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ID   PHE3       preliminary; circular DNA; SYN; 4355 BP.
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AC   IG0056; ATCC37161;
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DT   02-NOV-1992 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHE3 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHE3 from pACYC184 & pHWO
RA   Hennecke H., Gunther I., Binder F.;
RT   "A novel cloning vector for the direct selection of recombinant DNA
RT   in E. coli";
RL   Gene 19:231-234(1982).
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CC   A plasmid vector for positive selection of recombinants. (ATCC staff)
CC   Medium is 1675 LB plus chloramphenicol (10 ug/ml).
CC   NM (pHE3)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli RR28)(E.coli)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pACYC184)(pHWO)
CC   BR ()
CC   OF ()
CC   OR ()
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FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pACYC184 remove HincII-BamHI 278bp
FT                   \ 1871..2149, tet gene/3967bp
FT                   2. pHWO SmaI-BamHI, pheS gene
FT                   -> pHE3 4355bp"
FT   CDS             0..0
FT                   /note="GEN E. coli pheS+ gene, from pHWO"
FT   rep_origin      0..0
FT                   /note="ORI E. coli miniplasmid p15A, from pACYC184"
FT   CDS             244..900
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   from pACYC184"
FT   CDS             complement(>3051..4425)
FT                   /note="ANT E. coli chloramphenicol acetyltransferase
FT                   gene (CAT) chloramphenicol resistance gene (Cmr),
FT                   from pACYC184"
FT   misc_binding    0..0
FT                   /note="SIT unique PstI"
FT   misc_binding    0..0
FT                   /note="SIT unique HincII"
FT   misc_binding    0..0
FT                   /note="SIT unique BamHI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII"
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SQ   Sequence 4355 BP; 296 A; 240 C; 269 G; 315 T; 3235 other;
     aataaataaa tcctggtgtc cctgttgata ccgggaagcc ctgggccaac ttttggcgaa
     aatgagacgt tgatcggcac gtaagaggtt ccaactttca ccataatgaa ataagatcac
     taccgggcgt attttttgag ttatcgagat tttcaggagc taaggaagct aaaatggaga
     aaaaaatcac tggatatacc accgttgata tatcccaatg gcatcgtaaa gaacattttg
     aggcatttca gtcagttgct caatgtacct ataaccagac cgttcagctg gatattacgg
     cctttttaaa gaccgtaaag aaaaataagc acaagtttta tccggccttt attcacattc
     ttgcccgcct gatgaatgct catccggaat tccgtatggc aatgaaagac ggtgagctgg
     tgatatggga tagtgttcac ccttgttaca ccgttttcca tgagcaaact gaaacgtttt
     catcgctctg gagtgaatac cacgacgatt tccggcagtt tctacacata tattcgcaag
     atgtggcgtg ttacggtgaa aacctggcct atttccctaa agggtttatt gagaatatgt
     ttttcgtctc agccaatccc tgggtgagtt tcaccagttt tgatttaaac gtggccaata
     tggacaactt cttcgccccc gttttcacca tgggcaaata ttatacgcaa ggcgacaagg
     tgctgatgcc gctggcgatt caggttcatc atgccgtctg tgatggcttc catgtcggca
     gaatgcttaa tgaattacaa cagtactgcg atgagtggca gggcggggcg taattttttt
     aaggcagtta ttggtgccct taaacgcctg gtgctacgcc tgaataagtg ataataagcg
     gatgaatggc agaaattcga aagcaaattc gacccggtcg tcggttcagg gcagggtcgt
     taaatagccg cttatgtcta ttgctggttt accggtttat tgactaccgg aagcagtgtg
     accgtgtgct tctcaaatgc ctgaggccag ttggtaatga ctccaactta ttnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnngg cgctnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnaagct tnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnngga tccnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnngtt aacnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
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     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnggt
     naccnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn ggtnaccnnn
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     nnnnnnnagc gctnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn
     nnnnnnnnnn nnnnnnnnnn nnnnnnnnnn nnnnn
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