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ID   PHG146     preliminary; circular DNA; SYN; 2685 BP.
XX
AC   IG9826;
XX
DT   01-JUL-1995 (Rel. 11, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
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DE   E. coli plasmid vector pHG146 - complete.
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KW   cloning vector.
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OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
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RN   [1]
RC   pHG134 from pBR322 & linker
RC   pHG138 from pUC8 & linker
RC   pHG140 from pUC8 & linker
RC   pHG144 from pBR322 & linker
RC   pHG146 from pUC8 & linkers
RC   pHG153 from pBR322 & linkers
RC   pHG155 from pHG153
RC   pHG161 from pBR322 & linker
RC   pHG163 from pHG155
RC   pHG165 from pHG163 & pHG146
RC   pHG171 from pHG165 & pUC9
RC   pHG173 from pHG165 & linker
RC   pHG175 from pBR322 & linkers
RC   pHG177 from pHG175
RC   pHG179 from pHG165 & pHG175
RC   pHG327 from pHG165 & pUC18
RC   pHG329 from pHG165 & pUC19
RC   pHG331 from pHG146 & pUC18
RC   pHG333 from pHG146 & pUC19
RC   pHG335 from pHG146 & pUC9
RA   Stewart G.S., Lubinsky-Mink S., Jackson C.G., Cassel A., Kuhn J.;
RT   "pHG165: a pBR322 copy number derivative of pUC8 for cloning and
RT   expression";
RL   Plasmid 15:172-181(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   NM (pHG146)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP ()
CC   HO (E.coli JM101)(E.coli)(E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 HaeII 2665bp 5..5, 5' to lacZ
FT                   HpaI linker 6bp gttaac
FT                   HaeII 2665bp 429..429
FT                   HpaI linker 6bp gttaac
FT                   -> pHG146 2677bp"
FT   -               1..4
FT                   /note="pUC8 1..4 4bp
FT                   HaeII = RGCGC^Y
FT                   \             gttaac"
FT   -               5..10
FT                   /note="gttaac 6bp
FT                   \  gttaac
FT                   HaeII = R GCGC^Y"
FT   -               11..438
FT                   /note="pUC8 1..428 428bp
FT                   HaeII = RGCGC^Y
FT                   \             gttaac"
FT   -               439..444
FT                   /note="gttaac 6bp
FT                   \  gttaac
FT                   HaeII = R GCGC^Y"
FT   -               445..2685
FT                   /note="pUC8 425..2665 2241bp"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 2685 BP; 658 A; 686 C; 676 G; 665 T; 0 other;
     gcgcgttaac gcgcccaata cgcaaaccgc ctctccccgc gcgttggccg attcattaat
     gcagctggca cgacaggttt cccgactgga aagcgggcag tgagcgcaac gcaattaatg
     tgagttagct cactcattag gcaccccagg ctttacactt tatgcttccg gctcgtatgt
     tgtgtggaat tgtgagcgga taacaatttc acacaggaaa cagctatgac catgattacg
     aattcccggg gatccgtcga cctgcagcca agcttggcac tggccgtcgt tttacaacgt
     cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
     gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
     ctgaatggcg aatggcgcgt taacgcgcct gatgcggtat tttctcctta cgcatctgtg
     cggtatttca caccgcatat ggtgcactct cagtacaatc tgctctgatg ccgcatagtt
     aagccagccc cgacacccgc caacacccgc tgacgcgccc tgacgggctt gtctgctccc
     ggcatccgct tacagacaag ctgtgaccgt ctccgggagc tgcatgtgtc agaggttttc
     accgtcatca ccgaaacgcg cgagacgaaa gggcctcgtg atacgcctat ttttataggt
     taatgtcatg ataataatgg tttcttagac gtcaggtggc acttttcggg gaaatgtgcg
     cggaacccct atttgtttat ttttctaaat acattcaaat atgtatccgc tcatgagaca
     ataaccctga taaatgcttc aataatattg aaaaaggaag agtatgagta ttcaacattt
     ccgtgtcgcc cttattccct tttttgcggc attttgcctt cctgtttttg ctcacccaga
     aacgctggtg aaagtaaaag atgctgaaga tcagttgggt gcacgagtgg gttacatcga
     actggatctc aacagcggta agatccttga gagttttcgc cccgaagaac gttttccaat
     gatgagcact tttaaagttc tgctatgtgg cgcggtatta tcccgtattg acgccgggca
     agagcaactc ggtcgccgca tacactattc tcagaatgac ttggttgagt actcaccagt
     cacagaaaag catcttacgg atggcatgac agtaagagaa ttatgcagtg ctgccataac
     catgagtgat aacactgcgg ccaacttact tctgacaacg atcggaggac cgaaggagct
     aaccgctttt ttgcacaaca tgggggatca tgtaactcgc cttgatcgtt gggaaccgga
     gctgaatgaa gccataccaa acgacgagcg tgacaccacg atgcctgtag caatggcaac
     aacgttgcgc aaactattaa ctggcgaact acttactcta gcttcccggc aacaattaat
     agactggatg gaggcggata aagttgcagg accacttctg cgctcggccc ttccggctgg
     ctggtttatt gctgataaat ctggagccgg tgagcgtggg tctcgcggta tcattgcagc
     actggggcca gatggtaagc cctcccgtat cgtagttatc tacacgacgg ggagtcaggc
     aactatggat gaacgaaata gacagatcgc tgagataggt gcctcactga ttaagcattg
     gtaactgtca gaccaagttt actcatatat actttagatt gatttaaaac ttcattttta
     atttaaaagg atctaggtga agatcctttt tgataatctc atgaccaaaa tcccttaacg
     tgagttttcg ttccactgag cgtcagaccc cgtagaaaag atcaaaggat cttcttgaga
     tccttttttt ctgcgcgtaa tctgctgctt gcaaacaaaa aaaccaccgc taccagcggt
     ggtttgtttg ccggatcaag agctaccaac tctttttccg aaggtaactg gcttcagcag
     agcgcagata ccaaatactg tccttctagt gtagccgtag ttaggccacc acttcaagaa
     ctctgtagca ccgcctacat acctcgctct gctaatcctg ttaccagtgg ctgctgccag
     tggcgataag tcgtgtctta ccgggttgga ctcaagacga tagttaccgg ataaggcgca
     gcggtcgggc tgaacggggg gttcgtgcac acagcccagc ttggagcgaa cgacctacac
     cgaactgaga tacctacagc gtgagctatg agaaagcgcc acgcttcccg aagggagaaa
     ggcggacagg tatccggtaa gcggcagggt cggaacagga gagcgcacga gggagcttcc
     agggggaaac gcctggtatc tttatagtcc tgtcgggttt cgccacctct gacttgagcg
     tcgatttttg tgatgctcgt caggggggcg gagcctatgg aaaaacgcca gcaacgcggc
     ctttttacgg ttcctggcct tttgctggcc ttttgctcac atgttctttc ctgcgttatc
     ccctgattct gtggataacc gtattaccgc ctttgagtga gctgataccg ctcgccgcag
     ccgaacgacc gagcgcagcg agtcagtgag cgaggaagcg gaaga
//