcagggcgat ggcccactac gtgaaccatc acccaaatca agttttttgg
     ggtcgaggtg ccgtaaagca ctaaatcgga accctaaagg gagcccccga tttagagctt
     gacggggaaa gccggcgaac gtggcgagaa aggaagggaa gaaagcgaaa ggagcgggcg
     ctagggcgct ggcaagtgta gcggtcacgc tgcgcgtaac caccacaccc gccgcgctta
     atgcgccgct acagggcgcg tactatggtt gctttgacga gcgcggtgtg aaataccgca
     cagatgcgta aggagaaaat accgcatcag gcgcctaatt cctgttataa aaaaaggatc
     aattttgaac tctctcccaa agttgatccc ttaacgattt agaaatccct ttgagaatgt
     ttatatacat tcaaggtaac cagccaacta atgacaatga ttcctgaaaa aagtaataac
     aaattactat acagataagt tgactgatca acttccatag gtaacaacct ttgatcaagt
     aagggtatgg ataataaacc acctacaatt gcaatacctg ttccctctga taaaaagctg
     gtaaagttaa gcaaactcat tccagcacca gcttcctgct gtttcaagct acttgaaaca
     attgttgata taactgtttt ggtgaacgaa agcccaccta aaacaaatac gattataatt
     gtcatgaacc atgatgttgt ttctaaaaga aaggaagcag ttaaaaagct aacagaaaga
     aatgtaactc cgatgtttaa cacgtataaa ggacctcttc tatcaacaag tatcccacca
     atgtagccga aaataatgac actcattgtt ccagggaaaa taattacact tccgatttcg
     gcagtactta gctggtgaac atctttcatc atataaggaa ccatagagac aaaccctgct
     actgttccaa atataattcc cccacaaaga actccaatca taaaaggtat atttttccct
     aatccgggat caacaaaagg atctgttact ttcctgatat gttttacaaa tatcaggaat
     gacagcacgc taacgataag aaaagaaatg ctatatgatg ttgtaaacaa cataaaaaat
     acaatgccta cagacattag tataattcct ttgatatcaa aatgaccttt tatccttact
     tctttcttta ataatttcat aagaaacgga acagtgataa ttgttatcat aggaatgagt
     agaagatagg accaatgaat ataatgggct atcattccac caatcgctgg accgactcct
     tctcccatgg ctactatcga tccaataaga ccaaatgctt tacccctatt ttcctttgga
     atatagcgcg caactacaac cattacgagt gctggaaatg cagctgcacc agccccttga
     ataaaacgag ccataataag taaggaaaag aaagaatggc caacaaaccc aattaccgac
     ccgaaacaat ttattataat tccaaatagg agtaaccttt tgatgcctaa ttgatcagat
     agctttccat atacagctgt tccaatggaa aaggttaaca taaaggctgt gttcacccag
     tttgtactcg caggtggttt attaaaatca tttgcaatat caggtaatga gacgttcaaa
     accatttcat ttaatacgct aaaaaaagat aaaatgcaaa gccaaattaa aatttggttg
     tgtcgtaaat tcgattgtga ataggatgta ttcacatttc accctccaat aatgagggca
     gacgtagttt atagggttaa tgatacgctt ccctctttta attgaaccct gttacattca
     ttattcatta cacttcataa ttaattcctc ctaaacttga ttaaaacatt ttaccacata
     taaactaagt tttaaattca gtatttcatc acttatacaa caatatggcc cgtttgttga
     actactcttt aataaaataa tttttccgtt cccaattcca cattgcaata atagaaaatc
     catcttcatc ggctttttcg tcatcatctg tatgaatcaa atcgccttct tctgtgtcat
     caaggtttaa ttttttatgt atttctttta acaaaccacc ataggagatt aaccttttac
     ggtgtaaacc ttcctccaaa tcagacaaac gtttcaaatt cttttcttca tcatcggtca
     taaaatccgt atcctttaca ggatattttg cagtttcgtc aattgccgat tgtatatccg
     atttatattt atttttcggt cgaatcattt gaacttttac atttggatca tagtctaatt
     tcattgcctt tttccaaaat tgaatccatt gtttttgatt cacgtagttt tctgtattct
     taaaataagt tggttccaca cataccaata catgcatgtg ctgattataa gaattatctt
     tattatttat tgtcacttcc gttgcacgca taaaaccaac aagattttta ttaatttttt
     tatattgcat cattcggcga aatccttgag ccatatctga caaactctta tttaattctt
