Return to this vector's summary.
ID PHC79 preliminary; circular DNA; SYN; 6524 BP.
XX
AC L08873; VB0100; IG0165; ATCC37030;
XX
DT 02-NOV-1992 (Rel. 7, Created)
DT 01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE E. coli cosmid vector pHC79 - complete.
XX
KW cloning vector.
XX
OS Cloning vector
OC Artificial sequences; Cloning vehicles.
XX
RN [1]
RC [plambda2H1 from lambda]
RC pBHB2830 from Charon 4A & plambda2H1
RC pJC80 from pBR322 & Charon 4A
RC pHC79 from pJC80 & pBHB2830
RA Hohn B., Collins J.;
RT "A small cosmid for efficient cloning of large DNA fragments";
RL Gene 11:291-298(1980).
XX
RN [2]
RC pHC79
RA Collins J.;
RT ;
RL Submitted (11-NOV-1986) over computer network by:
RL Collins J., .
XX
RN [3]
RC lambda Dam srIlambda3 from lambda
RC lambda P1:3 from lambda
RA Enquist L.W., Sternberg N.;
RT "In vitro packaging of lambda Dam vectors and their use in cloning
RT DNA fragments";
RL Meth. Enzymol. 68:281-298(1979).
XX
RN [4]
RC [lambda b2 from lambda]
RC from pJC75-58
RC from Charon 4A
RC from lambda b2
RA Hohn B.;
RT "In vitro packaging of lambda and cosmid DNA";
RL Meth. Enzymol. 68:299-309(1979).
XX
RN [5]
RP 1-6524
RC pHC79
RA Gilbert W.;
RT "VecBase 3.0";
RL Unpublished (1991).
XX
RN [6]
RC pJC81 from lambda, cos
RA Collins J.;
RT ;
RL Unpublished (1983).
XX
RN [7]
RC pJC81 from tet gene & carb gene & lambda, cos
RA Holsters M., Villaroel R., Van Montagu M., Schell J.;
RT "The use of selectable markers for the isolation of plant-DNA/T-DNA
RT junction fragments in a cosmid vector";
RL Mol. Gen. Genet. 185:283-289(1982).
XX
RN [8]
RC pJC76 from pJC720 & pJC75-58
RC pJC77 from pBR322 & pJC75-58
RC pJC78 from pBR322 & pJC75-58
RC pJC80 from pBR322
RC pJC79 or pHC79 from pJC80 & Charon 3A
RC pJB4 from pJC75-58
RA Collins J.;
RT "Escherichia coli plasmids packageable in vitro in bacteriophage
RT particles";
RL Meth. Enzymol. 68:309-326(1979).
XX
RN [9]
RC pJC703 from pBR322
RC pJC720 from pBR322
RA Collins J.;
RT "Gene cloning with small plasmids";
RL Curr. Top. Microbiol. Immunol. 78:122-170(1977).
XX
RN [10]
RC pJC74 from pJC703 & R1drd-19
RC pJC75-49, pJC75-69, pJC75-40, pJC75-35, pJC75-65 from pJC74
RC pJC75-58, pJC75-37 from pJC74
RC pJC703delta from pJC703
RC pJC720delta from pJC720
RA Collins J., Bruning H.J.;
RT "Plasmids useable as gene-cloning vectors in an in vitro packaging
RT by coliphage lambda: cosmids";
RL Gene 4:85-107(1978).
XX
CC Boehringer size is 6524 bp.
CC BRL size is 6523 bp; it is missing the c at 6481.
CC ATCC size is 6430 bp.
CC The lambda cohesive end site (cos region) is needed for packaging
CC into pre-mature phage heads.
CC pBR322 was partially digested with Sau3A and ligated with an 0.65
CC BglII lambda fragment, generating a BglII site (pBR322 positions
CC 1459-1666 deleted). A 1.65 kb BglII fragment from Charon 4A was
CC ligated into the BglII site. [1]
CC A general purpose cosmid vector derived from pBR322. [1]
CC Medium is 1227 LB plus ampicillin.
CC old Boehringer vector.
