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ID   PHAGESCRIP preliminary; circular DNA; SYN; 7625 BP.
XX
AC   IG0055;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli phage vector M13 PhageScript - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   PhageScript from pBluescript SK & M13mp18
RA   ;
RT   ;
RL   Unpublished (1992).
XX
CC   The PvuII sites in the LacI portion of m13mp18 were mutagenized
CC   resulting in loss of those sites but not the reading frame.
CC   The PvuII fragment from the Bluescript-SK vectors was the insert for
CC   the M13 vector.
CC   NM (PhageScript)
CC   CM (yes)
CC   NA (filamentous ss-DNA)(ds-DNA)
CC   TP (linear)(circular)
CC   ST ()
CC   TY (phage)
CC   SP (Stratagene)
CC   HO (E.coli JM109)(E.coli JM101)(E.coli NM522)
CC   CP ()
CC   FN (transcription)(sequencing dideoxy)
CC   SE (color blue/white)
CC   PA (pBluescript SK)(M13mp18)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBluescript SK PvuII-PvuII 400bp, lacZ/T3/T7
FT                   2. M13mp18 PvuII 7249bp 5962..5962, lacI
FT                   mutagenesis
FT                   PvuII 7249bp 6055..6055, lacI
FT                   mutagenesis
FT                   PvuII 7249bp 6377..6377, MCS
FT                   -> PhageScript 7625bp"
FT   CDS             496..831
FT                   /note="GEN bacteriophage M13 X gene"
FT   CDS             843..1106
FT                   /note="GEN bacteriophage M13 V gene"
FT   CDS             1107..1208
FT                   /note="GEN bacteriophage M13 VII gene"
FT   CDS             1206..1304
FT                   /note="GEN bacteriophage M13 IX gene"
FT   CDS             1300..1521
FT                   /note="GEN bacteriophage M13 VIII gene"
FT   CDS             1578..2852
FT                   /note="GEN bacteriophage M13 III gene"
FT   CDS             2855..3193
FT                   /note="GEN bacteriophage M13 VI gene"
FT   CDS             3195..4241
FT                   /note="GEN bacteriophage M13 I gene"
FT   CDS             4219..5499
FT                   /note="GEN bacteriophage M13 IV gene"
FT   rep_origin      complement(5488..5868)
FT                   /note="ORI bacteriophage M13 intergenic region
FT                   (M13IG)"
FT   promoter        6095..6216
FT                   /note="PRO E. coli lac gene"
FT   promoter        6471..6490
FT                   /note="PRO bacteriophage T7"
FT   promoter        complement(6616..6631)
FT                   /note="PRO bacteriophage T3"
XX
SQ   Sequence 7625 BP; 1847 A; 1631 C; 1645 G; 2502 T; 0 other;
     aggttattga ccatttgcga aatgtatcta atggtcaaac taaatctact cgttcgcaga
     attgggaatc aactgttaca tggaatgaaa cttccagaca ccgtacttta gttgcatatt
     taaaacatgt tgagctacag caccagattc agcaattaag ctctaagcca tccgcaaaaa
     tgacctctta tcaaaaggag caattaaagg tactctctaa tcctgacctg ttggagtttg
     cttccggtct ggttcgcttt gaagctcgaa ttaaaacgcg atatttgaag tctttcgggc
