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ID   PHC79      preliminary; circular DNA; SYN; 6524 BP.
XX
AC   L08873; VB0100; IG0165; ATCC37030;
XX
DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli cosmid vector pHC79 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   [plambda2H1 from lambda]
RC   pBHB2830 from Charon 4A & plambda2H1
RC   pJC80 from pBR322 & Charon 4A
RC   pHC79 from pJC80 & pBHB2830
RA   Hohn B., Collins J.;
RT   "A small cosmid for efficient cloning of large DNA fragments";
RL   Gene 11:291-298(1980).
XX
RN   [2]
RC   pHC79
RA   Collins J.;
RT   ;
RL   Submitted (11-NOV-1986) over computer network by:
RL   Collins J., .
XX
RN   [3]
RC   lambda Dam srIlambda3 from lambda
RC   lambda P1:3 from lambda
RA   Enquist L.W., Sternberg N.;
RT   "In vitro packaging of lambda Dam vectors and their use in cloning
RT   DNA fragments";
RL   Meth. Enzymol. 68:281-298(1979).
XX
RN   [4]
RC   [lambda b2 from lambda]
RC   from pJC75-58
RC   from Charon 4A
RC   from lambda b2
RA   Hohn B.;
RT   "In vitro packaging of lambda and cosmid DNA";
RL   Meth. Enzymol. 68:299-309(1979).
XX
RN   [5]
RP   1-6524
RC   pHC79
RA   Gilbert W.;
RT   "VecBase 3.0";
RL   Unpublished (1991).
XX
RN   [6]
RC   pJC81 from lambda, cos
RA   Collins J.;
RT   ;
RL   Unpublished (1983).
XX
RN   [7]
RC   pJC81 from tet gene & carb gene & lambda, cos
RA   Holsters M., Villaroel R., Van Montagu M., Schell J.;
RT   "The use of selectable markers for the isolation of plant-DNA/T-DNA
RT   junction fragments in a cosmid vector";
RL   Mol. Gen. Genet. 185:283-289(1982).
XX
RN   [8]
RC   pJC76 from pJC720 & pJC75-58
RC   pJC77 from pBR322 & pJC75-58
RC   pJC78 from pBR322 & pJC75-58
RC   pJC80 from pBR322
RC   pJC79 or pHC79 from pJC80 & Charon 3A
RC   pJB4 from pJC75-58
RA   Collins J.;
RT   "Escherichia coli plasmids packageable in vitro in bacteriophage
RT   particles";
RL   Meth. Enzymol. 68:309-326(1979).
XX
RN   [9]
RC   pJC703 from pBR322
RC   pJC720 from pBR322
RA   Collins J.;
RT   "Gene cloning with small plasmids";
RL   Curr. Top. Microbiol. Immunol. 78:122-170(1977).
XX
RN   [10]
RC   pJC74 from pJC703 & R1drd-19
RC   pJC75-49, pJC75-69, pJC75-40, pJC75-35, pJC75-65 from pJC74
RC   pJC75-58, pJC75-37 from pJC74
RC   pJC703delta from pJC703
RC   pJC720delta from pJC720
RA   Collins J., Bruning H.J.;
RT   "Plasmids useable as gene-cloning vectors in an in vitro packaging
RT   by coliphage lambda: cosmids";
RL   Gene 4:85-107(1978).
XX
CC   Boehringer size is 6524 bp.
CC   BRL size is 6523 bp; it is missing the c at 6481.
CC   ATCC size is 6430 bp.
CC   The lambda cohesive end site (cos region) is needed for packaging
CC   into pre-mature phage heads.
CC   pBR322 was partially digested with Sau3A and ligated with an 0.65
CC   BglII lambda fragment, generating a BglII site (pBR322 positions
CC   1459-1666 deleted).  A 1.65 kb BglII fragment from Charon 4A was
CC   ligated into the BglII site. [1]
CC   A general purpose cosmid vector derived from pBR322. [1]
CC   Medium is 1227 LB plus ampicillin.
CC   old Boehringer vector.