CC NM (pHC79)
CC CM (yes)
CC NA (ds-DNA)
CC TP (circular)
CC ST ()
CC TY (cosmid)
CC SP (BRL)(ATCC)
CC HO (E.coli HB101)(E.coli LE392)(E.coli JM83)(E.coli 5K)(E.coli)
CC CP ()
CC FN (cloning 30000 to 40000 bp)(in vitro packaging)
CC SE ()
CC PA (pBR322)(lambda cos)
CC BR ()
CC OF (pV34)
CC OR ()
XX
FH Key Location/Qualifiers
FH
FT misc_feature 0..0
FT /note="1. lambda
FT -> plambda2H1
FT 1. Charon 4A BglII-BglII 1650bp, lambda cos
FT 2. plambda2H1 BglII
FT -> pBHB2830
FT 1. pBR322 remove Sau3A/MboI-Sau3A/MboI 137bp
FT \ 4224bp
FT 2. Charon 4A BglII-BglII 650bp, lambda cro/cII
FT -> pJC80 4874bp
FT 1. pJC80 BglII 4874bp
FT 2. pBHB2830 BglII-BglII 1650bp, lambda cos
FT -> pHC79 6524bp [unique EcaI at lambda cos]"
FT misc_feature 1..1464
FT /note="from pBR322 bases 1..1464"
FT misc_binding 23..23
FT /note="SIT unique ClaI"
FT misc_binding 29..29
FT /note="SIT unique HindIII"
FT CDS 86..1276
FT /note="ANT E. coli tetracycline resistance gene (tet)"
FT misc_binding 375..375
FT /note="SIT unique BamHI"
FT misc_binding 651..651
FT /note="SIT unique SalI"
FT misc_feature 1461..2115
FT /note="from bacteriophage lambda bases
FT complement(38103..38758)"
FT misc_feature 2115..3702
FT /note="from bacteriophage lambda bases
FT complement(1..1588)"
FT misc_binding >3240..<3500
FT /note="SIT lambda cohesive end site (cos)"
FT misc_feature 3703..3839
FT /note="from bacteriophage lambda bases
FT complement(48366..48502)"
FT misc_feature 3830..6524
FT /note="from pBR322 bases 1668..4363"
FT rep_origin complement(4700..4700)
FT /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT CDS complement(5458..6321)
FT /note="ANT E. coli beta-lactamase gene (bla)
FT ampicillin resistance gene (apr/amp)"
FT misc_binding 5774..5774
FT /note="SIT unique PstI"
FT misc_binding 6522..6522
FT /note="SIT unique EcoRI"
FT conflict 1761..1761
FT /note="G in pHC79; C in bacteriophage lambda bases
FT complement(38458..38458)"
FT conflict 1972..1973
FT /note="GT in pHC79; GGT in bacteriophage lambda
FT bases complement(38245..38247)"
FT conflict 2095..2095
FT /note="A in pHC79; C in bacteriophage lambda bases
FT complement(38123..38123)"
FT conflict 2159..2159
FT /note="T in pHC79; G in bacteriophage lambda bases
FT complement(1544..1544)"
FT conflict 2568..2568
FT /note="A in pHC79; T in bacteriophage lambda bases
FT complement(1135..1135)"
FT conflict 2970..2970
FT /note="T in pHC79; A in bacteriophage lambda bases
FT complement(733..733)"
FT conflict 3184..3184
FT /note="A in pHC79; T in bacteriophage lambda bases
FT complement(519..519)"
FT conflict 3283..3283
FT /note="G in pHC79; A in bacteriophage lambda bases
FT complement(420..420)"
FT conflict 3829..3829
FT /note="A in pHC79; T in bacteriophage lambda bases
FT complement(48376..48376)"
FT conflict 4547..4547
FT /note="T in pHC79; G in pBR322 bases 2385..2385"
FT conflict 5344..5345
FT /note="TA in pHC79; TAA in pBR322 bases 3182..3184"
XX
SQ Sequence 6524 BP; 1514 A; 1792 C; 1618 G; 1600 T; 0 other;
ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