     ttcctcttaa tctttttgat gcaatccgct ttgcttctga ctataatagt cagggtaaag
     acctgatttt tgatttatgg tcattctcgt tttctgaact gtttaaagca tttgaggggg
     attcaatgaa tatttatgac gattccgcag tattggacgc tatccagtct aaacatttta
     ctattacccc ctctggcaaa acttcttttg caaaagcctc tcgctatttt ggtttttatc
     gtcgtctggt aaacgagggt tatgatagtg ttgctcttac tatgcctcgt aattcctttt
     ggcgttatgt atctgcatta gttgaatgtg gtattcctaa atctcaactg atgaatcttt
     ctacctgtaa taatgttgtt ccgttagttc gttttattaa cgtagatttt tcttcccaac
     gtcctgactg gtataatgag ccagttctta aaatcgcata aggtaattca caatgattaa
     agttgaaatt aaaccatctc aagcccaatt tactactcgt tctggtgttt ctcgtcaggg
     caagccttat tcactgaatg agcagctttg ttacgttgat ttgggtaatg aatatccggt
     tcttgtcaag attactcttg atgaaggtca gccagcctat gcgcctggtc tgtacaccgt
     tcatctgtcc tctttcaaag ttggtcagtt cggttccctt atgattgacc gtctgcgcct
     cgttccggct aagtaacatg gagcaggtcg cggatttcga cacaatttat caggcgatga
     tacaaatctc cgttgtactt tgtttcgcgc ttggtataat cgctgggggt caaagatgag
     tgttttagtg tattctttcg cctctttcgt tttaggttgg tgccttcgta gtggcattac
     gtattttacc cgtttaatgg aaacttcctc atgaaaaagt ctttagtcct caaagcctct
     gtagccgttg ctaccctcgt tccgatgctg tctttcgctg ctgagggtga cgatcccgca
     aaagcggcct ttaactccct gcaagcctca gcgaccgaat atatcggtta tgcgtgggcg
     atggttgttg tcattgtcgg cgcaactatc ggtatcaagc tgtttaagaa attcacctcg
     aaagcaagct gataaaccga tacaattaaa ggctcctttt ggagcctttt tttttggaga
     ttttcaacgt gaaaaaatta ttattcgcaa ttcctttagt tgttcctttc tattctcact
     ccgctgaaac tgttgaaagt tgtttagcaa aaccccatac agaaaattca tttactaacg
     tctggaaaga cgacaaaact ttagatcgtt acgctaacta tgagggttgt ctgtggaatg
     ctacaggcgt tgtagtttgt actggtgacg aaactcagtg ttacggtaca tgggttccta
     ttgggcttgc tatccctgaa aatgagggtg gtggctctga gggtggcggt tctgagggtg
     gcggttctga gggtggcggt actaaacctc ctgagtacgg tgatacacct attccgggct
     atacttatat caaccctctc gacggcactt atccgcctgg tactgagcaa aaccccgcta
     atcctaatcc ttctcttgag gagtctcagc ctcttaatac tttcatgttt cagaataata
     ggttccgaaa taggcagggg gcattaactg tttatacggg cactgttact caaggcactg
     accccgttaa aacttattac cagtacactc ctgtatcatc aaaagccatg tatgacgctt
     actggaacgg taaattcaga gactgcgctt tccattctgg ctttaatgaa gatccattcg
     tttgtgaata tcaaggccaa tcgtctgacc tgcctcaacc tcctgtcaat gctggcggcg
     gctctggtgg tggttctggt ggcggctctg agggtggtgg ctctgagggt ggcggttctg
     agggtggcgg ctctgaggga ggcggttccg gtggtggctc tggttccggt gattttgatt
     atgaaaagat ggcaaacgct aataaggggg ctatgaccga aaatgccgat gaaaacgcgc
     tacagtctga cgctaaaggc aaacttgatt ctgtcgctac tgattacggt gctgctatcg
     atggtttcat tggtgacgtt tccggccttg ctaatggtaa tggtgctact ggtgattttg
     ctggctctaa ttcccaaatg gctcaagtcg gtgacggtga taattcacct ttaatgaata
     atttccgtca atatttacct tccctccctc aatcggttga atgtcgccct tttgtcttta
     gcgctggtaa accatatgaa ttttctattg attgtgacaa aataaactta ttccgtggtg
     tctttgcgtt tcttttatat gttgccacct ttatgtatgt attttctacg tttgctaaca
