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ID   PHA10      preliminary; circular DNA; SYN; 11309 BP.
XX
AC   ATCC37065;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pHA10 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pJS1, pJS17 from pOP203-3 & lambda, kil gene
RC   pTR12, pTR20 from pJS1
RC   pJS3, pJS10, pJS13, pHA1, pHI74, pHN1 from pBR322 & lambda, kil gene
RC   pHA10 from pHA1 & lambda
RC   p2824 from Charon 3 & pBR322
RC   pKL1 from p2824 & lambda
RA   Honigman A., Oppenheim A.B., Hohn B., Hohn T.;
RT   "Plasmid vectors for positive selection of DNA inserts controlled
RT   by the lambda pL promoter, repressor and antitermination
RT   function";
RL   Gene 13:289-298(1981).
XX
RN   [2]
RC   pKB155 from pCR11 & lambda, cI gene
RC   pKB158 from pKB155 & pMB9
RC   pKB252 from pKB158 & lac promoter
RC   [pKB255 from pKB158 & lac promoter]
RA   Backman K., Ptashne M., Gilbert W.;
RT   "Construction of plasmids carrying the cI gene of bacteriophage
RT   lambda";
RL   Proc. Natl. Acad. Sci. U.S.A. 73:4174-4178(1976).
XX
RN   [3]
RC   Charon 1 from lambda & E. coli lac5 & phage phi80
RC   Charon 2 from Charon 1 & phage phi80
RC   Charon 3, Charon 3A from Charon 2 & lambda
RC   Charon 4, Charon 4A from Charon 3 & E. coli bio256
RC   Charon 5 from Charon 1 & E. coli bio1 & lambda
RC   Charon 6 from Charon 5 & phage 434
RC   Charon 7 from Charon 6
RC   Charon 8 from Charon 1 & lambda & phage 434
RC   Charon 9 from lambda & E. coli lac5
RC   Charon 10 from Charon 9 & E. coli bio256
RC   Charon 11 from Charon 10 & phage phi80
RC   Charon 12 from Charon 11 & lambda
RC   Charon 13, Charon 13A from lambda & phage phi80
RC   Charon 14 from Charon 13 & E. coli lac5
RC   Charon 15 from Charon 13 & phage phi80
RC   Charon 16 from Charon 14 & phage phi80
RA   Blattner F., Williams B., Blechl A., Denniston-Thompson K., Faber H.,
RA   Furlong L., Grunwald D., Kiefer D., Moore D., Schumm J., Sheldon E.,
RA   Smithies D.;
RT   "Charon phages, safer derivatives of bacteriophage lambda for DNA
RT   cloning";
RL   Science 196:161-169(1977).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Temperature sensitive at 42C for viability.
CC   A positive selection vector for DNA inserts controlled by lambdaPL
CC   through the regulation of the kil gene.
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pHA10)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli DR100)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(lambda)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 remove EcoRI-AvaI 1427bp
FT                   \ 4360..4361..1426, tet gene/2934bp
FT                   2. lambda AvaI-EcoRI 954bp 38215..39169, 79.3% to 81%
FT                   \ lambda EcoRI = 21227 26105 31748 39169 44973
FT                   \ lambda AvaI = 4721 19398 21000 27888 31618 33499
FT                   \ 38215 39889
FT                   -> pHA1 3888bp [no BamHI]