CC   NM (pHC79)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (cosmid)
CC   SP (BRL)(ATCC)
CC   HO (E.coli HB101)(E.coli LE392)(E.coli JM83)(E.coli 5K)(E.coli)
CC   CP ()
CC   FN (cloning 30000 to 40000 bp)(in vitro packaging)
CC   SE ()
CC   PA (pBR322)(lambda cos)
CC   BR ()
CC   OF (pV34)
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. lambda
FT                   -> plambda2H1
FT                   1. Charon 4A BglII-BglII 1650bp, lambda cos
FT                   2. plambda2H1 BglII
FT                   -> pBHB2830
FT                   1. pBR322 remove Sau3A/MboI-Sau3A/MboI 137bp
FT                   \ 4224bp
FT                   2. Charon 4A BglII-BglII 650bp, lambda cro/cII
FT                   -> pJC80 4874bp
FT                   1. pJC80 BglII 4874bp
FT                   2. pBHB2830 BglII-BglII 1650bp, lambda cos
FT                   -> pHC79 6524bp [unique EcaI at lambda cos]"
FT   misc_feature    1..1464
FT                   /note="from pBR322 bases 1..1464"
FT   misc_binding    23..23
FT                   /note="SIT unique ClaI"
FT   misc_binding    29..29
FT                   /note="SIT unique HindIII"
FT   CDS             86..1276
FT                   /note="ANT E. coli tetracycline resistance gene (tet)"
FT   misc_binding    375..375
FT                   /note="SIT unique BamHI"
FT   misc_binding    651..651
FT                   /note="SIT unique SalI"
FT   misc_feature    1461..2115
FT                   /note="from bacteriophage lambda bases
FT                   complement(38103..38758)"
FT   misc_feature    2115..3702
FT                   /note="from bacteriophage lambda bases
FT                   complement(1..1588)"
FT   misc_binding    >3240..<3500
FT                   /note="SIT lambda cohesive end site (cos)"
FT   misc_feature    3703..3839
FT                   /note="from bacteriophage lambda bases
FT                   complement(48366..48502)"
FT   misc_feature    3830..6524
FT                   /note="from pBR322 bases 1668..4363"
FT   rep_origin      complement(4700..4700)
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             complement(5458..6321)
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
FT   misc_binding    5774..5774
FT                   /note="SIT unique PstI"
FT   misc_binding    6522..6522
FT                   /note="SIT unique EcoRI"
FT   conflict        1761..1761
FT                   /note="G in pHC79; C in bacteriophage lambda bases
FT                   complement(38458..38458)"
FT   conflict        1972..1973
FT                   /note="GT in pHC79; GGT in bacteriophage lambda
FT                   bases complement(38245..38247)"
FT   conflict        2095..2095
FT                   /note="A in pHC79; C in bacteriophage lambda bases
FT                   complement(38123..38123)"
FT   conflict        2159..2159
FT                   /note="T in pHC79; G in bacteriophage lambda bases
FT                   complement(1544..1544)"
FT   conflict        2568..2568
FT                   /note="A in pHC79; T in bacteriophage lambda bases
FT                   complement(1135..1135)"
FT   conflict        2970..2970
FT                   /note="T in pHC79; A in bacteriophage lambda bases
FT                   complement(733..733)"
FT   conflict        3184..3184
FT                   /note="A in pHC79; T in bacteriophage lambda bases
FT                   complement(519..519)"
FT   conflict        3283..3283
FT                   /note="G in pHC79; A in bacteriophage lambda bases
FT                   complement(420..420)"
FT   conflict        3829..3829
FT                   /note="A in pHC79; T in bacteriophage lambda bases
FT                   complement(48376..48376)"
FT   conflict        4547..4547
FT                   /note="T in pHC79; G in pBR322 bases 2385..2385"
FT   conflict        5344..5345
