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ID   PHA1       preliminary; circular DNA; SYN; 3888 BP.
XX
AC   IG9818;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pHA1 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJS1, pJS17 from pOP203-3 & lambda, kil gene
RC   pTR12, pTR20 from pJS1
RC   pJS3, pJS10, pJS13, pHA1, pHI74, pHN1 from pBR322 & lambda, kil gene
RC   pHA10 from pHA1 & lambda
RC   p2824 from Charon 3 & pBR322
RC   pKL1 from p2824 & lambda
RA   Honigman A., Oppenheim A.B., Hohn B., Hohn T.;
RT   "Plasmid vectors for positive selection of DNA inserts controlled
RT   by the lambda pL promoter, repressor and antitermination
RT   function";
RL   Gene 13:289-298(1981).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pHA1)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli DR100)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-AvaI 1427bp
FT                   \ 4360..4361..1426, tet gene/2934bp
FT                   2. lambda AvaI-EcoRI 954bp 38215..39169, 79.3% to 81%
FT                   \ lambda EcoRI = 21227 26105 31748 39169 44973
FT                   \ lambda AvaI = 4721 19398 21000 27888 31618 33499
FT                   \ 38215 39889
FT                   -> pHA1 3888bp [no BamHI]"
FT   -               1..2934
FT                   /note="pBR322 1426..4359 2934bp
FT                   EcoRI = G^AATTC"
FT   -               2935..3888
FT                   /note="lambda 38219..39172 954bp complement
FT                   AvaI = C^YCGRG"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3888 BP; 944 A; 980 C; 930 G; 1034 T; 0 other;
     tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattct
     ctgacgaata atcttttctt ttttcttttg taatagtgtc ttttgtgtcc ccctgttttg
     agggatagca atcccccaat ttgagggatg ttttatccct cgttttaggg gattttccct
     cgttttgagg gatgcaccat tctgagatgt ttttatttgg tccaaacatg ccgccttgct
     gcttgataat attcattctg acgagttcta acttggcttc attgcaccgt ttgacaggta
     actttgtaat ctcgctaagt tgagaatcgg tgattctgtc cattggttta ttccacccat
     aggttttacg cagaatggca agcagcactt taaactgtcg cttggtcaga tctgcgcccg
     aataagcctc aagcagcata tttgatagtc tggcgtaacc atcatcgaga tctgccacat
     tacgctcctg tccggcaaag ttacctctgc cgaagttgag tatttttgct gtatttgtca
     taatgactcc tgttgataga tccagtaatg acctcagaac tccatctgga tttgttcaga
     acgctcggtt gccgccgggc gttttttatt ggtgagaatc gcagcaactt gtcgcgccaa
     tcgagccatg tcgtcgtcaa cgacccccca ttcaagaaca gcaagcagca ttgagaactt
     tggaatccag tccctcttcc acctgctgat ctgcgactta tcaacgccca cagcttccgc
     tgtcttctca gttccaagca ttgcgatttt gttaagcaac gcactctcga ttcgtagagc
     ctcgttgcgt ttgtttgcac gaaccatatg taagtatttc cttagataac aattgattga
     atgtatgcaa ataaatgcat acaccatagg tgtggtttaa tttgatgccc tttttcaggg
     ctggaatgtg taagagcggg gttatttatg ctgttgtttt tttgttac
//