FT                   1. pHA1 EcoRI 3888bp, pBR322 4360
FT                   2. lambda EcoRI-EcoRI 7421bp 31748..39169,
FT                   \ EcoRI-D/kil/65.4-81%
FT                   -> pHA10 11309bp [cI857 gene]"
FT   -               1..2934
FT                   /note="pBR322 1426..4359 2934bp
FT                   EcoRI = G^AATTC"
FT   -               2935..10355
FT                   /note="lambda 31748..39168 7421bp
FT                   EcoRI = G^AATTC"
FT   -               10356..11309
FT                   /note="lambda 38219..39172 954bp complement
FT                   AvaI = C^YCGRG"
FT   misc_binding    0..0
FT                   /note="SIT unique SalI-BamHI-HpaI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="GEN bacteriophage lambda kil gene;
FT                   reporter gene"
FT   CDS             0..0
FT                   /note="REP bacteriophage lambda cI857 repressor gene"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 11309 BP; 2923 A; 2652 C; 2558 G; 3176 T; 0 other;
     tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggccgagc gcagaagtgg tcctgcaact ttatccgcct
     ccatccagtc tattaattgt tgccgggaag ctagagtaag tagttcgcca gttaatagtt
     tgcgcaacgt tgttgccatt gctgcaggca tcgtggtgtc acgctcgtcg tttggtatgg
     cttcattcag ctccggttcc caacgatcaa ggcgagttac atgatccccc atgttgtgca
     aaaaagcggt tagctccttc ggtcctccga tcgttgtcag aagtaagttg gccgcagtgt
     tatcactcat ggttatggca gcactgcata attctcttac tgtcatgcca tccgtaagat
     gcttttctgt gactggtgag tactcaacca agtcattctg agaatagtgt atgcggcgac
     cgagttgctc ttgcccggcg tcaacacggg ataataccgc gccacatagc agaactttaa
     aagtgctcat cattggaaaa cgttcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattca
     aacagggttc tggcgtcgtt ctcgtactgt tttccccagg ccagtgcttt agcgttaact
     tccggagcca caccggtgca aacctcagca agcagggtgt ggaagtagga cattttcatg
     tcaggccact tctttccgga gcggggtttt gctatcacgt tgtgaacttc tgaagcggtg
     atgacgccga gccgtaattt gtgccacgca tcatccccct gttcgacagc tctcacatcg
     atcccggtac gctgcaggat aatgtccggt gtcatgctgc caccttctgc tctgcggctt
     tctgtttcag gaatccaaga gcttttactg cttcggcctg tgtcagttct gacgatgcac
     gaatgtcgcg gcgaaatatc tgggaacaga gcggcaataa gtcgtcatcc catgttttat
     ccagggcgat cagcagagtg ttaatctcct gcatggtttc atcgttaacc ggagtgatgt
     cgcgttccgg ctgacgttct gcagtgtatg cagtattttc gacaatgcgc tcggcttcat
     ccttgtcata gataccagca aatccgaagg ccagacgggc acactgaatc atggctttat
     gacgtaacat ccgtttggga tgcgactgcc acggccccgt gatttctctg ccttcgcgag
     ttttgaatgg ttcgcggcgg cattcatcca tccattcggt aacgcagatc ggatgattac
     ggtccttgcg gtaaatccgg catgtacagg attcattgtc ctgctcaaag tccatgccat
     caaactgctg gttttcattg atgatgcggg accagccatc aacgcccacc accggaacga
     tgccattctg cttatcagga aaggcgtaaa tttctttcgt ccacggatta aggccgtact
     ggttggcaac gatcagtaat gcgatgaact gcgcatcgct ggcatcacct ttaaatgccg
     tctggcgaag agtggtgatc agttcctgtg ggtcgacaga atccatgccg acacgttcag