FT                   /note="TA in pHC79; TAA in pBR322 bases 3182..3184"
XX
SQ   Sequence 6524 BP; 1514 A; 1792 C; 1618 G; 1600 T; 0 other;
     ttctcatgtt tgacagctta tcatcgataa gctttaatgc ggtagtttat cacagttaaa
     ttgctaacgc agtcaggcac cgtgtatgaa atctaacaat gcgctcatcg tcatcctcgg
     caccgtcacc ctggatgctg taggcatagg cttggttatg ccggtactgc cgggcctctt
     gcgggatatc gtccattccg acagcatcgc cagtcactat ggcgtgctgc tagcgctata
     tgcgttgatg caatttctat gcgcacccgt tctcggagca ctgtccgacc gctttggccg
     ccgcccagtc ctgctcgctt cgctacttgg agccactatc gactacgcga tcatggcgac
     cacacccgtc ctgtggatcc tctacgccgg acgcatcgtg gccggcatca ccggcgccac
     aggtgcggtt gctggcgcct atatcgccga catcaccgat ggggaagatc gggctcgcca
     cttcgggctc atgagcgctt gtttcggcgt gggtatggtg gcaggccccg tggccggggg
     actgttgggc gccatctcct tgcatgcacc attccttgcg gcggcggtgc tcaacggcct
     caacctacta ctgggctgct tcctaatgca ggagtcgcat aagggagagc gtcgaccgat
     gcccttgaga gccttcaacc cagtcagctc cttccggtgg gcgcggggca tgactatcgt
     cgccgcactt atgactgtct tctttatcat gcaactcgta ggacaggtgc cggcagcgct
     ctgggtcatt ttcggcgagg accgctttcg ctggagcgcg acgatgatcg gcctgtcgct
     tgcggtattc ggaatcttgc acgccctcgc tcaagccttc gtcactggtc ccgccaccaa
     acgtttcggc gagaagcagg ccattatcgc cggcatggcg gccgacgcgc tgggctacgt
     cttgctggcg ttcgcgacgc gaggctggat ggccttcccc attatgattc ttctcgcttc
     cggcggcatc gggatgcccg cgttgcaggc catgctgtcc aggcaggtag atgacgacca
     tcagggacag cttcaaggat cgctcgcggc tcttaccagc ctaacttcga tcactggacc
     gctgatcgtc acggcgattt atgccgcctc ggcgagcaca tggaacgggt tggcatggat
     tgtaggcgcc gccctatacc ttgtctgcct ccccgcgttg cgtcgcggtg catggagccg
     ggccacctcg acctgaatgg aagccggcgg cacctcgcta acggattcac cactccaaga
     attggagcca atcaattctt gcggagaact gtgaatgcgc aaaccaaccc ttggcagaac
     atatccatcg cgtccgccat ctccagcagc cgcacgcggc gcatctcggg cagcgttggg
     tcctggccac gggtgcgcat gatctgccac attacgctcc tgtccggcaa agttacctct
     gccgaagttg agtatttttg ctgtatttgt cataatgact cctgttgata gatccagtaa
     tgacctcaga actccatctg gatttgttca gaacgctcgg ttgccgccgg gcgtttttta
     ttggtgagaa tcgcagcaac ttgtcgcgcc aatcgagcca tgtcgtcgtc aacgaccccc
     cattcaagaa cagcaagcag cattgagaac tttggaatcc agtccctctt ccacctgctg
     atctgcgact tatcaacgcc gacagcttcc gctgtcttct cagttccaag cattgcgatt
     ttgttaagca acgcactctc gattcgtaga gcctcgttgc gtttgtttgc acgaaccata
     tgtaagtatt tccttagata acaattgatt gaatgtatgc aaataaatgc atacaccata
     ggtgtggttt aatttgatgc cctttttcag ggctggaatg tgtaagagcg ggttatttat
     gctgttgttt ttttgttact cgggaagggc tttacctctt ccgcataaac gcttccatca
     gcgtttatag ttaaaaaaat ctttcggcct gcatgaatgg ccttgttgat cgcgatttga
     tatacgccga gatctaacgg gcatcagtaa agtccagctc ctgctggcgg atgacgcatg
     cattatgctc gcagagataa aacacgctgg aggggtcatc cggcgtccat ttgaggccaa
     acggcgtctc tttgtcgcca aatttaagat actgctcctc cccgcaatgc gggcaggcaa
     catgaaaacg cataaaatgc ggggattcac tggctgcacg ctcaatctga caggtgcctc
     tcacttttgg cgtggagcca cggatggact ttggccagac cgagccttca atacgcttgt
     cacccaggaa cgtcggagag ccttcctgtt caatatcatc atcaaaagca gcaagttcat
     cataacccgc cacatccacc gacttttcac ggtagttttt tgccgcttta ccgcccaggc
     accagaagcc acgcccatta gtgaaacgct tcatggtgag cgtgttaacc cggtgctttt
     tgccatacca cggggccagc gccagcagcg acggaatatc acgaatagtc ggctcaacgt
     gggttttcat aaagttctcg gcatcaccat ccgtcggcaa ccagataagg gtgttgcgct
     gcttatgctc tataaagtag gcataaacac ccagcagcat tttggaataa ccgacacggg
     cagacttcac cacattcacc tcacggatgt agtcgctgcc catcgcattc atgatggccc
     gctgaaaggg cagtgtttcc cagcgccctt cctggtatgc ggattctttc gggagatagt
     aattagcatc cgcccattca acggcggtct gtggctccgg cctgaacagt gagcgaagcc
     cggcgcggac aaaatgccgc agcctgttat cctgactgtt cgatatattc actcagcaac
     cccggtatca gttcatccag cgcggctgct ttgttcatgg ctttgatgat atcccgtttc
     aggaaatcaa catgtcggtt ttccagttcc ggaaaacgcc gctgcaccga cagggggagc
     ccgtcgagaa tactggcaat ttcacctgcg atccgcgaca gcacgaaagt acagaatgcg
