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ID   PHC312     preliminary; circular DNA; SYN; 2014 BP.
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AC   IG9820;
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DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli K12 plasmid vector pHC312 - complete, lacUV5 promoter & MCS.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHC1 from pBR322
RC   pHC81 from pHC1
RC   pHC314 from pHC81 & piVX
RC   pHC312 from pHC314
RC   pHC624 from pHC312 & piAN7
RA   Boros I., Posfai G., Venetianer P.;
RT   "High-copy-number derivatives of the plasmid cloning vector pBR322";
RL   Gene 30:257-260(1984).
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CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pHC312)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli K-12)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(piVX)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pBR322 BamHI-EcoRI 3984bp 376..4360
FT                   2. insert 1600bp
FT                   -> pHC1 6000bp
FT                   1. pHC1 large PvuII-EcoRI 2293bp, no oligo insert
FT                   \ pBR322 2293bp 2067..4360
FT                   :Klenow
FT                   -> pHC81 2295bp [unique EcoRI]
FT                   1. pHC81 EcoRI 2295bp 0..0, insert
FT                   2. piVX EcoRI-EcoRI 109bp 2..111, MCS
FT                   \ EcoRI = 2 111 314
FT                   -> pHC314 2405bp
FT                   1. pHC314 HinfI-BamHI 2006bp, 2449..4360/2..97
FT                   \ piVX BamHI = 97
FT                   \ piVX HinfI = 152 187 645
FT                   \ pBR322 HinfI =  633 853 1007 1305 1526 2030 2374
FT                   \ 2449 2845 3362
FT                   Klenow:Klenow
FT                   -> pHC312 2010bp"
FT                   \ [unique EcoRI, ClaI, HindIII, XbaI, BglII]
FT   -               1..1915
FT                   /note="pBR322 2449..4361..2 1915bp
FT                   EcoRI = G^AATT C
FT                   PvuII =    CAG^CTG
FT                   EcoRI = G^AATT C"
FT   -               1916..2014
FT                   /note="piVX 2..100 99bp
FT                   BamHI = G^GATC C
FT                   HinfI =      G^ANTC"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
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SQ   Sequence 2014 BP; 526 A; 505 C; 465 G; 518 T; 0 other;
     aatcagggga taacgcagga aagaacatgt gagcaaaagg ccagcaaaag gccaggaacc
     gtaaaaaggc cgcgttgctg gcgtttttcc ataggctccg cccccctgac gagcatcaca
     aaaatcgacg ctcaagtcag aggtggcgaa acccgacagg actataaaga taccaggcgt
     ttccccctgg aagctccctc gtgcgctctc ctgttccgac cctgccgctt accggatacc
     tgtccgcctt tctcccttcg ggaagcgtgg cgctttctca tagctcacgc tgtaggtatc
     tcagttcggt gtaggtcgtt cgctccaagc tgggctgtgt gcacgaaccc cccgttcagc
     ccgaccgctg cgccttatcc ggtaactatc gtcttgagtc caacccggta agacacgact
     tatcgccact ggcagcagcc actggtaaca ggattagcag agcgaggtat gtaggcggtg
     ctacagagtt cttgaagtgg tggcctaact acggctacac tagaaggaca gtatttggta
     tctgcgctct gctgaagcca gttaccttcg gaaaaagagt tggtagctct tgatccggca
     aacaaaccac cgctggtagc ggtggttttt ttgtttgcaa gcagcagatt acgcgcagaa
     aaaaaggatc tcaagaagat cctttgatct tttctacggg gtctgacgct cagtggaacg
     aaaactcacg ttaagggatt ttggtcatga gattatcaaa aaggatcttc acctagatcc
     ttttaaatta aaaatgaagt tttaaatcaa tctaaagtat atatgagtaa acttggtctg
     acagttacca atgcttaatc agtgaggcac ctatctcagc gatctgtcta tttcgttcat
     ccatagttgc ctgactcccc gtcgtgtaga taactacgat acgggagggc ttaccatctg
     gccccagtgc tgcaatgata ccgcgagacc cacgctcacc ggctccagat ttatcagcaa
     taaaccagcc agccggaagg gccgagcgca gaagtggtcc tgcaacttta tccgcctcca
     tccagtctat taattgttgc cgggaagcta gagtaagtag ttcgccagtt aatagtttgc
     gcaacgttgt tgccattgct gcaggcatcg tggtgtcacg ctcgtcgttt ggtatggctt
     cattcagctc cggttcccaa cgatcaaggc gagttacatg atcccccatg ttgtgcaaaa
     aagcggttag ctccttcggt cctccgatcg ttgtcagaag taagttggcc gcagtgttat
     cactcatggt tatggcagca ctgcataatt ctcttactgt catgccatcc gtaagatgct
     tttctgtgac tggtgagtac tcaaccaagt cattctgaga atagtgtatg cggcgaccga
     gttgctcttg cccggcgtca acacgggata ataccgcgcc acatagcaga actttaaaag
     tgctcatcat tggaaaacgt tcttcggggc gaaaactctc aaggatctta ccgctgttga
     gatccagttc gatgtaaccc actcgtgcac ccaactgatc ttcagcatct tttactttca
     ccagcgtttc tgggtgagca aaaacaggaa ggcaaaatgc cgcaaaaaag ggaataaggg
     cgacacggaa atgttgaata ctcatactct tcctttttca atattattga agcatttatc
     agggttattg tctcatgagc ggatacatat ttgaatgtat ttagaaaaat aaacaaatag
     gggttccgcg cacatttccc cgaaaagtgc cacctgacgt ctaagaaacc attattatca
     tgacattaac ctataaaaat aggcgtatca cgaggccctt tcgtcttcaa gaattaattc
     tcatgtttga cagcttatca tcgataagct tctagagatc ttccatacct accagttctc
     cgcctgcagc aatggcaaca acgttgcccg gatc
//