     ccagcttccc agccagcgtt gcgagtgcag tactcattcg ttttatacct ctgaatcaat
     atcaacctgg tggtgagcaa tggtttcaac catgtaccgg atgtgttctg ccatgcgctc
     ctgaaactca acatcgtcat caaacgcacg ggtaatggat tttttgctgg ccccgtggcg
     ttgcaaatga tcgatgcata gcgattcaaa caggtgctgg ggcaggcctt tttccatgtc
     gtctgccagt tctgcctctt tctcttcacg ggcgagctgc tggtagtgac gcgcccagct
     ctgagcctca agacgatcct gaatgtaata agcgttcatg gctgaactcc tgaaatagct
     gtgaaaatat cgcccgcgaa atgccgggct gattaggaaa acaggaaagg gggttagtga
     atgcttttgc ttgatctcag tttcagtatt aatatccatt ttttataagc gtcgacggct
     tcacgaaaca tcttttcatc gccaataaaa gtggcgatag tgaatttagt ctggatagcc
     ataagtgttt gatccattct ttgggactcc tggctgatta agtatgtcga taaggcgttt
     ccatccgtca cgtaatttac gggtgattcg ttcaagtaaa gattcggaag ggcagccagc
     aacaggccac cctgcaatgg catattgcat ggtgtgctcc ttatttatac ataacgaaaa
     acgcctcgag tgaagcgtta ttggtatgcg gtaaaaccgc actcaggcgg ccttgatagt
     catatcatct gaatcaaata ttcctgatgt atcgatatcg gtaattctta ttccttcgct
     accatccatt ggaggccatc cttcctgacc atttccatca ttccagtcga actcacacac
     aacaccatat gcatttaagt cgcttgaaat tgctataagc agagcatgtt gcgccagcat
     gattaataca gcatttaata cagagccgtg tttattgagt cggtattcag agtctgacca
     gaaattatta atctggtgaa gtttttcctc tgtcattacg tcatggtcga tttcaatttc
     tattgatgct ttccagtcgt aatcaatgat gtattttttg atgtttgaca tctgttcata
     tcctcacaga taaaaaatcg ccctcacact ggagggcaaa gaagatttcc aataatcaga
     acaagtcggc tcctgtttag ttacgagcga cattgctccg tgtattcact cgttggaatg
     aatacacagt gcagtgttta ttctgttatt tatgccaaaa ataaaggcca ctatcaggca
     gctttgttgt tctgtttacc aagttctctg gcaatcattg ccgtcgttcg tattgcccat
     ttatcgacat atttcccatc ttccattaca ggaaacattt cttcaggctt aaccatgcat
     tccgattgca gcttgcatcc attgcatcgc ttgaattgtc cacaccattg atttttatca
     atagtcgtag tcatacggat agtcctggta ttgttccatc acatcctgag gatgctcttc
     gaactcttca aattcttctt ccatatatca ccttaaatag tggattgcgg tagtaaagat
     tgtgcctgtc ttttaaccac atcaggctcg gtggttctcg tgtaccccta cagcgagaaa
     tcggataaac tattacaacc cctacagttt gatgagtata gaaatggatc cactcgttat
     tctcggacga gtgttcagta atgaacctct ggagagaacc atgtatatga tcgttatctg
     ggttggactt ctgcttttaa gcccagataa ctggcctgaa tatgttaatg agagaatcgg
     tattcctcat gtgtggcatg ttttcgtctt tgctcttgca ttttcgctag caattaatgt
     gcatcgatta tcagctattg ccagcgccag atataagcga tttaagctaa gaaaacgcat
     taagatgcaa aacgataaag tgcgatcagt aattcaaaac cttacagaag agcaatctat
     ggttttgtgc gcagccctta atgaaggcag gaagtatgtg gttacatcaa aacaattccc
     atacattagt gagttgattg agcttggtgt gttgaacaaa actttttccc gatggaatgg
     aaagcatata ttattcccta ttgaggatat ttactggact gaattagttg ccagctatga
     tccatataat attgagataa agccaaggcc aatatctaag taactagata agaggaatcg
     attttccctt aattttctgg cgtccactgc atgttatgcc gcgttcgcca ggcttgctgt
     accatgtgcg ctgattcttg cgctcaatac gttgcaggtt gctttcaatc tgtttgtggt
     attcagccag cactgtaagg tctatcggat ttagtgcgct ttctactcgt gatttcggtt