     gttaccacca cttcagcgga gtctctggca ttcttcagtt cctgtgcgtc ggcctgcgca
     cgcgtaagtc gatggcgttc gtactcaata gttcctggct ggggatctgc ctcgctggcc
     tgccgcagtt cttcaacctc ccggcgcagc ttttcgttct caatttcagc atccctttcg
     gcataccatt ttatgacggc ggcagagtca taaagcacct cattaccctt gccaccgcct
     cgcagaacgg gcattccctg ttcctgccag ttctgaatgg tacggatact cgcaccgaaa
     atgtcagcca gctgcttttt gttgacttcc attgttcatt ccacggacaa aaacagagaa
     aggaaacgac agaggccaaa aagcctcgct ttcagcacct gtcgtttcct ttcttttcag
     agggtatttt aaataaaaac attaagttat gacgaagaag aacggaaacg ccttaaaccg
     gaaaattttc ataaatagcg aaaacccgcg aggtcgccgc cccgtaacct gtcggatcac
     cggaaaggac ccgtaaagtg ataatgatta tcatctacat atcacaacgt gcgtggaggc
     catcaaacca cgtcaaataa tcaattatga cgcaggtatc gtattaatag atctgcatcg
     caggatgctg ctggctaccc tgtggaacac ctacatctgt attaacgaag cgctggcatt
     gaccctgagt gatttttctc tggtcccgcc gcatccatac cgccagttgt ttaccctcac
     aacgttccag taaccgggca tgttcatcat cagtaacccg tatcgtgagc atcctctctc
     gtttcatcgg tatcattacc cccatgaaca gaaattcccc cttacacgga ggcatcaagt
     gaccaaacag gaaaaaaccg cccttaacat ggcccgcttt atcagaagcc agacattaac
     gcttctggag aaactcaacg agctggacgc ggatgaacag gcagacatct gtgaatcgct
     tcacgaccac gctgatgagc tttaccgcag ctgcctcgcg cgtttcggtg atgacggtga
     aaacctctga cacatgcagc tcccggagac ggtcacagct tgtctgtaag cggatgccgg
     gagcagacaa gcccgtcagg gcgcgtcagc gggtgttggc gggtgtcggg gcgcagccat
     gacccagtca cgtagcgata gcggagtgta tactggctta actatgcggc atcagagcag
     attgtactga gagtgcacca tatgcggtgt gaaataccgc acagatgcgt aaggagaaaa
     taccgcatca ggcgctcttc cgcttcctcg ctcactgact cgctgctctc ggtcgttcgg
     ctgcggcgag cggtatcagc tcactcaaag gcggtaatac ggttatccac agaatcaggg
     gataacgcag gaaagaacat gtgagcaaaa ggccagcaaa aggccaggaa ccgtaaaaag
     gccgcgttgc tggcgttttt ccataggctc cgcccccctg acgagcatca caaaaatcga
     cgctcaagtc agaggtggcg aaacccgaca ggactataaa gataccaggc gtttccccct
     ggaagctccc tcgtgcgctc tcctgttccg accctgccgc ttaccggata cctgtccgcc
     tttctccctt cgggaagcgt ggcgctttct catagctcac gctgtaggta tctcagttcg
     gtgtaggtcg ttcgctccaa gctgggctgt gtgcacgaac cccccgttca gcccgaccgc
     tgcgccttat ccggtaacta tcgtcttgag tccaacccgg taagacacga cttatcgcca
     ctggcagcag ccactggtaa caggattagc agagcgaggt atgtaggcgg tgctacagag
     ttcttgaagt ggtggcctaa ctacggctac actagaagga cagtatttgg tatctgcgct
     ctgctgaagc cagttacctt cggaaaaaga gttggtagct cttgatccgg caaacaaacc
     accgctggta gcggtggttt ttttgtttgc aagcagcaga ttacgcgcag aaaaaaagga
     tctcaagaag atcctttgat cttttctacg gggtctgacg ctcagtggaa cgaaaactca
     cgttagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt
     aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc
     aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg
     cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg
     ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc
     cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta
     ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg
     ttgccattgc tgcaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct
     ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta
     gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg
     ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga
     ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt
     gcccggcgtc aacacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca
     ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt
     cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt
     ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga
     aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt
     gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc
     gcacatttcc ccgaaaagtg ccacctgacg tctaagaaac cattattatc atgacattaa
     cctataaaaa taggcgtatc acgaggccct ttcgtcttca agaa
//