back Return to this vector's summary.
ID   PHG165     preliminary; circular DNA; SYN; 3375 BP.
XX
AC   ATCC37618;
XX
DT   01-JUL-1993 (Rel. 7, Created)
DT   01-JUL-1995 (Rel. 12, Last updated, Version 1)
XX
DE   E. coli plasmid vector pHG165 - complete.
XX
KW   cloning vector.
XX
OS   Cloning vector
OC   Artificial sequences; Cloning vehicles.
XX
RN   [1]
RC   pHG134 from pBR322 & linker
RC   pHG138 from pUC8 & linker
RC   pHG140 from pUC8 & linker
RC   pHG144 from pBR322 & linker
RC   pHG146 from pUC8 & linkers
RC   pHG153 from pBR322 & linkers
RC   pHG155 from pHG153
RC   pHG161 from pBR322 & linker
RC   pHG163 from pHG155
RC   pHG165 from pHG163 & pHG146
RC   pHG171 from pHG165 & pUC9
RC   pHG173 from pHG165 & linker
RC   pHG175 from pBR322 & linkers
RC   pHG177 from pHG175
RC   pHG179 from pHG165 & pHG175
RC   pHG327 from pHG165 & pUC18
RC   pHG329 from pHG165 & pUC19
RC   pHG331 from pHG146 & pUC18
RC   pHG333 from pHG146 & pUC19
RC   pHG335 from pHG146 & pUC9
RA   Stewart G.S., Lubinsky-Mink S., Jackson C.G., Cassel A., Kuhn J.;
RT   "pHG165: a pBR322 copy number derivative of pUC8 for cloning and
RT   expression";
RL   Plasmid 15:172-181(1986).
XX
CC   Created by Moore, July 1995, under contract with NCBI.
CC   Enzymes that cut only once (not including polylinker): AatII, AvaI,
CC   BalI, ScaI.
CC   The HaeII sites flanking lac in pUC8 were changed to HpaI sites.
CC   pBR322 was modified by removing PstI & HincII sites in ampR gene and
CC   changing the EcoRI to HpaI.  A HpaI cassette with lac & MCS was cloned
CC   at the HpaI of the modified pBR322.
CC   pHG165 enables selection for inserts on MacConkey agar.  The lac gene
CC   and multiple cloning sites are easily removed with a HpaI digest.
CC   Plasmid vector with the lac gene and polylinker of pUC8 but with copy
CC   number control of pBR322.
CC   Restriction digests of the clone give the following sizes (kb):
CC   HindIII--3.5; HpaI--3.0, about 0.5. (ATCC staff)
CC   Medium is 1227 LB plus ampicillin.
CC   NM (pHG165)
CC   CM (yes)
CC   NA (ds-DNA)
CC   TP (circular)
CC   ST ()
CC   TY (plasmid)
CC   SP (ATCC)
CC   HO (E.coli JM101)(E.coli)(E.coli JM series)
CC   CP ()
CC   FN (cloning)
CC   SE ()
CC   PA (pBR322)(pUC8)
CC   BR ()
CC   OF ()
CC   OR ()
XX
FH   Key             Location/Qualifiers
FH
FT   misc_feature    0..0
FT                   /note="1. pUC8 HaeII 2665bp 5..5, 5' to lacZ
FT                   HpaI linker 6bp gttaac
FT                   HaeII 2665bp 429..429
FT                   HpaI linker 6bp gttaac
FT                   -> pHG146 2665bp
FT                   1. pBR322 EcoRI 4361bp 4360..4360
FT                   HpaI linker 6bp gttaac
FT                   AvaI 4364bp 1426..1426
FT                   HpaI linker 6bp gttaac
FT                   -> pHG153 4367bp
FT                   1. pHG153 remove HpaI-HpaI 1427bp 4360..4361..1426,
FT                   \ tet gene/2940bp
FT                   -> pHG155 2940bp