tcctggccac gggtgcgcat gatctgccac attacgctcc tgtccggcaa agttacctct
gccgaagttg agtatttttg ctgtatttgt cataatgact cctgttgata gatccagtaa
tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
ttggtgagaa tcgcagcaac ttgtcgcgcc aatcgagcca tgtcgtcgtc aacgaccccc
cattcaagaa cagcaagcag cattgagaac tttggaatcc agtccctctt ccacctgctg
atctgcgact tatcaacgcc gacagcttcc gctgtcttct cagttccaag cattgcgatt
ttgttaagca acgcactctc gattcgtaga gcctcgttgc gtttgtttgc acgaaccata
tgtaagtatt tccttagata acaattgatt gaatgtatgc aaataaatgc atacaccata
ggtgtggttt aatttgatgc cctttttcag ggctggaatg tgtaagagcg ggttatttat
gctgttgttt ttttgttact cgggaagggc tttacctctt ccgcataaac gcttccatca
gcgtttatag ttaaaaaaat ctttcggcct gcatgaatgg ccttgttgat cgcgatttga
tatacgccga gatctaacgg gcatcagtaa agtccagctc ctgctggcgg atgacgcatg
cattatgctc gcagagataa aacacgctgg aggggtcatc cggcgtccat ttgaggccaa
acggcgtctc tttgtcgcca aatttaagat actgctcctc cccgcaatgc gggcaggcaa
catgaaaacg cataaaatgc ggggattcac tggctgcacg ctcaatctga caggtgcctc
tcacttttgg cgtggagcca cggatggact ttggccagac cgagccttca atacgcttgt
cacccaggaa cgtcggagag ccttcctgtt caatatcatc atcaaaagca gcaagttcat
cataacccgc cacatccacc gacttttcac ggtagttttt tgccgcttta ccgcccaggc
accagaagcc acgcccatta gtgaaacgct tcatggtgag cgtgttaacc cggtgctttt
tgccatacca cggggccagc gccagcagcg acggaatatc acgaatagtc ggctcaacgt
gggttttcat aaagttctcg gcatcaccat ccgtcggcaa ccagataagg gtgttgcgct
gcttatgctc tataaagtag gcataaacac ccagcagcat tttggaataa ccgacacggg
cagacttcac cacattcacc tcacggatgt agtcgctgcc catcgcattc atgatggccc
gctgaaaggg cagtgtttcc cagcgccctt cctggtatgc ggattctttc gggagatagt
aattagcatc cgcccattca acggcggtct gtggctccgg cctgaacagt gagcgaagcc
cggcgcggac aaaatgccgc agcctgttat cctgactgtt cgatatattc actcagcaac
cccggtatca gttcatccag cgcggctgct ttgttcatgg ctttgatgat atcccgtttc
aggaaatcaa catgtcggtt ttccagttcc ggaaaacgcc gctgcaccga cagggggagc
ccgtcgagaa tactggcaat ttcacctgcg atccgcgaca gcacgaaagt acagaatgcg
gttaccacca cttcagcgga gtctctggca ttcttcagtt cctgtgcgtc ggcctgcgca
cgcgtaagtc gatggcgttc gtactcaata gttcctggct ggggatctgc ctcgctggcc
tgccgcagtt cttcaacctc ccggcgcagc ttttcgttct caatttcagc atccctttcg
gcataccatt ttatgacggc ggcagagtca taaagcacct cattaccctt gccaccgcct
cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact cgcaccgaaa
atgtcagcca gctgcttttt gttgacttcc attgttcatt ccacggacaa aaacagagaa
aggaaacgac agaggccaaa aagcctcgct ttcagcacct gtcgtttcct ttcttttcag
agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg ccttaaaccg
gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc cccgtaacct gtcggatcac
cggaaaggac ccgtaaagtg ataatgatta tcatctacat atcacaacgt gcgtggaggc
catcaaacca cgtcaaataa tcaattatga cgcaggtatc gtattaatag atctgcatcg
caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt
gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac
aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc
gtttcatcgg tatcattacc cccatgaaca gaaattcccc cttacacgga ggcatcaagt
gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgctctc ggtcgttcgg
ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
cgttagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//