     tgcgattcag cgagagaata gggcggttaa ctggttttgc gcttacccca accaacaggg
     gatttgctgc tttccattga gcctgtttct ctgcgcgacg ttcgcggcgg cgtgtttgtg
     catccatctg gattctcctg tcagttagct ttggtggtgt gtggcagttg tagtcctgaa
     cgaaaacccc ccgcgattgg cacattggca gctaatccgg aatcgcactt acggccaatg
     cttcgtttcg tatcacacac cccaaagcct tctgctttga atgctgccct tcttcagggc
     ttaattttta agagcgtcac cttcatggtg gtcagtgcgt cctgctgatg tgctcagtat
     caccgccagt ggtatttatg tcaacaccgc cagagataat ttatcaccgc agatggttat
     ctgtatgttt tttatatgaa tttatttttt gcaggggggc attgtttggt aggtgagaga
     tctgaattgc tatgtttagt gagttgtatc tatttatttt tcaataaata caattggtta
     tgtgttttgg gggcgatcgt gaggcaaaga aaacccggcg ctgaggccgg gttattcttg
     ttctctggtc aaattatata gttggaaaac aaggatgcat atatgaatga acgatgcaga
     ggcaatgccg atggcgatag tgggtatcat gtagccgctt atgctggaaa gaagcaataa
     cccgcagaaa aacaaagctc caagctcaac aaaactaagg gcatagacaa taactaccga
     tgtcatatac ccatactctc taatcttggc cagtcggcgc gttctgcttc cgattagaaa
     cgtcaaggca gcaatcagga ttgcaatcat ggttcctgca tatgatgaca atgtcgcccc
     aagaccatct ctatgagctg aaaaagaaac accaggaatg tagtggcgga aaaggagata
     gcaaatgctt acgataacgt aaggaattat tactatgtaa acaccaggca tgattctgtt
     ccgcataatt actcctgata attaatcctt aactttgccc acctgccttt taaaacattc
     cagtatatca cttttcattc ttgcgtagca atatgccatc tcttcagcta tctcagcatt
     ggtgaccttg ttcagaggcg ctgagagatg gcctttttct gatagataat gttctgttaa
     aatatctccg gcctcatctt ttgcccgcag gctaatgtct gaaaattgag gtgacgggtt
     aaaaataata tccttggcaa ccttttttat atccctttta aattttggct taatgactat
     atccaatgag tcaaaaagct ccccttcaat atctgttgcc cctaagacct ttaatatatc
     gccaaataca ggtagcttgg cttctacctt caccgttgtt cggccgatga aatgcatatg
     cataacatcg tctttggtgg ttcccctcat cagtggctct atctgaacgc gctctccact
     gcttaatgac attcctttcc cgattaaaaa atctgtcaga tcggatgtgg tcggcccgaa
     aacagttctg gcaaaaccaa tggtgtcgcc ttcaacaaac aaaaaagatg ggaatcccaa
     tgattcgtca tctgcgaggc tgttcttaat atcttcaact gaagctttag agcgatttat
     cttctgaacc agactcttgt catttgtttt ggtaaagaga aaagtttttc catcgatttt
     atgaatatac aaataattgg agccaacctg caggtgatga ttatcagcca gcagagaatt
     aaggaaaaca gacaggttta ttgagcgctt atctttccct ttatttttgc tgcggtaagt
     cgcataaaaa ccattcttca taattcaatc catttactat gttatgttct gaggggagtg
     aaaattcccc taattcgatg aagattcttg ctcaattgtt atcagctatg cgccgaccag
     aacaccttgc cgatcagcca aacgtctctt caggccactg actagcgata actttcccca
     caacggaaca actctcattg catgggatca ttgggtactg tgggtttagt ggttgtaaaa
     acacctgacc gctatccctg atcagtttct tgaaggtaaa ctcatcaccc ccaagtctgg
     ctatgcagaa atcacctggc tcaacagcct gctcagggtc aacgagaatt aacattccgt
     caggaaagct tggcttggag cctgttggtg cggtcatgga attaccttca acctcaagcc
     agaatgcaga atcactggct tttttggttg tgcttaccca tctctccgca tcacctttgg
     taaaggttct aagctcaggt gagaacatcc ctgcctgaac atgagaaaaa acagggtact
     catactcact tctaagtgac ggctgcatac taaccgcttc atacatctcg tagatttctc