FT                   1. pHG155 remove BglI-XmnI 479bp, amp gene
FT                   \ pBR322 3489..3968/2460bp
FT                   2. pUC8 XmnI-BglI 482bp 1052..1534, amp gene
FT                   -> pHG163 2942bp [no PstI in amp, no HincII in amp]
FT                   1. pHG163 HpaI 2940bp 0..0
FT                   2. pHG146 HpaI-HpaI 424bp 5..429, lacZalpha
FT                   -> pHG165 3370bp"
FT   -               1..2063
FT                   /note="pBR322 1426..3488 2063bp
FT                   BglI = GCCNNNN^NGGC"
FT   -               2064..2542
FT                   /note="pUC8 1052..1530 479bp complement
FT                   XmnI = GAANN^NNTTC"
FT   -               2543..2938
FT                   /note="pBR322 3968..4361..2 396bp
FT                   EcoRI = G^AATT C
FT                   \              gttaac"
FT   -               2939..2944
FT                   /note="gttaac 6bp
FT                   \  gttaac
FT                   HaeII = R GCGC^Y"
FT   -               2945..3369
FT                   /note="pUC8 1..425 425bp
FT                   HaeII = RGCGC^Y
FT                   \             gttaac"
FT   -               3370..3375
FT                   /note="gttaac 6bp
FT                   \ gttaac
FT                   AvaI = C^YCGRG"
FT   misc_binding    0..0
FT                   /note="MCS HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT   misc_binding    0..0
FT                   /note="SIT unique HindIII-PstI-SalI-BamHI-SmaI-EcoRI"
FT   rep_origin      0..0
FT                   /note="ORI E. coli pMB1 (ColE1 and pBR322)"
FT   promoter        0..0
FT                   /note="PRO E. coli lac gene"
FT   CDS             0..0
FT                   /note="GEN E. coli beta-galactosidase gene (lacZ')"
FT   CDS             0..0
FT                   /note="ANT E. coli beta-lactamase gene (bla)
FT                   ampicillin resistance gene (apr/amp)"
XX
SQ   Sequence 3375 BP; 835 A; 890 C; 838 G; 812 T; 0 other;
     tcgggcagcg ttgggtcctg gccacgggtg cgcatgatcg tgctcctgtc gttgaggacc
     cggctaggct ggcggggttg ccttactggt tagcagaatg aatcaccgat acgcgagcga
     acgtgaagcg actgctgctg caaaacgtct gcgacctgag caacaacatg aatggtcttc
     ggtttccgtg tttcgtaaag tctggaaacg cggaagtcag cgccctgcac cattatgttc
     cggatctgca tcgcaggatg ctgctggcta ccctgtggaa cacctacatc tgtattaacg
     aagcgctggc attgaccctg agtgattttt ctctggtccc gccgcatcca taccgccagt
     tgtttaccct cacaacgttc cagtaaccgg gcatgttcat catcagtaac ccgtatcgtg
     agcatcctct ctcgtttcat cggtatcatt acccccatga acagaaatcc cccttacacg
     gaggcatcag tgaccaaaca ggaaaaaacc gcccttaaca tggcccgctt tatcagaagc
     cagacattaa cgcttctgga gaaactcaac gagctggacg cggatgaaca ggcagacatc
     tgtgaatcgc ttcacgacca cgctgatgag ctttaccgca gctgcctcgc gcgtttcggt
     gatgacggtg aaaacctctg acacatgcag ctcccggaga cggtcacagc ttgtctgtaa
     gcggatgccg ggagcagaca agcccgtcag ggcgcgtcag cgggtgttgg cgggtgtcgg
     ggcgcagcca tgacccagtc acgtagcgat agcggagtgt atactggctt aactatgcgg
     catcagagca gattgtactg agagtgcacc atatgcggtg tgaaataccg cacagatgcg