     cgccatcata aacattttta actgttaatg tgagaaacaa ccaacgaact gttggctttt
     gtttaataac ttcagcaaca accttttgtg actgaatgcc atgtttcatt gctctcctcc
     agttgcacat tggacaaagc ctggatttac aaaaccacac tcgatacaac tttctttcgc
     ctgtttcacg attttgttta tactctaata tttcagcaca atcttttact ctttcagcct
     ttttaaattc aagaatatgc agaagttcaa agtaatcaac attagcgatt ttcttttctc
     tccatggtct cacttttcca ctttttgtct tgtccactaa aacccttgat ttttcatctg
     aataaatgct actattagga cacataatat taaaagaaac ccccatctat ttagttattt
     gtttggtcac ttataacttt aacagatggg gtttttctgt gcaaccaatt ttaagggttt
     tcaatacttt aaaacacata cataccaaca cttcaacgca cctttcagca actaaaataa
     aaatgacgtt atttctatat gtatcaagat aagaaagaac aagttcaaaa ccatcaaaaa
     aagacacctt ttcaggtgct ttttttattt tataaactca ttccctgatc tcgacttcgt
     tcttttttta cctctcggtt atgagttagt tcaaattcgt tctttttagg ttctaaatcg
     tgtttttctt ggaattgtgc tgttttatcc tttaccttgt ctacaaaccc cttaaaaacg
     tttttaaagg cttttaagcc gtctgtacgt tccttaagga attattcctt agtgctttct
     aggttaatgt catgataata atggtttctt agacgtcagg tggcactttt cggggaaatg
     tgcgcggaac ccctatttgt ttatttttct aaatacattc aaatatgtat ccgctcatga
     gacaataacc ctgataaatg cttcaataat attgaaaaag gaagagtatg agtattcaac
     atttccgtgt cgcccttatt cccttttttg cggcattttg ccttcctgtt tttgctcacc
     cagaaacgct ggtgaaagta aaagatgctg aagatcagtt gggtgcacga gtgggttaca
     tcgaactgga tctcaacagc ggtaagatcc ttgagagttt tcgccccgaa gaacgttttc
     caatgatgag cacttttaaa gttctgctat gtggcgcggt attatcccgt gttgacgccg
     ggcaagagca actcggtcgc cgcatacact attctcagaa tgacttggtt gagtactcac
     cagtcacaga aaagcatctt acggatggca tgacagtaag agaattatgc agtgctgcca
     taaccatgag tgataacact gcggccaact tacttctgac aacgatcgga ggaccgaagg
     agctaaccgc ttttttgcac aacatggggg atcatgtaac tcgccttgat cgttgggaac
     cggagctgaa tgaagccata ccaaacgacg agcgtgacac cacgatgcct gccgcaatgg
     caacaacgtt gcgcaaacta ttaactggcg aactacttac tctagcttcc cggcaacaat
     taatagactg gatggaggcg gataaagttg caggaccact tctgcgctcg gcccttccgg
     ctggctggtt tattgctgat aaatctggag ccggtgagcg tgggtctcgc ggtatcattg
     cagcactggg gccagatggt aagccctccc gtatcgtagt tatctacacg acggggagtc
     aggcaactat ggatgaacga aatagacaga tcgctgagat aggtgcctca ctgattaagc
     attggtaact gtcagaccaa gtttactcat atatacttta gattgattta aaacttcatt
     tttaatttaa aaggatctag gtgaagatcc tttttgataa tctcatgacc aaaatccctt
     aacgtgagtt ttcgttccac tgagcgtcag accccttaat aagatgatct tcttgagatc
     gttttggtct gcgcgtaatc tcttgctctg aaaacgaaaa aaccgccttg cagggaggtt
     tttcgaaggt tctctgagct accaactctt tgaaccgagg taactggctt ggaggagcgc
     agtcaccaaa acttgtcctt tcagtttagc cttaaccggc gcatgacttc aagactaact
     cctctaaatc aattaccagt ggctgctgcc agtggtgctt ttgcatgtct ttccgggttg
     gactcaagac gatagttacc ggataaggcg cagcggtcgg actgaacggg gggttcgtgc
     atacagtcca gcttggagcg aactgcctac ccggaactga gtgtcaggcg tggaatgaga
     caaacgcggc cataacagcg gaatgacacc ggtaaaccga aaggcaggaa caggagagcg
     cacgagggag ccgccagggg gaaacgcctg gtatctttat agtcctgtcg ggtttcgcca
     ccactgattt gagcgtcaga tttcgtgatg cttgtcaggg ggcggagcct atggaaaaac
     ggctttgccc
//