     tggcgattga agggctaaat tcttcaacgc taactttgag aatttttgca agcaatgcgg
     cgttataagc atttaatgca ttgatgccat taaataaagc accaacgcct gactgcccca
     tccccatctt gtctgcgaca gattcctggg ataagccaag ttcatttttc tttttttcat
     aaattgcttt aaggcgacgt gcgtcctcaa gctgctcttg tgttaatggt ttcttttttg
     tgctcatacg ttaaatctat caccgcaagg gataaatatc taacaccgtg cgtgttgact
     attttacctc tggcggtgat aatggttgca tgtactaagg aggttgtatg gaacaacgca
     taaccctgaa agattatgca atgcgctttg ggcaaaccaa gacagctaaa gatctcggcg
     tatatcaaag cgcgatcaac aaggccattc atgcaggccg aaagattttt ttaactataa
     acgctgatgg aagcgtttat gcggaagagg taaagccctt cccgagtaac aaaaaaacaa
     cagcataaat aaccccgctc ttacacattc cagccctgaa aaagggcatc aaattaaacc
     acacctatgg tgtatgcatt tatttgcata cattcaatca attgttatct aaggaaatac
     ttacatatgg ttcgtgcaaa caaacgcaac gaggctctac gaatcgagag tgcgttgctt
     aacaaaatcg caatgcttgg aactgagaag acagcggaag ctgtgggcgt tgataagtcg
     cagatcagca ggtggaagag ggactggatt ccaaagttct caatgctgct tgctgttctt
     gaatgggggg tcgttgacga cgacatggct cgattggcgc gacaagttgc tgcgattctc
     accaataaaa aacgcccggc ggcaaccgag cgttctgaac aaatccagat ggagttctga
     ggtcattact ggatctatca acaggagtca ttatgacaaa tacagcaaaa atactcaact
     tcggcagagg taactttgcc ggacaggagc gtaatgtggc agatctcgat gatggttacg
     ccagactatc aaatatgctg cttgaggctt attcgggcgc agatctgacc aagcgacagt
     ttaaagtgct gcttgccatt ctgcgtaaaa cctatgggtg gaataaacca atggacagaa
     tcaccgattc tcaacttagc gagattacaa agttacctgt caaacggtgc aatgaagcca
     agttagaact cgtcagaatg aatattatca agcagcaagg cggcatgttt ggaccaaata
     aaaacatctc agaatggtgc atccctcaaa acgagggaaa atcccctaaa acgagggata
     aaacatccct caaattgggg gattgctatc cctcaaaaca gggggacaca aaagacacta
     ttacaaaaga aaaaagaaaa gattattcgt cagagaattc tctgacgaat aatcttttct
     tttttctttt gtaatagtgt cttttgtgtc cccctgtttt gagggatagc aatcccccaa
     tttgagggat gttttatccc tcgttttagg ggattttccc tcgttttgag ggatgcacca
     ttctgagatg tttttatttg gtccaaacat gccgccttgc tgcttgataa tattcattct
     gacgagttct aacttggctt cattgcaccg tttgacaggt aactttgtaa tctcgctaag
     ttgagaatcg gtgattctgt ccattggttt attccaccca taggttttac gcagaatggc
     aagcagcact ttaaactgtc gcttggtcag atctgcgccc gaataagcct caagcagcat
     atttgatagt ctggcgtaac catcatcgag atctgccaca ttacgctcct gtccggcaaa
     gttacctctg ccgaagttga gtatttttgc tgtatttgtc ataatgactc ctgttgatag
     atccagtaat gacctcagaa ctccatctgg atttgttcag aacgctcggt tgccgccggg
     cgttttttat tggtgagaat cgcagcaact tgtcgcgcca atcgagccat gtcgtcgtca
     acgacccccc attcaagaac agcaagcagc attgagaact ttggaatcca gtccctcttc
     cacctgctga tctgcgactt atcaacgccc acagcttccg ctgtcttctc agttccaagc
     attgcgattt tgttaagcaa cgcactctcg attcgtagag cctcgttgcg tttgtttgca
     cgaaccatat gtaagtattt ccttagataa caattgattg aatgtatgca aataaatgca
     tacaccatag gtgtggttta atttgatgcc ctttttcagg gctggaatgt gtaagagcgg
     ggttatttat gctgttgttt ttttgttac
//