     tactgcgtaa taaggagtct taatcatgcc agttcttttg ggtattccgt tattattgcg
     tttcctcggt ttccttctgg taactttgtt cggctatctg cttacttttc ttaaaaaggg
     cttcggtaag atagctattg ctatttcatt gtttcttgct cttattattg ggcttaactc
     aattcttgtg ggttatctct ctgatattag cgctcaatta ccctctgact ttgttcaggg
     tgttcagtta attctcccgt ctaatgcgct tccctgtttt tatgttattc tctctgtaaa
     ggctgctatt ttcatttttg acgttaaaca aaaaatcgtt tcttatttgg attgggataa
     ataatatggc tgtttatttt gtaactggca aattaggctc tggaaagacg ctcgttagcg
     ttggtaagat tcaggataaa attgtagctg ggtgcaaaat agcaactaat cttgatttaa
     ggcttcaaaa cctcccgcaa gtcgggaggt tcgctaaaac gcctcgcgtt cttagaatac
     cggataagcc ttctatatct gatttgcttg ctattgggcg cggtaatgat tcctacgatg
     aaaataaaaa cggcttgctt gttctcgatg agtgcggtac ttggtttaat acccgttctt
     ggaatgataa ggaaagacag ccgattattg attggtttct acatgctcgt aaattaggat
     gggatattat ttttcttgtt caggacttat ctattgttga taaacaggcg cgttctgcat
     tagctgaaca tgttgtttat tgtcgtcgtc tggacagaat tactttacct tttgtcggta
     ctttatattc tcttattact ggctcgaaaa tgcctctgcc taaattacat gttggcgttg
     ttaaatatgg cgattctcaa ttaagcccta ctgttgagcg ttggctttat actggtaaga
     atttgtataa cgcatatgat actaaacagg ctttttctag taattatgat tccggtgttt
     attcttattt aacgccttat ttatcacacg gtcggtattt caaaccatta aatttaggtc
     agaagatgaa attaactaaa atatatttga aaaagttttc tcgcgttctt tgtcttgcga
     ttggatttgc atcagcattt acatatagtt atataaccca acctaagccg gaggttaaaa
     aggtagtctc tcagacctat gattttgata aattcactat tgactcttct cagcgtctta
     atctaagcta tcgctatgtt ttcaaggatt ctaagggaaa attaattaat agcgacgatt
     tacagaagca aggttattca ctcacatata ttgatttatg tactgtttcc attaaaaaag
     gtaattcaaa tgaaattgtt aaatgtaatt aattttgttt tcttgatgtt tgtttcatca
     tcttcttttg ctcaggtaat tgaaatgaat aattcgcctc tgcgcgattt tgtaacttgg
     tattcaaagc aatcaggcga atccgttatt gtttctcccg atgtaaaagg tactgttact
     gtatattcat ctgacgttaa acctgaaaat ctacgcaatt tctttatttc tgttttacgt
     gctaataatt ttgatatggt tggttcaatt ccttccataa ttcagaagta taatccaaac
     aatcaggatt atattgatga attgccatca tctgataatc aggaatatga tgataattcc
     gctccttctg gtggtttctt tgttccgcaa aatgataatg ttactcaaac ttttaaaatt
     aataacgttc gggcaaagga tttaatacga gttgtcgaat tgtttgtaaa gtctaatact
     tctaaatcct caaatgtatt atctattgac ggctctaatc tattagttgt tagtgcacct
     aaagatattt tagataacct tcctcaattc ctttctactg ttgatttgcc aactgaccag
     atattgattg agggtttgat atttgaggtt cagcaaggtg atgctttaga tttttcattt
     gctgctggct ctcagcgtgg cactgttgca ggcggtgtta atactgaccg cctcacctct
     gttttatctt ctgctggtgg ttcgttcggt atttttaatg gcgatgtttt agggctatca
     gttcgcgcat taaagactaa tagccattca aaaatattgt ctgtgccacg tattcttacg
     ctttcaggtc agaagggttc tatctctgtt ggccagaatg tcccttttat tactggtcgt
     gtgactggtg aatctgccaa tgtaaataat ccatttcaga cgattgagcg tcaaaatgta
     ggtatttcca tgagcgtttt tcctgttgca atggctggcg gtaatattgt tctggatatt