     taaggagaaa ataccgcatc aggcgctctt ccgcttcctc gctcactgac tcgctgcgct
     cggtcgttcg gctgcggcga gcggtatcag ctcactcaaa ggcggtaata cggttatcca
     cagaatcagg ggataacgca ggaaagaaca tgtgagcaaa aggccagcaa aaggccagga
     accgtaaaaa ggccgcgttg ctggcgtttt tccataggct ccgcccccct gacgagcatc
     acaaaaatcg acgctcaagt cagaggtggc gaaacccgac aggactataa agataccagg
     cgtttccccc tggaagctcc ctcgtgcgct ctcctgttcc gaccctgccg cttaccggat
     acctgtccgc ctttctccct tcgggaagcg tggcgctttc tcatagctca cgctgtaggt
     atctcagttc ggtgtaggtc gttcgctcca agctgggctg tgtgcacgaa ccccccgttc
     agcccgaccg ctgcgcctta tccggtaact atcgtcttga gtccaacccg gtaagacacg
     acttatcgcc actggcagca gccactggta acaggattag cagagcgagg tatgtaggcg
     gtgctacaga gttcttgaag tggtggccta actacggcta cactagaagg acagtatttg
     gtatctgcgc tctgctgaag ccagttacct tcggaaaaag agttggtagc tcttgatccg
     gcaaacaaac caccgctggt agcggtggtt tttttgtttg caagcagcag attacgcgca
     gaaaaaaagg atctcaagaa gatcctttga tcttttctac ggggtctgac gctcagtgga
     acgaaaactc acgttaaggg attttggtca tgagattatc aaaaaggatc ttcacctaga
     tccttttaaa ttaaaaatga agttttaaat caatctaaag tatatatgag taaacttggt
     ctgacagtta ccaatgctta atcagtgagg cacctatctc agcgatctgt ctatttcgtt
     catccatagt tgcctgactc cccgtcgtgt agataactac gatacgggag ggcttaccat
     ctggccccag tgctgcaatg ataccgcgag acccacgctc accggctcca gatttatcag
     caataaacca gccagccgga agggggccga gcgcagaagt ggtcctgcaa ctttatccgc
     ctccatccag tctattaatt gttgccggga agctagagta agtagttcgc cagttaatag
     tttgcgcaac gttgttgcca ttgctacagg catcgtggtg tcacgctcgt cgtttggtat
     ggcttcattc agctccggtt cccaacgatc aaggcgagtt acatgatccc ccatgttgtg
     caaaaaagcg gttagctcct tcggtcctcc gatcgttgtc agaagtaagt tggccgcagt
     gttatcactc atggttatgg cagcactgca taattctctt actgtcatgc catccgtaag
     atgcttttct gtgactggtg agtactcaac caagtcattc tgagaatagt gtatgcggcg
     accgagttgc tcttgcccgg cgtcaatacg ggataatacc gcgccacata gcagaacttt
     aaaagtgctc atcattggaa aattcttcgg ggcgaaaact ctcaaggatc ttaccgctgt
     tgagatccag ttcgatgtaa cccactcgtg cacccaactg atcttcagca tcttttactt
     tcaccagcgt ttctgggtga gcaaaaacag gaaggcaaaa tgccgcaaaa aagggaataa
     gggcgacacg gaaatgttga atactcatac tcttcctttt tcaatattat tgaagcattt
     atcagggtta ttgtctcatg agcggataca tatttgaatg tatttagaaa aataaacaaa
     taggggttcc gcgcacattt ccccgaaaag tgccacctga cgtctaagaa accattatta
     tcatgacatt aacctataaa aataggcgta tcacgaggcc ctttcgtctt caagaattgt
     taacgcgccc aatacgcaaa ccgcctctcc ccgcgcgttg gccgattcat taatgcagct
     ggcacgacag gtttcccgac tggaaagcgg gcagtgagcg caacgcaatt aatgtgagtt
     agctcactca ttaggcaccc caggctttac actttatgct tccggctcgt atgttgtgtg
     gaattgtgag cggataacaa tttcacacag gaaacagcta tgaccatgat tacgaattcc
     cggggatccg tcgacctgca gccaagcttg gcactggccg tcgttttaca acgtcgtgac
     tgggaaaacc ctggcgttac ccaacttaat cgccttgcag cacatccccc tttcgccagc
     tggcgtaata gcgaagaggc ccgcaccgat cgcccttccc aacagttgcg cagcctgaat
     ggcgaatggg ttaac
//