     accagcaagg ccgatagttt gagttcttct actcaggcaa gtgatgttat tactaatcaa
     agaagtattg ctacaacggt taatttgcgt gatggacaga ctcttttact cggtggcctc
     actgattata aaaacacttc tcaagattct ggcgtaccgt tcctgtctaa aatcccttta
     atcggcctcc tgtttagctc ccgctctgat tccaacgagg aaagcacgtt atacgtgctc
     gtcaaagcaa ccatagtacg cgccctgtag cggcgcatta agcgcggcgg gtgtggtggt
     tacgcgcagc gtgaccgcta cacttgccag cgccctagcg cccgctcctt tcgctttctt
     cccttccttt ctcgccacgt tcgccggctt tccccgtcaa gctctaaatc gggggctccc
     tttagggttc cgatttagtg ctttacggca cctcgacccc aaaaaacttg atttgggtga
     tggttcacgt agtgggccat cgccctgata gacggttttt cgccctttga cgttggagtc
     cacgttcttt aatagtggac tcttgttcca aactggaaca acactcaacc ctatctcggg
     ctattctttt gatttataag ggattttgcc gatttcggaa ccaccatcaa acaggatttt
     cgcctgctgg ggcaaaccag cgtggaccgc ttgctgcaac tctctcaggg ccaggcggtg
     aagggcaatc aactgttgcc cgtctcactg gtgaaaagaa aaaccaccct ggcgcccaat
     acgcaaaccg cctctccccg cgcgttggcc gattcattaa tgcaactggc acgacaggtt
     tcccgactgg aaagcgggca gtgagcgcaa cgcaattaat gtgagttacc tcactcatta
     ggcaccccag gctttacact ttatgcttcc ggctcgtatg ttgtgtggaa ttgtgagcgg
     ataacaattt cacacaggaa acagctatga ccatgattac gaattcgagc tcggtacccg
     gggatcctct agagtcgacc tgcaggcatg caagcttggc actggccgtc gttttacaac
     gtcgtgactg ggaaaaccct ggcgttaccc aacttaatcg ccttgcagca catccccctt
     tcgccagctg gcgaaggggg gatgtgctgc aaggcgatta agttgggtaa cgccagggtt
     ttcccagtca cgacgttgta aaacgacggc cagtgaattg taatacgact cactataggg
     cgaattgggt accgggcccc ccctcgaggt cgacggtatc gataagcttg atatcgaatt
     cctgcagccc gggggatcca ctagttctag agcggccgcc accgcggtgg agctccagct
     tttgttccct ttagtgaggg ttaattccga gcttggcgta atcatggtca tagctgtttc
     ctgtgtgaaa ttgttatccg ctcacaattc cacacaacat acgagccgga agcataaagt
     gtaaagcctg gggtgcctaa tgagtgaggt aactcacatt aattgcgttg cgctcactgc
     ccgctttcca gtcgggaaac ctgtcgtgcc agctggcgta atagcgaaga ggcccgcacc
     gatcgccctt cccaacagtt gcgtagcctg aatggcgaat ggcgctttgc ctggtttccg
     gcaccagaag cggtgccgga aagctggctg gagtgcgatc ttcctgaggc cgatactgtc
     gtcgtcccct caaactggca gatgcacggt tacgatgcgc ccatctacac caacgtaacc
     tatcccatta cggtcaatcc gccgtttgtt cccacggaga atccgacggg ttgttactcg
     ctcacattta atgttgatga aagctggcta caggaaggcc agacgcgaat tatttttgat
     ggcgttccta ttggttaaaa aatgagctga tttaacaaaa atttaacgcg aattttaaca
     aaatattaac gtttacaatt taaatatttg cttatacaat cttcctgttt ttggggcttt
     tctgattatc aaccggggta catatgattg acatgctagt tttacgatta ccgttcatcg
     attctcttgt ttgctccaga ctctcaggca atgacctgat agcctttgta gatctctcaa
     aaatagctac cctctccggc atgaatttat cagctagaac ggttgaatat catattgatg
     gtgatttgac tgtctccggc ctttctcacc cttttgaatc tttacctaca cattactcag
     gcattgcatt taaaatatat gagggttcta aaaattttta tccttgcgtt gaaataaagg
     cttctcccgc aaaagtatta cagggtcata atgtttttgg tacaaccgat ttagctttat
     gctctgaggc tttattgctt aattttgcta attctttgcc ttgcctgtat gatttattgg
     atgtt
//